LOCUS       sequence-409146-        9541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with E2-Crimson reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LEAPEW
FEATURES             Location/Qualifiers
     source          1..9541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2185..2396
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2397..3339
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3296..3316
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4430..4929
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4465..4488)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4862..4881
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4947..5624
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(5685..5705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6103..6114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6304..6937
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7066..7082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7066..7082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7079..7101)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7090..7106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7114..7120,7121..7138,7139..7144))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7159..7180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7297..7314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7468..8056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7548..7567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8227..9018,9019..9087))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8850..8869
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9088..9192)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9240..9374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9277..9296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9331..9350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgggcag agcgcacatc gcccacagtc cccgagaagt
     2221 tggggggagg ggtcggcaat tgaaccggag cctagagaag gtggcgcggg gtaaactggg
     2281 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     2341 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     2401 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     2461 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     2521 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     2581 gaggcctggc ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     2641 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     2701 tttttctggc aagatagtct tgtaaatgcg ggccaagatc ggcacactgg tatttcggtt
     2761 tttggggccg cgggcggcga cggggccggt gcgtcccagc gcacatgttc ggcgaggcgg
     2821 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     2881 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     2941 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     3001 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     3061 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     3121 cacctcgatt agttctcgcg cttttggagt acgtcgtctt taggttgggg ggaggggttt
     3181 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     3241 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     3301 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cgcttcgact
     3361 agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca
     3421 catcttggaa acacttgctg ggattacttc gacttcttaa cccaacagaa ggctcgagaa
     3481 ggtatattgc tgttgacagg atccacatta acaactttaa gtccagcaat ttgagttaag
     3541 ggtgttgctc tcaatgattt cactaatggt tcaattttta atttcttttc ttctggttca
     3601 aaattcaagt ttcaagtgaa agtgtaatat gcacccattt ctttcaataa atcttctaaa
     3661 tagtccacta atgttttatt ttgtttttta caaaatcaag cagcctctgc tatcaatata
     3721 gaaccttgta ttccatcttt atctctagct gagtcatcaa ttacatatcc ataactttct
     3781 tcataagcaa aaacaaaatt taatccgtta tcttcttctt tagcaatttc tctacccatt
     3841 catttcaatc cagttaaagt ttttacaatc ttaactccat atttttcctg agcgattcta
     3901 tcacccaaat cacttgttac aaaacttgaa tatagagccg gattttttgg aatgctattt
     3961 aagcgtttta gatttgataa ttttcaatca attaaaattg gtcctgtttg atttccatct
     4021 aatcttacaa aatgaccatc atgttttatt gccattccaa atctgtcagc atctgggtca
     4081 ttcataataa taatatctgc atcatgttta ataccatatt caagcggtat ttttcatgca
     4141 ggatcaaatt ctggatttgg atttacaaca tttttcaatg tttcatcttc aaatgcacga
     4201 tcttcaccct caataacgtt atatcctgat tcacgtaatc tttttggggt aaatttagtt
     4261 cctgttccat taactgcgct aaaaataggg ccctgcctcg gacttcaagg ggctagaatt
     4321 cgagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac
     4381 aaagctgaat taaaatggta taaattaaat cacttttttc aattctaccg ggtaggggag
     4441 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     4501 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     4561 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     4621 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     4681 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     4741 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     4801 ggcgggctca ggggcgggct caggggcngg gcgggcgccc gaaggtcctc cggaggcccg
     4861 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     4921 ggcctttcga cctttaatta accaccatgg atagcactga gaacgtcatc aagcccttca
     4981 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     5041 tgggcgaggg caagccctac gagggcaccc agaccgccaa gctgcaagtg accaagggcg
     5101 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt cttctacggc tccaaggcgt
     5161 acatcaagca ccccgccgac atccccgact acctcaagca gtccttcccc gagggcttca
     5221 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcat
     5281 ccctgcaaga cggcaccctc atctaccacg tcaagttcat cggcgtgaac ttcccctccg
     5341 acggccccgt aatgcagaag aaaactcttg gctgggagcc ctccactgag cgcaactacc
     5401 cccgcgacgg cgtgctgaag ggcgagaacc acatggcgct gaagctgaag ggcggcggcc
     5461 actacctgtg tgagttcaag tccatctaca tggctaagaa gcccgtgaag ctgcccggct
     5521 accactacgt ggactacaag ctcgacatca cctcccacaa cgaggactac accgtggtgg
     5581 agcagtacga acgcgccgag gcccgccacc acctgttcca gtaggtttaa acatcaacct
     5641 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5701 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5761 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5821 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5881 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5941 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6001 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6061 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6121 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6181 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggcctgca ggggtacctt
     6241 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     6301 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6361 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     6421 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     6481 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     6541 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     6601 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     6661 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6721 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6781 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6841 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6901 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6961 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     7021 tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7081 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7141 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7201 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7261 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7441 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8161 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8221 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8281 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8341 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8401 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8461 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8521 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8581 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8641 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8701 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8761 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8821 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8881 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8941 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9001 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9061 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9121 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9181 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
     9241 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9301 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9361 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     9421 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
     9481 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
     9541 t
//
