LOCUS       sequence-409507-        8948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3-SREBP-2
FEATURES             Location/Qualifiers
     source          1..8948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      175..310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     237..256
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             391..1185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(445..464)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1055..1074
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1359..1480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1396..1415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1450..1469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1529..1545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1529..1545)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1542..1564)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1553..1569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1577..1583,1584..1601,1602..1607))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1622..1643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1760..1777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1931..2519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2011..2030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2690..3481,3482..3550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3313..3332
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3551..3655)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3730..3749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3921..4300
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4301..4504
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4455..4475
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4549..4568
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4549..4567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4603..4612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4609..4641
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             4642..4674
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             4675..4707
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(8225..8242)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8231..8455
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8501..8929
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8588..8607)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8798..8819
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(8938..8948,1..10))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(8943..8948,1..324)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
       61 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      241 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
      301 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
      361 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
      421 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
      481 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
      541 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
      601 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
      661 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
      721 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
      781 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
      841 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
      901 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
      961 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     1021 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     1081 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     1141 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     1201 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     1261 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     1321 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     1381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     1501 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1561 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1621 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1681 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1741 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1801 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1861 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1921 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1981 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2041 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2101 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2161 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2221 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2281 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2341 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2401 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2461 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2521 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2581 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2641 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2701 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2761 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2821 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2881 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2941 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3001 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3061 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3121 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3181 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3241 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3301 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3361 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3421 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3481 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3541 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3601 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3661 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     3721 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     3781 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     3841 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     3901 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     3961 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4021 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4081 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4141 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4201 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4261 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     4321 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4381 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     4441 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     4501 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     4561 ctatagggag acccaagctg gctagcgttt aaacttaagc ttgccaccat ggctagcatg
     4621 actggtggac agcaaatggg tatggctagc atgactggtg gacagcaaat gggtatggct
     4681 agcatgactg gtggacagca aatgggtgca tcgataggta ccggtgacga cagcggcgag
     4741 ctgggtggtc tggagaccat ggagaccctc acggagctgg gcgacgagct gaccctggga
     4801 gacatcgacg agatgctgca atttgtcagt aatcaagtgg gagagttccc tgacttgttt
     4861 tcagaacagc tgtgtagctc ctttcctggc agtggtggta gtggtagcag cagcggcagc
     4921 agtggcagca gcagcagcag cagcaatggc aggggcagca gcagcggagc tgtggaccct
     4981 tcagtgcaac ggtcattcac ccaggtcaca ttaccttcct tctctccctc ggcggcctcc
     5041 ccacaggctc caactctgca agtcaaggtt tctcccacct cagttcccac cacacccagg
     5101 gcaactccta ttcttcagcc ccgcccccag ccccagcctc aacctcaaac tcagctgcaa
     5161 caacagacgg taatgatcac gccaacattc agcaccactc cgcagacgag gatcatccag
     5221 cagcctttga tataccagaa tgcagctact agctttcaag tccttcagcc tcaagtccaa
     5281 agcctggtga catcctccca ggtacagccg gtcaccattc agcagcaggt gcagacagta
     5341 caggcccagc gggtgctgac acaaacggcc aatggcacgc tgcagaccct tgccccggct
     5401 acggtgcaga cagttgctgc gccacaggtg cagcaggtcc cggtcctggt ccagcctcag
     5461 atcatcaaga cagattccct tgttttgacc acactgaaga cagatggcag ccctgttatg
     5521 gctgcggtcc agaacccggc cctcaccgcc ctcaccaccc ctatccagac ggctgccctt
     5581 caagtaccaa ccctggtggg cagcagtggg accattctga ccacaatgcc tgtaatgatg
     5641 gggcaagaga aagtgcccat taagcaggta cctgggggag tcaagcagct tgagcccccc
     5701 aaagaaggag aaaggcggac aacccataat atcattgaga aacgatatcg ctcctccatc
     5761 aatgacaaaa tcatcgaatt gaaagacctg gtcatgggga cagacgccaa gatgcacaag
     5821 tctggcgttc tgaggaaggc cattgattac atcaaatact tgcagcaggt caatcataaa
     5881 ctgcgccagg agaacatggt gctgaagctg gcaaatcaaa agaacaagct tctaaagggc
     5941 atcgacctag gcagtctggt ggacaatgag gtggacctga agatcgagga ctttaatcag
     6001 aatgtccttc tgatgtcccc cccagcctct gactcagggt cccaggctgg cttctctccc
     6061 tactccattg actctgagcc aggaagccct ctattggatg atgcaaaggt caaagatgag
     6121 ccagactctc ctcctgtggc gctgggcatg gtagaccgct cacggattct tctgtgtgtc
     6181 ctcaccttcc tgtgcctctc ctttaacccc ctgacttccc tgctgcagtg gggaggggcc
     6241 cacgactctg accagcaccc acactcaggc tctggccgca gtgtcctgtc attcgagtca
     6301 ggttctgggg gctggtttga ctggatgatg cctactcttc tcttatggct ggtaaatggt
     6361 gtgattgtcc tgagcgtctt tgtgaagctg ctggttcatg gggagccagt gatccggcca
     6421 cactcgcgct cctcggtcac cttctggagg caccggaaac aggcagatct ggatctcgcc
     6481 agaggagatt ttgcagctgc tgccggcaac ctacaaacct gcctggcagt tttgggccgg
     6541 gcactgccca cctcccgcct ggacctggcc tgcagcctct cctggaacgt gatccgctac
     6601 agcctgcaga agctacgcct ggtgcgctgg ctgctcaaga aagtcttcca gtgccggcgg
     6661 gccacgccag ccactgaggc aggctttgaa gacgaagcta agaccagcgc ccgggatgcg
     6721 gctctggcct atcaccggct gcaccagctg cacatcacag ggaagcttcc tgcaggatcc
     6781 gcctgttccg atgtacacat ggcgttgtgt gccgtgaacc tggctgaatg tgcagaggag
     6841 aagatcccac cgagcacact ggttgagatc catctgactg ctgccatggg gctcaagacc
     6901 cggtgtggag gcaagctggg cttcctggcc agctacttcc tcagccgagc ccagagcctg
     6961 tgtggccccg agcacagtgc tgttcctgac tccctgcgct ggctctgcca ccccctgggc
     7021 cagaagtttt tcatggagcg gagctggtct gtgaagtcag ctgccaagga gagtctatac
     7081 tgtgcccaga ggaacccagc tgaccccatt gcgcaggtcc accaggcctt ctgcaagaac
     7141 ctgctggagc gagctataga gtccttggtg aaacctcagg ccaagaagaa ggctggagac
     7201 caggaagaag agagctgtga attctccagt gctctggagt acttgaaatt acttcattct
     7261 tttgtggact ctgtgggggt tatgagcccc ccactctcca ggagctccgt gctcaagtcc
     7321 gccctgggtc cagacatcat ctgtcggtgg tggacgtctg caatcactgt ggccatcagc
     7381 tggctccagg gagacgatgc agctgtgcgc tctcatttta ccaaagtgga acgcatcccc
     7441 aaggccctgg aagtgacaga gagccccctg gtgaaggcca tcttccatgc ctgcagagcc
     7501 atgcatgcct cactccctgg gaaagcagat gggcagcaga gttccttctg ccattgcgag
     7561 agggccagtg gccacctatg gagcagcctc aacgtcagtg gggccacctc tgaccctgcc
     7621 ctcaaccacg tggtccagct gctcacctgt gacctgctac tgtcgctacg gacagcgctc
     7681 tggcaaaaac aggccagtgc cagccaggct gtgggggaga cctaccacgc gtcaggcgct
     7741 gaactggcgg gcttccaacg ggacctgggc agcctgcgca ggctggcaca cagcttccgc
     7801 ccagcatacc gcaaggtgtt cctgcatgaa gccaccgtgc gcctgatggc aggagccagc
     7861 cccacccgca cccaccagct gctggaacac agcctgcggc ggcgcaccac gcagagcacc
     7921 aagcacggag aggtggatgc ctggcccggc cagcgagagc gggccaccgc catcctgctg
     7981 gcctgccgcc acctgcccct ctccttcctc tcctccccgg gccagcgggc agtgctgctg
     8041 gccgaagctg cccgcaccct ggagaaggtg ggcgaccggc gctcctgcaa cgactgccag
     8101 cagatgattg ttaagctggg tggtggcact gccattgccg cctcctgagg atccgaattc
     8161 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga
     8221 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     8281 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     8341 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     8401 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     8461 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     8521 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8581 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8641 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8701 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8761 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8821 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8881 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     8941 atgtgtgt
//
