LOCUS       sequence-409394-        9583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LEAPGW
FEATURES             Location/Qualifiers
     source          1..9583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2184..2395
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2396..3338
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3295..3315
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4429..4928
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4464..4487)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4861..4880
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(4946..4948,4949..4951,4952..5665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4991..5012)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5252..5271)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5599..5620
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5726..5746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6144..6155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6345..6978
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7107..7123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7107..7123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7120..7142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7131..7147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7155..7161,7162..7179,7180..7185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7200..7221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7338..7355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7509..8097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7589..7608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8268..9059,9060..9128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8891..8910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9129..9233)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9281..9415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9318..9337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9372..9391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9583,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2221 ggggggaggg gtcggcaatt gaaccggagc ctagagaagg tggcgcgggg taaactggga
     2281 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2341 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2401 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2461 aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     2521 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     2581 aggcctggct tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     2641 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     2701 ttttctggca agatagtctt gtaaatgcgg gccaagatcg gcacactggt atttcggttt
     2761 ttggggccgc gggcggcgac ggggccggtg cgtcccagcg cacatgttcg gcgaggcggg
     2821 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     2881 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     2941 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     3001 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     3061 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     3121 acctcgatta gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     3181 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     3241 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     3301 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc gcttcgacta
     3361 gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
     3421 atcttggaaa cacttgctgg gattacttcg acttcttaac ccaacagaag gctcgagaag
     3481 gtatattgct gttgacagga tccacattaa caactttaag tccagcaatt tgagttaagg
     3541 gtgttgctct caatgatttc actaatggtt caatttttaa tttcttttct tctggttcaa
     3601 aattcaagtt tcaagtgaaa gtgtaatatg cacccatttc tttcaataaa tcttctaaat
     3661 agtccactaa tgttttattt tgttttttac aaaatcaagc agcctctgct atcaatatag
     3721 aaccttgtat tccatcttta tctctagctg agtcatcaat tacatatcca taactttctt
     3781 cataagcaaa aacaaaattt aatccgttat cttcttcttt agcaatttct ctacccattc
     3841 atttcaatcc agttaaagtt tttacaatct taactccata tttttcctga gcgattctat
     3901 cacccaaatc acttgttaca aaacttgaat atagagccgg attttttgga atgctattta
     3961 agcgttttag attggataat tttcaatcaa ttaaaattgg tcctgtttga tttccatcta
     4021 atcttacaaa atgaccatca tgttttattg ccattccaaa tctgtcagca tctgggtcat
     4081 tcataataat aatatctgca tcatgtttaa taccatattc aagcggtatt tttcatgcag
     4141 gatcaaattc tggatttgga tttacaacat ttttcaatgt ttcatcttca aatgcacgat
     4201 cttcaccctc aataacgtta tatcctgatt cacgtaatct ttttggggta aatttagttc
     4261 ctgttccatt aactgcgcta aaaatagggc cctgcctcgg acttcaaggg gctagaattc
     4321 gagcaattat cttgtttact aaaactgaat accttgctat ctctttgata catttttaca
     4381 aagctgaatt aaaatggtat aaattaaatc acttttttca attctaccgg gtaggggagg
     4441 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     4501 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     4561 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     4621 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     4681 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     4741 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     4801 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     4861 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     4921 gcctttcgac ctttaattaa ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     4981 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtctgg
     5041 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5101 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     5161 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5221 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5281 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5341 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     5401 tatcatggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat
     5461 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     5521 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     5581 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     5641 cggcatggac gagctgtaca agtaagttta aacatcaacc tctggattac aaaatttgtg
     5701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6061 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6181 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6241 ccctttgggc cgcctccccg cctggcctgc aggggtacct ttaagaccaa tgacttacaa
     6301 ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca
     6361 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     6421 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     6481 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     6541 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     6601 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     6661 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     6721 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     6781 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6841 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6901 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6961 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7021 caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc
     7081 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7141 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7201 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7261 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7321 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7381 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7441 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7501 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7561 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7621 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7681 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7741 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7801 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7861 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7921 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7981 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8041 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8101 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8161 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8221 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8281 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8341 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8401 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8461 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8521 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8581 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8641 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8701 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8761 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8821 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8881 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8941 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9001 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9061 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9121 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9181 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9241 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
     9301 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9361 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     9421 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     9481 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     9541 aagaaattgt atttgttaaa tatgtactac aaacttagta gtt
//
