LOCUS       sequence-409091-       15673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of Ms2 protein fused to VPR and dCas9 
            fused to EDLL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M,
            Garcia-Carpintero V, Granell A, Orzaez D
  TITLE     Strong gene activation in plants with genome-wide specificity using 
            a new orthogonal CRISPR/Cas9-based programmable transcriptional 
            activator.
  JOURNAL   Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 
            10.1111/pbi.13138. Epub 2019 May 23.
  PUBMED    31034138
REFERENCE   2  (bases 1 to 15673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13138";
            journalName: "Plant Biotechnol J"; date: "2019-09"; volume: "17"; 
            issue: "9"; pages: "1703-1705"
COMMENT     Sequence Label: pDGB3 omega1 P35S:MS2:VPR:Tnos -
            P35S:dCas9:ED...
FEATURES             Location/Qualifiers
     source          1..15673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             50..1123
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1189..1383
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1619..1641)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1727..1867
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1782..1801
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1882..1899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2053..2641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2133..2152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2729..3520)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    4306..4330
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4964..5308
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5388..5774
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5781..5801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5805..5825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5829..5849
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5850..5999
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6000..6020
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6024..6044
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6045..6401
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6426..6995
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      7022..7274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8118..8462
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8539..8559
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(8560..8583,8584..8586,8587..11070,11071..11076,
                     11077..11142,11143..11145,11146..12660)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(8560..8583,8584..8586,8587..11073,11074..11076,
                     11077..12660)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8560..12660
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             12679..12699
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12703..12723
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12727..12747
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      12846..13098
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13340..13364
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14665..15294
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
       61 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
      121 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
      181 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
      241 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
      301 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
      361 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
      421 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
      481 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
      541 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
      601 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
      661 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
      721 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
      781 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
      841 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
      901 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
      961 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     1021 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     1081 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     1141 agggcaaatt gccctagcag gggaaaaagg tcgaaaagga ctctttcctg tggatagcac
     1201 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     1261 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     1321 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     1381 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     1441 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     1501 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     1561 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     1621 tctgacacat gcagctcccg gtgacggtca cagcttgtct gtaagcggat gccgggagca
     1681 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     1741 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     1801 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1861 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1921 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1981 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2041 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2221 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2341 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2401 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2461 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2521 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2581 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2641 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2701 agggattttg gtcatgcatt ctaggtgatt atttgccgac taccttggtg atctcgcctt
     2761 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
     2821 gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
     2881 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
     2941 aaatgcggga caacgtaagc actacatttc gctcatcacc agcccagtcg ggcggcgagt
     3001 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
     3061 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
     3121 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
     3181 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
     3241 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
     3301 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
     3361 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
     3421 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
     3481 atagttgagt cgatacttcg gcgatcaccg cttccctcat aatgtttaac tttgttttag
     3541 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     3601 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat
     3661 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg
     3721 accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg
     3781 tccactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggta
     3841 gcagcgaagt cgaggcattt ctgtcctggc tggaacagaa cttattattt ccttcctctt
     3901 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
     3961 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
     4021 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
     4081 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     4141 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     4201 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
     4261 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
     4321 tggtgtaaac ataacggatc cggtctcagg agactagagc caagctgatc tcctttgccc
     4381 cggagatcac catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag
     4441 aaggtgacga taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa
     4501 agaatgctga cccacagatg gttagagagg cctacgcggc aggtctgatc aagacgatct
     4561 acccgagtaa taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca
     4621 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa
     4681 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga
     4741 ttggagtctc taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga
     4801 tcgaggatct aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac
     4861 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact
     4921 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
     4981 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
     5041 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
     5101 tcgttcaaga tgcccctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     5161 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     5221 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     5281 aaggaagttc atttcatttg gagaggactc cggtattttt acaacaatta ccacaacaaa
     5341 acaaacaaca aacaacatta caatttacta ttctagtcga aatgatggct tctaatttta
     5401 ctcaatttgt tcttgttgat aatggtggta ctggtgatgt tactgttgct ccttctaatt
     5461 ttgctaatgg tgttgctgaa tggatttctt ctaattctag atctcaagct tataaggtta
     5521 cttgttctgt tagacaatct tctgctcaaa atagaaagta tactattaag gttgaagttc
     5581 ctaaggttgc tactcaaact gttggtggtg ttgaacttcc tgttgctgct tggagatctt
     5641 atcttaatat ggaacttact attcctattt ttgctactaa ttctgattgt gaacttattg
     5701 ttaaggctat gcaaggtctt cttaaggatg gtaatcctat tccttctgct attgctgcta
     5761 attctggtat ttattcggac ccaaaaaaga agcgtaaggt cgatcccaag aagaagagaa
     5821 aggtagatcc taagaagaag agaaaggtag acgcattgga tgacttcgac ttagacatgc
     5881 ttgggtcaga tgctttggac gactttgacc tcgacatgtt gggttctgac gcgctagatg
     5941 acttcgactt ggacatgttg ggctccgacg ctttggacga ctttgatctg gacatgttac
     6001 cgaaaaagaa acgcaaagtt gatcctaaga agaagagaaa ggtacctaca caggccggcg
     6061 agggcacact gtctgaagct ctgctgcagc tgcagttcga cgacgaggat ctgggagccc
     6121 tgctgggaaa cagcaccgat cctgccgtgt tcaccgacct ggccagcgtg gacaacagcg
     6181 agttccagca gctgctgaac cagggcatcc ctgtggcccc tcacaccacc gagcccatgc
     6241 tgatggaata ccccgaggcc atcacccggc tcgtgacagg cgctcagagg cctcctgatc
     6301 cagctcctgc ccctctggga gcaccaggcc tgcctaatgg actgctgtct ggcgacgagg
     6361 acttcagctc tatcgccgat atggatttct cagccttgct gattaactct agaagttccg
     6421 gatctcggga ttccagggaa gggatgtttt tgccgaagcc tgaggccggc tccgctatta
     6481 gtgacgtgtt tgagggccgc gaggtgtgcc agccaaaacg aatccggcca tttcatcctc
     6541 caggaagtcc atgggccaac cgcccactcc ccgccagcct cgcaccaaca ccaaccggtc
     6601 cagtacatga gccagtcggg tcactgaccc cggcaccagt ccctcagcca ctggatccag
     6661 cgcccgcagt gactcccgag gccagtcacc tgttggagga tcccgatgaa gaaacgagcc
     6721 aggctgtcaa agcccttcgg gagatggccg atactgtgat tccccagaag gaagaggctg
     6781 caatctgtgg ccaaatggac ctttcccatc cgcccccaag gggccatctg gatgagctga
     6841 caaccacact tgagtccatg accgaggatc tgaacctgga ctcacccctg accccggaat
     6901 tgaacgagat tctggatacc ttcctgaacg acgagtgcct cttgcatgcc atgcatatca
     6961 gcacaggact gtccatcttc gacacatctc tgttttgagc ttggaatgga tcttcgatcc
     7021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     7081 gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     7141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     7201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     7261 tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc ctcgtgatac
     7321 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7381 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7441 atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga ctcgcgctgt
     7501 caggagacta gagccaagct gatctccttt gccccggaga tcaccatgga cgactttctc
     7561 tatctctacg atctaggaag aaagttcgac ggagaaggtg acgataccat gttcaccacc
     7621 gataatgaga agattagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga
     7681 gaggcctacg cggcaggtct gatcaagacg atctacccga gtaataatct ccaggagatc
     7741 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag
     7801 aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
     7861 ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaagaa agtagttcct
     7921 actgaatcaa aggccatgga gtcaaaaatt cagatcgagg atctaacaga actcgccgtg
     7981 aagactggcg aacagttcat acagagtctt ttacgactca atgacaagaa gaaaatcttc
     8041 gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     8101 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     8161 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     8221 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcccc tgccgacagt
     8281 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     8341 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     8401 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     8461 actccggtat ttttacaaca attaccacaa caaaacaaac aacaaacaac attacaattt
     8521 actattctag tcgaaatgcc caagaagaag aggaaggtgg acaagaagta ctccattggg
     8581 ctcgctatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg
     8641 agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt
     8701 ggcgccctcc tgttcgactc cggggaaacg gccgaagcca cgcggctcaa aagaacagca
     8761 cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat
     8821 gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag
     8881 gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac
     8941 catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag
     9001 gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc
     9061 ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg
     9121 gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc
     9181 aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag
     9241 ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg
     9301 acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa
     9361 gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac
     9421 ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg
     9481 aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac
     9541 caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag
     9601 gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc
     9661 caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag
     9721 ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga
     9781 agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat
     9841 ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata
     9901 ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa
     9961 tcagaagaga ctatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc
    10021 cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt
    10081 cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa
    10141 tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc
    10201 gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagattat
    10261 ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac
    10321 gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac
    10381 aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat
    10441 agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg
    10501 aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat
    10561 gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt
    10621 gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc
    10681 cagaaagcac aagtttctgg ccagggggac agtctccacg agcacatcgc taatcttgca
    10741 ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc
    10801 aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa
    10861 actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata
    10921 aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat
    10981 gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg
    11041 gacatcaatc ggctctccga ctacgacgtg gatgccatcg tgccccagtc ttttctcaaa
    11101 gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg gaagagtgat
    11161 aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac
    11221 gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg
    11281 tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc
    11341 aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa
    11401 ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtttcaga tttcagaaag
    11461 gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac
    11521 ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt
    11581 gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa
    11641 ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc
    11701 gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa
    11761 acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc
    11821 atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa
    11881 agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc
    11941 aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa
    12001 gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc
    12061 atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa
    12121 gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac
    12181 ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg
    12241 ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct caaaggatct
    12301 cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag
    12361 atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat
    12421 aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac
    12481 attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac
    12541 accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt
    12601 catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac
    12661 agcagggctg attcggatcc taaaaagaag cgaaaggtag acccgaagaa aaagaggaag
    12721 gtagatccaa agaagaagcg caaggtagaa gtgtttgaat ttgaatatct ggatgataaa
    12781 gtgctggaag aactgctgga tagcgaagaa cgcaaacgct gagcttggaa tggatcttcg
    12841 atcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
    12901 ttgcgacgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
    12961 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
    13021 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
    13081 catctatgtt actagatcgg gaattgccaa gctaattctt gaagacgaaa gggcctcgtg
    13141 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    13201 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    13261 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatggga ccgactcgcg
    13321 ctgtcatgag accggatcct gacaggatat attggcgggt aaacctaaga gaaaagagcg
    13381 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
    13441 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
    13501 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt
    13561 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
    13621 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
    13681 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
    13741 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
    13801 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
    13861 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
    13921 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
    13981 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
    14041 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
    14101 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
    14161 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
    14221 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
    14281 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
    14341 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
    14401 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
    14461 gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
    14521 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
    14581 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
    14641 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
    14701 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
    14761 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
    14821 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
    14881 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
    14941 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
    15001 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
    15061 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
    15121 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
    15181 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
    15241 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
    15301 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
    15361 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
    15421 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
    15481 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
    15541 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
    15601 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
    15661 agcggctggg ttg
//
