LOCUS       sequence-409515-        2625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  D6: Promoter of TraesCS3B02G578800 as Golden Gate compatible MoClo 
            PROM part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2625)
  TITLE     Schwessinger Lab Plasmids 2023
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PROM_6
FEATURES             Location/Qualifiers
     source          1..2625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(669..685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(682..704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(857..1445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(937..956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1578..1672)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1693..2352)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2267..2286
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2353..2455)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2542..2564
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2556..2573
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2557..2573
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctctgtggtc tcaggagaaa tccttagccg tgtagcaccg actgtcggac cgacgcggtt
       61 aagctaggta gcatgcaaac atactcggtc gtgttcgagt agtctccatt gctactggag
      121 atcaagccgc gtgcgtgtca ttatccatct caagaaaacg cgcgacggcg attgatggag
      181 ccacactacg aaagcgaaat atatttgaga tcggtccgta tcgatgcggt atgaacaaat
      241 tgaaaaaaca aatctgtgac gtgagtactc aagcaaacac aaaaaacatc ttatatttgt
      301 gatcagaagg gtagctaaaa gcacacgtcg aaaaagttga gaacgcgtaa gaggaacgcg
      361 cgtaggtacg tgcgagtcag ctgcatatgc tagctccgcc cgtacatgat cttctccatc
      421 ctatataaag agacatgcat gccctcatgt caagcacata tcacaaacac acatacaaac
      481 acagatcctc atcctccatt cctccggtat aaccgtcgat cacacaaagc aaggcacgag
      541 tgcgtacgca cgcacacgca cgtactccgt tgagagctcg gaaggctaat tggtgaatgc
      601 gagaccacga agaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat
      661 gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct
      721 attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat
      781 atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1441 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg
     1501 tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtccccccat acgatataag
     1561 ttgttactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     1621 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     1681 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1741 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1801 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1861 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     1921 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1981 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     2041 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2101 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     2161 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2221 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2281 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2341 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     2401 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     2461 tcattgatac ctgaaacaaa acccatcgta cggccaagga agtctccaat aactgtgatc
     2521 caccacaagc gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtcatgcat
     2581 aatccgcacg catctggaat aaggaagtgc cattccgcct gacct
//
