LOCUS       sequence-408938-       11642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human AAVS1-targeting plasmid for expression of mouse CR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1
FEATURES             Location/Qualifiers
     source          1..11642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..63
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(101..123)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     223..242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     436..458
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     450..467
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     451..467
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1904..3082
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2135..3073
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3030..3050
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3099..3118
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3099..3117
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7443..7508
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(8323..8340)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8329..8553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9493..9509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9493..9509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9506..9528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9517..9533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9541..9547,9548..9565,9566..9571))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9586..9607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9724..9741)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9895..10483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9975..9994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10654..11445,11446..11514))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11277..11296
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11515..11619)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
       61 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      121 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      181 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
      241 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      301 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      361 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      421 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgccgtga
      481 cgtcagcacg ccgggcgggg accgggagat ccttggggcg gtggggggcc agcggcagtt
      541 cccggcggcc cccggggcgg gcgggcgggc gggtggtggc ggcggttggg gctcggcgct
      601 cgctcgctcg ctgggcgggc gggcggtgcg atgtccggag aggatggccc ggcggctggc
      661 ccgggggcgg cggcggcggc tgcccgggag cggcgacggg agcagctgcg gcagtggggg
      721 gcgcgggcgg gcgccgagcc tggccccgga gagcgccgcg cccgcaccgt ccgcttcgag
      781 cgcgccgccg agttcctggc ggcctgtgcg ggcggcgacc tggacgaggc gcgtctgatg
      841 ctgcgcgccg ccgaccctgg ccccggcgcc gagctcgacc ccgccgcgcc gccgcccgcc
      901 cgcgccgtgc tggactccac caacgccgac ggtatcagcg ccctgcacca ggtcagcgcc
      961 ccccgcccgg cgtctcccgg ggccaggtcc accctctgct gcgccacctg gggcatcctc
     1021 cttccccgtt gccagtctcg atccgccccg tcgttcctgg ccctgggctt tgccacccta
     1081 tgctgacacc ccgtcccagt cccccttacc attccccttc gaccacccca cttccgaatt
     1141 ggagccgctt caactggccc tgggcttagc cactctgtgc tgaccactct gccccaggcc
     1201 tccttaccat tccccttaaa caccggtggg gaggtggtcc ctgctgttac gccaatgata
     1261 acccccgcca gaaaaatctt agtagccttc cctttttgtt ttccgtgccc caactcggcg
     1321 gattgactcg gccccttccg gaaacacccg aatcaacttc tagtcaaatt attgttcacg
     1381 ccgcaatgac ccacccctgg cccgcgtctg tggaactgac ccctggtgta caggagagtt
     1441 cgctgctgaa agtggtccca aaggggtact agtttttaag ctcccaactc cccctccccc
     1501 agcgtctgga ggattccaca ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt
     1561 tttggcgcca gccgctgagg ctgccaagca gaaaagccac cgctgaggag actccggtca
     1621 ctgtcctcgc cccgcctccc ccttccctcc ccttggggac caccgggcgc cacgccgcga
     1681 acgttaagtg ccgcggtcgt cggcgcctcc gccctccccc ttgggcccca attcccagcg
     1741 ggcgcggcgc gcggcccctc cccccgccgg gcgcgcgccc gctgccccgc ccttcgtggc
     1801 cgcccggcgt gggcggtgcc acccctcccc ccggcggccc cgcgcgcagc tcccggctcc
     1861 ctcccccttc ggatgtggct tgagctgtag gcgcggaggg ccgggctccg gtgcccgtca
     1921 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1981 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2041 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2101 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2161 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2221 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2281 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2341 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2401 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2461 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2521 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2581 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2641 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2701 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2761 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2821 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2881 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2941 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3001 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3061 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     3121 acccaagctg gctaggtaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     3181 attctgcaga tatccagcac agtggcacca tgggatcctt gggttcgctc tgggttttct
     3241 tcactctcat cactccagga gttcttggtc agtgtaagtt gctgccaaag tattcttttg
     3301 ctaaaccttc tattgtgagt gataaatctg agtttgccat tggaacaact tgggaataca
     3361 aatgtcgccc tgggtatttt aggaagtcat ttattatcac ctgcttagaa acctccaagt
     3421 ggtcagatgc tcagcagttc tgtaaacgta aaccatgtat gaatcctcaa gaacccctcc
     3481 atggttctgt gcatataaac acgggtatcg agtttgggtc aacaattacg tattcttgta
     3541 atcaaggata tcgactcatt ggtgactcgt ctgctacatg tattgtatca gacaatactg
     3601 taatgtggga taatgatatg cctctttgtg aatctattcc ttgtgagtca cctccagcca
     3661 tctccaatgg agacttctac agcagcagca gagacagctt tttctatggg atggtagtaa
     3721 cttattattg ccataccgga aagaataggg aaaaactgtt tgatctggtg ggtgagaagt
     3781 caatatattg taccagcaaa gacaatcaag ttggcatctg gaatagtcca cctcctcagt
     3841 gtattcctag agtcaagtgc ccaatgccag aaattgaaaa tggactagtg gagtctggat
     3901 ttaaacactc cttcttctta aatgatacag taatatttaa gtgcaaatct ggctttacca
     3961 tgaaaggcag cagaatagca tggtgccagc caaacagcaa atggagccct ccattgccaa
     4021 catgcttcat gggatgtcta ccacctcaaa atatcctcca tggtgattat aacaaaaagg
     4081 atgagttctt ctctgttggc cagaaagtgt catatagctg taaccctggc tatactctca
     4141 ttggaactaa cctcgtggag tgtacatcct tgggaacctg gagcaataca gtcccgacat
     4201 gtgaagtgaa atcatgtgat gcaattccaa accatcttct ccatggccgt gtgtttcttc
     4261 cccctaatct ccagcttggg gcagaggttt cctttgtttg tgacttaggg ttccagttaa
     4321 aaggcaaacc ttctagtcag tgtatcccag aaggagagac agtaatctgg aataataagt
     4381 ttcctgtctg tgaacagatt tcttgtgacc ctcctcctga agtcaaaaat gctcggaaac
     4441 cctattattc tcttcccata gttcctggaa ctgttctgag gtacacttgt tcacctagct
     4501 accgcctcat tggagaaaag gctatctttt gtataagtga aaatcaagtg catgccacct
     4561 gggataaagc tcctcctata tgtgaatctg tgaataaaac catttcttgc tcagatccca
     4621 tagtaccagg gggattcatg aataaaggat ctaaggcacc attcagacat ggtgattctg
     4681 tgacatttac ctgtaaagcc aacttcacca tgaaaggaag caaaactgtc tggtgccagg
     4741 caaatgaaat gtggggacca acagctctgc cagtctgtga gagtgatttc cctctggagt
     4801 gcccatcact tccaacgatt cataatggac accacacagg acagcatgtt gaccagtttg
     4861 ttgcggggtt gtctgtgaca tacagttgtg aacctggcta tttgctcact ggaaaaaaga
     4921 caattaagtg cttatcttca ggagactggg atggtgtcat cccgacatgc aaagaggccc
     4981 agtgtgaaca tccaggaaag tttcccaatg ggcaggtaaa ggaacctctg agccttcagg
     5041 ttggcacaac tgtgtacttc tcctgtaatg aagggtacca attacaagga caaccctcta
     5101 gtcagtgtgt aattgttgaa cagaaagcca tctggactaa gaagccagta tgtaaagaaa
     5161 ttctctgccc accacctcca cctgttcgta atggaagtca tacaggcagc ttttcagaaa
     5221 atgtaccata tggaagcaca gttacctaca cctgtgaccc aagcccagag aaaggcgtga
     5281 gcttcactct tattggagag aagactatca attgtactac tggtagtcag aagactggga
     5341 tctggagtgg ccctgctcca tattgtgtac tttcaacttc tgcagttctg tgtttacaac
     5401 cgaagatcaa aagagggcaa atattatcta ttttgaaaga tagttattca tataatgaca
     5461 ctgtggcatt ttcttgtgaa cctggcttca ccttgaaggg caacaggagc attcgatgca
     5521 atgctcatgg cacatgggag ccaccggtac cagtgtgtga aaaaggatgt caggctcctc
     5581 ctaaaattat caatgggcaa aaagaagata gttacttgct caactttgac cctggtacat
     5641 ccataagata tagctgtgac cctggctatt tactggtggg agaggacact atacattgca
     5701 cccctgaggg gaagtggaca cccattactc cccagtgcac agttgcagag tgtaagccag
     5761 taggaccaca tctctttaag aggcctcaga atcagtttat taggacagct gttaattctt
     5821 cttgtgatga agggttccag ttaagtgaga gtgcttatca actgtgtcaa ggtacaattc
     5881 cttggtttat agaaatccgt ctttgtaaag aaatcacctg cccaccacct cctgttatac
     5941 acaacgggac acatacatgg agttcctcag aagatgtccc atatggaact gtggtcacat
     6001 acatgtgcta tcctgggcca gaggaaggcg taaaattcaa actcatcggg gagcaaacca
     6061 tccactgtac aagtgacagc agaggaagag gctcctggag tagccctgct cctctctgta
     6121 aactttccct cccagctgtc cagtgcacag acgttcatgt tgaaaatgga gtcaagctca
     6181 ctgacaataa agccccatat ttctacaatg atagtgtgat gttcaagtgt gatgatggat
     6241 atattttgag tggaagcagt cagatccggt gtaaagccaa taatacctgg gatcctgaaa
     6301 aaccactttg taaaaaagaa ggatgtgagc ctatgagagt acatggcctt ccagatgatt
     6361 cacatataaa actagtgaaa agaacctgtc aaaatgggta ccagttgact ggatatactt
     6421 atgagaagtg tcaaaatgct gagaatggga cttggtttaa aaagattgaa gtttgtacag
     6481 ttattctctg tcaacctcca ccaaaaattg caaatggtgg tcacacaggc atgatggcaa
     6541 agcacttcct atatggaaat gaagtttctt atgaatgtga tgaagggttc tatcttttgg
     6601 gagagaaaag tttgcagtgc gtaaatgatt ctaaaggtca tggctcttgg agtggacctc
     6661 caccacaatg cttacaatct tctcctctaa ctcattgccc cgatccagaa gtcaaacatg
     6721 gttacaaact caataaaact cattctgcat tttctcataa tgacatagta cattttgtct
     6781 gcaatcaagg cttcatcatg aacggcagcc acttgataag gtgtcatact aataacacat
     6841 ggttaccagg tgtaccaact tgtatcagaa aggcttcttt agggtgtcag tctccatcca
     6901 caatccccaa tgggaatcat actggtggga gtatagctcg atttccccct ggaatgtcag
     6961 tcatgtacag ttgctaccaa ggcttcctta tggctggaga ggcacgtctt atctgtactc
     7021 atgagggtac ctggagtcaa cctccccctt tttgcaaaga ggtaaactgt agcttccctg
     7081 aagatacaaa tggaatccag aagggatttc aacctgggaa aacctatcga tttggggcta
     7141 ctgtgactct ggaatgtgag gatgggtata ccttggaggg aagtccccag agccagtgcc
     7201 aggatgacag ccaatggaac cctcccttgg ctctttgcaa ataccggtca actattcctc
     7261 ttatttgtgg tatttctgtg ggctcagcac ttatcatttt gatgagtgtc ggcttctgta
     7321 tgatattaaa acacagagaa agcaattatt atacaaagac aagacccaaa gaaggagctc
     7381 ttcatttaga aacacgagaa gtatattcta ttgatccata taacccagca agcggcagcg
     7441 gagtgaagca gaccctcaac ttcgatctcc tcaaactggc cggagacgtc gaaagcaatc
     7501 ctggacctat gatcgagcag gacggactcc atgctggaag ccctgccgct tgggtcgaga
     7561 ggctctttgg atacgactgg gctcagcaga caatcggctg cagcgatgcc gccgtgttta
     7621 gactcagcgc ccagggcaga cctgtgctgt ttgtgaagac cgacctgagc ggagctctca
     7681 atgagctcca agatgaagcc gccagactga gctggctcgc caccacaggc gtgccttgtg
     7741 ctgccgtgct ggacgtcgtc acagaggccg gaagggattg gctgctcctg ggcgaagtcc
     7801 ccggacagga tctgctgagc tcccatctgg cccctgccga aaaggtctcc attatggccg
     7861 atgccatgag gaggctgcac accctggatc ccgctacctg tccctttgac catcaggcca
     7921 agcacaggat tgagagggcc agaacaagaa tggaggccgg cctggtcgac caggatgatc
     7981 tggacgagga acaccagggc ctcgctcctg ccgagctctt tgccagactc aaggccagga
     8041 tgcctgacgg cgaagatctg gtggtgaccc atggagatgc ctgtctcccc aacatcatgg
     8101 tggaaaacgg caggttctcc ggattcattg actgcggcag gctcggcgtg gccgacaggt
     8161 atcaggacat cgccctggct accagagata tcgctgagga gctgggagga gagtgggccg
     8221 atagatttct cgtgctctat ggcattgccg ctcccgacag ccagagaatt gccttctaca
     8281 gactgctcga tgagtttttt tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     8341 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     8401 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     8461 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     8521 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     8581 gctctagggg gtatccccac gcgccctgta gcggcgcatc tagcggccga tcaggcccga
     8641 cctactctct tccgcattgg agtcgcttta actggccctg gctttggcag cctgtgctga
     8701 cccatgcagt cctccttacc atccctccct cgacttcccc tcttccgatg ttgagcccct
     8761 ccagccggtc ctggactttg tctccttccc tgccctgccc tctcctgaac ctgagccagc
     8821 tcccatagct cagtctggtc tatctgcctg gccctggcca ttgtcacttt gcgctgccct
     8881 cctctcgccc ccgagtgccc ttgctgtgcc gccggaactc tgccctctaa cgctgccgtc
     8941 tctctcctga gtccggacca ctttgagctc tactggcttc tgcgccgcct ctggcccact
     9001 gtttcccctt cccaggcagg tcctgctttc tctgacctgc attctctccc ctgggcctgt
     9061 gccgctttct gtctgcagct tgtggcctgg gtcacctcta cggctggccc agatccttcc
     9121 ctgccgcctc cttcaggttc cgtcttcctc cactccctct tccccttgct ctctgctgtg
     9181 ttgctgccca aggatgctct ttccggagca cttccttctc ggcgctgcac cacgtgatgt
     9241 cctctgagcg gatcctcccc gtgtctgggt cctctccggg catctctcct ccctcaccca
     9301 accccatgcc gtcttcactc gctgggttcc cttttccttc tccttctggg gcctgtgcca
     9361 tctctcgttt cttaggatgg ccttctccga cggatgtctc ccttgcgtcc cgcctcccct
     9421 tcttgtaggc ctgcatcagg ccaaattggc cgtcgactgc agaggcctgc atgcaagctt
     9481 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9541 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9601 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9661 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10081 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10141 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10201 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10261 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10321 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10381 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10441 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10501 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10561 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10621 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10681 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10741 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10801 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10861 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10921 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10981 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11041 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11101 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11161 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11221 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11281 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11341 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11401 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11461 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11521 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11581 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11641 ac
//
