LOCUS       sequence-409073-       10207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0409
FEATURES             Location/Qualifiers
     source          1..10207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(135..179)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    364..597
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1090..1215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1262..1442
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1443..1669)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1697..1717)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1697..1715)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1736..1752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1736..1752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1749..1771)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1760..1776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1784..1790,1791..1808,1809..1814))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1829..1850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1967..1984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2138..2726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2218..2237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2897..3688,3689..3757))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3520..3539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3758..3862)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3888..4343)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4025..4046)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4237..4256
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4470..4492
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4484..4501
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4485..4501
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4511..4530
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4511..4529
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4550..4685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4725..4846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4736..4755)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4790..4809
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4924..5157
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5229..5817
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5335..5346
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5744..5764
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      7763..7772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7783..8961)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7792..8730
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7815..7835)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9099..9216
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9259..9861)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9345..9365)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9842..9861
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(9893..10089)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(9901..10111)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9907..10042
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
       61 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
      121 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
      181 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
      241 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
      301 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
      361 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
      421 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
      481 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
      541 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
      601 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
      661 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
      721 tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt
      781 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
      841 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
      901 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
      961 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
     1021 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
     1081 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
     1141 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
     1201 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca
     1261 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     1321 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     1381 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     1441 ccgtttatgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg caattcagtg
     1501 gttcgtccaa tccatgtcag acccgtctgt tgccttccta ataaggcacg atcgtaccac
     1561 cttacttcca ccaatcggca tgcacggtgc tttttctctc cttgtaaggc atgttgctaa
     1621 ctcatcgtta ccatgttgca agactacaag agtattgcat aagactacat taagcttgca
     1681 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     1741 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1801 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1861 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1921 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1981 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2041 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2221 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2281 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2341 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2401 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2461 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2521 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2581 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2641 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2761 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2821 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2881 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2941 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3001 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3061 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3121 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3181 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3241 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3301 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3361 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3421 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3481 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3541 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3601 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3661 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3721 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3781 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3841 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     3901 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3961 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     4021 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4081 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4141 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4201 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     4261 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     4321 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     4381 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     4441 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     4501 tgagcgcgcg taatacgact cactataggg cgaattgggt acgtccctag gcctccaaaa
     4561 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     4621 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     4681 gggatgggcg gagttagggg cgggatagct agagccagac atgataagat acattgatga
     4741 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4801 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttcctc tcactctctg
     4861 atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa ctactatacg
     4921 tactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     4981 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     5041 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     5101 acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc
     5161 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     5221 aagtcgacgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
     5281 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
     5341 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
     5401 cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag
     5461 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
     5521 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
     5581 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
     5641 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
     5701 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     5761 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattgtc
     5821 gacttaacgc gtacgtctag aatcatatgc cacacgtcgt cctttccaca agatatataa
     5881 agccaagaaa tcgaaatact ttcaagttac ggactagttc attactactt atccatcgtg
     5941 ttgaggagca tttcacaggt cacgccttca tccacttcct ctttgactct ggctgtggtt
     6001 tctctgcagg cgacaggaaa catctctcct gggaactgtt ctcccttcgt ggtggaagcc
     6061 tcctcagcag cagcttcgtc tccgttcgcg gcttccttcc atcccttcgc agcttccttc
     6121 tgaccctttg tggcttcctt caggatggtc tgcttcctct ttcttgttct tcttcccttt
     6181 cctgaagcag ccgactttgc ttcccctgat ccacacttct ccgaatgtct gtgcaggtta
     6241 atccagcggg aaaagccttt gccacacttg gagcatttgt aaacagtcgg gatgaaattt
     6301 gcatcgtggt atttcctgaa gtgagcgttt agaagttgct tctgtcggaa acatttattg
     6361 caagaaaggc aggtgaatgg tttctctcca gtgtgggtac gaatgtgagc ggtcatatga
     6421 cgttcctgct tgcaggcata actgcagtgt ttgcacttga acctcttctc attcttatga
     6481 gttttctggt gctgaatgag ggcatagcgt tcatggaaga cagcagaaca gtagcggcat
     6541 ttcagctctg cagcgctgta agcatgcaag ttgcgcatat gcacacctga gtgcgttctc
     6601 atgtggcgtt tcagcttgta ggtatctctg ctggcatagc tgcactggca acactgaaag
     6661 gggcgctccc cagtgtggga tcggacatgg cgcttcaatt tacttgcctc cacactggca
     6721 tacttgcaca tggaacattt aaagggtttc tcatgagtat gtttatagcg cctgtgtcgg
     6781 acgagttctc cactggtgac aaatgccatg ttgcagtcgt tacacttgta gggcctggtt
     6841 cctgtgtggg tgttaacatg gttccgcagc agagtgaccg tacggaaggt tttcaggcag
     6901 aggtgacaca ggtgaggctt ctcactggtg tgagttttca tatgacgatt aaaacttgac
     6961 attctagaag aggtgaacat gcagacatca cagtggaagg ttccttttgc tccctttgtc
     7021 tttctttgat tttttgtaga tttggccttt tcagcatctg cttgaccagc tgtaggttga
     7081 tcctcttgtt cttccacatt tgaatttgaa actgtgagaa caatttcgtc acttctttca
     7141 tctcctgaca ttgtttccac aaaaaagagc tgctcctttg ttctttcagc caataactgg
     7201 ttcttctcct gctcttcctc gagcttgatc agtccagtag tttcagccag gctcaccgct
     7261 aacttactgt cttcactggc caccatcaca ttctcctcta gagcgtggaa ctgcaacacc
     7321 tccatctctt gcggggagta cagctcttgc tggatactaa tggccacaca ctgctgcagg
     7381 ctctgccggg gcccttcctc aagccacagc aacccagggc caggctgttg caccaccacc
     7441 tgcacccctt cttgctgctg tatgctcagc aagctcatat cctgcaactc cacagcttca
     7501 gaagtgaagt gcaccgtctg cagggtcagg atgtacttct cgctctcctc cgagggggcc
     7561 agcaccagct ccacttcttc ctccaggacg ctgtcctgga aggccccaga ggtacgctcg
     7621 gcctccaact cactagggct ccgatggtct ttctctctgc acactccgtc tttttcctcc
     7681 tccttcaggc ctttttccgg catcaactcg agttctttga tcttggtgaa ttgctcagaa
     7741 aggacagaga tctcagtggc tgccatggtg gcggtgctag cctcacgaca cctgaaatgg
     7801 aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc caaactcaaa
     7861 aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt cagtctccac
     7921 ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta aagacgacgt
     7981 actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact ccgtggagtc
     8041 acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg actcacccgc
     8101 ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc cgggaagcgg
     8161 ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc agggcggggc
     8221 gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag actacccccg
     8281 tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc
     8341 acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca gatcttggcc
     8401 cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta
     8461 aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc agattcgcac
     8521 gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag gggctcctta
     8581 agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat caagaatcac
     8641 gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg taaagaggcc
     8701 aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa
     8761 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
     8821 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
     8881 ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc
     8941 ccactgacgg gcaccggagc caattcccac tcctttcaag acctagaagg tccattagct
     9001 gcaaagattc ctctctgttt aaaactttat ccatctttgc acctgcaggc ctcgagccgg
     9061 cgccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     9121 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     9181 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     9241 ctcaagatct agaattcgtt aactggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
     9301 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     9361 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     9421 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
     9481 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     9541 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     9601 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccacggcgg cgccgtcgtc
     9661 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     9721 ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc
     9781 gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac
     9841 cgtgggcttg tactcggtca tggtaagctc cggtggtacc tctagaaagc tttttgcaaa
     9901 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     9961 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
    10021 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
    10081 tgagacgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
    10141 ttgctgacta attgagatgc aatgcatggg tcccctcggg gttgggaggt gggtctgaaa
    10201 cgataat
//
