LOCUS       sequence-409032-       11006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of GFP-dnMCAK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   Hong C, Schubert M, Tijhuis AE, Requesens M, Roorda M, van den Brink
            A, Ruiz LA, Bakker PL, van der Sluis T, Pieters W, Chen M, Wardenaar
            R, van der Vegt B, Spierings DCJ, de Bruyn M, van Vugt MATM, Foijer 
            F
  TITLE     cGAS-STING drives the IL-6-dependent survival of chromosomally 
            instable cancers.
  JOURNAL   Nature. 2022 Jul;607(7918):366-373. doi: 10.1038/s41586-022-04847-2.
            Epub 2022 Jun 15.
  PUBMED    35705809
REFERENCE   2  (bases 1 to 11006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04847-2"; journalName: "Nature"; date: 
            "2022-07"; volume: "607"; issue: "7918"; pages: "366-373"
COMMENT     Sequence Label: pLV-CMV-dnMCAK
FEATURES             Location/Qualifiers
     source          1..11006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2214..2593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2594..2797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2748..2768
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2836..2845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2842..2844,2845..2847,2848..3558)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2842..2844,2845..2847,2848..3558)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2887..2908)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3148..3167)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3495..3516
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5838..6337
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5873..5896)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6270..6289
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6358..6957
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6358..6377)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6854..6874
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6974..7562
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7027..7047)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7445..7456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7769..8402
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8530..8546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8530..8546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8543..8565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8554..8570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8578..8584,8585..8602,8603..8608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8623..8644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8761..8778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8932..9520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9012..9031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9691..10482,10483..10551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10314..10333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10552..10656)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10704..10838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10741..10760)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10795..10814
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11006,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagcgc gttgacattg
     2221 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     2281 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2341 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2401 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2461 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2521 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2581 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2641 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2701 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2761 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
     2821 ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2881 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2941 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3001 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3061 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3121 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3181 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3241 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3301 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3361 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3421 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     3481 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3541 atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgaa ttcgaggcgc
     3601 gccatggagt cgcttcccgc tcgattgttt cctggtctct ccatcaagat tcaacgcagt
     3661 aatggcttaa ttcacagtgc caacataagc acagtgaacg tggagaaatc ctgtgtttca
     3721 gtggaatgga tagaaggagg taacacgaaa ggcaaagaga ttgattttga tgatgtggct
     3781 gcaataaacc cagaacttct acagcttctt cccttacacc caaaggacaa tctgccactg
     3841 caggaaaatg taacagttcc gaagcaaaaa cgcagagcag tcaactccaa aattccagct
     3901 ccaaaagaag gtcttcgagc acgtgcaact cgcatggcaa ctgtcccaga ggttcgaatt
     3961 gccactcagg agaatgaaat ggaggtggag ctgcctgtgg ctacaaactc tcgaaagcag
     4021 ttttcggttg ccactggcct ccctaggccc tcctgtcctg caatgacaga attaccgttg
     4081 tcgatggtca gcgaggaggc agaagagcaa gtccatccca cccgaagcac atcttctgca
     4141 aaccctgctc ggaggaaagc atgtattgtg aaggaaatgg aaaaaatgaa gaacaagcgg
     4201 gaagagaaga gggcccagaa ctcggaaata agaataaagc gagctcagga atatgacagc
     4261 agctttccaa actgggaatt tgcccggatg attaaagaat tccgggttac tatagaatgt
     4321 cacccactta ctctgactga tcctacggaa gagcacagga tctgcgtgtg tgtgaggaag
     4381 cgcccactga ataagcaaga actggccaag aaagaaatcg atgtgatttc tgttcccagc
     4441 aagtgtttgc tctttgtgca tgaacccaaa ttgaaagtgg acttgacaaa gtatctggag
     4501 aatcaggcat tctgcttcga ttttgccttt gatgaaacag cttcaaatga agtcgtctac
     4561 aggttcacag caaggccact ggtacagacg atttttgaag ggggaaaagc aacctgcttt
     4621 gcctatgggc agacgggcag tgggaagact cacacaatgg gtggagacct gtctggtaaa
     4681 tctcagaata catctaaagg gatctatgca atggcttccc gggatgtctt ccttctgaag
     4741 agtcagcctc gctaccggaa cttaaacttg gaagtttatg tgacattctt tgagatctac
     4801 aatgggaagg tgtttgatct gctcaacaag aaggccaagc tacgtgtact agaagacagc
     4861 aagcagcagg tgcaggttgt gggacttcag gagtacctgg ttaactgtgc tgatgatgtc
     4921 atcaagatgc tcaacatggg cagtgcctgc aggacttctg gacagacttt tgccaactcc
     4981 aattcttccc gctcccatgc ctgcttccag attcttcttc gagccaaagg gagattgcat
     5041 ggcaaattct ccttggtgga tctggcaggg aatgaacgag gggctgacac ttctagtgct
     5101 gaccgccaga ctcgcatgga gggtgcagag attaacaagt ctctcctagc actgaaggag
     5161 tgcatcaggg cgctgggaca gaacaaggct gccaccccat tcgctgagag cgcactgact
     5221 caggtgctaa gggattcatt cattggggag aactcgagga cttgcatgat tgccatgatc
     5281 tcaccaggca taagctcctg tgaatatact ttaaacacgc tgagatatgc agacagagtc
     5341 aaggagctga gcccccacag cgggctcagt ggagagcagc caattcaaat ggaaacggaa
     5401 gagatggaag ccagctctaa tgggacctcg ctggcagtca atttcaagga agaagaggag
     5461 ctgtcttccc agatgtccag ctttaatgaa gccatgagtc agatcaggga gctggaagag
     5521 agggccatgg aggagctcag ggagatcata cagcaaggac caggctggct tgagctctct
     5581 gagatgactg atcagccaga ctatgactta gagacctttg tgaacaaagc agaatctgcc
     5641 ctgactcagc agactaagca cttctcagcc ctgcgagaag tcatcaaggc cctgcgtgtg
     5701 gccatgcaac tggaagaaca ggctagcaaa cagatgaaca gcaagaaacg gcaccaatga
     5761 ttaattaact cgacggtacc gcgggcccgg gatccgcggc cgcgccggct ctagatcgcg
     5821 aacgcgtgaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     5881 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     5941 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     6001 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     6061 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     6121 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     6181 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     6241 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     6301 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     6361 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6421 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6481 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6541 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6601 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6661 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6721 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6781 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6841 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6901 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgg
     6961 gcgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7021 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7081 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7141 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7201 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7261 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7321 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7381 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7441 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7501 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7561 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     7621 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     7681 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7741 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     7801 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     7861 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     7921 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     7981 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     8041 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     8101 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     8161 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     8221 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8281 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8341 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8401 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     8461 agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc
     8521 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8581 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8641 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8701 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8761 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8821 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8881 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8941 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9001 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9061 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9121 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9181 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9241 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9301 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9361 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9421 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9601 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9661 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9721 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9781 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9841 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9901 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9961 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10021 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10081 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10141 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10201 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10261 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10321 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10381 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10441 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10501 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10561 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10621 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10681 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
    10741 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10801 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    10861 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    10921 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10981 aaatatgtac tacaaactta gtagtt
//
