LOCUS       sequence-408931-       16513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16513)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2699
FEATURES             Location/Qualifiers
     source          1..16513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4113..4122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    12370..12958
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12423..12443)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12841..12852)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13030..13263
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13341..13462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13378..13397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13432..13451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13502..13637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13657..13676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13658..13676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13686..13703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13686..13702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13695..13717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13844..14299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13931..13950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14141..14162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14325..14429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14430..14498,14499..15290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14648..14667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15461..16049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15950..15969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16203..16220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16337..16358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16373..16378,16379..16396,16397..16403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16411..16427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16416..16438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16435..16451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16435..16451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16470..16490
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16472..16490
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gcggcggcgg gcggcggcag
     4141 ttgccccggg cctggctccg cgcggggccg cttcccgggc cggccgcggg gcgccggcgg
     4201 gggcgggggc cgcggcggac ggggcaacgg ggccgaaaga gtgcgggtag ctctgcggcg
     4261 cggcggtggc gcgacggggc cgggcggagc cgagcccggg gaggacacgg ccctgctccg
     4321 tttgctgggg ctccgccggg gcctgcgccg gctccgccgc ctgtgggccg gcccgcgggt
     4381 ccagcggggc cggggacggg gtcggggccg gggctggggc ccgagtcgag gctgcgtgcc
     4441 ggaggaggag agcagtgacg gggaatccga cgaggaggag tttcagggtt ttcattcaga
     4501 tgaagatgtg gcccccagtt ccctgcgctc tgcgctccga tcccagcgag gtcgagcgcc
     4561 ccgaggtcgg ggtcgcaagc ataagacgac cccccttcct cctcctcgcc tagcagatgt
     4621 ggctcctacc cccccaaaga cccctgcccg gaaacggggt gaggaaggca cagaacggat
     4681 ggtgcaggca ctgactgaac ttctccggcg ggcccaggca ccccaagcac cccggagccg
     4741 ggcatgtgag ccctccaccc cccggcggtc tcggggacgg cccccaggac ggccagcagg
     4801 cccctgcagg aggaagcagc aagcagtagt ggtggcagaa gcagctgtga caatccccaa
     4861 acctgagccc ccacctcctg tggttccagt gaaacatcag actggcagct ggaaatgcaa
     4921 ggaggggccc ggtccaggac ctgggacccc caggcgtgga ggacagtcaa gccgtggagg
     4981 ccgtggaggc aggggccgcg gccgaggtgg tgggctcccc tttgtgatca agtttgtttc
     5041 aagggccaaa aaagtaaaga tgggacaatt gtccttggga ctcgaatcag gtcaaggtca
     5101 aggtcaacat gaggaaagtt ggcaggatgt cccccaaaga agagttggat ctggacaggg
     5161 agggagccct tgctggaaaa agcaggaaca gaagctggat gacgaggaag aagagaagaa
     5221 agaagaagaa gaaaaagaca aggagggaga agagaaggaa gaaagagctg tagctgagga
     5281 gatgatgcca gctgcggaaa aggaagaggc aaagctgcca ccaccgcctc tgactcctcc
     5341 agccccttca cctcctccac ccctcccacc cccttcgaca tctcctccac ccccactctg
     5401 ccctccacca ccacccccag tgtccccacc acctctacca tcccctccac cgcctcctgc
     5461 ccaagaggag caggaggaat cccctcctcc tgtggtccca gctacgtgct ccaggaagag
     5521 gggccggcct cccctgactc ccagccagcg ggcggagcgg gaagctgctc gggcagggcc
     5581 agagggcacc tctcctccca ctccaacccc cagcaccgcc acgggaggcc ctccggaaga
     5641 cagtcccacc gtggccccca aaagcaccac cttcctgaag aatatccggc agtttattat
     5701 gcctgtggtg agtgcccgct cctcccgtgt catcaagaca ccccggcgat ttatggatga
     5761 agaccccccc aaacccccaa aggtggaggt ctcacctgtc ctgcgacctc ccattaccac
     5821 ctccccacct gttccccagg agccagcacc agtcccctct ccaccacgtg ccccaactcc
     5881 tccatctacc ccagttccac tccctgagaa gagacggtcc atcctaaggg aacccacatt
     5941 tcgctggacc tcactgaccc gggagctgcc ccctcctccc ccagcccctc cacctccccc
     6001 ggccccctcc ccaccccctg ctcctgccac ctcctcccgg aggcccctac tccttcgggc
     6061 ccctcagttt accccaagcg aagcccacct gaagatctac gaatcggtgc ttactcctcc
     6121 tcctcttggg gctcctgaag cccctgagcc agagcctcct cctgccgatg actctccagc
     6181 tgagcctgag cctcgggcag tgggccgcac caaccacctc agcctgcctc gattcgcccc
     6241 tgtggtcacc actcctgtta aggccgaggt gtcccctcac ggggctccag ctctgagcaa
     6301 cgggccacag acacaggctc agctactgca gcccctgcag gccttgcaaa cccagctcct
     6361 gccccaggca ctaccgccac cacagccaca gctgcagcca ccgccgtcac cacagcagat
     6421 gcctcccctg gaaaaagccc ggattgcggg cgtgggttcc ttgccgctgt ctggggtaga
     6481 ggagaagatg ttcagcctcc tcaagagagc caaagtgcag ctattcaaga tcgatcagca
     6541 gcagcagcag aaggtggcag cttccatgcc gctgagccct ggagggcaga tggaggaggt
     6601 ggccggggct gtcaagcaga tctccgacag aggccctgtc cggtctgaag atgagtcggt
     6661 ggaagctaag agagagcggc cctcaggtcc cgagtcccct gtgcaaggtc cccgcatcaa
     6721 acatgtctgc cgtcatgctg ctgtggccct gggtcaggcc cgggccatgg tgcctgaaga
     6781 tgtccctcgc ctcagtgccc tccctctccg ggatcggcag gacctcgcca cagaggatac
     6841 atcatcggcg tccgagactg agagtgtccc gtcacggtcc cggcggggaa aggtggaggc
     6901 agcaggccct gggggagaat cagagcccac aggttctgga gggaccctgg cccacacacc
     6961 ccggcgctca ctgccctccc atcacggcaa gaagatgcgc atggctcgat gtggacactg
     7021 tcggggctgc ctacgtgtgc aggactgtgg gtcctgtgtc aactgcctag acaagcccaa
     7081 gtttgggggc cctaacacca agaagcagtg ctgtgtatac cggaagtgtg acaaaataga
     7141 ggctcggaag atggaacgac tggctaaaaa aggccggacg atagtgaaga cgctgttgcc
     7201 ctgggattcc gatgaatctc ctgaggcctc ccctggtcct ccaggcccac gccggggggc
     7261 gggagctggg gggccccggg aggaggtggt ggcccaccca gggcccgagg agcaggactc
     7321 cctcctgcag cgcaagtcag ctcggcgctg cgtcaaacag cgaccctcct atgatatctt
     7381 cgaggattcg gatgactcgg agcccggggg cccccctgct cctcggcgtc ggaccccccg
     7441 agaaaatgag ctgccactgc cagaacctga ggagcagagc cggccccgca aacctaccct
     7501 gcagcctgtg ttgcagctca aggcccgaag gcgcctggac aaggatgctt tggcccctgg
     7561 cccctttgct tcttttccca atggctggac tggaaagcag aagtctcccg atggtgtgca
     7621 ccgcgtccgt gtggatttta aggaggattg tgatttagag aacgtgtggc tgatgggggg
     7681 cctgagtgtg ctcacctctg tgccaggggg ccccccgatg gtgtgcttgc tgtgtgccag
     7741 caaaggactc cacgagctgg tgttctgtca agtctgctgt gacccattcc acccattctg
     7801 cctggaggag gccgagcggc ccctgcccca gcatcacgac acctggtgct gccgtcgctg
     7861 caaattctgc cacgtctgtg gacgcaaagg tcgtggatcc aagcacctcc tggagtgcga
     7921 gcgctgccgc catgcatacc acccggcctg tctggggccc agctatccaa cccgggccac
     7981 gcgcaaacgg cgccactgga tctgttcagc ctgtgtgcgc tgtaagagct gtggggcaac
     8041 tccaggcaag aactgggacg tcgagtggtc tggagattac agcctctgcc ccaggtgcac
     8101 ccagctatat gagaaaggaa actactgccc gatctgtaca cgctgctatg aagacaacga
     8161 ctatgagagc aagatgatgc agtgcgcaca gtgcgatcac tgggtgcatg ccaagtgcga
     8221 ggggctctca gatgaagact acgagatcct ttcaggactg ccagactcgg tgctgtacac
     8281 ctgcggaccg tgtgctgggg cagcgcagcc ccgctggcga gaggccctga gcggggccct
     8341 ccaggggggc ctgcgccagg tgctccaggg cctgctgagc tccaaggtgg tgggcccact
     8401 gctgctctgc acccagtgtg ggccagatgg gaagcaactg cacccaggac cctgcggcct
     8461 gcaagctgtg agtcagcgct tcgaggatgg ccactacaag tctgtgcaca gcttcatgga
     8521 ggacatggtg ggcatcctca tgcggcactc ggaggaggga gagaccccgg accgccgggc
     8581 tggaggccag atgaaggggc tcctgctgaa gctgctagaa tctgcgttcg gctggttcga
     8641 cgcccacgac cccaagtact ggcgacggag tacccggctg ccaaacggag tccttcccaa
     8701 tgcggtgttg cccccatccc tggatcatgt ctatgcgcag tggagacagc aggaaccaga
     8761 gaccccagaa tcagggcagc ctccagggga tccctcagca gcattccagg gcaaggatcc
     8821 ggctgccttc tcacacctgg aggacccccg tcagtgtgca ctctgcctca aatacgggga
     8881 tgcagactcc aaggaggcgg ggcggctctt gtacatcggg cagaacgagt ggacacacgt
     8941 caactgtgcc atctggtcgg cggaagtctt cgaggagaac gacggctccc tcaagaatgt
     9001 gcatgctgct gtggcccgag ggaggcagat gcgctgcgag ctctgcctga agcctggcgc
     9061 cacggtgggc tgctgcctgt cctcctgcct cagcaacttc cacttcatgt gtgcccgggc
     9121 cagctactgc atcttccagg atgacaagaa agtcttctgc cagaaacaca ctgatctcct
     9181 ggatggcaag gaaattgtga accccgatgg ttttgatgtt ctccgccgag tctatgtgga
     9241 cttcgagggc atcaacttca agcggaagtt cttgacgggg cttgaacccg atgccatcaa
     9301 cgtgctcatt ggttccatcc gcattgactc cctgggtact ctgtctgatc tctcggactg
     9361 cgagggacgg ctcttcccca ttggctacca gtgctcccgt ctgtactgga gcacagtgga
     9421 tgctcggagg cgctgctggt atcggtgccg aattctggag tatcggccat gggggccgag
     9481 ggaagagcca gctcacctgg aggctgcaga ggagaaccag accattgtgc acagccccgc
     9541 cccttcctca gagcccccag gtggtgagga ccccccactg gacacagatg ttcttgtccc
     9601 tggagctcct gagcgccact cgcccattca gaacctggac cctccactgc ggccagattc
     9661 aggcagcgcc cctcctccag ccccccgttc tttttcgggg gctcgaatca aagtgcccaa
     9721 ctactcgcca tcccggaggc ccttgggggg tgtctccttt ggccccctgc cctcccctgg
     9781 aagtccatct tcactgaccc accacatccc cacagtggga gacccggact tcccagctcc
     9841 ccccagacgt tcccgtcgtc ccagcccttt ggctcccagg ccgcctccat cacggtgggc
     9901 ctcccctcct ctaaaaacct cccctcagct cagggtgccc cctcctacct cagtcgtcac
     9961 agccctcaca cctacctcag gggagctggc tccccctggc ccggccccat ctccaccacc
    10021 ccctgaagac ctgggcccag acttcgagga catggaggtg gtgtcaggac tgagtgctgc
    10081 tgacctggac ttcgcggcca gcctgctggg gactgagccc ttccaggaag agattgtagc
    10141 cgctggggcc atggggagca gccacggggg cccgggggac agctccgagg aggagtccag
    10201 ccccacctcc cgctacatcc acttccctgt gactgtggtg tccgcccctg gtctggcccc
    10261 cagcgctacc cctggagccc cccgcattga acagctggac ggcgtggacg acggcactga
    10321 cagtgaggct gaggcggtgc agcagcctcg gggccagggc acgcctcctt cggggccagg
    10381 agtagtccgg gcaggggtcc ttggggctgc aggggacagg gcccggcctc ctgaggacct
    10441 gccatcggaa attgtggatt ttgtgttgaa gaacctaggg ggtcctgggg atggaggtgc
    10501 tggccctaga gaggagtcac tccccccggc gcctcccctg gctaatggca gccagccctc
    10561 ccaaggcctg accgccagcc cagctgaccc cacccgcaca tttgcctggc tcccaggggc
    10621 cccaggggtc cgggtgttaa gccttggccc tgcccctgag ccccccaaac ccgccacatc
    10681 caaaatcata cttgtcaaca agctggggca agtatttgtg aagatggctg gggagggtga
    10741 acctgtccca cccccagtga agcagccacc tttgcccccc accatttccc ccacggctcc
    10801 cacctcctgg actctgcccc caggccccct cctcggcgtg ctgcccgtgg tcggagtggt
    10861 ccgccctgcc ccgcccccgc caccccctcc cctgacgctg gtgctgagca gtgggccagc
    10921 cagcccgccc cgccaggcca tccgcgtcaa gagggtgtcc actttctccg gccggtcccc
    10981 gccagcacct cccccataca aagccccccg gctggatgaa gatggagagg cctcagagga
    11041 tacccctcag gttccagggc ttggcagtgg cgggtttagc cgtgtgagga tgaaaacccc
    11101 cacagtgcgt ggggtccttg acctggatcg gcctggggag cccgctgggg aagaaagtcc
    11161 tgggcccctc caggaacggt cccctttgct gccacttccg gaagatggtc ctccccaggt
    11221 ccccgatggt cccccagacc tgctgcttga gtcccagtgg caccactatt caggtgaggc
    11281 ttcgagctct gaggaagagc ctccatcccc agatgataaa gagaaccagg ccccaaaacg
    11341 gactggccca catctgcgct tcgagatcag cagtgaggat gggttcagcg ttgaggcaga
    11401 gagcttggag ggggcgtgga gaactctgat cgagaaagtg caagaggccc gagggcatgc
    11461 ccgactcaga catctctcct ttagtggaat gagtggggcg agactcctgg gcatccacca
    11521 tgatgctgtc atcttcctgg ccgagcagct ccccggagcc cagcgttgcc agcactataa
    11581 gttccgttac caccagcagg gagagggcca ggaggagccg cccctgaatc cccatggggc
    11641 tgctcgggca gaggtctatc tccggaagtg cacctttgac atgttcaact tcctggcctc
    11701 ccagcaccgg gtgctccctg agggggccac ctgtgatgag gaagaggatg aggtgcagct
    11761 caggtcaacc agacgtgcca ccagcctgga gctgcccatg gccatgcgtt ttcgtcacct
    11821 taagaagacg tccaaagaag ctgtgggtgt ctacagatca gccatccacg ggcgaggcct
    11881 gttctgtaag cgcaacatcg acgcggggga gatggtcatc gagtactctg gcattgtcat
    11941 ccgctcggtg ttgactgaca agcgggagaa gttctacgat gggaagggca tcgggtgcta
    12001 tatgttccgc atggatgact ttgatgtagt ggacgccacg atgcatggca atgccgcccg
    12061 cttcatcaac cactcctgtg agcccaactg cttctctcgg gtcatccacg tggagggcca
    12121 gaaacacatt gttatcttcg ccctgcgccg catcctgcgt ggtgaggagc tcacctacga
    12181 ctacaagttc cccatcgagg atgccagcaa caagctgccc tgcaactgtg gcgccaagcg
    12241 ctgccgtcgg ttccttaact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
    12301 ttggctttat atatcttgtg gaaaggacga aaggagtgtg ccgactgact atcaacgcgt
    12361 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    12421 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    12481 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    12541 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    12601 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    12661 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    12721 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    12781 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    12841 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    12901 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
    12961 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    13021 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    13081 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    13141 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    13201 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    13261 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    13321 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    13381 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    13441 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    13501 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    13561 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    13621 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
    13681 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    13741 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    13801 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    13861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    13921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    13981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    14041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    14101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    14161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    14221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    14281 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    14341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    14401 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    14461 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    14521 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    14581 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    14641 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    14701 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    14761 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    14821 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    14881 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    14941 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    15001 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    15061 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    15121 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    15181 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    15241 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    15301 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    15361 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    15421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    15481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    15541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    15601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    15661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    15721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    15781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    15841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    15901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    15961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    16021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    16081 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    16141 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    16201 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    16261 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    16321 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    16381 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    16441 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    16501 tggagctgca agc
//
