LOCUS       sequence-409083-       11242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered 
            BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(deltaI)
FEATURES             Location/Qualifiers
     source          1..11242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             990..1016
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1020..1724
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     CDS             4845..6695
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      6459..6468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7194..7211)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7200..7424
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7557..7576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7708..7755
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7763..8785
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7763..8785
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8915..9036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8952..8971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9006..9025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9085..9101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9085..9101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9098..9120)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9109..9125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9133..9139,9140..9157,9158..9163))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9178..9199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9316..9333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9487..10075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9567..9586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10246..11037,11038..11106))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10869..10888
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11107..11211)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatgggac cggctgccaa gcgcgtgaaa ctcgatggcg
     1021 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
     1081 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
     1141 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
     1201 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
     1261 cgcctttcca ggccgcaatg gtagatggct ccggatacca agtccatcgc acaatgcagt
     1321 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
     1381 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
     1441 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
     1501 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
     1561 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
     1621 acgtgttccg taagacggaa ctcaagcact ccaagaccga actcaacttc aaggagtggc
     1681 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagggcggc tcgtacgtgt
     1741 acaagtctgc ggagagcggc cctgggacga gattgagaaa tctgccagta atgggggatg
     1801 gactagaaac ttcccaaatg tctacaacac aggcccaggc ccaaccccag ccagccaacg
     1861 cagccagcac caaccccccg cccccagaga cctccaaccc taacaagccc aagaggcaga
     1921 ccaaccaact gcaatacctg ctcagagtgg tgctcaagac actatggaaa caccagtttg
     1981 catggccttt ccagcagcct gtggatgccg tcaagctgaa cctccctgat tactataaga
     2041 tcattaaaac gcctatggat atgggaacaa taaagaagcg cttggaaaac aactattact
     2101 ggaatgctca ggaatgtatc caggacttca acactatgtt tacaaattgt tacatctaca
     2161 acaagcctgg agatgacata gtcttaatgg cagaagctct ggaaaagctc ttcttgcaaa
     2221 aaataaatga gctacccaca gaagaaaccg agatcatgat agtccaggca aaaggaagag
     2281 gacgtgggag gaaagaaaca gggacagcaa aacctggcgt ttccacggta ccaaacacaa
     2341 ctcaagcatc gactcctccg cagacccaga cccctcagcc gaatcctcct cctgtgcagg
     2401 ccacgcctca ccccttccct gccgtcaccc cggacctcat cgtccagacc cctgtcatga
     2461 cagtggtgcc tccccagcca ctgcagacgc ccccgccagt gcccccccag ccacaacccc
     2521 cacccgctcc agctccccag cccgtacaga gccacccacc catcatcgcg gccaccccac
     2581 agcctgtgaa gacaaagaag ggagtgaaga ggaaagcaga caccaccacc cccaccacca
     2641 ttgaccccat tcacgagcca ccctcgctgc ccccggagcc caagaccacc aagctgggcc
     2701 agcggcggga gagcagccgg cctgtgaaac ctccaaagaa ggacgtgccc gactctcagc
     2761 agcacccagc accagagaag agcagcaagg tctcggagca gctcaagtgc tgcagcggca
     2821 tcctcaagga gatgtttgcc aagaagcacg ccgcctacgc ctggcccttc tacaagcctg
     2881 tggacgtgga ggcactgggc ctacacgact actgtgacat catcaagcac cccatggaca
     2941 tgagcacaat caagtctaaa ctggaggccc gtgagtaccg tgatgctcag gagtttggtg
     3001 ctgacgtccg attgatgttc tccaactgct ataagtacaa ccctcctgac catgaggtgg
     3061 tggccatggc ccgcaagctc caggatgtgt tcgaaatgcg ctttgccaag atgccggacg
     3121 agcctgagga gccagtggtg gccgtgtcct ccccggcagt gccccctccc accaaggttg
     3181 tggccccgcc ctcatccagc gacagcagca gcgatagctc ctcggacagt gacagttcga
     3241 ctgatgactc tgaggaggag cgagcccagc ggctggctga gctccaggag cagctcaaag
     3301 ccgtgcacga gcagcttgca gccctctctc agccccagca gaacaaacca aagaaaaagg
     3361 agaaagacaa gaaggaaaag aaaaaagaaa agcacaaaag gaaagaggaa gtggaagaga
     3421 ataaaaaaag caaagccaag gaacctcctc ctaaaaagac gaagaaaaat aatagcagca
     3481 acagcaatgt gagcaagaag gagccagcgc ccatgaagag caagccccct cccacgtatg
     3541 agtcggagga agaggacaag tgcaagccta tgtcctatga ggagaagcgg cagctcagct
     3601 tggacatcaa caagctcccc ggcgagaagc tgggccgcgt ggtgcacatc atccagtcac
     3661 gggagccctc cctgaagaat tccaaccccg acgagattga aatcgacttt gagaccctga
     3721 agccgtccac actgcgtgag ctggagcgct atgtcacctc ctgtttgcgg aagaaaagga
     3781 aacctcaagc tgagaaagtt gatgtgattg ccggctcctc caagatgaag ggcttctcgt
     3841 cctcagagtc ggagagctcc agtgagtcca gctcctctga cagcgaagac tccgaaacag
     3901 atccagagaa gcgcaagctc atccagcagc agcttgttct ccttttgcat gctcacaagt
     3961 gccagcgccg ggaacaggcc aatggggaag tgaggcagtg caaccttccc cactgtcgca
     4021 caatgaagaa tgtcctaaac cacatgacac actgccagtc aggcaagtct tgccaagtgg
     4081 cacactgtgc atcttctcga caaatcattt cacactggaa gaattgtaca agacatgatt
     4141 gtcctgtgtg tctccccctc aaaaatgctg gtgataagag aaatcaacag ccaattttga
     4201 ctggagcacc cgttggactt ggaaatccta gctctctagg ggtgggtcaa cagtctgccc
     4261 ccaacctaag cactgttagt cagattgatc ccagctccat agaaagagcc tatgcagctc
     4321 ttggactacc ctatcaagta aatcagatgc cgacacaacc ccaggtgcaa gcaaagaacc
     4381 agcagaatca gcagcctggg cagtctcccc aaggcatgcg gcccatgagc aacatgagtg
     4441 ctagtcctat gggagtaaat ggaggtgtag gagttcaaac gccgagtctt ctttctgact
     4501 caatgttgca ttcagccata aattctcaaa acccaatgat gagtgaaaat gccagtgtgc
     4561 cctccatggg tcctatgcca acagcagctc aaccatccac tactggaatt cggaaacagt
     4621 ggcacgaaga tattactcag gatcttcgaa atcatcttgt tcacaaactc gtccaagcca
     4681 tatttcctac gccggatcct gctgctttaa aagacagacg gatggaaaac ctagttgcat
     4741 atgctcggaa agttgaaggg gacatgtatg aatctgcaaa caatcgagcg gaatactacc
     4801 accttctagc tgagaaaatc tataagatcc agaaagaact agaaattttc aaaccagaag
     4861 aactacgaca ggcactgatg ccaactttgg aggcacttta ccgtcaggat ccagaatccc
     4921 ttccctttcg tcaacctgtg gaccctcagc ttttaggaat ccctgattac tttgatattg
     4981 tgaagagccc catggatctt tctaccatta agaggaagtt agacactgga cagtatcagg
     5041 agccctggca gtatgtcgat gatatttggc ttatgttcaa taatgcctgg ttatataacc
     5101 ggaaaacatc acgggtatac aaatactgct ccaagctctc tgaggtcttt gaacaagaaa
     5161 ttgacccagt gatgcaaagc cttggatact gttgtggcag aaagttggag ttctctccac
     5221 agacactgtg ttgctacggc aaacagttgt gcacaatacc tcgtgatgcc acttattaca
     5281 gttaccagaa caggtatcat ttctgtgaga agtgtttcaa tgagatccaa ggggagagcg
     5341 tttctttggg ggatgaccct tcccagcctc aaactacaat aaataaagaa caattttcca
     5401 agagaaaaaa tgacacactg gatcctgaac tgtttgttga atgtacagag tgcggaagaa
     5461 agatgcatca gatctgtgtc cttcaccatg agatcatctg gcctgctgga ttcgtctgtg
     5521 atggctgttt aaagaaaagt gcacgaacta ggaaagaaaa taagttttct gctaaaaggt
     5581 tgccatctac cagacttggc acctttctag agaatcgtgt gaatgacttt ctgaggcgac
     5641 agaatcaccc tgagtcagga gaggtcactg ttagagtagt tcatgcttct gacaaaaccg
     5701 tggaagtaaa accaggcatg aaagcaaggt ttgtggacag tggagagatg gcagaatcct
     5761 ttccataccg aaccaaagcc ctctttgcct ttgaagaaat tgatggtgtt gacctgtgct
     5821 tctttggcat gcatgttcaa gagtatggct ctgactgccc tccacccaac cagaggagag
     5881 tatacatatc ttacctcgat agtgttcatt tcttccgtcc taaatgcttg aggactgcag
     5941 tctatcatga aatcctaatt ggatatttag aatatgtcaa gaaattaggt tacacaacag
     6001 ggcatatttg ggcatgtcca ccaagtgagg gagatgatta tatcttccat tgccatcctc
     6061 ctgaccagaa gatacccaag cccaagcgac tgcaggaatg gtacaaaaaa atgcttgaca
     6121 aggctgtatc agagcgtatt gtccatgact acaaggatat ttttaaacaa gctactgaag
     6181 atagattaac aagtgcaaag gaattgcctt atttcgaggg tgatttctgg cccaatgttc
     6241 tggaagaaag cattaaggaa ctggaacagg aggaagaaga gagaaaacga gaggaaaaca
     6301 ccagcaatga aagcacagat gtgaccaagg gagacagcaa aaatgctaaa aagaagaata
     6361 ataagaaaac cagcaaaaat aagagcagcc tgagtagggg caacaagaag aaacccggga
     6421 tgcccaatgt atctaacgac ctctcacaga aactatatgc caccatggag aagcataaag
     6481 aggtcttctt tgtgatccgc ctcattgctg gccctgctgc caactccctg cctcccattg
     6541 ttgatcctga tcctctcatc ccctgcgatc tgatggatgg tcgggatgcg tttctcacgc
     6601 tggcaaggga caagcacctg gagttctctt cactccgaag agcccagtgg tccaccatgt
     6661 gcatgctggt ggagctgcac acgcagagcc aggaccgctt tgtctacacc tgcaatgaat
     6721 gcaagcacca tgtggagaca cgctggcact gtactgtctg tgaggattat gacttgtgta
     6781 tcacctgcta taacactaaa aaccatgacc acaaaatgga gaaactaggc cttggcttag
     6841 atgatgagag caacaaccag caggctgcag ccacccagag cccaggcgat tctcgccgcc
     6901 tgagtatcca gcgctgcatc cagtctctgg tccatgcttg ccagtgtcgg aatgccaatt
     6961 gctcactgcc atcctgccag aagatgaagc gggttgtgca gcataccaag ggttgcaaac
     7021 ggaaaaccaa tggcgggtgc cccatctgca agcagctcat tgccctctgc tgctaccatg
     7081 ccaagcactg ccaggagaac aaatgcccgg tgccgttctg cctaaacatc aagggctcga
     7141 cctgatcata gtgaggctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     7201 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7261 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     7321 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7381 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     7441 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     7501 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7561 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7621 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7681 tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga
     7741 aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     7801 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     7861 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     7921 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     7981 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     8041 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     8101 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     8161 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     8221 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     8281 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     8341 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     8401 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     8461 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     8521 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     8581 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     8641 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     8701 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     8761 cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt
     8821 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8881 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8941 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9001 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     9061 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9121 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9181 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9241 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9301 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9421 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9481 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9541 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9601 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9661 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9721 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9781 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9841 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9901 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9961 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10021 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10081 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10141 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10201 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10261 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10321 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10381 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10441 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10501 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10561 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10621 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10681 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10741 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10801 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10861 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10921 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10981 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11041 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11101 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11161 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11221 ccgaaaagtg ccacctgacg tc
//
