LOCUS       sequence-409031-       11349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human AAVS1-targeting plasmid for expression of human CD46, HLA-G 
            SCT, CD47, and CD59.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E
            SCT-T2A-hCD...
FEATURES             Location/Qualifiers
     source          1..11349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..63
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(101..123)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     223..242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     436..458
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     450..467
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     451..467
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1904..3082
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2135..3073
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3030..3050
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3099..3118
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3099..3117
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3150..3470
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(3822..3833)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4347..4373
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4389..4445
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5955..6008
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7161..7220
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7224..8018
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7278..7297)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7888..7907
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8030..8047)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8036..8260
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9200..9216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9200..9216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9213..9235)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9224..9240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9248..9254,9255..9272,9273..9278))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9293..9314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9431..9448)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9602..10190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9682..9701)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10361..11152,11153..11221))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10984..11003
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11222..11326)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
       61 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      121 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      181 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
      241 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      301 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      361 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      421 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgccgtga
      481 cgtcagcacg ccgggcgggg accgggagat ccttggggcg gtggggggcc agcggcagtt
      541 cccggcggcc cccggggcgg gcgggcgggc gggtggtggc ggcggttggg gctcggcgct
      601 cgctcgctcg ctgggcgggc gggcggtgcg atgtccggag aggatggccc ggcggctggc
      661 ccgggggcgg cggcggcggc tgcccgggag cggcgacggg agcagctgcg gcagtggggg
      721 gcgcgggcgg gcgccgagcc tggccccgga gagcgccgcg cccgcaccgt ccgcttcgag
      781 cgcgccgccg agttcctggc ggcctgtgcg ggcggcgacc tggacgaggc gcgtctgatg
      841 ctgcgcgccg ccgaccctgg ccccggcgcc gagctcgacc ccgccgcgcc gccgcccgcc
      901 cgcgccgtgc tggactccac caacgccgac ggtatcagcg ccctgcacca ggtcagcgcc
      961 ccccgcccgg cgtctcccgg ggccaggtcc accctctgct gcgccacctg gggcatcctc
     1021 cttccccgtt gccagtctcg atccgccccg tcgttcctgg ccctgggctt tgccacccta
     1081 tgctgacacc ccgtcccagt cccccttacc attccccttc gaccacccca cttccgaatt
     1141 ggagccgctt caactggccc tgggcttagc cactctgtgc tgaccactct gccccaggcc
     1201 tccttaccat tccccttaaa caccggtggg gaggtggtcc ctgctgttac gccaatgata
     1261 acccccgcca gaaaaatctt agtagccttc cctttttgtt ttccgtgccc caactcggcg
     1321 gattgactcg gccccttccg gaaacacccg aatcaacttc tagtcaaatt attgttcacg
     1381 ccgcaatgac ccacccctgg cccgcgtctg tggaactgac ccctggtgta caggagagtt
     1441 cgctgctgaa agtggtccca aaggggtact agtttttaag ctcccaactc cccctccccc
     1501 agcgtctgga ggattccaca ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt
     1561 tttggcgcca gccgctgagg ctgccaagca gaaaagccac cgctgaggag actccggtca
     1621 ctgtcctcgc cccgcctccc ccttccctcc ccttggggac caccgggcgc cacgccgcga
     1681 acgttaagtg ccgcggtcgt cggcgcctcc gccctccccc ttgggcccca attcccagcg
     1741 ggcgcggcgc gcggcccctc cccccgccgg gcgcgcgccc gctgccccgc ccttcgtggc
     1801 cgcccggcgt gggcggtgcc acccctcccc ccggcggccc cgcgcgcagc tcccggctcc
     1861 ctcccccttc ggatgtggct tgagctgtag gcgcggaggg ccgggctccg gtgcccgtca
     1921 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1981 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2041 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2101 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2161 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2221 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2281 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2341 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2401 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2461 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2521 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2581 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2641 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2701 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2761 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2821 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2881 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2941 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3001 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3061 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     3121 acccaagctg gctaggtacc atgctcgagg gagtgcaggt ggagactatc tccccaggag
     3181 acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg
     3241 aagatggaaa gaaagttgat tcctcccggg acagaaacaa gccctttaag tttatgctag
     3301 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga
     3361 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca
     3421 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa tctggcggtg
     3481 gatccggagt cgacggattt ggtgatgtcg gtgctcttga gagtttgagg ggaaatgcag
     3541 atttggctta catcctgagc atggagccct gtggccactg cctcattatc aacaatgtga
     3601 acttctgccg tgagtccggg ctccgcaccc gcactggctc caacatcgac tgtgagaagt
     3661 tgcggcgtcg cttctcctcg ctgcatttca tggtggaggt gaagggcgac ctgactgcca
     3721 agaaaatggt gctggctttg ctggagctgg cgcggcagga ccacggtgct ctggactgct
     3781 gcgtggtggt cattctctct cacggctgtc aggccagcca cctgcagttc ccaggggctg
     3841 tctacggcac agatggatgc cctgtgtcgg tcgagaagat tgtgaacatc ttcaatggga
     3901 ccagctgccc cagcctggga gggaagccca agctcttttt catccaggcc tgtggtgggg
     3961 agcagaaaga ccatgggttt gaggtggcct ccacttcccc tgaagacgag tcccctggca
     4021 gtaaccccga gccagatgcc accccgttcc aggaaggttt gaggaccttc gaccagctgg
     4081 acgccatatc tagtttgccc acacccagtg acatctttgt gtcctactct actttcccag
     4141 gttttgtttc ctggagggac cccaagagtg gctcctggta cgttgagacc ctggacgaca
     4201 tctttgagca gtgggctcac tctgaagacc tgcagtccct cctgcttagg gtcgctaatg
     4261 ctgtttcggt gaaagggatt tataaacaga tgcctggttg ctttaatttc ctccggaaaa
     4321 aacttttctt taaaacatca gtcgactatc cgtacgacgt accagactac gcactcgacg
     4381 gaagtggagc taccaatttt agtctgctga agcaggccgg cgacgtggag gagaaccccg
     4441 gccccatgtc tcgctccgtg gccttagctg tgctcgcgct actctctctt tctggcctgg
     4501 aggctgtgat ggcgccgaga acgctaatac taggatgcgg tgctagcggt ggtggtggta
     4561 gcggaggtgg aggcagcatc cagcgtactc caaagattca ggtttactca cgtcatccag
     4621 cagagaatgg aaagtcaaat ttcctgaatt gctatgtgtc tgggtttcat ccatccgaca
     4681 ttgaagttga cttactgaag aatggagaga gaattgaaaa agtggagcat tcagacttgt
     4741 ctttcagcaa ggactggtct ttctatctct tgtactacac tgaattcacc cccactgaaa
     4801 aagatgagta tgcctgccgt gtgaaccatg tgactttgtc acagcccaag atagttaagt
     4861 gggatcgaga catgggcggt ggtggttccg gtggaggcgg ttccggaggt ggtggatccg
     4921 gtggtggagg tagtggctcc cactccttga agtatttcca cacttccgtg tcccggcccg
     4981 gccgcgggga gccccgcttc atctctgtgg gctacgtgga cgacacccag ttcgtgcgct
     5041 tcgacaacga cgccgcgagt ccgaggatgg tgccgcgggc gccgtggatg gagcaggagg
     5101 ggtcagagta ttgggaccgg gagacacgga gcgccaggga caccgcacag attttccgag
     5161 tgaatctgcg gacgctgcgc ggctgctaca atcagagcga ggccgggtct cacaccctgc
     5221 agtggatgca tggctgcgag ctggggcccg acgggcgctt cctccgcggg tatgaacagt
     5281 tcgcctacga cggcaaggat tatctcaccc tgaatgagga cctgcgctcc tggaccgcgg
     5341 tggacacggc ggctcagatc tccgagcaaa agtcaaatga tgcctctgag gcggagcacc
     5401 agagagccta cctggaagac acatgcgtgg agtggctcca caaatacctg gagaagggga
     5461 aggagacgct gcttcacctg gagcccccaa agacacacgt gactcaccac cccatctctg
     5521 accatgaggc caccctgagg tgctgggccc tgggcttcta ccctgcggag atcacactga
     5581 cctggcagca ggatggggag ggccataccc aggacacgga gctcgtggag accaggcctg
     5641 caggggatgg aaccttccag aagtgggcag ctgtggtggt gccttctgga gaggagcaga
     5701 gatacacgtg ccatgtgcag catgaggggc tacccgagcc cgtcaccctg agatggaagc
     5761 cggcttccca gcccaccatc cccatcgtgg gcatcattgc tggcctggtt ctccttggat
     5821 ctgtggtctc tggagctgtg gttgctgctg tgatatggag gaagaagagc tcaggtggaa
     5881 aaggagggag ctactctaag gctgagtgga gcgacagtgc ccaggggtct gagtctcaca
     5941 gcttgggaag cggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc
     6001 ctggacctat gaccgtcgcg cggccgagcg tgcccgcggc gctgcccctc ctcggggagc
     6061 tgccccggct gctgctgctg gtgctgttgt gcctgccggc cgtgtggggt gactgtggcc
     6121 ttcccccaga tgtacctaat gcccagccag ctttggaagg ccgtacaagt tttcccgagg
     6181 atactgtaat aacgtacaaa tgtgaagaaa gctttgtgaa aattcctggc gagaaggact
     6241 cagtgatctg ccttaagggc agtcaatggt cagatattga agagttctgc aatcgtagct
     6301 gcgaggtgcc aacaaggcta aattctgcat ccctcaaaca gccttatatc actcagaatt
     6361 attttccagt cggtactgtt gtggaatatg agtgccgtcc aggttacaga agagaacctt
     6421 ctctatcacc aaaactaact tgccttcaga atttaaaatg gtccacagca gtcgaatttt
     6481 gtaaaaagaa atcatgccct aatccgggag aaatacgaaa tggtcagatt gatgtaccag
     6541 gtggcatatt atttggtgca accatctcct tctcatgtaa cacagggtac aaattatttg
     6601 gctcgacttc tagtttttgt cttatttcag gcagctctgt ccagtggagt gacccgttgc
     6661 cagagtgcag agaaatttat tgtccagcac caccacaaat tgacaatgga ataattcaag
     6721 gggaacgtga ccattatgga tatagacagt ctgtaacgta tgcatgtaat aaaggattca
     6781 ccatgattgg agagcactct atttattgta ctgtgaataa tgatgaagga gagtggagtg
     6841 gcccaccacc tgaatgcaga ggaaaatctc taacttccaa ggtcccacca acagttcaga
     6901 aacctaccac agtaaatgtt ccaactacag aagtctcacc aacttctcag aaaaccacca
     6961 caaaaaccac cacaccaaat gctcaagcaa cacggagtac acctgtttcc aggacaacca
     7021 agcattttca tgaaacaacc ccaaataaag gaagtggaac cacttcaggt actacccgtc
     7081 ttctatctgg gcacacgtgt ttcacgttga caggtttgct tgggacgcta gtaaccatgg
     7141 gcttgctgac tggaagcgga cagtgtacta attatgctct cttgaaattg gctggagatg
     7201 ttgagagcaa ccctggacct accatgattg aacaagatgg attgcacgca ggttctccgg
     7261 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7321 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     7381 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     7441 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     7501 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     7561 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     7621 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     7681 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     7741 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     7801 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     7861 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     7921 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     7981 gcatcgcctt ctatcgcctt cttgacgagt tcttctaacc gctgatcagc ctcgactgtg
     8041 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8101 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8161 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8221 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     8281 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcatctag cggccgatca
     8341 ggcccgacct actctcttcc gcattggagt cgctttaact ggccctggct ttggcagcct
     8401 gtgctgaccc atgcagtcct ccttaccatc cctccctcga cttcccctct tccgatgttg
     8461 agcccctcca gccggtcctg gactttgtct ccttccctgc cctgccctct cctgaacctg
     8521 agccagctcc catagctcag tctggtctat ctgcctggcc ctggccattg tcactttgcg
     8581 ctgccctcct ctcgcccccg agtgcccttg ctgtgccgcc ggaactctgc cctctaacgc
     8641 tgccgtctct ctcctgagtc cggaccactt tgagctctac tggcttctgc gccgcctctg
     8701 gcccactgtt tccccttccc aggcaggtcc tgctttctct gacctgcatt ctctcccctg
     8761 ggcctgtgcc gctttctgtc tgcagcttgt ggcctgggtc acctctacgg ctggcccaga
     8821 tccttccctg ccgcctcctt caggttccgt cttcctccac tccctcttcc ccttgctctc
     8881 tgctgtgttg ctgcccaagg atgctctttc cggagcactt ccttctcggc gctgcaccac
     8941 gtgatgtcct ctgagcggat cctccccgtg tctgggtcct ctccgggcat ctctcctccc
     9001 tcacccaacc ccatgccgtc ttcactcgct gggttccctt ttccttctcc ttctggggcc
     9061 tgtgccatct ctcgtttctt aggatggcct tctccgacgg atgtctccct tgcgtcccgc
     9121 ctccccttct tgtaggcctg catcaggcca aattggccgt cgactgcaga ggcctgcatg
     9181 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9241 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9301 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9361 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9421 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9481 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9541 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9601 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9661 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9721 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9781 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9841 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9901 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9961 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10021 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10081 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10141 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10201 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10261 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10321 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10381 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10441 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10501 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10561 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10621 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10681 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10741 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10801 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10861 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10921 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10981 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11041 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11101 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11161 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11221 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11281 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11341 aagtgccac
//
