LOCUS       sequence-409225-        9459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Flag-Tagged human HNRNPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  TITLE     Alarcon lab plasmids
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label:  pLenti-C-Myc-DDK-IRES-Puro HNRNPR
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    34..607
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    57..609
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(201..218)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     428..447
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             609..1208
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(609..628)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1105..1125
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(1238..1271)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1327..1915
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1380..1400)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1798..1809)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1918..1934)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1919..1935)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2444..2624
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2686..3274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2766..2785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3351..4010)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3925..3944
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4011..4115)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4190..4209)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4380..4759
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4761..4959
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4977..5157
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5204..5329
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5826..6059
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6244..6288
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6586..6703
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6786..7165
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7166..7369
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7395..7414
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7395..7413
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9388..9417
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9436..9459
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtttaaacct aggcgtagcg gccgcaaatt ccgcccctct ccctcccccc cccctaacgt
       61 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
      121 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
      181 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
      241 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
      301 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
      361 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
      421 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
      481 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
      541 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
      601 atgataatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
      661 gggcagtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
      721 atccagaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
      781 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
      841 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
      901 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
      961 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     1021 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     1081 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     1141 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     1201 gtgcctgatg tacaagtagg attcgtcgag ggacctaata acttcgtata gcatacatta
     1261 tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga tatcaagctt
     1321 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1381 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     1441 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     1501 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     1561 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     1621 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     1681 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     1741 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     1801 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     1861 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     1921 taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     1981 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     2041 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     2101 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     2161 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     2221 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     2281 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     2341 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     2401 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     2461 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2521 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2581 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg
     2641 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2701 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2761 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2821 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2881 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2941 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3001 caacccggta agacacgact tatcgccact ggcagcagcc gctggtaaca ggattagcag
     3061 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3121 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3181 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3241 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3301 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga ttacgccccg
     3361 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     3421 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     3481 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     3541 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     3601 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     3661 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     3721 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     3781 attgccatac ggaactccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     3841 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     3901 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     3961 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat actcttcctt
     4021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4141 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     4201 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     4261 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     4321 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatcgcgttg
     4381 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4441 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4501 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4561 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4621 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4681 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4741 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     4801 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4861 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     4921 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
     4981 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5041 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5101 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     5161 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     5221 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     5281 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     5341 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     5401 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     5461 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     5521 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     5581 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     5641 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     5701 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     5761 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     5821 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     5881 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     5941 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     6001 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     6061 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     6121 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     6181 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     6241 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     6301 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     6361 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     6421 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     6481 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     6541 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     6601 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     6661 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     6721 cagcagagat ccagtttggt tagtaccggg cccgctctag acatgtccaa tatgaccgcc
     6781 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     6841 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     6901 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     6961 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     7021 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
     7081 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
     7141 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
     7201 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     7261 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
     7321 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     7381 ccgtcagaat tttgtaatac gactcactat agggcggccg ggaattcgtc gactggatcc
     7441 ggtaccgagg agatctgccg ccgcgatcgc atggctaatc aggtgaatgg taatgcggta
     7501 cagttaaaag aagaggaaga accaatggat acttccagtg taactcacac agaacactac
     7561 aagacactga tagaggcagg cctcccacag aaggtggcag aaagacttga tgaaatattt
     7621 cagacaggat tggtagctta tgtcgatctt gatgaaagag caattgatgc tctcagggaa
     7681 tttaatgaag aaggagctct gtctgtacta cagcagttca aggaaagtga cttatcacat
     7741 gttcagaaca aaagtgcatt tttatgtgga gttatgaaga cctacaggca gagagagaaa
     7801 caggggagca aggtgcaaga gtccacaaag ggacctgatg aagcgaagat caaggccttg
     7861 cttgagagaa ctggttatac tctggatgta accacaggac agaggaagta tggtggtcct
     7921 ccaccagaca gtgtgtactc tggcgtgcaa cctggaattg gaacggaggt atttgtaggc
     7981 aaaataccaa gggatttata tgaggatgag ttggtgcccc tttttgagaa ggccggaccc
     8041 atttgggatc tacgtcttat gatggatcca ctgtccggtc agaatagagg gtatgcattt
     8101 atcaccttct gtggaaagga agctgcacag gaagccgtga aactgtgtga cagctatgaa
     8161 attcgccctg gtaaacacct tggagtgtgc atttctgtgg caaacaacag actttttgtt
     8221 ggatccattc cgaagaataa gactaaagaa aacattttgg aagaattcag taaagtcaca
     8281 gagggtttgg tggacgttat tctctatcat caacccgatg acaaaaagaa gaatcggggg
     8341 ttctgcttcc ttgaatatga ggatcacaag tcagcagcac aagccagacg ccggctgatg
     8401 agtggaaaag taaaagtgtg gggaaatgta gttacagttg aatgggctga ccctgtggaa
     8461 gaaccagatc cagaagtcat ggctaaggta aaagttttgt ttgtgagaaa cttggctact
     8521 acggtgacag aagaaatatt ggaaaagtca ttttctgaat ttggaaaact cgaaagagta
     8581 aagaagttga aagattatgc atttgttcat tttgaagaca gaggagcagc tgttaaggct
     8641 atggatgaaa tgaatggcaa agaaatagaa ggggaagaaa ttgaaatagt cttagccaag
     8701 ccaccagaca agaaaaggaa agagcgccaa gctgctagac aggcctccag aagcactgcg
     8761 tatgaagatt attactacca ccctcctcct cgcatgccac ctccaattag aggtcggggt
     8821 cgtggtgggg ggagaggtgg atatggctac cctccagatt actacggcta tgaagattac
     8881 tatgatgatt actatggtta tgattatcac gactatcgtg gaggctatga agatccctac
     8941 tacggctatg atgatggcta tgcagtaaga ggaagaggag gaggaagggg agggcgaggt
     9001 gctccaccac caccaagggg gaggggagca ccacctccaa gaggtagagc tggctattca
     9061 cagagggggg cacctttggg accaccaaga ggctctaggg gtggcagagg gggtcctgct
     9121 caacagcaga gaggccgtgg ttcccgtgga tctcggggca atcgtggggg caatgtagga
     9181 ggcaagagaa aggcagatgg gtacaaccag cctgattcca agcgtcgtca gaccaacaac
     9241 caacagaact ggggttccca acccatcgct cagcagccgc ttcagcaagg tggtgactat
     9301 tctggtaact atggttacaa taatgacaac caggaatttt atcaggatac ttatgggcaa
     9361 cagtggaaga cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca
     9421 gcaaatgata tcctggatta caaggatgac gacgataag
//
