LOCUS       sequence-409188-       15451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Small molecule helper circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15451)
  AUTHORS   Wauford N, Patel A, Tordoff J, Enghuus C, Jin A, Toppen J, Kemp ML, 
            Weiss R
  TITLE     Synthetic symmetry breaking and programmable multicellular structure
            formation.
  JOURNAL   Cell Syst. 2023 Sep 20;14(9):806-818.e5. doi: 
            10.1016/j.cels.2023.08.001. Epub 2023 Sep 8.
  PUBMED    37689062
REFERENCE   2  (bases 1 to 15451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.08.001"; journalName: "Cell Syst"; date: 
            "2023-09-20- 20"; volume: "14"; issue: "9"; pages: "806-818.e5"
COMMENT     Sequence Label: aCE106
FEATURES             Location/Qualifiers
     source          1..15451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   338..372
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(408..426)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(589..608)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             600..1409
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(667..686)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1564..2152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2053..2072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2306..2323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   2354..2416
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        3276..3487
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3488..4426
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      4469..4478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4475..4849
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4902..4950
                     /note="synthetic polyadenylation signal"
     promoter        5579..5790
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5791..6729
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6803..6812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8354..8377
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    8461..8509
                     /note="synthetic polyadenylation signal"
     promoter        9146..9357
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          9358..10296
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      10342..10351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10354..10374
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    10420..10444
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10474..10623
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             10648..10668
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11098..11454
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             11473..12042
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             12583..12609
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    12671..12719
                     /note="synthetic polyadenylation signal"
     promoter        13356..13567
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          13568..14506
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    14549..14573
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             14583..15290
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(15293..15317)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    15370..15418
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gttcgcggct ccagcctaca caatcgctca agacgtgtaa tgcttttatt atatattagt
       61 cacgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
      121 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
      181 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
      241 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
      301 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
      361 atctttctag ggttaaaagc ttggcgtaat catgaattca ggaaagggcc tcgtgatacg
      421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      481 tcggggaaat gtgctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      541 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      601 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
      661 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
      721 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
      781 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
      841 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
      901 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
      961 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     1021 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     1081 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     1141 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     1201 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     1261 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     1321 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     1381 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     1441 agagcattac gctgacttga cgggacggaa gatccttttt gataatctca tgaccaaaat
     1501 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1681 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2161 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2221 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2281 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcag agcgtgccac
     2341 ctggtcgact taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgc
     2401 gtaaaattga cgcatgtgtt ttatcgatct gtatatcgag gtttatttat taatttgaat
     2461 agatattaag ttttattata tttacactta catactaata ataaattcaa caaacaattt
     2521 atttatgttt atttatttat taaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     2581 caaaactttt aaacattctc tcttttacaa aaataaactt attttgtact ttaaaaacag
     2641 tcatgttggt aaaacgacgg ccaggcagta ccggagttct gcaattcatg tgatctgcgc
     2701 ctgggagctc acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     2761 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
     2821 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
     2881 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
     2941 aaaagctagg ctgaaagagg atcttagaat gacacgcgcc tgggagctca cggggacagc
     3001 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
     3061 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
     3121 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
     3181 tgctctttga gcctgcagac acctgggggg atacggggaa aaagctggat cataactcga
     3241 cgtgcatact ttaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     3301 ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     3361 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     3421 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     3481 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     3541 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     3601 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     3661 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     3721 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     3781 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     3841 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     3901 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     3961 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     4021 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     4081 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     4141 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     4201 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     4261 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     4321 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     4381 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgg atcccaaggg
     4441 cagataagtt tgtacaaaaa agagaggtgc caccatggcc aagttgacca gtgccgttcc
     4501 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     4561 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     4621 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     4681 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     4741 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     4801 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgag cttccactgg
     4861 attgtacaat taccaactga tctagaaccg gtcatggccg caataaaata tctttatttt
     4921 cattacatct gtgtgttggt tttttgtgtg ttcgaactag atgctgtcga ccgatgcgct
     4981 tctgcaattc atgtgatctg cgcctgggag ctcacgggga cagccccccc ccaaagcccc
     5041 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     5101 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     5161 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     5221 agacacctgg ggggatacgg ggaaaaagct aggctgaaag aggatcttag aatgacacgc
     5281 gcctgggagc tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     5341 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     5401 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     5461 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     5521 gaaaaagctg gatcataact cgacgtgcat actttaattg gctccggtgc ccgtcagtgg
     5581 gcagagcgca catcgcccac agtccccgag aagttgtggg gaggggtcgg caattgaacc
     5641 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5701 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5761 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     5821 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     5881 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     5941 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     6001 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     6061 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     6121 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     6181 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     6241 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     6301 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     6361 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     6421 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     6481 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     6541 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     6601 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     6661 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     6721 ccatttcagg tggatcccaa gggcagataa gtttgtacaa aaaagagagg tatcccggta
     6781 ccctcgacac ctgctgacag gtgccaccat ggcccggacc cccagcagaa gcagcatcgg
     6841 atctctgaga agcccccaca cccacaaggc catcctgacc agcaccatcg agatcctgaa
     6901 agagtgcggc tacagcggcc tgagcatcga gtctgtggct agaagggctg gcgcctccaa
     6961 gcccaccatc taccggtggt ggacaaacaa ggccgccctg atcgccgagg tgtacgagaa
     7021 cgagagcgag caggtccgaa agttccccga cctgggcagc ttcaaggccg atctggactt
     7081 cctgctgcgg aacctgtgga aagtgtggcg ggaaaccatc tgcggcgagg ccttcagatg
     7141 cgtgatcgct gaggcccagc tggaccctgc cacactgacc cagctgaagg accagttcat
     7201 ggaacggcgg agagagatgc ccaagaaact ggtggaaaac gccatcagca acggcgagct
     7261 gcccaaggac accaaccggg aactgctgct ggatatgatc ttcggcttct gctggtacag
     7321 actgctgacc gagcagctga ccgtggaaca ggatatcgag gaattcacct ttctgctgat
     7381 caacggcgtg tgccccggca cccagagagc gttagcactt aaattagctg gtttggacat
     7441 aggcggcgcc agcacgcgtg tgcctttgta tggttttact tcgatttgtg gaagaagacc
     7501 tgagatggaa gctgctgttt cgactatacc aagattcctt caatcttcct ctggttcgat
     7561 gttagatggt cggtttgatc ctcaatccgc cgctcatttc ttcggtgttt acgacggcca
     7621 tggcggttct caggtagcga actattgtag agagaggatg catttggctt tggcggagga
     7681 gatagctaag gagaaaccga tgctctgcga tggtgatacg tggctggaga agtggaagaa
     7741 agctcttttc aactcgttcc tgagagttga ctcggagatt gagtcagttg cgccggagac
     7801 ggttgggtca acgtcggtgg ttgccgttgt tttcccgtct cacatcttcg tcgctaactg
     7861 cggtgactct agagccgttc tttgccgcgg caaaactgca cttccattat ccgttgacca
     7921 taaaccggat agagaagatg aagctgcgag gattgaagcc gcaggaggga aagtgattca
     7981 gtggaatgga gctcgtgttt tcggtgttct cgccatgtcg agatccattg gcgatagata
     8041 cttgaaacca tccatcattc ctgatccgga agtgacggct gtgaagagag taaaagaaga
     8101 tgattgtctg attttggcga gtgacggggt ttgggatgta atgacggatg aagaagcgtg
     8161 tgagatggca aggaagcgga ttctcttgtg gcacaagaaa aacgcggtgg ctggggatgc
     8221 atcgttgctc gcggatgagc ggagaaagga agggaaagat cctgcggcga tgtccgcggc
     8281 tgagtatttg tcaaagctgg cgatacagag aggaagcaaa gacaacataa gtgtggtggt
     8341 ggttgatttg aaggattaca aggacgatga cgataagtga agcttcagtg caggtgaatt
     8401 ctaccaccgc ttccactgga ttgtacaatt accaactgat ctagaaccgg tcatggccgc
     8461 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgt tcgaactaga
     8521 tgctgtcgac cgatgcgctc gcggagttct gcaattcatg tgatctgcgc ctgggagctc
     8581 acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg
     8641 ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc
     8701 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga
     8761 acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagctagg
     8821 ctgaaagagg atcttagaat gacacgcgcc tgggagctca cggggacagc ccccccccaa
     8881 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     8941 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
     9001 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     9061 gcctgcagac acctgggggg atacggggaa aaagctggat cataactcga cgtgcatact
     9121 ttaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     9181 ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     9241 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     9301 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     9361 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     9421 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     9481 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     9541 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     9601 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     9661 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     9721 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     9781 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     9841 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     9901 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     9961 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
    10021 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
    10081 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
    10141 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
    10201 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
    10261 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgg atcccaaggg cagataagtt
    10321 tgtacaaaaa agagaggtgc cgccaccatg ggccccaaga agaagaggaa ggtgggatct
    10381 ggctcgccag ggatccgtcg acttgacgcg ttgatatcaa caagtttgta caaaaaagca
    10441 ggctacaaag aggccagcgg ttccggacgg gctgacgcat tggacgattt tgatctggat
    10501 atgctgggaa gtgacgccct cgatgatttt gaccttgaca tgcttggttc ggatgccctt
    10561 gatgactttg acctcgacat gctcggcagt gacgcccttg atgatttcga cctggacatg
    10621 ctgattaact ctagaagttc cggatctccg aaaaagaaac gcaaagttgg tagccagtac
    10681 ctgcccgaca ccgacgaccg gcaccggatc gaggaaaagc ggaagcggac ctacgagaca
    10741 ttcaagagca tcatgaagaa gtcccccttc agcggcccca ccgaccctag acctccacct
    10801 agaagaatcg ccgtgcccag cagatccagc gccagcgtgc caaaacctgc cccccagcct
    10861 taccccttca ccagcagcct gagcaccatc aactacgacg agttccctac catggtgttc
    10921 cccagcggcc agatctctca ggcctctgct ctggctccag cccctcctca ggtgctgcct
    10981 caggctcctg ctcctgcacc agctccagcc atggtgtctg cactggctca ggcaccagca
    11041 cccgtgcctg tgctggctcc tggacctcca caggctgtgg ctccaccagc ccctaaacct
    11101 acacaggccg gcgagggcac actgtctgaa gctctgctgc agctgcagtt cgacgacgag
    11161 gatctgggag ccctgctggg aaacagcacc gatcctgccg tgttcaccga cctggccagc
    11221 gtggacaaca gcgagttcca gcagctgctg aaccagggca tccctgtggc ccctcacacc
    11281 accgagccca tgctgatgga ataccccgag gccatcaccc ggctcgtgac aggcgctcag
    11341 aggcctcctg atccagctcc tgcccctctg ggagcaccag gcctgcctaa tggactgctg
    11401 tctggcgacg aggacttcag ctctatcgcc gatatggatt tctcagcctt gctgggctct
    11461 ggcagcggca gccgggattc cagggaaggg atgtttttgc cgaagcctga ggccggctcc
    11521 gctattagtg acgtgtttga gggccgcgag gtgtgccagc caaaacgaat ccggccattt
    11581 catcctccag gaagtccatg ggccaaccgc ccactccccg ccagcctcgc accaacacca
    11641 accggtccag tacatgagcc agtcgggtca ctgaccccgg caccagtccc tcagccactg
    11701 gatccagcgc ccgcagtgac tcccgaggcc agtcacctgt tggaggatcc cgatgaagag
    11761 acgagccagg ctgtcaaagc ccttcgggag atggccgata ctgtgattcc ccagaaggaa
    11821 gaggctgcaa tctgtggcca aatggacctt tcccatccgc ccccaagggg ccatctggat
    11881 gagctgacaa ccacacttga gtccatgacc gaggatctga acctggactc acccctgacc
    11941 ccggaattga acgagattct ggataccttc ctgaacgacg agtgcctctt gcatgccatg
    12001 catatcagca caggactgtc catcttcgac acatctctgt ttggcgcgcc aactcaagac
    12061 gaattcaccc aactctccca atcaatcgcc gagttccaca cgtaccaact cggtaacggc
    12121 cgttgctcat ctctcctagc tcagcgaatc cacgcgccgc cggaaacagt atggtccgtg
    12181 gtgagacgtt tcgataggcc acagatttac aaacacttca tcaaaagctg taacgtgagt
    12241 gaagatttcg agatgcgagt gggatgcacg cgcgacgtga acgtgataag tggattaccg
    12301 gcgaatacgt ctcgagagag attagatctg ttggacgatg atcggagagt gactgggttt
    12361 agtataaccg gtggtgaaca taggctgagg aattataaat cggttacgac ggttcataga
    12421 tttgagaaag aagaagaaga agaaaggatc tggaccgttg ttttggaatc ttatgttgtt
    12481 gatgtaccgg aaggtaattc ggaggaagat acgagattgt ttgctgatac ggttattaga
    12541 ttgaatcttc agaaacttgc ttcgatcact gaagctatga actacccata cgatgttcca
    12601 gattacgctt gaggatccgc ttccactgga ttgtacaatt accaactgat ctagaaccgg
    12661 tcatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgt
    12721 tcgaactaga tgctgtcgac cgatgcgctt acggagttct gcaattcatg tgatctgcgc
    12781 ctgggagctc acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
    12841 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
    12901 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
    12961 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
    13021 aaaagctagg ctgaaagagg atcttagaat gacacgcgcc tgggagctca cggggacagc
    13081 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
    13141 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
    13201 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
    13261 tgctctttga gcctgcagac acctgggggg atacggggaa aaagctggat cataactcga
    13321 cgtgcatact ttaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
    13381 ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
    13441 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
    13501 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
    13561 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
    13621 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
    13681 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
    13741 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
    13801 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
    13861 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
    13921 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
    13981 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
    14041 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
    14101 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
    14161 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
    14221 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
    14281 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
    14341 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
    14401 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
    14461 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgg atcccaaggg
    14521 cagataagtt tgtacaaaaa agagaggtcc aagtttgtac aaaaaagcag gctgatgcca
    14581 ccatgtctag gctggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
    14641 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
    14701 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
    14761 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
    14821 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
    14881 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
    14941 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
    15001 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
    15061 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
    15121 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
    15181 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
    15241 tcccagccga tgcccttgac gattttgacc ttgacatgct ccccgggtaa atacccagct
    15301 ttcttgtaca aagtggtgct tccactggat tgtacaatta ccaactgatc tagaaccggt
    15361 catggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgtt
    15421 cgaactagat gctgtcgacc gatgcgctag a
//
