LOCUS       sequence-409067-        3755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mRFP with synthetic promoter HP3 (from Figure S21).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Alba Burbano D, Cardiff RAL, Tickman BI, Kiattisewee C, Maranas CJ, 
            Zalatan JG, Carothers JM
  TITLE     Engineering activatable promoters for scalable and multi-input 
            CRISPRa/i circuits.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jul 25;120(30):e2220358120. doi: 
            10.1073/pnas.2220358120. Epub 2023 Jul 18.
  PUBMED    37463216
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-07-25"; volume: "120(30):e2220358120. 
            doi"; pages: " 10.1073/pnas.2220358120. Epub 2023 Jul 18"
COMMENT     Sequence Label: pDA303.DA9.1B.mRFP
FEATURES             Location/Qualifiers
     source          1..3755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..92
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(311..415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     483..501
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             1067..1078
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1085..1762
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2275..2292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2446..3034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2526..2545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3205..3755,1..241,242..310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
       61 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      121 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      181 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      241 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      301 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      361 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      421 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      481 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctggcgaatc ctctgaccag
      541 ccagaaaacg acctttctgt ggtgaaaccg gatgctgcaa ttcagagcgc cagcaagtgg
      601 gggacagcag aagacctgac cgccgcagag tggatgtttg acatggtgaa gactatcgca
      661 ccatcagcca gaaaaccgaa ttttgctggg tgggctaacg atatccgcct gatgcgtgaa
      721 cgtgacggac gtaaccaccg cgacatgtgt gtgctgttcc gctgggcatg ccaggacaac
      781 ttctggtccg gtaacgtgct gagcccggcc aagcttagcc cgaaaagtgc cacctgtggc
      841 aattccgacg tcagcatttg cgatcattca cgcagcgctt attcagttgc tcactgcgat
      901 gtcataatca tcgctacgag ctgtgaaaga tgcataaagc tcgtacgacg cgttcgctcg
      961 tctcctcact tctcctaact ctcacacgtg gctgcaccgc gggcggccgc tgccggggcg
     1021 cgttgacgcc tccttctttc ttagggattg tgctagcgaa ttcattaaag aggagaaagg
     1081 taccatggcg agtagcgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
     1141 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
     1201 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
     1261 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
     1321 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
     1381 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
     1441 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
     1501 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
     1561 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
     1621 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     1681 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     1741 tcgtcactcc accggtgctt aaggatccaa actcgagtaa ggatctgtgc tttttttaac
     1801 gcatgagaaa gcccccggaa gatcaccttc cgggggcttt tttattgcgc ggatccttac
     1861 tccgtcgaca agcttgcggc cgcactcgag gaattccgac tcaattagtt cagtcagttt
     1921 caggatatta gtcatctcta cattgattat gagtattcag aaattcctta aatattctga
     1981 caaatgctct ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt
     2041 atttgcggat taacgattac tcgttatcag aaccgcccag acctgcgttc agcagttctg
     2101 ccaggctggc agatgcgtct tccggaattg atccgtcgac cgatgccctt gagagccttc
     2161 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     2221 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     2281 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2341 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2401 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2461 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2521 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2581 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2641 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2701 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2761 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2821 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2881 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2941 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3001 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3061 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3121 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3181 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3241 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3301 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3361 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3421 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3481 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3541 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3601 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3661 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3721 catgccatcc gtaagatgct tttctgtgac tggtg
//
