LOCUS       sequence-408956-       10607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1746
FEATURES             Location/Qualifiers
     source          1..10607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2517..2537
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2666..2783
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2921..4099
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3152..4090
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4047..4067
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4110..4119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6464..7052
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6517..6537)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6935..6946)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7124..7357
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7435..7556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7472..7491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7526..7545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7596..7731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7751..7770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7752..7770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7780..7797)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7780..7796)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7789..7811)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7938..8393
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8025..8044)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8235..8256
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8419..8523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8524..8592,8593..9384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8742..8761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9555..10143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10044..10063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10297..10314
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10431..10452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10467..10472,10473..10490,10491..10497)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10505..10521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10510..10532
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10529..10545
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10529..10545
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10564..10584
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10566..10584
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacn sacgccggag agcgtcgaag cgggggcggt
     2281 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     2341 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     2401 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     2461 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     2521 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     2581 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     2641 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2701 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2761 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     2821 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     2881 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
     2941 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     3001 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     3061 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     3121 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     3181 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     3241 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     3301 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     3361 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     3421 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     3481 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     3541 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     3601 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     3661 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3721 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3781 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3841 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3901 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3961 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     4021 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     4081 tccatttcag gtgtcgtgag gctagcaccg ccaccatggc tttttctcag ggtctattga
     4141 cattcaggga tgtggccata gaattctctc aggaggagtg gaaatgcctg gaccctgctc
     4201 agaggactct atacagggac gtgatgctgg agaattatag gaacctggtc tccctggata
     4261 tctcttccaa atgcacgatg aaggagttct tgtcaacagc gcaaggcaac agagaagtgt
     4321 tccatgcagg gacattgcaa atacatgaaa gtcatcacaa tggagatttt tgctaccagg
     4381 atgttgataa agatattcat gactatgaat ttcaatggca agaagatgaa agaaatggcc
     4441 atgaagcacc catgacaaaa atcaaaaagt tgacaggtat tacagaacga tatgatcaaa
     4501 gtcatgctag aaacaagcct attaaagatc agcttggatc aagctttcat tcgcatctgc
     4561 ctgaaatgca catatttcag accgaagaga aaattgataa tcaagttgtg aagtctgtcc
     4621 acgatgcttc cttggtttca acagcccaaa gaatttcttg taggcccaaa acccatatat
     4681 ctaataacca tgggaataat ttctggaatt cttcattact cacacaaaaa caggaagtac
     4741 acatgagaga aaaatctttc caatgtaatg agagtggcaa agcctttaat tacagctcac
     4801 tcttaaggaa acatcagata atccatttag cagacaaata taaatgtgat gtatgtggca
     4861 agctctttaa tcagaagcga aacctagcat gccatcgtag atgtcacact ggtgagaatc
     4921 cttacaagtg taatgagtgt ggcaagacct tcagtcagac gtcatccctt acatgccatc
     4981 gtagacttca tactggagag aaaccttaca aatgtgaaga atgtgacaaa gctttccatt
     5041 tcaaatcaat acttgaaaga cataggataa ttcatactga agagaaacca tataagtgta
     5101 atgagtgtgg caagaccttt aggcagaagt caatccttac acgccatcat cgacttcata
     5161 ctggagagaa accttacaag tgtaatgagt gtggcaagac ctttagtcac aagtcatccc
     5221 ttacatgcca tcatagactt catactggag agaaacccta caaatgtaat gagtgtggca
     5281 agacctttag tcacaagtca tcccttacat gccatcgtag acttcatact ggagagaaac
     5341 cttacaaatg tgaagaatgt gacaaagctt acagtttcag atcaaatttt gaaatacatc
     5401 ggaaaattca tactgaagac aatgcttaca agtgtaatga gtgtggaaag acctttagcc
     5461 ggacatcatc ccttacatgc catcgtagac gtcatactgg agagcaacct tacaaatgtg
     5521 aagaatgtga caaagctttc cgtttcaaat caaaccttga aagacatagg agaattcata
     5581 ctggagagaa accatacaag tgtaatgagt gtggcaagac ctttagtcgg aagtcatacc
     5641 tcacatgcca tcatagactt catactggag agaaagctta caagtgtaat gagtgcggca
     5701 agacctttag ttggaagtca tcccttacct gccatcgtag acttcattct ggagagaaac
     5761 cttacaagtg taaggagtgt ggcaagacct tcaatcagca gttaaccctt aaacgccatc
     5821 gtagacttca tagtggagag aacccttaca aatgtgaaga tagtgacaaa gcttacagtt
     5881 tcaaatcaaa ccttgaaata catcagaaaa ttcatactga agagaatcct tacaagtgta
     5941 atgagtgtgg caagaccttc agtcggacgt catcccttac atgccatcgt agacttcata
     6001 ccggagagaa accttacaaa tgtgaagaat gtgacaaagc tttccgtgtg aaatcaaacc
     6061 ttgaaggaca taggagaatt catactggag agaaacctta caagtgtaat gagtgtggca
     6121 agacctttag tcggaagtca tattttatat gccatcatag acttcatact ggagagaaac
     6181 cttataagtg taatgagtgt ggcaagaact ttagtcagaa gtcatccctt atatgccacc
     6241 atagacttca tactggagag aaaccttaca agtgtaatga gtgtggcaag acctttagtc
     6301 agaagtcaaa ccttacatgc catcgtagac ttcatactgg agaaaaacaa gtgtagtaat
     6361 gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     6421 acgacggcgc gggctcgagt gtgaaggaac gcgttaagtc gacaatcaac ctctggatta
     6481 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6541 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6601 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6661 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6721 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6781 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6841 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6901 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6961 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7021 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     7081 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7141 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7201 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7261 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7321 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     7381 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7501 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7561 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7621 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7681 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     7741 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     7801 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7861 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7921 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7981 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     8041 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     8101 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8161 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     8221 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8281 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     8341 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     8401 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8461 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8521 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8581 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8641 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8701 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8761 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8821 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8881 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8941 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9001 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9061 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9121 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9181 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9241 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9301 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9361 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9421 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9481 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9541 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9601 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9661 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9721 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9781 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9841 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9901 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9961 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10021 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10081 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10141 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10201 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    10261 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    10321 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    10381 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    10441 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    10501 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    10561 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagc
//
