LOCUS       sequence-408990-        9269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to constitutively express TAZ/WWTR1 under control 
            of the human PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, 
            Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, 
            Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek
            D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, 
            Goodrich DW, Bremner R
  TITLE     Binary pan-cancer classes with distinct vulnerabilities defined by 
            pro- or anti-cancer YAP/TEAD activity.
  JOURNAL   Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016.
  PUBMED    34270926
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016."
COMMENT     Sequence Label: PGKp-GFP-TAZ
FEATURES             Location/Qualifiers
     source          1..9269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..72
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             73..672
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(73..92)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     569..589
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             800..1033
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1105..1226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1142..1161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1196..1215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1266..1401
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1421..1440)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1422..1440)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1450..1467)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1450..1466)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1459..1481)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1608..2063
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1695..1714)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1905..1926
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2089..2193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2194..2262,2263..3054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2412..2431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3225..3813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3714..3733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3967..3984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4101..4122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4137..4142,4143..4160,4161..4167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4175..4191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4180..4202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4199..4215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4199..4215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4234..4254
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4236..4254
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4282..4508
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4509..4689
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4736..4861
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5354..5587
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5772..5816
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6029..6146
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6195..6705
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6624..6642
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6725..6754
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(7975..8549,8550..8552,8553..8561)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8142..8159)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8369..8388
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8562..8564,8565..8567,8568..9269,1..9)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8562..8564,8565..8567,8568..9269,1..9)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8607..8628)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8868..8887)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9215..9236
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctgtacaagg gcagcggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
       61 aatcctggac ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
      121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
      181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
      241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
      301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
      361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
      421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
      481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
      541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
      601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
      661 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
      721 catgcatcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
      781 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
      841 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1021 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     1081 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     1141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1201 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     1261 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1321 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     1381 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     1441 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1501 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1561 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     1621 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1681 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1741 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1801 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1861 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1921 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1981 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2041 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     2101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2161 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2221 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2281 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2341 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2401 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2461 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2521 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2581 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2641 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2701 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2761 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2821 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2881 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2941 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3001 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3061 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3121 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3181 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3241 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3301 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3361 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3421 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3481 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3541 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3601 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3661 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3721 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3781 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3841 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3901 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3961 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4021 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4081 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4141 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4201 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     4261 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     4321 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     4381 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     4441 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     4501 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4561 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4621 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4681 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     4741 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     4801 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     4861 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     4921 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     4981 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     5041 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     5101 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     5161 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     5221 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     5281 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     5341 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     5401 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     5461 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     5521 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     5581 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     5641 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     5701 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     5761 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     5821 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     5881 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     5941 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggacaa ttctcgacct
     6001 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     6061 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     6121 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     6181 ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     6241 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     6301 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     6361 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     6421 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     6481 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     6541 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     6601 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     6661 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag
     6721 catggaacaa aaactcatct cagaagagga tctgaccggt aatccggcct cggcgccccc
     6781 tccgctcccg ccgcctgggc agcaagtgat ccacgtcacg caggacctag acacagacct
     6841 cgaagccctc ttcaactctg tcatgaatcc gaagcctagc tcgtggcgga agaagatcct
     6901 gccggagtct ttctttaagg agcctgattc gggctcgcac tcgcgccagt ccagcaccga
     6961 ctcgtcgggc ggccacccgg ggcctcgact ggctgggggt gcccagcatg tccgctcgca
     7021 ctcgtcgccc gcgtccctgc agctgggcac cggcgcgggt gctgcgggta gccccgcgca
     7081 gcagcacgcg cacctccgcc agcagtccta cgacgtgacc gacgagctgc cactgccccc
     7141 gggctgggag atgaccttca cggccactgg ccagaggtac ttcctcaatc acatagaaaa
     7201 aatcaccaca tggcaagacc ctaggaaggc gatgaatcag cctctgaatc atatgaacct
     7261 ccaccctgcc gtcagttcca caccagtgcc tcagaggtcc atggcagtat cccagccaaa
     7321 tctcgtgatg aatcaccaac accagcagca gatggccccc agtaccctga gccagcagaa
     7381 ccaccccact cagaacccac ccgcagggct catgagtatg cccaatgcgc tgaccactca
     7441 gcagcagcag cagcagaaac tgcggcttca gagaatccag atggagagag aaaggattcg
     7501 aatgcgccaa gaggagctca tgaggcagga agctgccctc tgtcgacagc tccccatgga
     7561 agctgagact cttgccccag ttcaggctgc tgtcaaccca cccacgatga ccccagacat
     7621 gagatccatc actaataata gctcagatcc tttcctcaat ggagggccat atcattcgag
     7681 ggagcagagc actgacagtg gcctggggtt agggtgctac agtgtcccca caactccgga
     7741 ggacttcctc agcaatgtgg atgagatgga tacaggagaa aacgcaggac aaacacccat
     7801 gaacatcaat ccccaacaga cccgtttccc tgatttcctt gactgtcttc caggaacaaa
     7861 cgttgactta ggaactttgg aatctgaaga cctgatcccc ctcttcaatg atgtagagtc
     7921 tgctctgaac aaaagtgagc cctttctaac ctggctgtaa gaattccgcc cccccccctc
     7981 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     8041 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     8101 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     8161 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     8221 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     8281 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     8341 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     8401 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     8461 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     8521 ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg
     8581 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     8641 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     8701 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     8761 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     8821 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     8881 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     8941 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     9001 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     9061 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     9121 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     9181 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     9241 ccgccgggat cactctcggc atggacgag
//
