LOCUS       sequence-409510-        2398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AvrSr27-1 as Golden Gate compatible MoClo CDS part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2398)
  TITLE     Schwessinger Lab Plasmids 2023
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBD025
FEATURES             Location/Qualifiers
     source          1..2398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(442..458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(442..458)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(455..477)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(630..1218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(710..729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1351..1445)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1466..2125)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2040..2059
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2126..2228)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2315..2337
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2329..2346
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2330..2346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctctgtggtc tcaaatgatg acaccacatc accaaagtaa ttgcaactcc ccatctttga
       61 aatttcccag gttcattgga aaatgtgact cctgccagct ccataccaaa gctaccaacc
      121 tggtgagctg cacctcttgt aggaaatcct cattggtata tgaagaatgt tccaccaaag
      181 gctgtcctgc taattggcac aaaagcacct gtcaagaacc caagttcaat agaggtattc
      241 tgtcctgtta ctgtgagaac tgccagcagc acaccaagga aaaacagaca atttcctgca
      301 aaaattgtaa gaattcagcc accaccttct cacattgttc aagcccagag tgtcacagca
      361 gatggtaagc ttcgagacca cgaagaggct aggtggaggc tcagtgatga taagtctgcg
      421 atggtggatg catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcag
      481 agggcacaat cctattccgc gctatccgac aatctccaag acattaggtg gagttcagtt
      541 cggcgtatgg catatgtcgc tggaaagaac atgtgagcaa aaggccagca aaaggccagg
      601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctctattc
     1261 aacaaagccg ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa atcgtccccc
     1321 catacgatat aagttgttac tagtgcttgg attctcacca ataaaaaacg cccggcggca
     1381 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     1441 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     1501 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1561 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1621 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1681 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1741 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1801 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1861 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     1921 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1981 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     2041 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     2101 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     2161 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     2221 gccgatcaac gtctcattga tacctgaaac aaaacccatc gtacggccaa ggaagtctcc
     2281 aataactgtg atccaccaca agcgccaggg ttttcccagt cacgacgttg taaaacgacg
     2341 gccagtcatg cataatccgc acgcatctgg aataaggaag tgccattccg cctgacct
//
