LOCUS       sequence-409539-        4599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DV2 prM-E structural cassette in pVRC8400.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Chao LH, Jang J, Johnson A, Nguyen A, Gray NS, Yang PL, Harrison SC
  TITLE     How small-molecule inhibitors of dengue-virus infection interfere 
            with viral membrane fusion.
  JOURNAL   Elife. 2018 Jul 12;7. pii: 36461. doi: 10.7554/eLife.36461.
  PUBMED    29999491
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-12"; volume: "7. pii: 36461. doi"; pages: " 
            10.7554/eLife.36461"
COMMENT     Sequence Label: DV2 VLPs pVRC8400
FEATURES             Location/Qualifiers
     source          1..4599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(142..164)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     264..283
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     477..499
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2622..2638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2622..2638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2635..2657)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2646..2662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2670..2676,2677..2694,2695..2700))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2715..2736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2853..2870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3024..3612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3104..3123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3790..4599)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4513..4532
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     join(4591..4599,1..11)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
       61 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gcatcgcgcg
      121 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
      181 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
      241 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
      301 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
      361 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      421 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      481 gtcacgacgt tgtaaaacga cggccagaga attcgagctc ggtacctcgc gaatacatct
      541 agatccaatc caatgctagc ttccatctga ccacccgaaa cggcgaaccc catatgattg
      601 tgtccaggca ggagaagggc aagagtctgc tgttcaaaac tgaggatggc gtgaacatgt
      661 gcaccctgat ggctatggac ctgggggagc tgtgcgaaga taccatcaca tacaaatgtc
      721 ctttcctgaa gcagaacgag ccagaagaca ttgattgctg gtgtaatagt acttcaacct
      781 gggtgacata tgggacttgt accacaactg gagagcaccg gagagaaaag cgatctgtcg
      841 cactggtgcc acatgtcgga atgggactgg agacacgcac tgaaacctgg atgagctccg
      901 agggagcatg gaagcacgct cagcgcattg aaacctggat cctgcgacat ccaggcttca
      961 ccattatggc cgctatcctg gcatacacta tcggcaccac acacttccag agagccctga
     1021 tttttatcct gctgactgct gtggcaccct ctatgaccat gcggtgcatt gggatcagta
     1081 acagagactt cgtggaggga gtctccggcg ggtcttgggt ggatattgtc ctggaacatg
     1141 ggagctgtgt gactaccatg gcaaagaaca agcctaccct ggactttgag ctgatcaaaa
     1201 cagaagcaaa gcagccagcc actctgcgga aatattgcat tgaggccaag ctgactaaca
     1261 caactaccga ttcaagatgt cccacccagg gcgaacctag cctgaatgag gaacaggaca
     1321 aaaggttcgt gtgcaagcac tccatggtcg atcgcggatg gggcaacggg tgtggactgt
     1381 tcggcaaggg aggcatcgtg acttgcgcca tgtttacctg taagaaaaac atgaagggga
     1441 aagtggtcca gccagagaat ctggaataca ctattgtgat caccccccac tctggcgagg
     1501 aacatgccgt cgggaatgac acagggaagc acggaaaaga gattaagatc acacctcagt
     1561 ctagtatcac tgaggctgaa ctgacaggct atgggactgt gaccatggag tgcagcccca
     1621 ggaccggcct ggatttcaac gagatggtgc tgctgcagat ggaaaataag gcttggctgg
     1681 tccatcgcca gtggtttctg gacctgccac tgccatggct gccaggagca gatacccagg
     1741 gcagtaactg gatccagaaa gagaccctgg tgacattcaa gaatcctcac gccaagaaac
     1801 aggacgtggt cgtgctggga tcccaggagg gagctatgca tactgcactg accggcgcca
     1861 cagaaattca gatgtcaagc ggaaatctgc tgttcacagg ccacctgaaa tgcaggctgc
     1921 gcatggataa actgcagctg aagggaatga gctactccat gtgtaccggc aagtttaaag
     1981 tcgtgaagga gatcgccgaa acccagcacg gcacaattgt gatcagggtc cagtatgagg
     2041 gagacggcag cccttgtaaa attccatttg agatcatgga tctggaaaag agacatgtgc
     2101 tgggaaggct gattaccgtg aaccccatcg tcacagagaa ggactcccct gtgaatatcg
     2161 aggctgaacc ccctttcggc gattcttaca tcattatcgg agtggagcca ggccagctga
     2221 aactgaactg gtttaagaaa gggtcctcta ttggacagat gatcgaaaca actatgcgag
     2281 gcgctaagcg gatggcaatc ctgggggaca cagcatggga tttcggaagc ctggggggag
     2341 tgtttacctc cattgggaaa gccctgcacc aggtgttcgg cgctatctat ggggcagcct
     2401 ttagtggagt gtcatggatt atgaagattc tgatcggcgt cattatcacc tggatcggga
     2461 tgaattctcg gagtacatca ctgagcgtgt ctctggtgct ggtcggggtc gtcactctgt
     2521 atctgggggt catggtgcag gcataagcgg ccgcattgga ttggatcgga tcccgggccc
     2581 gtcgactgca gaggcctgca tgcaagcttg gtgtaatcat ggtcatagct gtttcctgtg
     2641 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2701 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2761 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2821 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2881 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2941 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3721 ttaaattaaa aatgaagttt taaatcaagc ccaatctgaa taatgttaca accaattaac
     3781 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     3841 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     3901 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     3961 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     4021 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     4081 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     4141 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     4201 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     4261 atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa
     4321 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     4381 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     4441 tttcagaaac aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga
     4501 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     4561 taatcgcggc ctcgacgttt cccgttgaat atggctcat
//
