LOCUS       sequence-409400-       10191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with Azurite reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LAAPZW
FEATURES             Location/Qualifiers
     source          1..10191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        5040..5539
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5075..5098)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5472..5491
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(5860..5879)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(6334..6354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6752..6763)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6953..7586
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7715..7731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7715..7731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7728..7750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7739..7755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7763..7769,7770..7787,7788..7793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7808..7829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7946..7963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8117..8705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8197..8216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8876..9667,9668..9736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9499..9518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9737..9841)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9889..10023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9926..9945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9980..9999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10191,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgttccat gtccttatat ggactcatct ttgcctattg
     2221 cgacacacac tcaatgaaca cctactacgc gctgcaaaga gccccgcagg cctgaggtgc
     2281 ccccacctca ccactcttcc tatttttgtg taaaaatcca gcttcttgtc accacctcca
     2341 aggaggggga ggaggaggaa ggcaggttcc tctaggctga gccgaatgcc cctctgtggt
     2401 cccacgccac tgatcgctgc atgcccacca cctgggtaca cacagtctgt gattcccgga
     2461 gcagaacgga ccctgcccac ccggtcttgt gtgctactca gtggacagac ccaaggcaag
     2521 aaagggtgac aaggacaggg tcttcccagg ctggctttga gttcctagca ccgccccgcc
     2581 cccaatcctc tgtggcacat ggagtcttgg tccccagagt cccccagcgg cctccagatg
     2641 gtctgggagg gcagttcagc tgtggctgcg catagcagac atacaacgga cggtgggcct
     2701 agacccaggc tgtgtagacc cagccccccc gccccgcagt gactaggtca cccactaacg
     2761 ccccaggcct ggtcttggct gggcgtgact gttaccctca aaagcaggca gctccagggt
     2821 aaaaggtgcc ctgccctgta gagcccacct tccttcccag ggctgcggct gggtaggttt
     2881 gtagccttca tcacgggcca cctccagcca ctggaccgct ggcccctgcc ctgtcctggg
     2941 gagtgtggtc ctgcgacttc taagtggccg caagccacct gactccccca acaccacact
     3001 ctacctctca agcccaggtc tctccctagt gacccaccca gcacatttag ctagctgagc
     3061 cccacagcca gaggtcctca ggccctgctt tcagggcagt tgctctgaag tcggcaaggg
     3121 ggagtgactg cctggccact ccatgccctc caagagctcc ttctgcagga gcgtacagaa
     3181 cccagggcct tggcacccgt gcagaccctg gcccacccca cctgggcgct cagtgcccaa
     3241 gagatgtcca caactaggat gtcccgcggt gggtgggggg ccagagagac gggcaggccg
     3301 ggggcaggcc tggccatgcg gggccgaacc gggcactgcc cagcgtgggg cgcgggggcc
     3361 acggcgcgcg cccccagccc cccggcccag caccccaagg cggccaacgc caaaactctc
     3421 cctcctcctc ttcctcaatc tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga
     3481 gggggtaaaa aaatgctgca ctgtgcggcg aagccggtga gtgagcggcg cggggccaat
     3541 cagcgtgcgc cgttccgaaa gttgcctttt atggctcgaa cggccgcggc ggcgccctat
     3601 aaaacccagc ggcgcgacgc gccaccaccg ccgagaccgc gtccgccccg cgagcacaga
     3661 gcctcgcctt tgccgatccg ccgcccgtcc aggctcgcat ctctccttca cgcgcccgcc
     3721 gccttacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     3781 gtgcctcctg aactacgtcc gccgtctagg taagtttaga gctcaggtcg agaccgggcc
     3841 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     3901 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcag
     3961 cgcttcgact agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc
     4021 gttgcctgca catcttggaa acacttgctg ggattacttc gacttcttaa cccaacagaa
     4081 ggctcgagaa ggtatattgc tgttgacagg atccacatta acaactttaa gtccagcaat
     4141 ttgagttaag ggtgttgctc tcaatgattt cactaatggt tcaattttta atttcttttc
     4201 ttctggttca aaattcaagt ttcaagtgaa agtgtaatat gcacccattt ctttcaataa
     4261 atcttctaaa tagtccacta atgttttatt ttgtttttta caaaatcaag cagcctctgc
     4321 tatcaatata gaaccttgta ttccatcttt atctctagct gagtcatcaa ttacatatcc
     4381 ataactttct tcataagcaa aaacaaaatt taatccgtta tcttcttctt tagcaatttc
     4441 tctacccatt catttcaatc cagttaaagt ttttacaatc ttaactccat atttttcctg
     4501 agcgattcta tcacccaaat cacttgttac aaaacttgaa tatagagccg gattttttgg
     4561 aatgctattt aagcgtttta gatttgataa ttttcaatca attaaaattg gtcctgtttg
     4621 atttccatct aatcttacaa aatgaccatc atgttttatt gccattccaa atctgtcagc
     4681 atctgggtca ttcataataa taatatctgc atcatgttta ataccatatt caagcggtat
     4741 ttttcatgca ggatcaaatt ctggatttgg atttacaaca tttttcaatg tttcatcttc
     4801 aaatgcacga tcttcaccct caataacgtt atatcctgat tcacgtaatc tttttggggt
     4861 aaatttagtt cctgttccat taactgcgct aaaaataggg ccctgcctcg gacttcaagg
     4921 ggctagaatt cgagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat
     4981 acatttttac aaagctgaat taaaatggta taaattaaat cacttttttc aattctaccg
     5041 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5101 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5161 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5221 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5281 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5341 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5401 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5461 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5521 ctcatctccg ggcctttcga cctttaatta accaccatga gcaaaggcga agagctgttc
     5581 accggcgtgg tgcccatcct ggtggagctg gacggcgacg tgaacggcca caagttcagc
     5641 gtgagcggcg agggcgaggg cgacgccacc tacggcaagc tgaccctgaa gttcatctgc
     5701 accaccggca agctgcccgt gccctggccc accctggtga ccaccctgag ccacggcgtg
     5761 cagtgcttca gccggtaccc cgaccacatg aagcagcacg acttcttcaa gagcgccatg
     5821 cccgagggct acgtgcagga gcggaccatc ttcttcaagg acgacggcaa ctacaagacc
     5881 cgggccgagg tgaagttcga gggcgacacc ctggtgaacc ggatcgagct gaagggcatc
     5941 gacttcaagg aggacggcaa catcctgggc cacaagctgg agtacaactt caacagccac
     6001 aacatctaca tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgg
     6061 cacaacatcg aggacggcag cgtgcagctg gccgaccact accagcagaa cacccccatc
     6121 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagag cgccctgagc
     6181 aaggacccca acgagaagcg ggaccacatg gtgctgctgg agttccggac cgccgccggc
     6241 atcacccacg gcatggacga gctctataag taagtttaaa catcaacctc tggattacaa
     6301 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6361 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6421 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6481 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6541 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6601 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6661 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6721 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6781 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6841 tcggatctcc ctttgggccg cctccccgcc tggcctgcag gggtaccttt aagaccaatg
     6901 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     6961 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     7021 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     7081 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     7141 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     7201 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     7261 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     7321 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     7381 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     7441 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7501 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7561 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7621 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac
     7681 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7741 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7801 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7861 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7921 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7981 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8521 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9841 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt
     9901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    10021 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    10081 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
    10141 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt t
//
