LOCUS       sequence-409288-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dimerization dependent fluorescent protein B anchored to cytosolic 
            face of the lysosomal membrane with human LAMP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Miner GE, Smith SY, Showalter WK, So CM, Ragusa JV, Powers AE, 
            Zanellati MC, Hsu CH, Marchan MF, Cohen S
  TITLE     Contact-FP: A Dimerization-Dependent Fluorescent Protein Toolkit for
            Visualizing Membrane Contact Site Dynamics.
  JOURNAL   Contact (Thousand Oaks). 2024 Feb 4;7:25152564241228911. doi: 
            10.1177/25152564241228911. eCollection 2024 Jan-Dec.
  PUBMED    38327561
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Contact 
            (Thousand Oaks)."; date: "2024-02-4"; volume: "7:25152564241228911. 
            doi"; pages: " 10.1177/25152564241228911. eCollection 2024 Jan-Dec"
COMMENT     Sequence Label: Lyso-B
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(116..134)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     384..403
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     578..601
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gccaccatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
       61 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      121 ctgaaggtga ccaagggcgg ccccctgccc tttgcctggg acatcctgtc cccccagatc
      181 atgtacggct ccaaagcgta cgtgaggcac cccgccgaca tccccgatta caagaagctg
      241 cccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaagacgg cggtctggtg
      301 accgttaccc aggactcctc cctgcaggac ggcacgctga tctgcaaggt gaagattcgc
      361 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      421 tccaccgaga tgctgtaccc caaggacggc gtgctgaagg gccatagcta tcaggccctg
      481 aagctgaagg acggcggcca ctacctggtg gagttcgaga ccatctacat ggccaagaag
      541 cccgtgcaac tgcccggcga ttactgtgtg gacaccaagc tggacatcac ctcccacaac
      601 gaggactaca ccatcgtgga acagtacggg cgctccgagg gccgccaccg tctgggcatg
      661 gacgagcggt acaag
//
