LOCUS       sequence-408933-        9634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0767
FEATURES             Location/Qualifiers
     source          1..9634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..252
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             253..433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    480..605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1098..1331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1516..1560
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1791..2001
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1813..2009
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1860..1995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2041..2643
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2041..2060)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2537..2557
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2686..2803
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2941..4119
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3172..4110
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4067..4087
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4130..4139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5512..6100
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5565..5585)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5983..5994)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6172..6405
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6483..6604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6520..6539)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6574..6593
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6644..6779
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6799..6818)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6800..6818)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6828..6845)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6828..6844)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6837..6859)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6986..7441
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7073..7092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7283..7304
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7467..7571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7572..7640,7641..8432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7790..7809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8603..9191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9092..9111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9345..9362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9479..9500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9515..9520,9521..9538,9539..9545)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9553..9569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9558..9580
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9577..9593
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9577..9593
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9612..9632
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9614..9632
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
       61 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      121 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      181 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
      241 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      301 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      361 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      421 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      481 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      541 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      601 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
      661 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
      721 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
      781 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
      841 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
      901 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
      961 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1021 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1081 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1141 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1201 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1261 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1321 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1381 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1441 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1501 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1561 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1621 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     1681 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat
     1741 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     1801 gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca
     1861 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     1921 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     1981 gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc ggagcttacc
     2041 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     2101 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     2161 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     2221 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
     2281 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     2341 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     2401 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     2461 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     2521 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     2581 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccagt
     2641 taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2701 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2761 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2821 cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg gataaagttt
     2881 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
     2941 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     3001 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3061 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3121 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     3181 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     3241 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     3301 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3361 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3421 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3481 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     3541 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3601 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3661 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3721 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3781 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3841 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3901 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3961 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     4021 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     4081 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     4141 ggagacgaag gacgcagcgc agatgttggt gaccttcaag gatgtggctg tgacctttac
     4201 ccgggaggag tggagacagc tggacctggc ccagaggacc ctgtaccgag aggtgatgct
     4261 ggagacctgt gggcttctgg tttcactagg gcatcgggtt cccaaaccag agttggtcca
     4321 cctgctagag catgggcagg agctgtggat agtgaagaga ggcctctcac atgctacctg
     4381 tgcaggtgac agagcacaag ttcataccag agagccaacc acttacccgc cagtcttatc
     4441 tgagcgggcc tttctccggg gaagcctgac tctggaatct tcaacctcaa gtgattcgag
     4501 gttggggaga gctagggatg aggaagggct tttggaaatg cagaaaggta aagtgacacc
     4561 ggagacagac ctccacaagg agacccatct tgggaaagtg agccttgaag gtgaaggttt
     4621 ggggacagat gatggtctgc actccagggc attacaagag tggttatcag cagatgttct
     4681 ccatgaatgt gactcacagc aaccagggaa agacgccttg attcatgcag ggacaaaccc
     4741 ctacaaatgc aagcagtgtg ggaaaggttt taacaggaag tggtatctcg ttcgacatca
     4801 gagggttcac actggaatga aaccctatga atgcaatgca tgtgggaaag cctttagcca
     4861 gagctcaact ctcattcggc actacctcat ccacaccggg gagaaaccat acaagtgcct
     4921 tgagtgtggg aaggccttca aacgcaggtc gtacctcatg cagcaccatc caatccacac
     4981 tggagagaaa ccctatgagt gtagtcaatg tcgaaaggcc ttcacccacc ggtctacttt
     5041 tattcgccat aataggaccc acactggaga aaagcccttt gagtgcaaag aatgtgagaa
     5101 agcctttagc aatagagccc acctaattca acattacatc atccacactg gagagaagcc
     5161 ctatgattgc atggcatgtg gaaaggcctt cagatgcagc tcagaactca tacagcacca
     5221 gcggattcac actggggaga agccctatga atgcacccag tgtgggaaag cctttcaccg
     5281 gagcacgtac ctcattcagc actctgtcat ccacactggg gagatgccct acaagtgtat
     5341 cgagtgtggg aaggccttca aacgcaggtc acaccttctg cagcaccaaa gggtccatac
     5401 atagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     5461 tggaaaggac gagaagacat agctattgtt gagagaacgc gttaagtcga caatcaacct
     5521 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5581 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5641 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5701 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5761 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5821 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5881 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5941 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6001 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6061 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     6121 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6181 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6241 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6301 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6361 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     6421 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6481 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6541 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6601 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6661 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6721 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6781 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     6841 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6901 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6961 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7021 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7081 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7141 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7201 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7261 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7321 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7381 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7441 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7501 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7561 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7621 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7681 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7741 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7801 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7861 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7921 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7981 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8041 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8101 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8161 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8221 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8281 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8341 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8401 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8461 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8521 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8581 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8641 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8701 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8761 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8821 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8881 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8941 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9001 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9061 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9181 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9241 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9301 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9361 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9421 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9481 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9541 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9601 acgccaagcg cgcaattaac cctcactaaa ggga
//
