LOCUS       sequence-409503-        9650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3-SREBP-2-GFP
FEATURES             Location/Qualifiers
     source          1..9650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(56..76)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        61..390
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      241..376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     303..322
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             457..1251
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(511..530)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1121..1140
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1425..1546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1462..1481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1516..1535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1595..1611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1595..1611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1608..1630)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1619..1635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1643..1649,1650..1667,1668..1673))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1688..1709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1826..1843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1997..2585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2077..2096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2756..3547,3548..3616))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3379..3398
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3617..3721)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3796..3815)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3987..4366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4367..4570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4521..4541
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4615..4634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4615..4633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4669..4678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4675..4707
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             4708..4740
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             4741..4773
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(8233..8235,8236..8946)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8233..8235,8236..8946)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8275..8296)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8536..8555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8883..8904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8993..9010)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8999..9223
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9269..9650,1..47)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9356..9375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9566..9587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
       61 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
      121 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      181 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      241 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      301 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
      361 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
      421 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
      481 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
      541 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
      601 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
      661 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
      721 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
      781 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
      841 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
      901 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
      961 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     1021 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     1081 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     1141 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     1201 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     1261 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     1321 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     1381 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     1441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     1561 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1621 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1681 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1741 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1801 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2401 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3061 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3241 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3301 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3361 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3421 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3481 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3541 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3601 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3661 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3721 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     3781 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     3841 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     3901 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     3961 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     4021 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     4081 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     4141 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     4201 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     4261 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     4321 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     4381 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     4441 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     4501 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     4561 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
     4621 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc caccatggct
     4681 agcatgactg gtggacagca aatgggtatg gctagcatga ctggtggaca gcaaatgggt
     4741 atggctagca tgactggtgg acagcaaatg ggtgcatcga taggtaccgg tgacgacagc
     4801 ggcgagctgg gtggtctgga gaccatggag accctcacgg agctgggcga cgagctgacc
     4861 ctgggagaca tcgacgagat gctgcaattt gtcagtaatc aagtgggaga gttccctgac
     4921 ttgttttcag aacagctgtg tagctccttt cctggcagtg gtggtagtgg tagcagcagc
     4981 ggcagcagtg gcagcagcag cagcagcagc aatggcaggg gcagcagcag cggagctgtg
     5041 gacccttcag tgcaacggtc attcacccag gtcacattac cttccttctc tccctcggcg
     5101 gcctccccac aggctccaac tctgcaagtc aaggtttctc ccacctcagt tcccaccaca
     5161 cccagggcaa ctcctattct tcagccccgc ccccagcccc agcctcaacc tcaaactcag
     5221 ctgcaacaac agacggtaat gatcacgcca acattcagca ccactccgca gacgaggatc
     5281 atccagcagc ctttgatata ccagaatgca gctactagct ttcaagtcct tcagcctcaa
     5341 gtccaaagcc tggtgacatc ctcccaggta cagccggtca ccattcagca gcaggtgcag
     5401 acagtacagg cccagcgggt gctgacacaa acggccaatg gcacgctgca gacccttgcc
     5461 ccggctacgg tgcagacagt tgctgcgcca caggtgcagc aggtcccggt cctggtccag
     5521 cctcagatca tcaagacaga ttcccttgtt ttgaccacac tgaagacaga tggcagccct
     5581 gttatggctg cggtccagaa cccggccctc accgccctca ccacccctat ccagacggct
     5641 gcccttcaag taccaaccct ggtgggcagc agtgggacca ttctgaccac aatgcctgta
     5701 atgatggggc aagagaaagt gcccattaag caggtacctg ggggagtcaa gcagcttgag
     5761 ccccccaaag aaggagaaag gcggacaacc cataatatca ttgagaaacg atatcgctcc
     5821 tccatcaatg acaaaatcat cgaattgaaa gacctggtca tggggacaga cgccaagatg
     5881 cacaagtctg gcgttctgag gaaggccatt gattacatca aatacttgca gcaggtcaat
     5941 cataaactgc gccaggagaa catggtgctg aagctggcaa atcaaaagaa caagcttcta
     6001 aagggcatcg acctaggcag tctggtggac aatgaggtgg acctgaagat cgaggacttt
     6061 aatcagaatg tccttctgat gtccccccca gcctctgact cagggtccca ggctggcttc
     6121 tctccctact ccattgactc tgagccagga agccctctat tggatgatgc aaaggtcaaa
     6181 gatgagccag actctcctcc tgtggcgctg ggcatggtag accgctcacg gattcttctg
     6241 tgtgtcctca ccttcctgtg cctctccttt aaccccctga cttccctgct gcagtgggga
     6301 ggggcccacg actctgacca gcacccacac tcaggctctg gccgcagtgt cctgtcattc
     6361 gagtcaggtt ctgggggctg gtttgactgg atgatgccta ctcttctctt atggctggta
     6421 aatggtgtga ttgtcctgag cgtctttgtg aagctgctgg ttcatgggga gccagtgatc
     6481 cggccacact cgcgctcctc ggtcaccttc tggaggcacc ggaaacaggc agatctggat
     6541 ctcgccagag gagattttgc agctgctgcc ggcaacctac aaacctgcct ggcagttttg
     6601 ggccgggcac tgcccacctc ccgcctggac ctggcctgca gcctctcctg gaacgtgatc
     6661 cgctacagcc tgcagaagct acgcctggtg cgctggctgc tcaagaaagt cttccagtgc
     6721 cggcgggcca cgccagccac tgaggcaggc tttgaagacg aagctaagac cagcgcccgg
     6781 gatgcggctc tggcctatca ccggctgcac cagctgcaca tcacagggaa gcttcctgca
     6841 ggatccgcct gttccgatgt acacatggcg ttgtgtgccg tgaacctggc tgaatgtgca
     6901 gaggagaaga tcccaccgag cacactggtt gagatccatc tgactgctgc catggggctc
     6961 aagacccggt gtggaggcaa gctgggcttc ctggccagct acttcctcag ccgagcccag
     7021 agcctgtgtg gccccgagca cagtgctgtt cctgactccc tgcgctggct ctgccacccc
     7081 ctgggccaga agtttttcat ggagcggagc tggtctgtga agtcagctgc caaggagagt
     7141 ctatactgtg cccagaggaa cccagctgac cccattgcgc aggtccacca ggccttctgc
     7201 aagaacctgc tggagcgagc tatagagtcc ttggtgaaac ctcaggccaa gaagaaggct
     7261 ggagaccagg aagaagagag ctgtgaattc tccagtgctc tggagtactt gaaattactt
     7321 cattcttttg tggactctgt gggggttatg agccccccac tctccaggag ctccgtgctc
     7381 aagtccgccc tgggtccaga catcatctgt cggtggtgga cgtctgcaat cactgtggcc
     7441 atcagctggc tccagggaga cgatgcagct gtgcgctctc attttaccaa agtggaacgc
     7501 atccccaagg ccctggaagt gacagagagc cccctggtga aggccatctt ccatgcctgc
     7561 agagccatgc atgcctcact ccctgggaaa gcagatgggc agcagagttc cttctgccat
     7621 tgcgagaggg ccagtggcca cctatggagc agcctcaacg tcagtggggc cacctctgac
     7681 cctgccctca accacgtggt ccagctgctc acctgtgacc tgctactgtc gctacggaca
     7741 gcgctctggc aaaaacaggc cagtgccagc caggctgtgg gggagaccta ccacgcgtca
     7801 ggcgctgaac tggcgggctt ccaacgggac ctgggcagcc tgcgcaggct ggcacacagc
     7861 ttccgcccag cataccgcaa ggtgttcctg catgaagcca ccgtgcgcct gatggcagga
     7921 gccagcccca cccgcaccca ccagctgctg gaacacagcc tgcggcggcg caccacgcag
     7981 agcaccaagc acggagaggt ggatgcctgg cccggccagc gagagcgggc caccgccatc
     8041 ctgctggcct gccgccacct gcccctctcc ttcctctcct ccccgggcca gcgggcagtg
     8101 ctgctggccg aagctgcccg caccctggag aaggtgggcg accggcgctc ctgcaacgac
     8161 tgccagcaga tgattgttaa gctgggtggt ggcactgcca ttgccgcctc cggaagtgcg
     8221 gccgcaggca gcgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     8281 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     8341 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     8401 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     8461 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     8521 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     8581 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     8641 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     8701 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     8761 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     8821 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     8881 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     8941 tacaagtccg gactctagta atctagaggg cccgtttaaa cccgctgatc agcctcgact
     9001 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     9061 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     9121 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     9181 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     9241 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     9301 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9361 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9421 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9481 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9541 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9601 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
//
