LOCUS       sequence-409145-       10194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LAAPGW
FEATURES             Location/Qualifiers
     source          1..10194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        5041..5540
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5076..5099)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5473..5492
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(5558..5560,5561..5563,5564..6277)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5603..5624)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5864..5883)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6211..6232
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6338..6358)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6756..6767)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6957..7590
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7719..7735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7719..7735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7732..7754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7743..7759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7767..7773,7774..7791,7792..7797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7812..7833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7950..7967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8121..8709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8201..8220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8880..9671,9672..9740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9503..9522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9741..9845)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9893..10027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9930..9949)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9984..10003
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgttcca tgtccttata tggactcatc tttgcctatt
     2221 gcgacacaca ctcaatgaac acctactacg cgctgcaaag agccccgcag gcctgaggtg
     2281 cccccacctc accactcttc ctatttttgt gtaaaaatcc agcttcttgt caccacctcc
     2341 aaggaggggg aggaggagga aggcaggttc ctctaggctg agccgaatgc ccctctgtgg
     2401 tcccacgcca ctgatcgctg catgcccacc acctgggtac acacagtctg tgattcccgg
     2461 agcagaacgg accctgccca cccggtcttg tgtgctactc agtggacaga cccaaggcaa
     2521 gaaagggtga caaggacagg gtcttcccag gctggctttg agttcctagc accgccccgc
     2581 ccccaatcct ctgtggcaca tggagtcttg gtccccagag tcccccagcg gcctccagat
     2641 ggtctgggag ggcagttcag ctgtggctgc gcatagcaga catacaacgg acggtgggcc
     2701 tagacccagg ctgtgtagac ccagcccccc cgccccgcag tgactaggtc acccactaac
     2761 gccccaggcc tggtcttggc tgggcgtgac tgttaccctc aaaagcaggc agctccaggg
     2821 taaaaggtgc cctgccctgt agagcccacc ttccttccca gggctgcggc tgggtaggtt
     2881 tgtagccttc atcacgggcc acctccagcc actggaccgc tggcccctgc cctgtcctgg
     2941 ggagtgtggt cctgcgactt ctaagtggcc gcaagccacc tgactccccc aacaccacac
     3001 tctacctctc aagcccaggt ctctccctag tgacccaccc agcacattta gctagctgag
     3061 ccccacagcc agaggtcctc aggccctgct ttcagggcag ttgctctgaa gtcggcaagg
     3121 gggagtgact gcctggccac tccatgccct ccaagagctc cttctgcagg agcgtacaga
     3181 acccagggcc ttggcacccg tgcagaccct ggcccacccc acctgggcgc tcagtgccca
     3241 agagatgtcc acaactagga tgtcccgcgg tgggtggggg gccagagaga cgggcaggcc
     3301 gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg gcgcgggggc
     3361 cacggcgcgc gcccccagcc ccccggccca gcaccccaag gcggccaacg ccaaaactct
     3421 ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag
     3481 agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa
     3541 tcagcgtgcg ccgttccgaa agttgccttt tatggctcga acggccgcgg cggcgcccta
     3601 taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag
     3661 agcctcgcct ttgccgatcc gccgcccgtc caggctcgca tctctccttc acgcgcccgc
     3721 cgccttacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     3781 ggtgcctcct gaactacgtc cgccgtctag gtaagtttag agctcaggtc gagaccgggc
     3841 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     3901 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatca
     3961 gcgcttcgac tagggataac agggtaattg tttgaatgag gcttcagtac tttacagaat
     4021 cgttgcctgc acatcttgga aacacttgct gggattactt cgacttctta acccaacaga
     4081 aggctcgaga aggtatattg ctgttgacag gatccacatt aacaacttta agtccagcaa
     4141 tttgagttaa gggtgttgct ctcaatgatt tcactaatgg ttcaattttt aatttctttt
     4201 cttctggttc aaaattcaag tttcaagtga aagtgtaata tgcacccatt tctttcaata
     4261 aatcttctaa atagtccact aatgttttat tttgtttttt acaaaatcaa gcagcctctg
     4321 ctatcaatat agaaccttgt attccatctt tatctctagc tgagtcatca attacatatc
     4381 cataactttc ttcataagca aaaacaaaat ttaatccgtt atcttcttct ttagcaattt
     4441 ctctacccat tcatttcaat ccagttaaag tttttacaat cttaactcca tatttttcct
     4501 gagcgattct atcacccaaa tcacttgtta caaaacttga atatagagcc ggattttttg
     4561 gaatgctatt taagcgtttt agatttgata attttcaatc aattaaaatt ggtcctgttt
     4621 gatttccatc taatcttaca aaatgaccat catgttttat tgccattcca aatctgtcag
     4681 catctgggtc attcataata ataatatctg catcatgttt aataccatat tcaagcggta
     4741 tttttcatgc aggatcaaat tctggatttg gatttacaac atttttcaat gtttcatctt
     4801 caaatgcacg atcttcaccc tcaataacgt tatatcctga ttcacgtaat ctttttgggg
     4861 taaatttagt tcctgttcca ttaactgcgc taaaaatagg gccctgcctc ggacttcaag
     4921 gggctagaat tcgagcaatt atcttgttta ctaaaactga ataccttgct atctctttga
     4981 tacattttta caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattctacc
     5041 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     5101 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     5161 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     5221 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     5281 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     5341 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     5401 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     5461 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     5521 cctcatctcc gggcctttcg acctttaatt aaccaccatg gtgagcaagg gcgaggagct
     5581 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5641 cagcgtgtct ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5701 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     5761 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5821 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5881 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5941 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6001 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga acttcaagat
     6061 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6121 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6181 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6241 cgggatcact ctcggcatgg acgagctgta caagtaagtt taaacatcaa cctctggatt
     6301 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6361 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6421 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6481 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6541 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6601 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6661 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     6721 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6781 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6841 cgagtcggat ctccctttgg gccgcctccc cgcctggcct gcaggggtac ctttaagacc
     6901 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
     6961 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     7021 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     7081 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     7141 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7201 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7261 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     7321 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     7381 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7441 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7501 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7561 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7621 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
     7681 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7741 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7801 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7861 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7921 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7981 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8041 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8101 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8281 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8341 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8401 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8461 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8521 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8581 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8641 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8701 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8761 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8821 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8881 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8941 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9001 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9061 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9121 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9181 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9241 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9301 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9361 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9421 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9481 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9541 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9601 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9661 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9721 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9781 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9841 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
     9901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10021 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10081 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10141 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
