LOCUS       sequence-408934-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0770
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..237
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             238..418
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    465..590
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1083..1316
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1501..1545
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1776..1986
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1798..1994
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1845..1980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2026..2628
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2026..2045)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2522..2542
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2671..2788
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2926..4104
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3157..4095
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4052..4072
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4115..4124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5032..5620
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5085..5105)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5503..5514)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5692..5925
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6003..6124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6040..6059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6094..6113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6164..6299
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6319..6338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6320..6338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6348..6365)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6348..6364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6357..6379)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6506..6961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6593..6612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6803..6824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6987..7091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7092..7160,7161..7952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7310..7329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8123..8711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8612..8631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8865..8882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8999..9020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9035..9040,9041..9058,9059..9065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9073..9089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9078..9100
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9097..9113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9097..9113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9132..9152
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9134..9152
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
       61 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
      121 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
      181 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      421 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      481 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      541 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      601 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      661 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      721 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      781 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      841 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      901 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      961 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1021 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1081 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1141 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1201 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1261 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1321 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1381 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1441 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1501 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1561 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1621 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1681 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt
     1741 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     1801 gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     1861 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     1921 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     1981 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag
     2041 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     2101 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     2161 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     2221 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     2281 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     2341 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     2401 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     2461 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     2521 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     2581 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat
     2641 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2701 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2761 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     2821 ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat
     2881 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     2941 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     3001 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     3061 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     3121 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     3181 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     3241 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     3301 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     3361 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     3421 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     3481 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     3541 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3601 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     3661 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     3721 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3781 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3841 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3901 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3961 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     4021 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     4081 tttcttccat ttcaggtgtc gtgaggctag caccgccacc atggcttctc cgtccaaagg
     4141 caatgacttg ttttcgcccg acgaggaggg cccagcagtg gtggccggac caggcccggg
     4201 gcctgggggc gccgaggggg ccgcggagga gcgccgcgtc aaggtctcca gcctgccctt
     4261 cagcgtggag gcgctcatgt ccgacaagaa gccgcccaag gaggcgtccc cgctgccggc
     4321 cgaaagcgcc tcggccgggg ccaccctgcg gccactgctg ctgtcggggc acggcgctcg
     4381 ggaagcgcac agccccgggc cgctggtgaa gcccttcgag accgcctcgg tcaagtcgga
     4441 aaattcagaa gatggagcgg cgtggatgca ggaacccggc cgatattcgc cgccgccaag
     4501 acatatgagc cctaccacct gcaccctgag gaaacacaag accaatcgga agccgcgcac
     4561 gccctttacc acatcccagc tcctcgccct ggagcgcaag ttccgtcaga aacagtacct
     4621 ctccattgca gagcgtgcag agttctccag ctctctgaac ctcacagaga cccaggtcaa
     4681 aatctggttc cagaaccgaa gggccaaggc gaaaagactg caggaggcag aactggaaaa
     4741 gctgaaaatg gctgcaaaac ctatgctgcc ctccagcttc agtctccctt tccccatcag
     4801 ctcgcccctg caggcagcgt ccatatatgg agcatcctac ccgttccata gacctgtgct
     4861 tcccatcccg cctgtgggac tctatgccac gccagtggga tatggcatgt accacctgtc
     4921 ctagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     4981 tggaaaggac gaccggtctt acaagaggaa actagaacgc gttaagtcga caatcaacct
     5041 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5101 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5161 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5221 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5281 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5341 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5401 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5461 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5521 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5581 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     5641 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5701 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     5761 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5821 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5881 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     5941 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6001 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6061 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6121 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6181 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6241 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6301 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     6361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6481 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6781 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6841 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6901 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6961 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8941 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9001 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9061 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9121 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagct
//
