LOCUS       sequence-409300-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dimerization dependent fluorescent protein GA anchored to cytosolic 
            face of the plasma membrane with the N-terminal targeting sequence 
            of human GAP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Miner GE, Smith SY, Showalter WK, So CM, Ragusa JV, Powers AE, 
            Zanellati MC, Hsu CH, Marchan MF, Cohen S
  TITLE     Contact-FP: A Dimerization-Dependent Fluorescent Protein Toolkit for
            Visualizing Membrane Contact Site Dynamics.
  JOURNAL   Contact (Thousand Oaks). 2024 Feb 4;7:25152564241228911. doi: 
            10.1177/25152564241228911. eCollection 2024 Jan-Dec.
  PUBMED    38327561
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Contact 
            (Thousand Oaks)."; date: "2024-02-4"; volume: "7:25152564241228911. 
            doi"; pages: " 10.1177/25152564241228911. eCollection 2024 Jan-Dec"
COMMENT     Sequence Label: PM-GA
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     596..619
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggtgagca agagcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcttggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccttacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc ccctgatcat gtacggctcc aagatgtacg tgaagcaccc cgccgacgtc
      241 cccgattaca tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgcacttc
      301 gaggacggcg gtctggtgac cgcgacacag gactcctccc tgcaggacgg cacgctgatc
      361 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      421 accttgggct gggattatgc caccgagcgc ctgtaccccg aagaaggcgt gctgaagggc
      481 gagctcctgg ggcgcctgaa gctgaaggac ggcggcctca acctggtgga gtccaagacc
      541 atctacatgg ccaagaagcc cgtgcaactg cccggctact acttcgtgga caccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
      661 cgccaccacc tggggcatgg caccggcagc acc
//
