LOCUS       sequence-409030-       12398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, 
            mouse CD47, and mouse CD55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 12398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb
            SCT-T2A-mCD...
FEATURES             Location/Qualifiers
     source          1..12398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..63
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(101..123)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     223..242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     436..458
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     450..467
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     451..467
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1904..3082
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2135..3073
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3030..3050
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3099..3118
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3099..3117
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4353..4409
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5922..5975
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6960..7019
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             8199..8264
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(9079..9096)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9085..9309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10249..10265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10249..10265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10262..10284)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10273..10289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10297..10303,10304..10321,10322..10327))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10342..10363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10480..10497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10651..11239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10731..10750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11410..12201,12202..12270))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12033..12052
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12271..12375)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
       61 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      121 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      181 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
      241 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      301 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      361 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      421 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgccgtga
      481 cgtcagcacg ccgggcgggg accgggagat ccttggggcg gtggggggcc agcggcagtt
      541 cccggcggcc cccggggcgg gcgggcgggc gggtggtggc ggcggttggg gctcggcgct
      601 cgctcgctcg ctgggcgggc gggcggtgcg atgtccggag aggatggccc ggcggctggc
      661 ccgggggcgg cggcggcggc tgcccgggag cggcgacggg agcagctgcg gcagtggggg
      721 gcgcgggcgg gcgccgagcc tggccccgga gagcgccgcg cccgcaccgt ccgcttcgag
      781 cgcgccgccg agttcctggc ggcctgtgcg ggcggcgacc tggacgaggc gcgtctgatg
      841 ctgcgcgccg ccgaccctgg ccccggcgcc gagctcgacc ccgccgcgcc gccgcccgcc
      901 cgcgccgtgc tggactccac caacgccgac ggtatcagcg ccctgcacca ggtcagcgcc
      961 ccccgcccgg cgtctcccgg ggccaggtcc accctctgct gcgccacctg gggcatcctc
     1021 cttccccgtt gccagtctcg atccgccccg tcgttcctgg ccctgggctt tgccacccta
     1081 tgctgacacc ccgtcccagt cccccttacc attccccttc gaccacccca cttccgaatt
     1141 ggagccgctt caactggccc tgggcttagc cactctgtgc tgaccactct gccccaggcc
     1201 tccttaccat tccccttaaa caccggtggg gaggtggtcc ctgctgttac gccaatgata
     1261 acccccgcca gaaaaatctt agtagccttc cctttttgtt ttccgtgccc caactcggcg
     1321 gattgactcg gccccttccg gaaacacccg aatcaacttc tagtcaaatt attgttcacg
     1381 ccgcaatgac ccacccctgg cccgcgtctg tggaactgac ccctggtgta caggagagtt
     1441 cgctgctgaa agtggtccca aaggggtact agtttttaag ctcccaactc cccctccccc
     1501 agcgtctgga ggattccaca ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt
     1561 tttggcgcca gccgctgagg ctgccaagca gaaaagccac cgctgaggag actccggtca
     1621 ctgtcctcgc cccgcctccc ccttccctcc ccttggggac caccgggcgc cacgccgcga
     1681 acgttaagtg ccgcggtcgt cggcgcctcc gccctccccc ttgggcccca attcccagcg
     1741 ggcgcggcgc gcggcccctc cccccgccgg gcgcgcgccc gctgccccgc ccttcgtggc
     1801 cgcccggcgt gggcggtgcc acccctcccc ccggcggccc cgcgcgcagc tcccggctcc
     1861 ctcccccttc ggatgtggct tgagctgtag gcgcggaggg ccgggctccg gtgcccgtca
     1921 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1981 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2041 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2101 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2161 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2221 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2281 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2341 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2401 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2461 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2521 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2581 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2641 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2701 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2761 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2821 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2881 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2941 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3001 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3061 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     3121 acccaagctg gctaggtaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     3181 attctgcaga tatccagcac agtggcggcc gcaccatggc cagctatcct tgtcaccagc
     3241 atgcctccgc ttttgaccag gccgctagat ccagaggcca ttccaacagg aggacagccc
     3301 tcagacccag gaggcagcaa gaggccacag aggtcaggct ggagcagaaa atgcccaccc
     3361 tcctgagggt gtacattgat ggccctcacg gcatgggaaa gaccacaaca acccaactgc
     3421 tggtcgccct gggctccagg gatgatattg tgtacgtgcc cgaacccatg acctactgga
     3481 gggtgctggg cgcctccgag accatcgcta atatctacac cacccaacac aggctcgatc
     3541 agggcgagat ttccgctggc gatgctgctg tggtcatgac ctccgcccag atcaccatgg
     3601 gcatgcccta cgccgtgaca gatgccgtcc tggctcctca tatcggagga gaagccggca
     3661 gctcccatgc tcctcctccc gccctcacac tgatctttga cagacacccc atcgccgccc
     3721 tcctgtgtta ccctgccgct agatacctga tgggaagcat gacaccccag gccgtgctcg
     3781 ctttcgtggc tctgattccc cctaccctgc ccggcacaaa catcgtcctg ggcgctctgc
     3841 ccgaagatag gcacatcgat aggctcgcca aaaggcaaag gcccggagaa agactggacc
     3901 tcgctatgct cgctgctatt aggagagtgt acggcctcct cgccaacacc gtgagatatc
     3961 tccagggcgg cggcagctgg agagaggatt ggggccagct gtccggcgct gccgtgcctc
     4021 ctcagggagc cgaacctcaa agcaatgctg gccctagacc ccacatcggc gacaccctct
     4081 tcaccctgtt tagggccccc gaactgctcg cccccaacgg cgacctctac aacgtctttg
     4141 cctgggccct ggacgtgctg gccaaaaggc tgaggcctat gcacgtgttc atcctggact
     4201 atgaccagtc ccccgccggc tgcagggatg ccctcctcca gctgaccagc ggaatggtgc
     4261 agacccacgt gacaaccccc ggcagcatcc ccaccatttg cgacctggcc aggacattcg
     4321 ccagagagat gggcgaagct aatggatccg gagccaccaa cttcagcctg ctgaagcaag
     4381 ccggcgacgt ggaggaaaac cccggcccca tggccaggtc cgtgacactc gtcttcctgg
     4441 tgctggtgtc cctgaccgga ctgtatgcta tggtcatgag cttcgtgaat ctgggatgcg
     4501 gcgccagcgg aggcggaggc tccggcggcg gcggcagcat ccaaaagaca cctcagatcc
     4561 aggtgtattc caggcaccct cctgaaaatg gcaaacctaa tattctcaac tgttacgtca
     4621 cacagtttca ccccccccac attgaaatcc agatgctgaa gaacggcaag aaaatcccca
     4681 aggtcgaaat gagcgacatg agcttcagca aggactggtc cttttacatc ctggctcaca
     4741 ccgagtttac ccctaccgag accgatacct acgcctgcag agtgaagcac gccagcatgg
     4801 ccgagcctaa gacagtctac tgggacagag acatgggagg cggcggcagc ggcggcggcg
     4861 gcagcggagg aggaggctcc ggaggaggcg gcagcggacc ccactccctg aggtactttg
     4921 tcacagccgt cagcagaccc ggactcggag agcctaggta tatggaagtg ggatacgtgg
     4981 acgacaccga gttcgtgagg tttgactccg acgctgagaa ccctagatac gaacccaggg
     5041 ccaggtggat ggagcaagag ggccctgagt attgggaaag ggaaacccag aaggccaaag
     5101 gcaacgaaca gagcttcagg gtcgacctca gaacactgct gggctgctat aaccagagca
     5161 agggaggcag ccataccatc caggtgatca gcggctgcga ggtcggatcc gacggcagac
     5221 tgctgagggg atatcagcag tacgcttacg acggctgcga ttatattgcc ctgaacgagg
     5281 atctgaaaac ctggaccgct gctgatatgg ctgccctgat caccaagcat aagtgggaac
     5341 aggctggcga ggctgagagg ctgagagctt acctcgaagg cacatgtgtc gagtggctga
     5401 ggaggtacct caagaacggc aatgccacac tcctgagaac cgatagccct aaggctcacg
     5461 tcacacatca tagcaggccc gaggacaagg tcaccctgag atgttgggcc ctgggatttt
     5521 accccgccga tatcaccctg acctggcaac tgaacggaga ggagctgatc caggatatgg
     5581 aactggtgga aaccagacct gctggcgacg gcaccttcca aaagtgggcc agcgtggtcg
     5641 tccctctggg aaaagagcaa tactatacct gccacgtcta ccatcagggc ctgcctgagc
     5701 ctctcaccct gagatgggag cctcccccca gcaccgtcag caatatggcc accgtggctg
     5761 tgctcgtggt gctgggagcc gccattgtga caggcgccgt ggtggccttc gtcatgaaga
     5821 tgaggaggag gaacaccgga ggcaaaggcg gagactatgc cctcgctccc ggcagccaga
     5881 catccgacct cagcctgccc gactgtaagg ctggctccgg cgaaggcaga ggaagcctcc
     5941 tgacctgtgg agatgtcgag gaaaatcccg gccccatgtg gcccctggct gctgccctcc
     6001 tgctgggaag ctgttgctgt ggctccgctc agctgctgtt tagcaacgtc aactccatcg
     6061 agtttacaag ctgtaacgag accgtggtca tcccctgtat cgtgaggaac gtggaggccc
     6121 agtccaccga agagatgttc gtcaagtgga aactgaacaa aagctatatc ttcatttacg
     6181 acggaaataa aaactccacc accacagacc agaactttac cagcgccaaa atctccgtct
     6241 ccgatctcat caatggcatc gccagcctga agatggacaa gagggatgct atggtcggca
     6301 attacacctg cgaggtgaca gaactcagca gagagggcaa gaccgtgatc gagctcaaaa
     6361 ataggaccgc ctttaataca gaccaaggct ccgcttgcag ctatgaagag gaaaaaggag
     6421 gatgcaagct cgtcagctgg ttttccccca atgagaagat tctcattgtg atcttcccca
     6481 tcctggccat tctcctcttt tggggcaagt tcggaatcct caccctcaag tacaagtcca
     6541 gccacaccaa caagagaatt atcctgctcc tggtggccgg cctggtcctc accgtgatcg
     6601 tggtggtcgg cgccattctg ctcattcccg gagagaagcc tgtcaaaaac gcctccggcc
     6661 tcggactcat tgtgatcagc accggaatcc tgatcctcct gcagtacaac gtgttcatga
     6721 cagctttcgg aatgacctcc ttcaccatcg ctatcctgat tacccaggtg ctgggatatg
     6781 tgctggccct cgtgggactg tgtctgtgca tcatggcttg cgaacccgtc catggccctc
     6841 tcctgatctc cggcctgggc atcatcgccc tcgctgagct gctcggactg gtgtacatga
     6901 aattcgtggc ctccaaccaa agaaccatcc agcctcccag gaacagaggc ggatccggcc
     6961 agtgcacaaa ctatgccctg ctcaagctgg ccggcgatgt ggaaagcaat cccggcccta
     7021 tgatcagggg aagggcccct aggacaaggc ctagccctcc tccccctctc ctccctctcc
     7081 tgagcctgag cctgctgctg ctgtccccta ccgtgagagg cgactgcggc cccccccccg
     7141 acattcctaa cgccagacct attctgggca ggcatagcaa atttgccgaa caaagcaagg
     7201 tcgcctacag ctgcaacaac ggcttcaagc aggtccccga caaaagcaac attgtcgtgt
     7261 gcctcgagaa cggccaatgg tccagccatg aaacattctg cgaaaaaagc tgcgtggctc
     7321 ccgagaggct gtccttcgcc tccctgaaaa aggaatatct gaatatgaat ttcttccctg
     7381 tgggcaccat tgtggagtat gagtgtaggc ccggattcag aaagcagccc cctctgcccg
     7441 gaaaagctac atgtctggaa gatctggtgt ggagccccgt ggctcaattc tgcaaaaaga
     7501 agagctgtcc caatcccaag gacctcgata atggccacat caacattcct accggaatcc
     7561 tgttcggcag cgaaattaac tttagctgca atcccggata taggctcgtc ggcgtcagca
     7621 gcaccttctg ttccgtgacc ggaaacaccg tggactggga cgacgaattc cccgtctgta
     7681 ccgagattca ttgccctgag cctcctaaga ttaacaatgg catcatgaga ggcgaaagcg
     7741 acagctacac ctattcccag gtcgtcacat atagctgcga caagggattc atcctggtcg
     7801 gcaatgctag catctattgc accgtgagca aatccgatgt gggacaatgg agcagccccc
     7861 ctcccaggtg catcgaaaag agcaaagtgc ccaccaaaaa acccacaatc aacgtgccta
     7921 gcacaggcac accttccacc cctcaaaaac ctacaaccga gtccgtgccc aatcctggag
     7981 atcagcccac ccctcagaaa ccttccacag tcaaggtgag cgccacccag catgtccctg
     8041 tgaccaagac aacagtcaga catcctatca gaacctccac cgacaaagga gaacctaaca
     8101 ccggcggaga caggtacatt tacggacaca cctgtctgat cacactgaca gtgctccatg
     8161 tcatgctgtc cctgatcgga tacctcacag gctccggcgt gaaacaaacc ctgaacttcg
     8221 atctgctgaa actcgccgga gacgtcgagt ccaaccccgg ccctatgatc gagcaggacg
     8281 gactccatgc tggaagccct gccgcttggg tcgagaggct ctttggatac gactgggctc
     8341 agcagacaat cggctgcagc gatgccgccg tgtttagact cagcgcccag ggcagacctg
     8401 tgctgtttgt gaagaccgac ctgagcggag ctctcaatga gctccaagat gaagccgcca
     8461 gactgagctg gctcgccacc acaggcgtgc cttgtgctgc cgtgctggac gtcgtcacag
     8521 aggccggaag ggattggctg ctcctgggcg aagtccccgg acaggatctg ctgagctccc
     8581 atctggcccc tgccgaaaag gtctccatta tggccgatgc catgaggagg ctgcacaccc
     8641 tggatcccgc tacctgtccc tttgaccatc aggccaagca caggattgag agggccagaa
     8701 caagaatgga ggccggcctg gtcgaccagg atgatctgga cgaggaacac cagggcctcg
     8761 ctcctgccga gctctttgcc agactcaagg ccaggatgcc tgacggcgaa gatctggtgg
     8821 tgacccatgg agatgcctgt ctccccaaca tcatggtgga aaacggcagg ttctccggat
     8881 tcattgactg cggcaggctc ggcgtggccg acaggtatca ggacatcgcc ctggctacca
     8941 gagatatcgc tgaggagctg ggaggagagt gggccgatag atttctcgtg ctctatggca
     9001 ttgccgctcc cgacagccag agaattgcct tctacagact gctcgatgag tttttttgag
     9061 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     9121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     9181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     9241 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     9301 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     9361 cctgtagcgg cgcatctagc ggccgatcag gcccgaccta ctctcttccg cattggagtc
     9421 gctttaactg gccctggctt tggcagcctg tgctgaccca tgcagtcctc cttaccatcc
     9481 ctccctcgac ttcccctctt ccgatgttga gcccctccag ccggtcctgg actttgtctc
     9541 cttccctgcc ctgccctctc ctgaacctga gccagctccc atagctcagt ctggtctatc
     9601 tgcctggccc tggccattgt cactttgcgc tgccctcctc tcgcccccga gtgcccttgc
     9661 tgtgccgccg gaactctgcc ctctaacgct gccgtctctc tcctgagtcc ggaccacttt
     9721 gagctctact ggcttctgcg ccgcctctgg cccactgttt ccccttccca ggcaggtcct
     9781 gctttctctg acctgcattc tctcccctgg gcctgtgccg ctttctgtct gcagcttgtg
     9841 gcctgggtca cctctacggc tggcccagat ccttccctgc cgcctccttc aggttccgtc
     9901 ttcctccact ccctcttccc cttgctctct gctgtgttgc tgcccaagga tgctctttcc
     9961 ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc tgagcggatc ctccccgtgt
    10021 ctgggtcctc tccgggcatc tctcctccct cacccaaccc catgccgtct tcactcgctg
    10081 ggttcccttt tccttctcct tctggggcct gtgccatctc tcgtttctta ggatggcctt
    10141 ctccgacgga tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc atcaggccaa
    10201 attggccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt
    10261 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10321 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10381 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10441 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10501 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10561 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10621 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10681 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10741 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10801 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10861 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10921 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10981 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11041 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    11101 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11161 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11221 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11281 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11341 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11401 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11461 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11521 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11581 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11641 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11701 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11761 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11821 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11881 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11941 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12001 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12061 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12121 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12181 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12241 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12301 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12361 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
