LOCUS       sequence-409066-        3559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  J23107.MCP-SoxS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Alba Burbano D, Cardiff RAL, Tickman BI, Kiattisewee C, Maranas CJ, 
            Zalatan JG, Carothers JM
  TITLE     Engineering activatable promoters for scalable and multi-input 
            CRISPRa/i circuits.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jul 25;120(30):e2220358120. doi: 
            10.1073/pnas.2220358120. Epub 2023 Jul 18.
  PUBMED    37463216
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-07-25"; volume: "120(30):e2220358120. 
            doi"; pages: " 10.1073/pnas.2220358120. Epub 2023 Jul 18"
COMMENT     Sequence Label: pRC029
FEATURES             Location/Qualifiers
     source          1..3559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(448..1239,1240..1308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1071..1090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1309..1413)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1481..1499
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1851..1885
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     RBS             1895..1906
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(3077..3094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3248..3559,1..277))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3328..3347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
       61 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      121 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      181 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      241 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      301 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      361 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      421 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      481 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
      541 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
      601 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
      661 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
      721 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
      781 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
      841 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
      901 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
      961 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1021 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     1081 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1141 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1201 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     1261 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     1321 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     1381 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     1441 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     1501 ctttcgtctt caagaattct ggcgaatcct ctgaccagcc agaaaacgac ctttctgtgg
     1561 tgaaaccgga tgctgcaatt cagagcgcca gcaagtgggg gacagcagaa gacctgaccg
     1621 ccgcagagtg gatgtttgac atggtgaaga ctatcgcacc atcagccaga aaaccgaatt
     1681 ttgctgggtg ggctaacgat atccgcctga tgcgtgaacg tgacggacgt aaccaccgcg
     1741 acatgtgtgt gctgttccgc tgggcatgcc aggacaactt ctggtccggt aacgtgctga
     1801 gcccggccaa gcttagcccg aaaagtgcca cctgtggcaa ttccgacgtc tttacggcta
     1861 gctcagccct aggtattatg ctagcgaatt cattaaagag gagaaaggta ccatggggcc
     1921 cgcttctaac tttactcagt tcgttctcgt cgacaatggc ggaactggcg acgtgactgt
     1981 cgccccaagc aacttcgcta acgggatcgc tgaatggatc agctctaact cgcgttcaca
     2041 ggcttacaaa gtaacctgta gcgttcgtca gagctctgcg cagaatcgca aatacaccat
     2101 caaagtcgag gtgcctaaag gcgcctggcg ttcgtactta aatatggaac taaccattcc
     2161 aattttcgcc acgaattccg actgcgagct tattgttaag gcaatgcaag gtctcctaaa
     2221 agatggaaac ccgattccct cagcaatcgc agcaaactcc ggcatctacg gtggcggagg
     2281 tagcatgtcc catcagaaaa ttattcagga tcttatcgca tggattgacg agcatattga
     2341 ccagccgctt aacattgatg tagtcgcaaa aaaatcaggc tattcaaagt ggtacttgca
     2401 acgaatgttc cgcacggtga cgcatcagac gcttggcgat tacattcgcc aacgccgcct
     2461 gttactggcc gccgttgagt tgcgcaccac cgagcgtccg atttttgata tcgcaatgga
     2521 cctgggttat gtctcgcagc agaccttctc ccgcgttttc gcgcggcagt ttgatcgcac
     2581 tcccgcggat tatcgccacc gcctgtaagc ggccgccacg caaaaaaccc cgcttcggcg
     2641 gggttttttc gcggatcctt actccgtcga caagcttgcg gccgcactcg aggaattccg
     2701 actcaattag ttcagtcagt ttcaggatat tagtcatctc tacattgatt atgagtattc
     2761 agaaattcct taaatattct gacaaatgct ctttccctaa actcccccca taaaaaaacc
     2821 cgccgaagcg ggtttttacg ttatttgcgg attaacgatt actcgttatc agaaccgccc
     2881 agacctgcgt tcagcagttc tgccaggctg gcagatgcgt cttccggaat tgatccgtcg
     2941 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     3001 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     3061 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3241 tggcgttttt ccataggctc cgcacccctg acgagcatca caaaaatcga cgctcaagtc
     3301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3541 ccggtaacta tcgtcttga
//
