LOCUS       sequence-408940-       12048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse 
            CD59, and Qa1 SCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 12048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2...
FEATURES             Location/Qualifiers
     source          1..12048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..63
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(101..123)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     223..242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     436..458
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     450..467
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     451..467
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1904..3082
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2135..3073
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3030..3050
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3099..3118
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3099..3117
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4420..4446
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4462..4518
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5146..5157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5848..5901
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6280..6339
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7849..7914
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(8729..8746)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8735..8959
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9899..9915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9899..9915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9912..9934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9923..9939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9947..9953,9954..9971,9972..9977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9992..10013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10130..10147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10301..10889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10381..10400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11060..11851,11852..11920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11683..11702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11921..12025)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
       61 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      121 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      181 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
      241 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      301 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      361 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      421 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgccgtga
      481 cgtcagcacg ccgggcgggg accgggagat ccttggggcg gtggggggcc agcggcagtt
      541 cccggcggcc cccggggcgg gcgggcgggc gggtggtggc ggcggttggg gctcggcgct
      601 cgctcgctcg ctgggcgggc gggcggtgcg atgtccggag aggatggccc ggcggctggc
      661 ccgggggcgg cggcggcggc tgcccgggag cggcgacggg agcagctgcg gcagtggggg
      721 gcgcgggcgg gcgccgagcc tggccccgga gagcgccgcg cccgcaccgt ccgcttcgag
      781 cgcgccgccg agttcctggc ggcctgtgcg ggcggcgacc tggacgaggc gcgtctgatg
      841 ctgcgcgccg ccgaccctgg ccccggcgcc gagctcgacc ccgccgcgcc gccgcccgcc
      901 cgcgccgtgc tggactccac caacgccgac ggtatcagcg ccctgcacca ggtcagcgcc
      961 ccccgcccgg cgtctcccgg ggccaggtcc accctctgct gcgccacctg gggcatcctc
     1021 cttccccgtt gccagtctcg atccgccccg tcgttcctgg ccctgggctt tgccacccta
     1081 tgctgacacc ccgtcccagt cccccttacc attccccttc gaccacccca cttccgaatt
     1141 ggagccgctt caactggccc tgggcttagc cactctgtgc tgaccactct gccccaggcc
     1201 tccttaccat tccccttaaa caccggtggg gaggtggtcc ctgctgttac gccaatgata
     1261 acccccgcca gaaaaatctt agtagccttc cctttttgtt ttccgtgccc caactcggcg
     1321 gattgactcg gccccttccg gaaacacccg aatcaacttc tagtcaaatt attgttcacg
     1381 ccgcaatgac ccacccctgg cccgcgtctg tggaactgac ccctggtgta caggagagtt
     1441 cgctgctgaa agtggtccca aaggggtact agtttttaag ctcccaactc cccctccccc
     1501 agcgtctgga ggattccaca ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt
     1561 tttggcgcca gccgctgagg ctgccaagca gaaaagccac cgctgaggag actccggtca
     1621 ctgtcctcgc cccgcctccc ccttccctcc ccttggggac caccgggcgc cacgccgcga
     1681 acgttaagtg ccgcggtcgt cggcgcctcc gccctccccc ttgggcccca attcccagcg
     1741 ggcgcggcgc gcggcccctc cccccgccgg gcgcgcgccc gctgccccgc ccttcgtggc
     1801 cgcccggcgt gggcggtgcc acccctcccc ccggcggccc cgcgcgcagc tcccggctcc
     1861 ctcccccttc ggatgtggct tgagctgtag gcgcggaggg ccgggctccg gtgcccgtca
     1921 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1981 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2041 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2101 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2161 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2221 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2281 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2341 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2401 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2461 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2521 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2581 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2641 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2701 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2761 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2821 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2881 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2941 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3001 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3061 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     3121 acccaagctg gctaggtaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     3181 attctgcaga tatccagcac agtggcggcc gccatgctgg agggcgtgca ggtcgagaca
     3241 atcagccctg gagacggaag gaccttcccc aaaagaggcc aaacatgcgt ggtccattac
     3301 accggaatgc tcgaggacgg caaaaaggtg gacagcagca gggataggaa caaacccttc
     3361 aaattcatgc tgggaaaaca agaggtgatc agaggatggg aggaaggagt ggcccaaatg
     3421 tccgtgggac agagggccaa gctgacaatc tcccccgatt acgcctacgg cgctacaggc
     3481 catcctggaa tcatcccccc ccacgccaca ctggtgttcg atgtggaact cctcaagctg
     3541 gagtccggag gaggctccgg agtggacgga tttggcgacg tgggagctct ggaaagcctc
     3601 agaggaaacg ccgacctcgc ctatattctg tccatggagc cctgtggcca ttgcctcatc
     3661 attaataacg tgaacttctg cagggaaagc ggactgagga caagaaccgg cagcaacatt
     3721 gactgtgaga aactgagaag gagattcagc tccctgcatt tcatggtgga ggtgaagggc
     3781 gatctgacag ccaagaaaat ggtcctggcc ctgctcgagc tggccagaca ggaccacgga
     3841 gctctggact gctgtgtggt cgtcatcctc agccacggat gccaagctag ccatctgcaa
     3901 ttccccggcg ctgtgtatgg aaccgatggc tgtcccgtgt ccgtggaaaa aatcgtcaat
     3961 atctttaacg gcacaagctg cccctccctc ggaggcaaac ctaagctgtt ctttattcaa
     4021 gcttgcggcg gcgagcaaaa ggaccatggc tttgaagtgg cttccacctc ccccgaagac
     4081 gagagccccg gatccaatcc tgagcccgat gctaccccct tccaggaggg cctcaggacc
     4141 ttcgaccagc tcgatgccat cagcagcctg cctacccctt ccgacatctt cgtgtcctac
     4201 agcacctttc ccggattcgt gagctggagg gatcccaaga gcggaagctg gtatgtggag
     4261 acactggacg atatttttga gcagtgggcc cacagcgagg atctgcaatc cctgctgctg
     4321 agagtcgcca acgccgtgag cgtgaaagga atctacaagc agatgcccgg atgcttcaac
     4381 ttcctgagga agaagctgtt cttcaagacc agcgtggact atccctatga tgtgcccgac
     4441 tacgccctcg atggctccgg agccaccaac ttcagcctgc tcaagcaagc cggcgacgtg
     4501 gaggagaacc ctggacctat ggaagtgtcc tccagatcca gcgagcctct ggatcccgtg
     4561 tggctcctgg tcgccttcgg aagaggaggc gtcaagctgg aggtcctgct gctgtttctg
     4621 ctccccttca cactgggcca ctgtcctgcc cctagccaac tgccctccgc caagcctatc
     4681 aacctcaccg atgagtccat gtttcccatt ggcacctacc tcctgtatga gtgcctccct
     4741 ggatatatta agagacagtt ctccatcaca tgcaagcagg attccacctg gaccagcgcc
     4801 gaagacaagt gcatcaggaa gcagtgcaaa accccttccg atcccgaaaa tggactcgtg
     4861 cacgtgcaca caggaatcca gttcggctcc agaattaatt atacatgtaa tcagggctac
     4921 agactgatcg gcagctccag cgctgtctgt gtgattaccg atcagagcgt ggattgggac
     4981 accgaggccc ctatctgtga gtggatccct tgcgagattc cccctggcat tcctaacggc
     5041 gacttcttta gctccaccag agaggatttc cactacggca tggtcgtcac ctacaggtgc
     5101 aacacagacg ccaggggaaa agccctgttc aacctggtcg gcgagccttc cctctattgc
     5161 acatccaatg acggcgaaat cggcgtctgg agcggacctc ccccccaatg catcgaactg
     5221 aacaagtgca cacccccccc ttatgtggag aatgccgtca tgctcagcga aaataggtcc
     5281 ctgttttccc tgagggacat cgtggagttc aggtgtcacc ctggcttcat catgaaggga
     5341 gccagcagcg tgcattgcca gagcctcaat aagtgggagc ccgagctgcc ctcctgcttc
     5401 aagggcgtca tttgcagact gccccaggag atgagcggct tccaaaaggg cctcggcatg
     5461 aagaaagagt actactatgg cgaaaatgtc accctggaat gcgaggatgg ctatacactc
     5521 gagggcagct cccagagcca gtgccagagc gatggcagct ggaaccctct cctggctaag
     5581 tgcgtgtcca ggagcatcag cggcctgatt gtgggcatct ttattggaat tattgtcttc
     5641 atcctcgtga tcattgtgtt tatctggatg attctgaagt acaaaaaaag aaacaccaca
     5701 gatgaaaagt ataaagaggt gggcattcat ctcaactaca aggaggatag ctgcgtgaga
     5761 ctgcagtccc tgctcacatc ccaagaaaat agcagcacca catcccccgc cagaaattcc
     5821 ctcacccaag aggtgtccgg aagcggcgag ggaaggggaa gcctcctgac atgcggcgat
     5881 gtggaagaaa atcccggccc tatgagggcc cagaggggcc tgatcctgct gctcctcctg
     5941 ctggccgtgt tctgcagcac agccgtgtcc ctcacctgct accattgctt ccagcctgtc
     6001 gtgtccagct gcaacatgaa tagcacatgc tcccctgacc aggattcctg cctgtacgct
     6061 gtcgctggaa tgcaggtgta ccagaggtgc tggaagcaaa gcgactgtca tggcgagatc
     6121 atcatggatc agctggagga gaccaagctg aagttcagat gttgtcagtt taatctctgt
     6181 aacaagagcg atggaagcct cggaaaaacc cctctcctcg gaacaagcgt gctcgtcgcc
     6241 attctgaatc tctgctttct gtcccatctg ggatccggcc aatgcaccaa ctatgccctc
     6301 ctcaaactcg ccggagatgt ggaatccaac cccggcccta tggctaggtc cgtcaccctg
     6361 gtcttcctgg tgctggtgag cctgaccggc ctgtatgctg ctatggctcc caggacactc
     6421 ctgctgggct gtggagcctc cggaggaggc ggcagcggag gcggcggctc cattcagaag
     6481 acacctcaaa tccaggtgta ctccagacac ccccccgaga acggaaagcc taatattctc
     6541 aattgttacg tgacccagtt tcaccccccc catattgaga tccagatgct caaaaacggc
     6601 aagaaaattc ctaaggtgga gatgagcgac atgagcttta gcaaggactg gagcttctac
     6661 attctcgccc acacagaatt tacacccacc gagaccgaca cctatgcttg cagggtgaaa
     6721 catgccagca tggctgagcc taagacagtg tactgggata gggacatggg aggaggcgga
     6781 tccggcggcg gcggaagcgg cggaggcggc tccggcggcg gaggaagcag cccacactcg
     6841 ctgcggtatt tcaccaccgc cgtgtcccgg cccggcctcg gggagccccg gttcatcatt
     6901 gtcggctacg tggacgacac gcagttcgtg cgcttcgaca gcgacgcgga aaatccgagg
     6961 atggagcctc gggcgcggtg gattgagcag gaggggccgg agtattggga gcgggagact
     7021 tggaaagcca gggacatggg gaggaacttc agagtaaacc tgaggaccct gctcggctac
     7081 tacaatcaga gtaacgacga atctcacacg ctgcagtgga tgtacggctg cgacgtgggg
     7141 cccgatgggc gcctgctccg cgggtattgt caggaggcct acgatggcca ggattacatc
     7201 tccctgaacg aggacctgcg ttcctggacc gcgaatgaca tagcctcaca gatctctaag
     7261 cacaagtcag aggcagtcga tgaggcccac caacagaggg catacctgca aggtccttgc
     7321 gtggagtggc tccatagata cctacggctg ggaaatgaga cactgcagcg ctcagaccct
     7381 ccaaaggcac atgtgaccca tcaccctaga tctgaagatg aagtcaccct gaggtgctgg
     7441 gccctgggct tctaccctgc tgacatcacc ctgacctggc agttgaatgg ggaggagctg
     7501 acccaggaca tggagcttgt ggagaccagg cctgcagggg atggaacctt ccagaagtgg
     7561 gcagctgtcg tggtgcctct tgggaaggag cagtattaca catgccatgt gtaccatgag
     7621 gggctgcctg agcccctcac cctgagatgg gagcctcctc catccactgt ctccaacatg
     7681 gtaatcatag ctgttctggt tgtccttgga gctgtgatca tccttggagc tgtggtggct
     7741 tttgtgatga agaggaggag acacataggt gtaaaaggat gctatgctca tgttctaggc
     7801 agcaagagct tccagacctc tgactggcct cagaaggcag gcagcggagt gaagcagacc
     7861 ctcaacttcg acctgctgaa gctggccgga gacgtggaga gcaatcctgg acccatgatc
     7921 gagcaggacg gactccatgc tggaagccct gccgcttggg tcgagaggct ctttggatac
     7981 gactgggctc agcagacaat cggctgcagc gatgccgccg tgtttagact cagcgcccag
     8041 ggcagacctg tgctgtttgt gaagaccgac ctgagcggag ctctcaatga gctccaagat
     8101 gaagccgcca gactgagctg gctcgccacc acaggcgtgc cttgtgctgc cgtgctggac
     8161 gtcgtcacag aggccggaag ggattggctg ctcctgggcg aagtccccgg acaggatctg
     8221 ctgagctccc atctggcccc tgccgaaaag gtctccatta tggccgatgc catgaggagg
     8281 ctgcacaccc tggatcccgc tacctgtccc tttgaccatc aggccaagca caggattgag
     8341 agggccagaa caagaatgga ggccggcctg gtcgaccagg atgatctgga cgaggaacac
     8401 cagggcctcg ctcctgccga gctctttgcc agactcaagg ccaggatgcc tgacggcgaa
     8461 gatctggtgg tgacccatgg agatgcctgt ctccccaaca tcatggtgga aaacggcagg
     8521 ttctccggat tcattgactg cggcaggctc ggcgtggccg acaggtatca ggacatcgcc
     8581 ctggctacca gagatatcgc tgaggagctg ggaggagagt gggccgatag atttctcgtg
     8641 ctctatggca ttgccgctcc cgacagccag agaattgcct tctacagact gctcgatgag
     8701 tttttttgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     8761 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     8821 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     8881 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     8941 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     9001 ccccacgcgc cctgtagcgg cgcatctagc ggccgatcag gcccgaccta ctctcttccg
     9061 cattggagtc gctttaactg gccctggctt tggcagcctg tgctgaccca tgcagtcctc
     9121 cttaccatcc ctccctcgac ttcccctctt ccgatgttga gcccctccag ccggtcctgg
     9181 actttgtctc cttccctgcc ctgccctctc ctgaacctga gccagctccc atagctcagt
     9241 ctggtctatc tgcctggccc tggccattgt cactttgcgc tgccctcctc tcgcccccga
     9301 gtgcccttgc tgtgccgccg gaactctgcc ctctaacgct gccgtctctc tcctgagtcc
     9361 ggaccacttt gagctctact ggcttctgcg ccgcctctgg cccactgttt ccccttccca
     9421 ggcaggtcct gctttctctg acctgcattc tctcccctgg gcctgtgccg ctttctgtct
     9481 gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc cgcctccttc
     9541 aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc tgcccaagga
     9601 tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc tgagcggatc
     9661 ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc catgccgtct
     9721 tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc tcgtttctta
     9781 ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc
     9841 atcaggccaa attggccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt
     9901 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9961 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    10021 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    10081 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10681 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10741 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10921 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10981 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    11041 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    11101 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    11161 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    11221 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11281 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11341 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    11401 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11461 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11521 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11581 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11641 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    11701 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    11761 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    11821 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11881 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11941 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    12001 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
