LOCUS       sequence-409155-       15760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 RNAP circuit variant T15 on ultra broad host range vector 
            (RSF1010 and pAMB1 origins) chlorR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15760)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 15760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pBroad-T7RNAP-chlorR
FEATURES             Location/Qualifiers
     source          1..15760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1112..1130)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1113..1130)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     oriT            1380..1488
                     /note="incP origin of transfer"
     rep_origin      1507..1895
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(1910..1956)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5247..5304
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5328..5345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5486..5880
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            6222..6309
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             7548..8519
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             9030..9869
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             9856..10707
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             11154..11813
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11220..11239)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        15505..15523
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(15551..15553,15554..15760,1..504)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ttcagtcgtt acccggatca catgaaacgc cacgactttt tcaagtccgc gatgccggaa
       61 ggttatgtcc aagaacgtac catctcattt aaagatgacg gcacctacaa aacgcgcgcc
      121 gaagtgaaat tcgaaggtga tacgctggtt aaccgtattg aactgaaagg catcgatttt
      181 aaggaagacg gtaatattct gggccataaa ctggaatata acttcaattc gcacaacgtg
      241 tacatcaccg cagataagca gaagaacggt atcaaggcta acttcaagat ccgccataat
      301 gtggaagatg gcagcgttca actggccgac cactatcagc aaaacacccc gattggtgat
      361 ggcccggtcc tgctgccgga caatcattac ctgagcacgc agtctgtgct gagtaaagat
      421 ccgaacgaaa agcgtgacca catggtcctg ctggaattcg tgaccgcggc cggcatcacg
      481 cacggtatgg acgaactgta taaaggctca gatatatcgg gcggtatggt ttttactctg
      541 gaagattttg ttggcgattg gcgtcagacc gcgggttata atttggatca agtcctggaa
      601 cagggtggcg taagctctct gttccagaac ctgggtgtga gcgtgacgcc gattcagcgc
      661 atcgttctgt ccggcgagaa cggtctgaaa attgatattc atgtgatcat cccgtacgaa
      721 ggcctgagcg gtgaccaaat gggtcaaatc gagaaaatct ttaaagtcgt ctacccagtt
      781 gacgatcacc acttcaaggt tatcttgcat tacggtacgc tggtgattga tggtgtgacc
      841 ccgaatatga ttgactattt cggccgtccg tatgaaggca ttgccgtttt tgacggtaaa
      901 aagatcaccg tcaccggtac cctgtggaat ggcaataaga ttattgacga gcgtctgatt
      961 aacccggacg gcagcctgct gttccgcgtg accatcaacg gtgtcacggg ttggcgtctg
     1021 tgcgagcgca tcctggcata accccaactg gggtaacctc agagttctct cagttgggga
     1081 gaccggggac ttatcatcca acctgttact gtctatagtg tcacctaaat taatcgattt
     1141 aatagggata acagggtaat tacatacgtg ttccggcaaa cattggtaaa ccgatacaat
     1201 taaaggctcc ttttggagcc tttttttttt ggacgaccct tgtccttttc cgctgcataa
     1261 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     1321 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     1381 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     1441 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggccgg
     1501 cgcgccgatc tgaagatcag cagttcaacc tgttgatagt acgtactaag ctctcatgtt
     1561 tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga actaaaccct
     1621 catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt tcacgtacta
     1681 agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag cctctaaggt
     1741 tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagccttta aggtttaacg
     1801 gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc tctcaaagca
     1861 attttgagtg acacaggaac acttaacggc tgacatgggg cgcgcccaga taaaacgaaa
     1921 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctgttatcct gcatcggaac
     1981 cctcgagtag ggataacagg gtaattctag aagatcgcgc ttagaatcgc tttaggaaac
     2041 acgatccagt ccaataatcg tcgataaaaa cttttgaaaa aggttggtga aattacctac
     2101 ttttggaata atcacaaatc acaagtgatt aatcacaaat cacaagtgat taatcacttg
     2161 tttattaaga tattaaaagc tataatttaa ataaagcgtg aattttatta cacaaaaaga
     2221 ggggggagaa acttggaact agcatttaga gaaagcttaa aaaagatgag aggtaccaaa
     2281 tcaaaagaaa aattctccca agaattagaa atgagtagat caaattattc acgaatagaa
     2341 tcatgaaaat cagatccaac cataaaaaca ctagaacaaa ttgcaaagtt aactaactca
     2401 acgctagtag tggatttaat cccaaatgag ccaacagaac cagaaccaga aacagaatca
     2461 gaacaagtaa cattggattt agaaatggaa gaagaaaaaa gcaatgactt cgtgtgaata
     2521 atgcacgaaa tcgttgctta ttttttttta aaagcggtat actagatata acgaaacaac
     2581 gaactgaata gaaacgaaaa aagagccatg acacatttat aaaatgtttg acgacatttt
     2641 ataaatgcat agcccgataa gattgccaaa ccaacgctta tcagttagtc agatgaactc
     2701 ttccctcgta agaagttatt taattaactt tgtttgaaga cggtatataa ccgtactatc
     2761 attatatagg gaaatcagag agttttcaag tatctaagct actgaattta agaattgtta
     2821 agcaatcaat cggaaatcgt ttgattgctt tttttgtatt catttataga aggtggagtt
     2881 tgtatgaatc atgatgaatg taaaacttat ataaaaaata gtttattgga gataagaaaa
     2941 ttagcaaata tctatacact agaaacgttt aagaaagagt tagaaaagag aaatatctac
     3001 ttagaaacaa aatcagataa gtatttttct tcggaggggg aagattatat atataagtta
     3061 atagaaaata acaaaataat ttattcgatt agtggaaaaa aattgactta taaaggaaaa
     3121 aaatcttttt caaaacatgc aatattgaaa cagttgaatg aaaaagcaaa ccaagttaat
     3181 taaacaacct attttatagg atttatagga aaggagaaca gctgaatgaa tatccctttt
     3241 gttgtagaaa ctgtgcttca tgacggcttg ttaaagtaca aatttaaaaa tagtaaaatt
     3301 cgctcaatca ctaccaagcc aggtaaaagc aaaggggcta tttttgcgta tcgctcaaaa
     3361 tcaagcatga ttggcggtcg tggtgttgtt ctgacttccg aggaagcgat tcaagaaaat
     3421 caagatacat ttacacattg gacacccaac gtttatcgtt atggaacgta tgcagacgaa
     3481 aaccgttcat acacgaaagg acattctgaa aacaatttaa gacaaatcaa taccttcttt
     3541 attgattttg atattcacac ggcaaaagaa actatttcag caagcgatat tttaacaacc
     3601 gctattgatt taggttttat gcctactatg attatcaaat ctgataaagg ttatcaagca
     3661 tattttgttt tagaaacgcc agtctatgtg acttcaaaat cagaatttaa atctgtcaaa
     3721 gcagccaaaa taatttcgca aaatatccga gaatattttg gaaagtcttt gccagttgat
     3781 ctaacgtgta atcattttgg tattgctcgc ataccaagaa cggacaatgt agaatttttt
     3841 gatcctaatt accgttattc tttcaaagaa tggcaagatt ggtctttcaa acaaacagat
     3901 aataagggct ttactcgttc aagtctaacg gttttaagcg gtacagaagg caaaaaacaa
     3961 gtagatgaac cctggtttaa tctcttattg cacgaaacga aattttcagg agaaaagggt
     4021 ttaatagggc gtaataacgt catgtttacc ctctctttag cctactttag ttcaggctat
     4081 tcaatcgaaa cgtgcgaata taatatgttt gagtttaata atcgattaga tcaaccctta
     4141 gaagaaaaag aagtaatcaa aattgttaga agtgcctatt cagaaaacta tcaaggggct
     4201 aatagggaat acattaccat tctttgcaaa gcttgggtat caagtgattt aaccagtaaa
     4261 gatttatttg tccgtcaagg gtggtttaaa ttcaagaaaa aaagaagcga acgtcaacgt
     4321 gttcatttgt cagaatggaa agaagattta atggcttata ttagcgaaaa aagcgatgta
     4381 tacaagcctt atttagtgac gaccaaaaaa gagattagag aagtgctagg cattcctgaa
     4441 cggacattag ataaattgct gaaggtactg aaggcgaatc aggaaatttt ctttaagatt
     4501 aaaccaggaa gaaatggtgg cattcaactt gctagtgtta aatcattgtt gctatcgatc
     4561 attaaagtaa aaaaagaaga aaaagaaagc tatataaagg cgctgacaaa ttcttttgac
     4621 ttagagcata cattcattca agagacttta aacaagctag cagaacgccc taaaacggac
     4681 acacaactcg atttgtttag ctatgataca ggctgaaaat aaaacccgca ctatgccatt
     4741 acatttatat ctatgatacg tgtttgtttt ttctttgctg tttagcgaat gattagcaga
     4801 aatatacaga gtaagatttt aattaattat tagggggaga aggagagagt agcccgaaaa
     4861 cttttagttg gcttggactg aacgaagtga gggaaaggct actaaaacgt cgaggggcag
     4921 tgagagcgaa gcgaacactt gattttttaa ttttctatct tttataggtc attagagtat
     4981 acttatttgt cctataaact atttagcagc ataatagatt tattgaatag gtcatttaag
     5041 ttgagcatat tagaggagga aaatcttgga gaaatatttg aagaacccga ttacatggat
     5101 tggattagtt cttgtggtta cgtggttttt aactaaaagt agtgaatttt tgatttttgg
     5161 tgtgtgtgtc ttgttgttag tatttgctag tcaaagtgat taagatctgg catccgctta
     5221 cagacaagct gtgagaagac aagggatccg gcaaaaaagg gcaaggtgtc accaccctgc
     5281 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
     5341 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc
     5401 aattgagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg ccagccagtg
     5461 cccgtcactc ccgtactaac tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa
     5521 taactgtcac gaacccctgc aataactgtc acgcccccaa acctgcaaac ccagcagggg
     5581 cgggggctgg cggggtgttg gaaaaatcca tccatgatta tctaagaata atccactagg
     5641 cgcggttatc agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc cggccagagg
     5701 ccggatagct ggtctattcg ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg
     5761 gggaaaggcg ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat acgctggagc
     5821 gcttttagcc gctttagcgg cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc
     5881 ccgcagggcc tgtctcggtc gatcattcag cccggctcat ccttctggcg tggcggcaga
     5941 ccgaacaagg cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc catgagccga
     6001 tcctccggcc actcgctgct gttcaccttg gccaaaatca tggcccccac cagcaccttg
     6061 cgccttgttt cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg
     6121 gcattgattc gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc cttgttgctc
     6181 cccttaacca tcttgacacc ccattgttaa tgtgctgtct cgtaggctat catggaggca
     6241 cagcggcggc aatcccgacc ctactttgta ggggagggcg cacttaccgg tttctcttcg
     6301 agaaactggc ctaacggcca cccttcgggc ggtgcgctct ccgagggcca ttgcatggag
     6361 ccgaaaagca aaagcaacag cgaggcagca tggcgattta tcaccttacg gcgaaaaccg
     6421 gcagcaggtc gggcggccaa tcggccaggg ccaaggccga cttcatccag cgcgaaggca
     6481 agtatgcccg cgacatggat gaagtcttgc acgccgaatc cgggcacatg ccggagttcg
     6541 tcgagcggcc cgccgactac tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc
     6601 tgttcaagga ggtcgaattt gccctgccgg tcgagctgac cctcgaccag cagaaggcgc
     6661 tggcgtccga gttcgcccag cacctgaccg gtgccgagcg cctgccgtat acgctggcca
     6721 tccatgccgg tggcggcgag aacccgcact gccacctgat gatctccgag cggatcaatg
     6781 acggcatcga gcggcccgcc gctcagtggt tcaagcggta caacggcaag accccggaga
     6841 agggcggggc acagaagaac gaagcgctca agcccaaggc atggcttgag cagacccgcg
     6901 aggcatgggc cgaccatgcc aaccgggcat tagagcgggc tggccacgac gcccgcattg
     6961 accacagaac acttgaggcg cagggcatcg agcgcctgcc cggtgttcac ctggggccga
     7021 acgtggtgga gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca
     7081 tcgacaccgc caacgcccag atcatcgact tacaggaata ccgggaggca atagaccatg
     7141 aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg agttagcgga gcagatcgaa
     7201 ccgctggccc agagcatggc gacactggcc gacgaagccc ggcaggtcat gagccagacc
     7261 cagcaggcca gcgaggcgca ggcggcggag tggctgaaag cccagcgcca gacaggggcg
     7321 gcatgggtgg agctggccaa agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg
     7381 cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc tatggctaac cgtgatgctg
     7441 gcttccatga tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg
     7501 ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca
     7561 ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc ttcgatatcg
     7621 gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc gccgaagtgc
     7681 tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg tatatcaggc
     7741 ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag tttgacctgg
     7801 atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc ccgaagaact
     7861 atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg cagattgccc
     7921 ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc cactatggcc
     7981 gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgctggt tatcagccgt
     8041 gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc
     8101 aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc aggctggcca
     8161 gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg gacgagtacc
     8221 gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag tgcgacttta
     8281 tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc aaggccatgg
     8341 ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat tacatcgagc
     8401 gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc gagctggcac
     8461 gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc agcatgtagt
     8521 gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa gggggtttta
     8581 tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac aagggctatt
     8641 ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc ttttcagtcg
     8701 ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc gaacacaagc
     8761 ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga caagcagacc
     8821 ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga gcgcatgaag
     8881 gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga ggcgctgcgc
     8941 gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa
     9001 ccaccaactg cctgcaaagg aggcaatcaa tggctaccca taagcctatc aatattctgg
     9061 aggcgttcgc agcagcgccg ccaccgctgg actacgtttt gcccaacatg gtggccggta
     9121 cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc catgctggcc ctgcaactgg
     9181 ccgcacagat tgcaggcggg ccggatctgc tggaggtggg cgaactgccc accggcccgg
     9241 tgatctacct gcccgccgaa gatccgccca ccgccattca tcaccgcctg cacgcccttg
     9301 gggcgcacct cagcgccgag gaacggcaag ccgtggctga cggcctgctg atccagccgc
     9361 tgatcggcag cctgcccaac atcatggccc cggagtggtt cgacggcctc aagcgcgccg
     9421 ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg gttccacatc gaggaagaaa
     9481 acgccagcgg ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc gccgataccg
     9541 ggtgctctat cgtgttcctg caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg
     9601 accagcagca ggccagccgg ggcagctcgg tactggtcga taacatccgc tggcagtcct
     9661 acctgtcgag catgaccagc gccgaggccg aggaatgggg tgtggacgac gaccagcgcc
     9721 ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt
     9781 ggttcaggcg gcatgacggc ggggtgctca agcccgccgt gctggagagg cagcgcaaga
     9841 gcaagggggt gccccgtggt gaagcctaag aacaagcaca gcctcagcca cgtccggcac
     9901 gacccggcgc actgtctggc ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag
     9961 cgcagcaagc tggacgtgac gtatgactac ggcgacggca agcggatcga gttcagcggc
    10021 ccggagccgc tgggcgctga tgatctgcgc atcctgcaag ggctggtggc catggctggg
    10081 cctaatggcc tagtgcttgg cccggaaccc aagaccgaag gcggacggca gctccggctg
    10141 ttcctggaac ccaagtggga ggccgtcacc gctgatgcca tggtggtcaa aggtagctat
    10201 cgggcgctgg caaaggaaat cggggcagag gtcgatagtg gtggggcgct caagcacata
    10261 caggactgca tcgagcgcct ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg
    10321 caggggtttc ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg
    10381 gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc
    10441 atggacgagg tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt
    10501 ggctggatcg accccggcaa aaccggcaag gcttccatag ataccttgtg cggctatgtc
    10561 tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg
    10621 ttgccggagc tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg caagtacgac
    10681 atcacccggc ccaaggcggc aggctgaccc cccccactct attgtaaaca agacattttt
    10741 atcttttata ttcaatggct tattttcctg ctaattggta ataccatggt acgagctcga
    10801 catggggcgc gcccagtaac aggttggatg ataagtcccc ggtctagcac ccattagttc
    10861 aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat
    10921 attgactttt aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctatttaaa
    10981 tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
    11041 ataaaactgt ctgcttacat aaacagtaat tacttttgtt agatatattt ttttgtgtaa
    11101 ttttgtaatc gttatgcggc agtaaaagga tctattataa ggaggcactc accatggaga
    11161 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
    11221 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
    11281 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
    11341 ttgcccgcct gatgaatgct catccggaat tacgtatggc aatgaaagac ggtgagctgg
    11401 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
    11461 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
    11521 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
    11581 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
    11641 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
    11701 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
    11761 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggaggagcg taatcagaat
    11821 tggttaattg gttgtaacac tggtctatca ttgataggta taaattaata cgactcacta
    11881 atactgaacc tatcagtgat agataccggt gataccagca tcgtcttgat gcccttggca
    11941 gcaccctgct aaggaggcaa caagatgaac acgattaaca tcgctaagaa cgacttctct
    12001 gacatcgaac tggctgctat cccgttcaac actctggctg accattacgg tgagcgtttg
    12061 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc
    12121 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct
    12181 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg
    12241 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa
    12301 gccgtagcgt acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca
    12361 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt
    12421 cgtatccgtg accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag
    12481 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct
    12541 aagggtctac tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta
    12601 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt gcaccgccaa
    12661 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag
    12721 gctatcgcaa cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta
    12781 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt
    12841 cctctggcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac
    12901 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag
    12961 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc
    13021 cctgcgattg agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag
    13081 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag
    13141 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag
    13201 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc
    13261 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc
    13321 agtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag
    13381 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc
    13441 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt
    13501 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt
    13561 ccgctggcgt ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat
    13621 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg
    13681 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac
    13741 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc
    13801 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt
    13861 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa
    13921 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag
    13981 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg
    14041 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat
    14101 aagaagactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt
    14161 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc
    14221 ggtcagttcc gcttgcagcc taccattaac accaacaaag atagcgagat tgatgcacac
    14281 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt
    14341 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac
    14401 tccttcggta ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg
    14461 gttgacacat atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag
    14521 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc
    14581 cgtgacatct tggagtcgga cttcgcgttc gcgtaaaagc ttgatatcga attcctgcag
    14641 ccccggggat cccatggtac gcgtgctagt aatacgactc actaatactg aatccaaacc
    14701 ctcgttaggg gagcgtctaa ttttaggaga tccaaaatgt caaggctgga taaatcaaaa
    14761 gtaatcaata gcgcgctgga actgctgaac gaggtcggca tcgaaggtct gaccacccgc
    14821 aagctggcgc aaaaactggg cgtcgaacaa ccgacgctgt actggcacgt aaaaaataag
    14881 cgtgcgctgc tggacgcact ggcaattgaa atgctggatc gtcaccacac ccacttctgt
    14941 ccgctggagg gtgaatcatg gcaagatttc cttcgcaaca acgcgaagtc atttcgctgc
    15001 gcgctgctga gccaccgcga tggagcaaaa gttcatctgg gcacccgccc aacggagaaa
    15061 caatatgaaa cgctggaaaa ccagcttgcc ttcctgtgcc agcagggttt cagccttgag
    15121 aacgcgctgt acgcgctgag cgccgtaggt cacttcaccc tgggctgtgt tctggaagac
    15181 caagaacatc aagtagcaaa agaagagcga gaaaccccta cgaccgattc gatgccgccg
    15241 ctgctgcgtc aggcgattga actgttcgat caccagggcg cggaaccggc attcctgttt
    15301 ggtctggaac ttattatatg cggcctagaa aaacaactga agtgcgaaag cggtagctaa
    15361 cgccgaaaac cccgcttcgg cggggttttg ccgcataaca gggtaatccc caactggggt
    15421 aacctgtgag ttctctcagt tggggaaaaa aaaaaccccg cccctgacag ggcggggttt
    15481 tttttttttc gccgcgttgg ctagtaatac gactcactat agggagactt aagtataagg
    15541 aggaaaaaat atgagcaagg gcgaagaact gtttacgggc gtggtgccga ttctggtgga
    15601 actggatggt gatgtcaatg gtcacaaatt cagcgtgcgc ggcgaaggtg aaggcgatgc
    15661 aaccaatggt aaactgacgc tgaagtttat ttgcaccacg ggtaaactgc cggttccgtg
    15721 gccgaccctg gtcaccacgc tgacgtatgg tgttcagtgt
//
