LOCUS       sequence-409570-        5796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Lynch MD, Gill RT
  TITLE     Broad host range vectors for stable genomic library construction.
  JOURNAL   Biotechnol Bioeng. 2006 May 5. 94(1):151-8.
  PUBMED    16496398
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2006 May 5. 94(1):151-8."
COMMENT     Sequence Label: pBMTB-6
FEATURES             Location/Qualifiers
     source          1..5796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..873
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             874..1539
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRRT"
     terminator      complement(4519..4550)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(4578..5456)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYR
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5483..5767
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5690..5709
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc
      481 ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag
      541 tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt
      601 gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat
      661 gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca
      721 ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac
      781 gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca
      841 accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc
      901 attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg
      961 tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg
     1021 cagatgggcc accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga
     1081 cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc
     1141 gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg
     1201 tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat
     1261 cacgacgacc aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg
     1321 tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc
     1381 attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc
     1441 gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg
     1501 gagccgccga cacgggtcac gctgccgcgc cgtacgtaag aggttccaac tttcaccata
     1561 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctagga
     1621 agctaaaatg gaagaacaaa gcgtgaacat ggcgcgattg aagggggagg ttttgcccgc
     1681 cctcttcgcg tcgccggcga cgattggcga gtacggggcc ggcatcgacg gggcggattc
     1741 cctcaacgag ctgtcgaatc tgatggagca cggcgcagtt gccgcgctgg ccgacaaaat
     1801 cagccagatc gtggcgaagc tggccgacgc ggacccccgc aagatcgcgg aaaagcctac
     1861 ctggttcgag aagatgcttg gccgtgaggt tgaacgccag gtgaggtatc aggtcgcccg
     1921 caagacgctc gaccagttgc tggacgaagc cgagggcgta gcgcagcgcg tgcgggacac
     1981 gttgcgcgcc ttggatgaca tgctcaatac gcatgaggcc gaggtagacc ggctcagagc
     2041 ctacattcaa gccgggcgcg agttcctgga cgagaacccc gaggccggcg cggccaaggc
     2101 cggcgtgatc gagttcgaca agccgcgcga acgcttcgcg cgcaagctcg ccaacctggc
     2161 aaccctcatg gcgtcccatg aaatgagcgt cactcagatg aagctcacgc gggcgcaggc
     2221 cgtggacatg ctggaccgct tctctgaaac ggcatccgtc ctggtgcccg tctggcgtca
     2281 gcacaccctc gcgctcatca ccaccaagaa catgaatccg gcaatggtcg ccgaggcggc
     2341 caaagctcac caggcgctca tgcggagcct ttcgcagagc ctggaaggca tcaaccaata
     2401 acacggcggg agaaccctat gaacgcactg aaaacgacgc acgacgccaa ggcccctatc
     2461 gtcgccttcg acatgacccc ggcaaccctg cgcgagctgg gcttgcagga aagcgacgtg
     2521 ccggaagtcc atgcggtcgc gcagcggatc gaggtcggca gtccgcagac cgttgccgag
     2581 ttcggccgcg acgtggccga gcacacgtcc cgctacgccg atagcctgct ggaccaggtg
     2641 cgcaacagcg acctggacga agcaggcgag aaactgaccc aggttgtcgc caaggcccgt
     2701 tccctgaacg tcggcccttt gtccgacaac cgttcccgcc tgcccctgat tggcccgctg
     2761 atcgaccgct cccgcgtccg ttcgacgggc ttcatggcgc gcttcgacac gacccgcgag
     2821 cagatcgaac acctggtcag cgaagtgcag accacccagc aaggcatcgc gcagcgcaat
     2881 gcctcgctcg acgaaatgtt cgcagccgtg cgcgaggaac accgccttct tggcgtccac
     2941 atcgcggccg gcaaggtccg ccttgccgag ctgcgcgagc aggccgaggg tctgcgcggc
     3001 aatgtcggga acgacccggg ccgcgtgcag gagctggccg acctcgatgc gatggttgcc
     3061 aacctggaca agcgcatcgg cgacctgatc gccttgcaac attcggccat gcagagcctg
     3121 ccgaccatcc gcatgatcca ggccaacaac cagatgctgg tcgataaatt ccacaccatc
     3181 cgcgaaatca ccgtgccggc gtggaagcgg caattcatgc tggccttgag cctcaacgag
     3241 cagaagaacg ccgtcgaact ggccacggcc atcgacgaca ccaccaacga cctgatgaag
     3301 cgcaatgcgg ccctgctgca tcgcacgtcc gtcgagacgg cgaaggagaa ccaacgcctg
     3361 gtgatcgacg tggacacgct caagcaggtt cagacgacgc tcatcaagac cgtcgaggac
     3421 gttattcgca tccagcagga aggcgtgcag aagcgcaagg atgccgagaa gcagatcgcc
     3481 gcaatgcgtg gcgatcttca agccaagctg acccgccagc ccgtgcgcga gctggcccaa
     3541 caggagtccg tatgaatgcc acaaacaccg atgttttcgc ccaggtaggc ggcctcgagg
     3601 cccgaggcgc gaagatgaag aagcggggca cccgcttcct catcgcggcg ctggcagtcc
     3661 ttgccattgc cgggatcggg gcagtaacgg gatgggcgat cagcccgagc gcgacgcccg
     3721 gaagcattga cgtgccgcag gtgctggcat cgacattcag cgaccaggtg ccgggcagtg
     3781 agggcggcgg cctgggtggc ggcctgccct tcacttcggc cgtcggggca ttcacggact
     3841 tcatggcggg gccggcaatt tttaccttgg gcattcttgg catagtggtc gcgggtgccg
     3901 tgctcgtgtt cgggggtgaa ttctgcgggt tcgtgcgatc cgtctgcatg atggtgatag
     3961 ccgtcagcat gattttcgtg tcgtcgaact tggtgaaggg cattctcggc ggcgatcacg
     4021 acgccggccc tgcggagcct tcgccgcgtg cgcgattcat ggcggccgtg gaggccaagg
     4081 atttcgcgcg agtgcaagag ctgatcgagg cgcgtggagc caagtcggcg gctgattatg
     4141 tccttgcgca gctcgccgtg gccgaaggtc tggaccgcaa gcctggtgcg cgcgtcgtgg
     4201 tcgggaaagc ggcgggcagc atggcaatgc cgcctgcggc gctgggtttt acgccaaggg
     4261 gagaagcggc atacgccatc gagcggtcag cctatggtga gccgaggtcc agcattgcga
     4321 agcagtacca gcaggaatgg aaccggaagg cggcgacctg gtgggcgatg gccggtgtgg
     4381 ccggcatcat cggcgcgatc ctggcggcgg cggcaaccgg ctttgttggg ctggcagtgt
     4441 cgatccgcaa ccgagtgaag cgcgtgcgcg acctgttggt gatggagccg ggtgcagagc
     4501 cataagggat ccccctcaag tcaaaagcct ccggtcggag gtttttgact ttctgctatg
     4561 gaggtcaggt atgattttta tgacaacttg acggctacat cattcacttt ttcttcacaa
     4621 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
     4681 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
     4741 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
     4801 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
     4861 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
     4921 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
     4981 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
     5041 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
     5101 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccggta ggcgcgcgga
     5161 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
     5221 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga
     5281 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
     5341 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
     5401 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
     5461 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
     5521 cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc
     5581 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
     5641 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
     5701 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
     5761 tctccatacc cgtttttttg ggctagagaa ttcgtc
//
