LOCUS       sequence-409157-       15884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 RNAP circuit variant T15 on ultra broad host range vector 
            (RSF1010 and pAMB1 origins) apraA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15884)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 15884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pBroad-T7RNAP-apraR
FEATURES             Location/Qualifiers
     source          1..15884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1260..1278)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1261..1278)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     oriT            1528..1636
                     /note="incP origin of transfer"
     rep_origin      1655..2043
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(2058..2104)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5395..5452
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5476..5493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5634..6028
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            6370..6457
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             7696..8667
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             9178..10017
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             10004..10855
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     promoter        15777..15795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgtcaatggt cacaaattca gcgtgcgcgg cgaaggtgaa ggcgatgcaa ccaatggtaa
       61 actgacgctg aagtttattt gcaccacggg taaactgccg gttccgtggc cgaccctggt
      121 caccacgctg acgtatggtg ttcagtgttt cagtcgttac ccggatcaca tgaaacgcca
      181 cgactttttc aagtccgcga tgccggaagg ttatgtccaa gaacgtacca tctcatttaa
      241 agatgacggc acctacaaaa cgcgcgccga agtgaaattc gaaggtgata cgctggttaa
      301 ccgtattgaa ctgaaaggca tcgattttaa ggaagacggt aatattctgg gccataaact
      361 ggaatataac ttcaattcgc acaacgtgta catcaccgca gataagcaga agaacggtat
      421 caaggctaac ttcaagatcc gccataatgt ggaagatggc agcgttcaac tggccgacca
      481 ctatcagcaa aacaccccga ttggtgatgg cccggtcctg ctgccggaca atcattacct
      541 gagcacgcag tctgtgctga gtaaagatcc gaacgaaaag cgtgaccaca tggtcctgct
      601 ggaattcgtg accgcggccg gcatcacgca cggtatggac gaactgtata aaggctcaga
      661 tatatcgggc ggtatggttt ttactctgga agattttgtt ggcgattggc gtcagaccgc
      721 gggttataat ttggatcaag tcctggaaca gggtggcgta agctctctgt tccagaacct
      781 gggtgtgagc gtgacgccga ttcagcgcat cgttctgtcc ggcgagaacg gtctgaaaat
      841 tgatattcat gtgatcatcc cgtacgaagg cctgagcggt gaccaaatgg gtcaaatcga
      901 gaaaatcttt aaagtcgtct acccagttga cgatcaccac ttcaaggtta tcttgcatta
      961 cggtacgctg gtgattgatg gtgtgacccc gaatatgatt gactatttcg gccgtccgta
     1021 tgaaggcatt gccgtttttg acggtaaaaa gatcaccgtc accggtaccc tgtggaatgg
     1081 caataagatt attgacgagc gtctgattaa cccggacggc agcctgctgt tccgcgtgac
     1141 catcaacggt gtcacgggtt ggcgtctgtg cgagcgcatc ctggcataac cccaactggg
     1201 gtaacctcag agttctctca gttggggaga ccggggactt atcatccaac ctgttactgt
     1261 ctatagtgtc acctaaatta atcgatttaa tagggataac agggtaatta catacgtgtt
     1321 ccggcaaaca ttggtaaacc gatacaatta aaggctcctt ttggagcctt ttttttttgg
     1381 acgacccttg tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc
     1441 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
     1501 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
     1561 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
     1621 tctgcgaggc tggccgtagg ccggccggcg cgccgatctg aagatcagca gttcaacctg
     1681 ttgatagtac gtactaagct ctcatgtttc acgtactaag ctctcatgtt taacgtacta
     1741 agctctcatg tttaacgaac taaaccctca tggctaacgt actaagctct catggctaac
     1801 gtactaagct ctcatgtttc acgtactaag ctctcatgtt tgaacaataa aattaatata
     1861 aatcagcaac ttaaatagcc tctaaggttt taagttttat aagaaaaaaa agaatatata
     1921 aggcttttaa agcctttaag gtttaacggt tgtggacaac aagccaggga tgtaacgcac
     1981 tgagaagccc ttagagcctc tcaaagcaat tttgagtgac acaggaacac ttaacggctg
     2041 acatggggcg cgcccagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
     2101 tttgatgcct gttatcctgc atcggaaccc tcgagtaggg ataacagggt aattctagaa
     2161 gatcgcgctt agaatcgctt taggaaacac gatccagtcc aataatcgtc gataaaaact
     2221 tttgaaaaag gttggtgaaa ttacctactt ttggaataat cacaaatcac aagtgattaa
     2281 tcacaaatca caagtgatta atcacttgtt tattaagata ttaaaagcta taatttaaat
     2341 aaagcgtgaa ttttattaca caaaaagagg ggggagaaac ttggaactag catttagaga
     2401 aagcttaaaa aagatgagag gtaccaaatc aaaagaaaaa ttctcccaag aattagaaat
     2461 gagtagatca aattattcac gaatagaatc atgaaaatca gatccaacca taaaaacact
     2521 agaacaaatt gcaaagttaa ctaactcaac gctagtagtg gatttaatcc caaatgagcc
     2581 aacagaacca gaaccagaaa cagaatcaga acaagtaaca ttggatttag aaatggaaga
     2641 agaaaaaagc aatgacttcg tgtgaataat gcacgaaatc gttgcttatt tttttttaaa
     2701 agcggtatac tagatataac gaaacaacga actgaataga aacgaaaaaa gagccatgac
     2761 acatttataa aatgtttgac gacattttat aaatgcatag cccgataaga ttgccaaacc
     2821 aacgcttatc agttagtcag atgaactctt ccctcgtaag aagttattta attaactttg
     2881 tttgaagacg gtatataacc gtactatcat tatataggga aatcagagag ttttcaagta
     2941 tctaagctac tgaatttaag aattgttaag caatcaatcg gaaatcgttt gattgctttt
     3001 tttgtattca tttatagaag gtggagtttg tatgaatcat gatgaatgta aaacttatat
     3061 aaaaaatagt ttattggaga taagaaaatt agcaaatatc tatacactag aaacgtttaa
     3121 gaaagagtta gaaaagagaa atatctactt agaaacaaaa tcagataagt atttttcttc
     3181 ggagggggaa gattatatat ataagttaat agaaaataac aaaataattt attcgattag
     3241 tggaaaaaaa ttgacttata aaggaaaaaa atctttttca aaacatgcaa tattgaaaca
     3301 gttgaatgaa aaagcaaacc aagttaatta aacaacctat tttataggat ttataggaaa
     3361 ggagaacagc tgaatgaata tcccttttgt tgtagaaact gtgcttcatg acggcttgtt
     3421 aaagtacaaa tttaaaaata gtaaaattcg ctcaatcact accaagccag gtaaaagcaa
     3481 aggggctatt tttgcgtatc gctcaaaatc aagcatgatt ggcggtcgtg gtgttgttct
     3541 gacttccgag gaagcgattc aagaaaatca agatacattt acacattgga cacccaacgt
     3601 ttatcgttat ggaacgtatg cagacgaaaa ccgttcatac acgaaaggac attctgaaaa
     3661 caatttaaga caaatcaata ccttctttat tgattttgat attcacacgg caaaagaaac
     3721 tatttcagca agcgatattt taacaaccgc tattgattta ggttttatgc ctactatgat
     3781 tatcaaatct gataaaggtt atcaagcata ttttgtttta gaaacgccag tctatgtgac
     3841 ttcaaaatca gaatttaaat ctgtcaaagc agccaaaata atttcgcaaa atatccgaga
     3901 atattttgga aagtctttgc cagttgatct aacgtgtaat cattttggta ttgctcgcat
     3961 accaagaacg gacaatgtag aattttttga tcctaattac cgttattctt tcaaagaatg
     4021 gcaagattgg tctttcaaac aaacagataa taagggcttt actcgttcaa gtctaacggt
     4081 tttaagcggt acagaaggca aaaaacaagt agatgaaccc tggtttaatc tcttattgca
     4141 cgaaacgaaa ttttcaggag aaaagggttt aatagggcgt aataacgtca tgtttaccct
     4201 ctctttagcc tactttagtt caggctattc aatcgaaacg tgcgaatata atatgtttga
     4261 gtttaataat cgattagatc aacccttaga agaaaaagaa gtaatcaaaa ttgttagaag
     4321 tgcctattca gaaaactatc aaggggctaa tagggaatac attaccattc tttgcaaagc
     4381 ttgggtatca agtgatttaa ccagtaaaga tttatttgtc cgtcaagggt ggtttaaatt
     4441 caagaaaaaa agaagcgaac gtcaacgtgt tcatttgtca gaatggaaag aagatttaat
     4501 ggcttatatt agcgaaaaaa gcgatgtata caagccttat ttagtgacga ccaaaaaaga
     4561 gattagagaa gtgctaggca ttcctgaacg gacattagat aaattgctga aggtactgaa
     4621 ggcgaatcag gaaattttct ttaagattaa accaggaaga aatggtggca ttcaacttgc
     4681 tagtgttaaa tcattgttgc tatcgatcat taaagtaaaa aaagaagaaa aagaaagcta
     4741 tataaaggcg ctgacaaatt cttttgactt agagcataca ttcattcaag agactttaaa
     4801 caagctagca gaacgcccta aaacggacac acaactcgat ttgtttagct atgatacagg
     4861 ctgaaaataa aacccgcact atgccattac atttatatct atgatacgtg tttgtttttt
     4921 ctttgctgtt tagcgaatga ttagcagaaa tatacagagt aagattttaa ttaattatta
     4981 gggggagaag gagagagtag cccgaaaact tttagttggc ttggactgaa cgaagtgagg
     5041 gaaaggctac taaaacgtcg aggggcagtg agagcgaagc gaacacttga ttttttaatt
     5101 ttctatcttt tataggtcat tagagtatac ttatttgtcc tataaactat ttagcagcat
     5161 aatagattta ttgaataggt catttaagtt gagcatatta gaggaggaaa atcttggaga
     5221 aatatttgaa gaacccgatt acatggattg gattagttct tgtggttacg tggtttttaa
     5281 ctaaaagtag tgaatttttg atttttggtg tgtgtgtctt gttgttagta tttgctagtc
     5341 aaagtgatta agatctggca tccgcttaca gacaagctgt gagaagacaa gggatccggc
     5401 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
     5461 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5521 ggtatcagct cactcaaagg cggtaatcaa ttgagttctt ttaccctcag ccgaaatgcc
     5581 tgccgttgct agacattgcc agccagtgcc cgtcactccc gtactaactg tcacgaaccc
     5641 ctgcaataac tgtcacgccc ccctgcaata actgtcacga acccctgcaa taactgtcac
     5701 gcccccaaac ctgcaaaccc agcaggggcg ggggctggcg gggtgttgga aaaatccatc
     5761 catgattatc taagaataat ccactaggcg cggttatcag cgcccttgtg gggcgctgct
     5821 gcccttgccc aatatgcccg gccagaggcc ggatagctgg tctattcgct gcgctaggct
     5881 acacaccgcc ccaccgctgc gcggcagggg gaaaggcggg caaagcccgc taaaccccac
     5941 accaaacccc gcagaaatac gctggagcgc ttttagccgc tttagcggcc tttcccccta
     6001 cccgaagggt gggggcgcgt gtgcagcccc gcagggcctg tctcggtcga tcattcagcc
     6061 cggctcatcc ttctggcgtg gcggcagacc gaacaaggcg cggtcgtggt cgcgttcaag
     6121 gtacgcatcc attgccgcca tgagccgatc ctccggccac tcgctgctgt tcaccttggc
     6181 caaaatcatg gcccccacca gcaccttgcg ccttgtttcg ttcttgcgct cttgctgctg
     6241 ttcccttgcc cgcacccgct gaatttcggc attgattcgc gctcgttgtt cttcgagctt
     6301 ggccagccga tccgccgcct tgttgctccc cttaaccatc ttgacacccc attgttaatg
     6361 tgctgtctcg taggctatca tggaggcaca gcggcggcaa tcccgaccct actttgtagg
     6421 ggagggcgca cttaccggtt tctcttcgag aaactggcct aacggccacc cttcgggcgg
     6481 tgcgctctcc gagggccatt gcatggagcc gaaaagcaaa agcaacagcg aggcagcatg
     6541 gcgatttatc accttacggc gaaaaccggc agcaggtcgg gcggccaatc ggccagggcc
     6601 aaggccgact tcatccagcg cgaaggcaag tatgcccgcg acatggatga agtcttgcac
     6661 gccgaatccg ggcacatgcc ggagttcgtc gagcggcccg ccgactactg ggatgctgcc
     6721 gacctgtatg aacgcgccaa tgggcggctg ttcaaggagg tcgaatttgc cctgccggtc
     6781 gagctgaccc tcgaccagca gaaggcgctg gcgtccgagt tcgcccagca cctgaccggt
     6841 gccgagcgcc tgccgtatac gctggccatc catgccggtg gcggcgagaa cccgcactgc
     6901 cacctgatga tctccgagcg gatcaatgac ggcatcgagc ggcccgccgc tcagtggttc
     6961 aagcggtaca acggcaagac cccggagaag ggcggggcac agaagaacga agcgctcaag
     7021 cccaaggcat ggcttgagca gacccgcgag gcatgggccg accatgccaa ccgggcatta
     7081 gagcgggctg gccacgacgc ccgcattgac cacagaacac ttgaggcgca gggcatcgag
     7141 cgcctgcccg gtgttcacct ggggccgaac gtggtggaga tggaaggccg gggcatccgc
     7201 accgaccggg cagacgtggc cctgaacatc gacaccgcca acgcccagat catcgactta
     7261 caggaatacc gggaggcaat agaccatgaa cgcaatcgac agagtgaaga aatccagagg
     7321 catcaacgag ttagcggagc agatcgaacc gctggcccag agcatggcga cactggccga
     7381 cgaagcccgg caggtcatga gccagaccca gcaggccagc gaggcgcagg cggcggagtg
     7441 gctgaaagcc cagcgccaga caggggcggc atgggtggag ctggccaaag agttgcggga
     7501 ggtagccgcc gaggtgagca gcgccgcgca gagcgcccgg agcgcgtcgc gggggtggca
     7561 ctggaagcta tggctaaccg tgatgctggc ttccatgatg cctacggtgg tgctgctgat
     7621 cgcatcgttg ctcttgctcg acctgacgcc actgacaacc gaggacggct cgatctggct
     7681 gcgcttggtg gcccgatgaa gaacgacagg actttgcagg ccataggccg acagctcaag
     7741 gccatgggct gtgagcgctt cgatatcggc gtcagggacg ccaccaccgg ccagatgatg
     7801 aaccgggaat ggtcagccgc cgaagtgctc cagaacacgc catggctcaa gcggatgaat
     7861 gcccagggca atgacgtgta tatcaggccc gccgagcagg agcggcatgg tctggtgctg
     7921 gtggacgacc tcagcgagtt tgacctggat gacatgaaag ccgagggccg ggagcctgcc
     7981 ctggtagtgg aaaccagccc gaagaactat caggcatggg tcaaggtggc cgacgccgca
     8041 ggcggtgaac ttcgggggca gattgcccgg acgctggcca gcgagtacga cgccgacccg
     8101 gccagcgccg acagccgcca ctatggccgc ttggcgggct tcaccaaccg caaggacaag
     8161 cacaccaccc gcgctggtta tcagccgtgg gtgctgctgc gtgaatccaa gggcaagacc
     8221 gccaccgctg gcccggcgct ggtgcagcag gctggccagc agatcgagca ggcccagcgg
     8281 cagcaggaga aggcccgcag gctggccagc ctcgaactgc ccgagcggca gcttagccgc
     8341 caccggcgca cggcgctgga cgagtaccgc agcgagatgg ccgggctggt caagcgcttc
     8401 ggtgatgacc tcagcaagtg cgactttatc gccgcgcaga agctggccag ccggggccgc
     8461 agtgccgagg aaatcggcaa ggccatggcc gaggccagcc cagcgctggc agagcgcaag
     8521 cccggccacg aagcggatta catcgagcgc accgtcagca aggtcatggg tctgcccagc
     8581 gtccagcttg cgcgggccga gctggcacgg gcaccggcac cccgccagcg aggcatggac
     8641 aggggcgggc cagatttcag catgtagtgc ttgcgttggt actcacgcct gttatactat
     8701 gagtactcac gcacagaagg gggttttatg gaatacgaaa aaagcgcttc agggtcggtc
     8761 tacctgatca aaagtgacaa gggctattgg ttgcccggtg gctttggtta tacgtcaaac
     8821 aaggccgagg ctggccgctt ttcagtcgct gatatggcca gccttaacct tgacggctgc
     8881 accttgtcct tgttccgcga acacaagcct ttcggccccg gcaagtttct cggtgactga
     8941 tatgaaagac caaaaggaca agcagaccgg cgacctgctg gccagccctg acgctgtacg
     9001 ccaagcgcga tatgccgagc gcatgaaggc caaagggatg cgtcagcgca agttctggct
     9061 gaccgacgac gaatacgagg cgctgcgcga gtgcctggaa gaactcagag cggcgcaggg
     9121 cgggggtagt gaccccgcca gcgcctaacc accaactgcc tgcaaaggag gcaatcaatg
     9181 gctacccata agcctatcaa tattctggag gcgttcgcag cagcgccgcc accgctggac
     9241 tacgttttgc ccaacatggt ggccggtacg gtcggggcgc tggtgtcgcc cggtggtgcc
     9301 ggtaaatcca tgctggccct gcaactggcc gcacagattg caggcgggcc ggatctgctg
     9361 gaggtgggcg aactgcccac cggcccggtg atctacctgc ccgccgaaga tccgcccacc
     9421 gccattcatc accgcctgca cgcccttggg gcgcacctca gcgccgagga acggcaagcc
     9481 gtggctgacg gcctgctgat ccagccgctg atcggcagcc tgcccaacat catggccccg
     9541 gagtggttcg acggcctcaa gcgcgccgcc gagggccgcc gcctgatggt gctggacacg
     9601 ctgcgccggt tccacatcga ggaagaaaac gccagcggcc ccatggccca ggtcatcggt
     9661 cgcatggagg ccatcgccgc cgataccggg tgctctatcg tgttcctgca ccatgccagc
     9721 aagggcgcgg ccatgatggg cgcaggcgac cagcagcagg ccagccgggg cagctcggta
     9781 ctggtcgata acatccgctg gcagtcctac ctgtcgagca tgaccagcgc cgaggccgag
     9841 gaatggggtg tggacgacga ccagcgccgg ttcttcgtcc gcttcggtgt gagcaaggcc
     9901 aactatggcg caccgttcgc tgatcggtgg ttcaggcggc atgacggcgg ggtgctcaag
     9961 cccgccgtgc tggagaggca gcgcaagagc aagggggtgc cccgtggtga agcctaagaa
    10021 caagcacagc ctcagccacg tccggcacga cccggcgcac tgtctggccc ccggcctgtt
    10081 ccgtgccctc aagcggggcg agcgcaagcg cagcaagctg gacgtgacgt atgactacgg
    10141 cgacggcaag cggatcgagt tcagcggccc ggagccgctg ggcgctgatg atctgcgcat
    10201 cctgcaaggg ctggtggcca tggctgggcc taatggccta gtgcttggcc cggaacccaa
    10261 gaccgaaggc ggacggcagc tccggctgtt cctggaaccc aagtgggagg ccgtcaccgc
    10321 tgatgccatg gtggtcaaag gtagctatcg ggcgctggca aaggaaatcg gggcagaggt
    10381 cgatagtggt ggggcgctca agcacataca ggactgcatc gagcgccttt ggaaggtatc
    10441 catcatcgcc cagaatggcc gcaagcggca ggggtttcgg ctgctgtcgg agtacgccag
    10501 cgacgaggcg gacgggcgcc tgtacgtggc cctgaacccc ttgatcgcgc aggccgtcat
    10561 gggtggcggc cagcatgtgc gcatcagcat ggacgaggtg cgggcgctgg acagcgaaac
    10621 cgcccgcctg ctgcaccagc ggctgtgtgg ctggatcgac cccggcaaaa ccggcaaggc
    10681 ttccatagat accttgtgcg gctatgtctg gccgtcagag gccagtggtt cgaccatgcg
    10741 caagcgccgc cagcgggtgc gcgaggcgtt gccggagctg gtcgcgctgg gctggacggt
    10801 aaccgagttc gcggcgggca agtacgacat cacccggccc aaggcggcag gctgaccccc
    10861 cccactctat tgtaaacaag acatttttat cttttatatt caatggctta ttttcctgct
    10921 aattggtaat accatggtac gagctcgaca tggggcgcgc ccagtaacag gttggatgat
    10981 aagtccccgg tctagcaccc attagttcaa caaacgaaaa ttggataaag tgggatattt
    11041 ttaaaatata tatttatgtt acagtaatat tgacttttaa aaaaggattg attctaatga
    11101 agaaagcaga caagtaagcc tatttaaatt tgtgtctcaa aatctctgat gttacattgc
    11161 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatta
    11221 cttttgttag atatattttt ttgtgtaatt ttgtaatcgt tatgcggcag taaaaggatc
    11281 tattataagg aggcactcac catgcaatac gaatggcgaa aagccgaact catcggtcag
    11341 cttctcaacc ttggggttac ccccggcggt gtgctgctgg tccacagctc cttccgtagc
    11401 gtccggcccc tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc tgcgctgggt
    11461 ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct
    11521 gccacgtcgc ccgttacacc ggaccttgga gttgtctctg acacattctg gcgcctgcca
    11581 aatgtaaagc gcagcgccca tccatttgcc tttgcggcag cggggccaca ggcagagcag
    11641 atcatctctg atccattgcc cctgccacct cactcgcctg caagcccggt cgcccgtgtc
    11701 catgaactcg atgggcaggt acttctcctc ggcgtgggac acgatgccaa cacgacgctg
    11761 catcttgccg agttgatggc aaaggttccc tatggggtgc cgagacactg caccattctt
    11821 caggatggca agttggtacg cgtcgattat ctcgagaatg accactgctg tgagcgcttt
    11881 gccttggcgg acaggtggct caaggagaag agccttcaga aggaaggtcc agtcggtcat
    11941 gcctttgctc ggttgatccg ctcccgcgac attgtggcga cagccctggg tcaactgggc
    12001 cgagatccgt tgatcttcct gcatccgcca gaggcgggat gcgaagaatg cgatgccgct
    12061 cgccagtcga ttggctaata gggataatca gaattggtta attggttgta acactggtct
    12121 atcattgata ggtataaatt aatacgactc actaatactg aacctatcag tgatagatac
    12181 cggtgatacc agcatcgtct tgatgccctt ggcagcaccc tgctaaggag gcaacaagat
    12241 gaacacgatt aacatcgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt
    12301 caacactctg gctgaccatt acggtgagcg tttggctcgc gaacagttgg cccttgagca
    12361 tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc
    12421 tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat
    12481 gattacacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca agcacccgac
    12541 agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca ccattaagac
    12601 cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat
    12661 cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca
    12721 cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagaaagc
    12781 atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg
    12841 gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg agatgctcat
    12901 tgagtcaacc ggaatggtta gcttgcaccg ccaaaatgct ggcgtagtag gtcaagactc
    12961 tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct
    13021 ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt ggactggcat
    13081 tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag
    13141 taagaaagca ctgatgcgct acgaagacat ttacatgcct gaggtgtaca aagcgattaa
    13201 cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat
    13261 caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg aagaactccc
    13321 gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga aacgtgctgc
    13381 cgctgccgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc ttgagttcat
    13441 gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt acaacatgga
    13501 ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa
    13561 aggactgctt acgctggcga aaggtaaacc aatcagtaag gaaggttact actggctgaa
    13621 aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc gcatcaagtt
    13681 cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg agaacacttg
    13741 gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt acgctggggt
    13801 acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg ggtcttgctc
    13861 tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg cggttaactt
    13921 gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag tcaacgagat
    13981 tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga ccgatgagaa
    14041 cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg gtcaatggct
    14101 ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc
    14161 caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag ctattgattc
    14221 cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg ctaagctgat
    14281 ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc
    14341 tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga ttcttcgcaa
    14401 gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg aatacaagaa
    14461 gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttgc agcctaccat
    14521 taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta tcgctcctaa
    14581 ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg cacacgagaa
    14641 gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc cggctgacgc
    14701 tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt cttgtgatgt
    14761 actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat tggacaaaat
    14821 gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttggagt cggacttcgc
    14881 gttcgcgtaa aagcttgata tcgaattcct gcagccccgg ggatcccatg gtacgcgtgc
    14941 tagtaatacg actcactaat actgaatcca aaccctcgtt aggggagcgt ctaatttagg
    15001 agatccaaaa tgtcaaggct ggataaatca aaagtaatca atagcgcgct ggaactgctg
    15061 aacgaggtcg gcatcgaagg tctgaccacc cgcaagctgg cgcaaaaact gggcgtcgaa
    15121 caaccgacgc tgtactggca cgtaaaaaat aagcgtgcgc tgctggacgc actggcaatt
    15181 gaaatgctgg atcgtcacca cacccacttc tgtccgctgg agggtgaatc atggcaagat
    15241 ttccttcgca acaacgcgaa gtcatttcgc tgcgcgctgc tgagccaccg cgatggagca
    15301 aaagttcatc tgggcacccg cccaacggag aaacaatatg aaacgctgga aaaccagctt
    15361 gccttcctgt gccagcaggg tttcagcctt gagaacgcgc tgtacgcgct gagcgccgta
    15421 ggtcacttca ccctgggctg tgttctggaa gaccaagaac atcaagtagc aaaagaagag
    15481 cgagaaaccc ctacgaccga ttcgatgccg ccgctgctgc gtcaggcgat tgaactgttc
    15541 gatcaccagg gcgcggaacc ggcattcctg tttggtctgg aacttattat atgcggccta
    15601 gaaaaacaac tgaagtgcga aagcggtagc taacgccgaa aaccccgctt cggcggggtt
    15661 ttgccgcata acagggtaat ccccaactgg ggtaacctgt gagttctctc agttggggaa
    15721 aaaaaaaccc cgcccctgac agggcggggt tttttttttt tcgccgcgtt ggctagtaat
    15781 acgactcact atagggagac ttaagtataa ggaggaaaaa atatgaacaa gggcgaagaa
    15841 ctgtttacgg gcgtggtgcc gattctggtg gaactggatg gtga
//
