LOCUS       sequence-409079-        3787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  J23107.SoxS-PYL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Alba Burbano D, Cardiff RAL, Tickman BI, Kiattisewee C, Maranas CJ, 
            Zalatan JG, Carothers JM
  TITLE     Engineering activatable promoters for scalable and multi-input 
            CRISPRa/i circuits.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jul 25;120(30):e2220358120. doi: 
            10.1073/pnas.2220358120. Epub 2023 Jul 18.
  PUBMED    37463216
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-07-25"; volume: "120(30):e2220358120. 
            doi"; pages: " 10.1073/pnas.2220358120. Epub 2023 Jul 18"
COMMENT     Sequence Label: pRC027
FEATURES             Location/Qualifiers
     source          1..3787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(185..289)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     357..375
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             1679..1690
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(1700..1734)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     primer_bind     complement(2181..2198)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2352..2940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2432..2451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3111..3787,1..115,116..184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3734..3753
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
       61 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      301 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      361 ggcgtatcac gaggcccttt cgtcttcaag aattctggcg aatcctctga ccagccagaa
      421 aacgaccttt ctgtggtgaa accggatgct gcaattcaga gcgccagcaa gtgggggaca
      481 gcagaagacc tgaccgccgc agagtggatg tttgacatgg tgaagactat cgcaccatca
      541 gccagaaaac cgaattttgc tgggtgggct aacgatatcc gcctgatgcg tgaacgtgac
      601 ggacgtaacc accgcgacat gtgtgtgctg ttccgctggg catgccagga caacttctgg
      661 tccggtaacg tgctgagccc ggccaagctt agcccgaaaa gtgccacctg tggcaattcc
      721 gacgtcgatt tcctgcccgg ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgggcgg
      781 ccgcttagtt catagcttca gtgatcgaag caagtttctg aagattcaat ctaataaccg
      841 tatcagcaaa caatctcgta tcttcctccg aattaccttc cggtacatca acaacataag
      901 attccaaaac aacggtccag atcctttctt cttcttcttc tttctcaaat ctatgaaccg
      961 tcgtaaccga tttataattc ctcagcctat gttcaccacc ggttatacta aacccagtca
     1021 ctctccgatc atcgtccaac agatctaatc tctctcgaga cgtattcgcc ggtaatccac
     1081 ttatcacgtt cacgtcgcgc gtgcatccca ctcgcatctc gaaatcttca ctcacgttac
     1141 agcttttgat gaagtgtttg taaatctgtg gcctatcgaa acgtctcacc acggaccata
     1201 ctgtttccgg cggcgcgtgg attcgctgag ctaggagaga tgagcaacgg ccgttaccga
     1261 gttggtacgt gtggaactcg gcgattgatt gggagagttg ggtgaattcg tcttgagttg
     1321 gcgcgccccc acccatgcta cctccgccac ccaggcggtg gcgataatcc gcgggagtgc
     1381 gatcaaactg ccgcgcgaaa acgcgggaga aggtctgctg cgagacataa cccaggtcca
     1441 ttgcgatatc aaaaatcgga cgctcggtgg tgcgcaactc aacggcggcc agtaacaggc
     1501 ggcgttggcg aatgtaatcg ccaagcgtct gatgcgtcac cgtgcggaac attcgttgca
     1561 agtaccactt tgaatagcct gatttttttg cgactacatc aatgttaagc ggctggtcaa
     1621 tatgctcgtc aatccatgcg ataagatcct gaataatttt ctgatgggac atggtacctt
     1681 tctcctcttt aatgaattcg ctagcataat acctagggct gagctagccg taaaatgcat
     1741 cctagggata tattccggat ccttactccg tcgacaagct tgcggccgca ctcgaggaat
     1801 tccgactcaa ttagttcagt cagtttcagg atattagtca tctctacatt gattatgagt
     1861 attcagaaat tccttaaata ttctgacaaa tgctctttcc ctaaactccc cccataaaaa
     1921 aacccgccga agcgggtttt tacgttattt gcggattaac gattactcgt tatcagaacc
     1981 gcccagacct gcgttcagca gttctgccag gctggcagat gcgtcttccg gaattgatcc
     2041 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     2101 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     2161 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2221 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2281 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2341 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2461 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2521 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2581 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2641 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2701 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2761 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2821 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2881 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2941 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3001 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3061 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3121 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3181 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3241 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3301 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3361 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3421 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3481 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3541 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3601 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3661 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3721 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3781 aaacgtt
//
