LOCUS       sequence-408982-        9973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0737
FEATURES             Location/Qualifiers
     source          1..9973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2911..3499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2964..2984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3382..3393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3571..3804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3882..4003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3919..3938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3973..3992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4043..4178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4198..4217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4199..4217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4227..4244)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4227..4243)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4236..4258)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4385..4840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4472..4491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4682..4703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4866..4970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4971..5039,5040..5831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5189..5208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6002..6590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6491..6510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6744..6761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6878..6899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6914..6919,6920..6937,6938..6944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6952..6968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6957..6979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6976..6992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6976..6992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7011..7031
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7013..7031
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7059..7285
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7286..7466
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7513..7638
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8131..8364
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8549..8593
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8824..9034
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8846..9042
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8893..9028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9074..9676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9074..9093)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9570..9590
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9719..9836
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgct
     1201 gtgtgatgaa aaagcccaga agagaaggaa gaggaaagca aaggagtcag ggatggctct
     1261 tcctcaggga cacttaacat tcagggacgt ggccatagaa ttctctcagg cggagtggaa
     1321 atgcctggac cctgcacaga gggctttata caaggatgtg atgttggaga actacaggaa
     1381 cctggtctcc ctgggaatct ctcttcctga cctgaatatt aactccatgt tggagcaaag
     1441 gagggagccc tggtctggtg agagtgaagt gaaaatagca aaaaattcag atgggaggga
     1501 gtgcatcaaa ggtgtgaaca cagggagcag ctatgcattg ggaagcaatg cagaagacaa
     1561 accaattaaa aaacaacttg gagtatcctt tcacttacat ctgtctgaac tggagctatt
     1621 tccagatgaa agggtaataa atggatgtaa tcaagttgaa aactttatca accacagttc
     1681 ctctgtttcc tgtcttcaag aaatgtcttc cagtgtcaaa acccccattt ttaataggaa
     1741 tgattttgat gattcttcat ttctcccaca agaacagaaa gtacacctta gagaaaaacc
     1801 ttatgaatgt aatgagcata gcaaagtctt tagagtatct tccagcctta ctaaacatca
     1861 agtaatccat actgtagaga aaccttacaa atgtaattca tgcggcaagg tctttagtcg
     1921 caattcacac cttgcagaac attgtagaat tcatactgga gagaaacctt acaaatgtaa
     1981 tgtctgtggc aaggttttta gttacaattc aaactttgca cgacatcaaa gaattcatac
     2041 cagagagaag ccgtatgaat gtaatgaatg tggtaaagtc ttcagtaata attcttacct
     2101 tgcacgacat caaagaattc atgctgaaga gaaaccttac aaatgtaatg aatgtggtaa
     2161 aggcttcagt cataagtcat ctctagcaaa tcattggaga atttatactg gagagaagcc
     2221 ttacaaatgt gatgaatgtg gcaaggcctt ctataggatt gcgctccttg tacgacatca
     2281 gaaaattcat actggagaga aaccttacaa atgtaatgaa tgtggaaagg tctttattca
     2341 aaattcgcac ctagcacaac attggagaat tcatacagga gagaaacctt acaaatgtaa
     2401 tgaatgtgga aaagtattta atcaactttc aaatcttgca cgacatcgaa gaattcatac
     2461 tggagagaag ccttacaaat gtaatgaatg tggtaaagca tttagtgagt attcaggcct
     2521 ttcagcccat cttgtaatcc acactggaga gaagccttac aaatgtagtg aatgtggcaa
     2581 ggcattcaga cacaagttat cactaaccaa tcatcagaga atccatactg gagaaagacc
     2641 ttacaaatgt aatgaatgtg gcaaggtctt caatcgaatt gcacaccttg cacgacatcg
     2701 gaaaattcat actggagaga aaccttacaa atgtaatgag tgtggcaagg cctttagtcg
     2761 catttcatac ctagcacaac attggacaat tcatatggga tagtaatgaa ctagtccgta
     2821 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg ataccagtaa
     2881 tctggacgta tcgttacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     2941 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3001 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3061 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3121 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3181 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3241 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3301 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3361 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3421 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3481 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3541 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3601 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3781 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     3841 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     4021 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4141 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4201 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4261 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4321 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4381 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4441 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4501 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4561 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4621 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4681 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4741 ttataaggaa ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4801 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5521 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5581 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5821 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5881 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6901 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6961 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     7021 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     7081 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7141 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7201 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7261 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7321 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7381 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7441 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7501 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7561 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7621 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7681 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7741 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7801 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7861 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7921 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7981 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8041 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8101 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8161 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8221 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8281 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8341 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8401 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8461 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8521 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8581 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8641 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8701 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8761 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8821 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     8881 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8941 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9001 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     9061 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     9121 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     9181 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     9241 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     9301 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     9361 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     9421 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     9481 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9541 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9601 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9661 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9721 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9781 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9841 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
     9901 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     9961 aggagtggga att
//
