LOCUS       sequence-408979-        9940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0223
FEATURES             Location/Qualifiers
     source          1..9940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2878..3466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2931..2951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3349..3360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3538..3771
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3849..3970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3886..3905)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3940..3959
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4010..4145
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4165..4184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4166..4184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4194..4211)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4194..4210)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4203..4225)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4352..4807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4439..4458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4649..4670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4833..4937
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4938..5006,5007..5798)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5156..5175)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5969..6557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6458..6477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6711..6728
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6845..6866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6881..6886,6887..6904,6905..6911)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6919..6935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6924..6946
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6943..6959
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6943..6959
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6978..6998
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6980..6998
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7026..7252
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7253..7433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7480..7605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8098..8331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8516..8560
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8791..9001
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8813..9009
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8860..8995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9041..9643
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9041..9060)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9537..9557
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9686..9803
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 taaaagacca ggaccccctg gaagccgaga aatgggactg ttgacattca gagacatagc
     1261 tatagaattc tctctggagg aatggcaatg cctggattgt gctcagcgga atttatatag
     1321 agatgtgatg ttagagaact acagaaacct ggtctccctg ggtattgctg tctctaagcc
     1381 agacttgatc acctgtctgg agcaaaataa agagtcccag aatataaaga gaaatgagat
     1441 ggtagccaaa cacccagtta cgcgttccca tttcacccaa gaccttcagc cagagcaggg
     1501 catcaaagat tcactccaaa aagtaatacc aagaacatat ggaaaatgtg gacatgagaa
     1561 attacaattt aaaaaatgct gtaaaagtgt gggtgaatat gaggtgcaca agggaggtta
     1621 tagtgaagtt aaccaatgtt tgtcaactac ccaaaacaaa atatttcaga ctcataaata
     1681 tgtcaaagtc tttggtaaat tttcaaattc caatagagat aaaacaagat atactggaaa
     1741 taaacatttc aaatgtaaca aatatggcaa atcattttgc atgctttcac acctaaatca
     1801 acatcaggta attcatacta gggagaagtc ctacaaatgc aaagaatgtg gcaaatcctt
     1861 taactgctcc tcaaaccata ctacacataa aataattcat actggagaga aaccatatag
     1921 atgtgaggaa tgtggcaaag cctttagctg gtctgcaaac cttactagac ataagagaac
     1981 tcatactgga gagaaaccct acacgtgtga agaatgtggc caagccttta ggcgctcctc
     2041 agcacttact aaccacaaga gaattcatac tggagagaga ccctacaaat gtgaagaatg
     2101 tggcaaagcc tttagcgtat cctcaaccct cactgaccac aagagaattc atactggaga
     2161 gaaaccctgc aggtgtgagg aatgtggcaa agcctttagc tggtcctcaa accttactag
     2221 acataagaga attcatacta gagagaaacc ctatgcctgt gaagaatgtg gccaagcctt
     2281 tagcttatcc tcgaacctta tgagacatag gagaattcat actggagaga aaccctacac
     2341 atgtgaagaa tgtggccaag actttaggcg ctcctcagca cttactatcc acaagagaat
     2401 tcatactgga gagagaccct acaaatgtga agagtgtggc aaagtcttta gcttatcctc
     2461 aaccctcact gaccacaaga gaattcatac tggagagaga ccctacaaat gtgaagaatg
     2521 tggcaaagcc tttagcttat cctcaaccct cactgaccac aagagaattc atactggaga
     2581 gagaccctac acatgtgaag aatgtggcaa agcctttaat tgctcctcaa cccttatgca
     2641 acataagaga attcatactg gagagaaacc ctacaaatgt gaagaatgtg agcaagcttt
     2701 taagtggcat tcaagtcttg ctaaacataa gataattcac actggagaga aaccctacaa
     2761 atgtgaatag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     2821 atcttgtgga aaggacgata ctcacgccag gtcagggagc tcacgcgtta agtcgacaat
     2881 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2941 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3001 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3061 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3121 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3181 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3241 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3301 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3361 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3421 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3481 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3541 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3601 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3661 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3721 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3781 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3841 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3961 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4021 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4081 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4141 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4201 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4261 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4321 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4381 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4441 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4501 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4561 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4621 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4681 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4741 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4801 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4861 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4921 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4981 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5041 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5101 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5161 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5221 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5281 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5341 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5401 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5461 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5521 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5581 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5641 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5701 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5761 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5821 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5881 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5941 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6001 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6061 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6601 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6661 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6721 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6781 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6841 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6901 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6961 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7021 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7081 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7141 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7201 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7261 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7321 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7381 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7441 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7501 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7561 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7621 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7681 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7741 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7801 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7861 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7921 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     7981 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8041 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8101 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8161 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8221 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8281 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8341 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8401 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8461 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8521 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8581 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8641 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8701 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     8761 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     8821 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8881 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8941 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9001 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9061 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9121 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9181 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9241 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9301 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9361 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9421 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9481 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9541 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9601 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     9661 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9721 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9781 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9841 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     9901 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
