LOCUS       sequence-409555-        8822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cup expression for Schneider cell imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: I14-pAc5.1 lambda N HA Cup
FEATURES             Location/Qualifiers
     source          1..8822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(358..1149,1150..1218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     981..1000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1219..1323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1391..1409
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1473..3982
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             4002..4067
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             4092..4118
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7545..7586
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7596..7613
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7636..7653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7686..7820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7710..7729)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7764..7783
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(join(8421..8822,1..187))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8501..8520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
       61 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      241 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      301 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      361 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      421 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      481 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      541 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      601 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      661 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      721 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      781 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      841 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      901 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      961 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1021 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1081 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1141 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1201 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1261 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1321 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     1381 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttctatattc
     1441 taaaaacaca aatgatactt ctaaaaaaaa atcatgaatg gcatcaactc tgaatcaaat
     1501 ctttgcagat gcacctactt ctcatttcca ctgtcacatc atttttccag atctcgctgc
     1561 ctgttatgtg gcccacaaac caagacacgt tttatggcca ttaaagctgg ctgatcgtcg
     1621 ccaaacacca aatacataat gaatatgtac acattcgaga aagaagcgat caaagaagcg
     1681 tcttcgggcg gagtaggaga atgcggagga gaaggagaac gagctgatct agtatctctc
     1741 cacaatccaa tgccaactga ccaactggcc atattcggag caatttgaag ccaatttcca
     1801 tcgcctggcg atcgctccat tcttggctat atgtttttca ccgttacccg gggccatttt
     1861 caaagactcg tcggcaagat aagattgtgt cactcgctgt ctctcttcat ttgtcgaaga
     1921 atgctgagga atttcgcgat gacgtcggcg agtattttga agaatgagaa taatttgtat
     1981 ttatacgaaa atcagttagt ggaattttct acaaaaacat gttatctata gataattttg
     2041 ttgcaaaata tgttgactat gacaaagatt gtatgtatat acctttaatg tattctcatt
     2101 ttcttatgta tttataatgg caatgatgat actgatgata ttttaagatg atgccagacc
     2161 aaaaggcttg aatttctgcg tcttttgccg aacgcagtgc atgtgcaatt gttgtttttt
     2221 ggaatattca attttcggac tgtccgcttt gatttcagtt tcttggctta ttcaaaaagc
     2281 aaagtaaagc caaaaaagcg agatggcaat accaaatgcg gcaaaacggt agtggaagga
     2341 aaggggtgcg gggcagcgga aggaagggtg gggcggggcg tggcggggtc tgtggctggg
     2401 cgcgacgtca ccgacgttgg agccactcct ttgaccatgt gtgcgtgtgt gtattattcg
     2461 tgtctcgcca ctcgccggtt gtttttttct ttttatgctg cgctctctct agcgccatct
     2521 cgcttacgca tgctcaacgc accgcatgtt gccgtttcct tttatgcgtc attttggctc
     2581 gaaataggca attatttaaa caaagattag tcaacgaaaa cgctaaaata aataagtcta
     2641 caatatggtt acttattgcc atgtgtgtgc agccaacgat agcaacaaaa gcaacaacac
     2701 aggtggcttt ccctctttca ctttttgttt gcaagccgcg tgcgagcaag acggcacgac
     2761 cggcaaacgc aattacgctg acaaagagca gacgaagttt tggcgaaaaa catcaaggcg
     2821 cctgatacga atgcatttgc aataacaatt gcgatattta atattgttta tgaagctgtt
     2881 tgacttcaaa acacacaaaa aaaaaaataa aacaaattat ttgaaagaga attaggaatc
     2941 ggacgcttat cgttagggta acaacaagaa atgcttactg agtcacagcc tctggaaaac
     3001 tgccgcaagc cagagagaga gagaaaaaga gggagagcag cttagaccgc atgtgcttgt
     3061 gtgtgaggcg tctctctctt cgtctctgtt gcgcaaacgc atagactgca ctgaaaaaat
     3121 cgattaccta ttttttatga atgaatattt gcactattac tattcaaaac tattaagata
     3181 gcaatcacat tcaatagcca aatactatac cacctgagcg atgcaacgaa atgatcaatt
     3241 tgagcaaaaa tgctgcatat ttaggacggc atcattatag aaatgcttct tgctgtgtac
     3301 ttttctctcg tctggcagct gtttcgccgt tattgttaaa accggcttaa gttaggtgtg
     3361 ttttctacga ctagtgaatg ccctactaga agatgtgtgt tgcacaaaat gtccctggaa
     3421 taaccaattt gaagtgcaga tagcagtaaa cgtaagctaa tatgaatatt atttaactgt
     3481 aatgttttaa tatcgctgga cattactaat aaacccacta taaacacatg tacatatgta
     3541 tgttttggca tacaatgagt agttggggaa aaaatgtgta aaagcaccgt gaccatcaca
     3601 gcataaagat aaccagctga agtatcgaat atgagtaacc cccaaattga atcacatgcc
     3661 gcaactgata ggacccatgg aagtacactc ttcatggcga tatacaagac acacacaagc
     3721 acgaacaccc agttgcggag gaaattctcc gtaaatgaaa acccaatcgg cgaacaattc
     3781 atacccatat atggtaaaag ttttgaacgc gacttgagag cggagagcat tgcggctgat
     3841 aaggttttag cgctaagcgg gctttataaa acgggctgcg ggaccagttt tcatatcact
     3901 accgtttgag ttcttgtgct gtgtggatac tcctcccgac acaaagccgc tccatcagcc
     3961 agcagtcgtc taatccagag accccggatc ggggtaccaa catggacgca caaacacgac
     4021 gacgtgagcg tcgcgctgag aaacaagctc aatggaaagc tgcaaacggt ggatctcctc
     4081 cacatatggc ttacccatac gatgttccag attacgctcc tccaagcttg aattctgcag
     4141 atatgcaaat ggccgaagct gagcaagaaa atggcgctgg cgccctgaaa attgccacca
     4201 atgcgggtgc aaccgatcgt ccagcgcatc agcagcttcc gctgccagtc gaagatcagc
     4261 aggatgaggt gctgaccccc gctgagaagg gcaagttcga gtaccctccc cctccaccgc
     4321 caccaactcc agtccaggct ccgctagcca ccaaggccac cgctctgaat gccagtcaag
     4381 agcatgacga cgacgaggcc aactccgaga agtgggagga tccgtgcgct ccgcccccgc
     4441 ctcctccgct gccgactagc gctttcctgg ccaccggact gggctacctc aagctgcccg
     4501 ccttcaagct gaaggacgca ctggagaagg ccatcaccaa gttggaggcc aacaagcgga
     4561 cgctaaaggc cagccctgag tcttctcgtt ccatcaaaaa caagaatgtg gtggccctgg
     4621 agatgttacc caggcgctca aacccagaaa cgatcggcga tggatccatg ttggcatcca
     4681 catccacggc tgttatgctg cagaccaaaa agcccgccgt tattgtggag atggagaggc
     4741 gttgtaagat catcaattta ctggccaaac agaatcaaat cctcgaatcc atttcggggg
     4801 aggccattcc gatgcatgga ccctcgaagc atctgcacga ggacgagggt ctgaccctgc
     4861 aagttctctc ggcccgagca tccacaccgt acacacagcc ctccagcatg ttgtcctgca
     4921 cggccgttag ttgtgacctg gagcacgatt cgccccggaa gcaagtggcc agcaaggagg
     4981 ccgtgccgga gcagcagtct agtcaggtgc agcaaaagcg tccacccagc acgggcattc
     5041 acaagccagg aagtctgcga gcacccaagg ctgttcgtcc tactactgct ccggtggtca
     5101 gcagcaagcc ggtcaagagc tatacccgct cccgcctgat ggacattcgc aacgggatgt
     5161 tcaatgccct gatgcacagg tccaaggaga gctttgtgat gcctcgcatc gccacctgcg
     5221 acgacattga gttggaggga aggcttcgcc gcatgaatat ttggcgcacc tcggacggca
     5281 caagattccg gacccgctcc accacagcta acttaaacat gaacaacaac aacaacaatg
     5341 agtgcatgcc tgccttcttc aaaaacaaaa ataagccgaa cctaatcagt gacgagtcca
     5401 tcatccagag ccaaccgccc cagccgcaaa cggagttcca agatccggcc attgttaacc
     5461 aaagacgcat tggcagcggt cgcttaaacc attccaagtg gggttacaac gacgaagact
     5521 accactcgta ccacaatggc aagtcgcagc atatggagga ggtcaactcg aagaacagca
     5581 aaaacatgac ggtgctgcag ttcttcgaca acggcgagat cagcagtcag cctcaaagac
     5641 ggccgaacac tcctgtgatg gggatgtcta tcaatcgatc cgagaacgac accctgcact
     5701 cgaacgagtc cagtgaagat ttgagtcgcg ccaacgagaa ctatgtaaag cgcgtaatgt
     5761 ctggattcct ggttgtatcg aagcccaagt cacgagatgt cgaggataga catcaccggc
     5821 ggtacaggaa ccagaacgag gagcctgagt ggtttagctg tggacccacg tctagactgg
     5881 acactataga gttgtgtggg ttcgatgagg acgaggagaa aatgctaaag gagggcaaca
     5941 aaaaccacgg attaggcgaa acagagcgag aaacgtcaaa acaaaagatg gaccataagt
     6001 acaaatggac gcatgcggag ccgatgggtc gaaacaaata catgcccaaa cacgacacta
     6061 acaataatca taacgtggag aacatgaaca atgttatggc taccgagcat cagcaacaaa
     6121 aggaggagaa acgtccaggc agtggccgtt cgtttcagtt tgataagttc aatcaaagcc
     6181 aacagaatta cgagagtagc agttatgtta atcaccaaca gccgccgcaa acgcagcctc
     6241 agcaaatgca gcagcaatcg aacacaaaca cgaacaactc taaattcatg tcattctttg
     6301 ccaatgaggg gaactcctcg tcttcttcgc tgaacgagtt tttcaagcag gccattaacc
     6361 agggtcatgg caacaatccg gagcagccca aatcgttggg ccacatcgga cagatgcctt
     6421 cggtggatca gctggaggcc aaatggcgtc gaaactcact gaacaatgtt ggcgagactg
     6481 ccaacaagca gactgataac ttccagaagc taattgggtc tctttcttcg gctaagccac
     6541 agtcacaggc ggttggctat gatgccatct ccaactttat aatgcagcaa cagcagtacc
     6601 agcagcagca acagaagcag cacctcatca tacagcagca gcagcaacag accgcctttt
     6661 tggcgagcct gcagctgaag gcgatcctcg gccgggccga cacccaattg ctactgctcc
     6721 gtctgactaa aggggaaata tccaagcacg gattgctggt gcagttggcc aacccacgct
     6781 tgacggacat ggatcgcgag gccatcactg ctgtattgca gttcaccaac acccagcagc
     6841 agcagcagca gcacaagcag cagctggaca tgctctcaag cacggtcatc gccagccagt
     6901 tgcagaatct ccacaacttg gccattgtcc agcagactct tgcagccagg cagcagccgc
     6961 agcataatcc acagacgcag gcgccgcacc aactgtcgca ggaggacttg caagcgcatg
     7021 ctaatgtgat aatgcgaaac gccgtaatga agcgaaaaat ggaggagcag acctcgaagc
     7081 ttatcaatgg cggtgccaag catcaggcac agcagcaata tctaaaccgt ggccagcaac
     7141 gccaggctcg accagatgcc aactcgaatg ccttgctcca tgccttgatc tctggcggtg
     7201 gcaataacca cgcctctggt tatcctatga atggacagcc gcagcagcat cattccaatt
     7261 tgagattcgg tgataaccaa aacttccaat cttttgagtc caaccagccg cacttcgcca
     7321 cgcagtacaa gcagcaatac caacagtctc agcagcaaca tccccatcag cagccccaac
     7381 agctgaattc gcttcatcag aacaacgctg gagctgtcaa tagcttcaat aaggcccaaa
     7441 tgcaagctca atcagctatt tccatgctgc ccaacagcgg ggatgagttt cattaaatcc
     7501 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     7561 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
     7621 aaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga
     7681 tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     7741 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     7801 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     7861 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     7921 atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     7981 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     8041 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     8101 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     8161 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     8221 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     8281 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     8341 caggtagatg acgaccatca gggacagctt caaggccagc aaaaggccag gaaccgtaaa
     8401 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8461 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8521 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8581 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8641 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8701 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8761 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8821 ga
//
