LOCUS       sequence-409571-       11116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Wauford N, Patel A, Tordoff J, Enghuus C, Jin A, Toppen J, Kemp ML, 
            Weiss R
  TITLE     Synthetic symmetry breaking and programmable multicellular structure
            formation.
  JOURNAL   Cell Syst. 2023 Sep 20;14(9):806-818.e5. doi: 
            10.1016/j.cels.2023.08.001. Epub 2023 Sep 8.
  PUBMED    37689062
REFERENCE   2  (bases 1 to 11116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.08.001"; journalName: "Cell Syst"; date: 
            "2023-09-20- 20"; volume: "14"; issue: "9"; pages: "806-818.e5"
COMMENT     Sequence Label: aCE90
FEATURES             Location/Qualifiers
     source          1..11116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   338..372
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(408..426)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(589..608)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             600..1409
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(667..686)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1564..2152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2053..2072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2306..2323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   2354..2416
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        3742..3786
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    3834..3858
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3899..3908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3905..5719
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             5744..5764
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5955..6003
                     /note="synthetic polyadenylation signal"
     protein_bind    6659..6677
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6694..6712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6730..6748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6766..6784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6801..6819
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6837..6855
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6867..6905
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    6973..6997
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8496..8516
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8707..8755
                     /note="synthetic polyadenylation signal"
     promoter        9385..9596
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          9597..10535
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10492..10512
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      10578..10587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10584..10982
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11035..11083
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gttcgcggct ccagcctaca caatcgctca agacgtgtaa tgcttttatt atatattagt
       61 cacgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
      121 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
      181 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
      241 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
      301 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
      361 atctttctag ggttaaaagc ttggcgtaat catgaattca ggaaagggcc tcgtgatacg
      421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      481 tcggggaaat gtgctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      541 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      601 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
      661 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
      721 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
      781 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
      841 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
      901 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
      961 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     1021 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     1081 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     1141 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     1201 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     1261 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     1321 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     1381 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     1441 agagcattac gctgacttga cgggacggaa gatccttttt gataatctca tgaccaaaat
     1501 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1681 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2161 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2221 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2281 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcag agcgtgccac
     2341 ctggtcgact taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgc
     2401 gtaaaattga cgcatgtgtt ttatcgatct gtatatcgag gtttatttat taatttgaat
     2461 agatattaag ttttattata tttacactta catactaata ataaattcaa caaacaattt
     2521 atttatgttt atttatttat taaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     2581 caaaactttt aaacattctc tcttttacaa aaataaactt attttgtact ttaaaaacag
     2641 tcatgttggt aaaacgacgg ccaggcagta ccggagttct gcaattcatg tgatctgcgc
     2701 ctgggagctc acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     2761 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
     2821 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
     2881 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
     2941 aaaagctagg ctgaaagagg atcttagaat gacacgcgcc tgggagctca cggggacagc
     3001 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
     3061 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
     3121 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
     3181 tgctctttga gcctgcagac acctgggggg atacggggaa aaagctggat cataactcga
     3241 cgtgcatact ggttcctcct ctctctcctt cttacggtat atgatacgaa acgtaccgta
     3301 tcgttaaggt acgccatgat acgaaacgta ccgtatcgtt aaggtaagct atgatacgaa
     3361 acgtaccgta tcgttaaggt gaaggcgagt atatgatacg aaacgtaccg tatcgttaag
     3421 gtacgccatg atacgaaacg taccgtatcg ttaaggtaag ctatgatacg aaacgtaccg
     3481 tatcgttaag gtgaagtgag gtatatgata cgaaacgtac cgtatcgtta aggtacgcca
     3541 tgatacgaaa cgtaccgtat cgttaaggta agctatgata cgaaacgtac cgtatcgtta
     3601 aggtgaagcc tagtatatga tacgaaacgt accgtatcgt taaggtacgc catgatacga
     3661 aacgtaccgt atcgttaagg taagctatga tacgaaacgt accgtatcgt taaggtgaag
     3721 gtaccaacaa aatgtcgtaa caagggcggt aggcgtgtac ggtgggaggt ctatataagc
     3781 agagctccag gtgaaccgtc agatccagat aagtttgtac aaaaaagaga ggtccaagtt
     3841 tgtacaaaaa agcaggctgg atcccggtac cctcgacacc tgctgacagg tgaggcctgc
     3901 caccatggac acctacgccg gcgcttacga tagacagagc agggagcggg aaaacagctc
     3961 tgctgcaagt cctgctacac aacggagcgc caacgaggac aaggccgcag atctgcagag
     4021 ggaggtggag cgtgacgggg gaaggttccg gtttgtcggt cacttcagcg aagcaccggg
     4081 gactagtgcc tttgggaccg ctgagcgccc agaatttgag aggatactga acgaatgcag
     4141 agccggaagg ctgaatatga ttatcgtgta cgacgtcagt cggttttcac gcctgaaggt
     4201 tatggatgca attcccatcg tgtctgaact gctcgcactg ggtgtcacaa ttgtgagcac
     4261 tcaggaaggc gttttcaggc aagggaatgt tatggacctg attcatctga ttatgcgcct
     4321 cgatgcatcc cataaggaat cttcactcaa gagtgcaaaa atcctggata ccaagaatct
     4381 ccagcgtgaa ctggggggct acgtgggagg caaagccccg tacggatttg aactggtcag
     4441 tgagactaaa gaaattactc gaaatggccg aatggtgaac gttgtgataa acaaactggc
     4501 tcacagcact acccctctca caggtccctt cgagtttgaa cctgacgtga ttagatggtg
     4561 gtggagggag atcaagacac ataaacatct gccctttaaa cccggcagcc aggccgctat
     4621 ccacccaggg agtattacag gcctgtgtaa acgaatggac gcagacgccg tcccaacccg
     4681 aggtgaaact attgggaaga aaaccgcaag tagcgcttgg gaccccgcaa ccgtgatgcg
     4741 aatactccga gatcctagga tcgcaggatt tgccgctgag gttatttaca agaagaagcc
     4801 agatggcaca cctactacca aaatcgaagg ttaccggatt caacgcgacc ctatcactct
     4861 gaggcctgtt gaactcgact gcgggcccat tatcgaaccc gccgaatggt acgagctcca
     4921 ggcatggctc gatggccgtg gtaggggcaa ggggctgtct cgcgggcagg ctattctgag
     4981 tgctatggat aaactgtact gtgaatgcgg cgcagttatg acttccaaaa ggggagagga
     5041 aagtattaaa gattcttata gatgtcgacg gcgaaaggtg gtcgacccct ccgctcccgg
     5101 gcaacacgag ggaacttgca acgtgtccat ggctgccctc gataagttcg tggctgaacg
     5161 gatctttaac aagatcagac acgccgaggg cgatgaagag actctggctc tcctgtggga
     5221 ggctgccagg cggttcggta aactgacaga ggcccccgag aagtctgggg agcgcgcaaa
     5281 tctggttgcc gaaagggctg acgcactgaa cgctctggag gagctgtacg aggatagggc
     5341 cgcaggagct tacgacggcc cagtcggtcg aaaacacttt cggaagcagc aggctgcact
     5401 cactctgcgc caacagggtg cagaggaaag actggccgag ctggaagccg cagaggctcc
     5461 caaactgcca ctcgaccagt ggtttcccga ggatgctgat gcagacccca caggccctaa
     5521 gtcctggtgg ggcagggctt cagttgacga taaacgcgtt ttcgttgggc tgtttgtgga
     5581 caaaattgtg gttactaaga gtaccactgg cagaggacaa ggcaccccaa tcgagaaacg
     5641 cgcctctatt acatgggcaa aaccgccaac cgacgatgac gaagatgacg ctcaggacgg
     5701 tactgaggat gtcgccgcat ctagagggcc cgttgccagc ccccccaaga aaaagcggaa
     5761 agtgggatct ggcaagctta gccatggctt cccgccggag gtggaggagc aggatgatgg
     5821 cacgctgccc atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc
     5881 ttctgctagg atcaatgtgt gagcttccac tggattgtac aattaccaac tgatctagaa
     5941 ccggtcatgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     6001 gtgttcgaac tagatgctgt cgaccgatgc gctcgcggag ttctgcaatt catgtgatct
     6061 gcgcctggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
     6121 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
     6181 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
     6241 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
     6301 gggaaaaagc taggctgaaa gaggatctta gaatgacacg cgcctgggag ctcacgggga
     6361 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     6421 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     6481 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     6541 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ggatcataac
     6601 tcgacgtgca tactgctccg aattcgccct tcaggtccga ggttctagac gagtttactc
     6661 cctatcagtg atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt
     6721 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     6781 gagaacgtat gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc
     6841 tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca
     6901 gagctcgttt agtgaaccgt cagatcgcaa agggcgaatt cgaccagata agtttgtaca
     6961 aaaaagagag gtccaagttt gtacaaaaaa gcaggctgga tcccggtacc ctcgacacct
     7021 gctgacaggt gaggcctgcc accatgaaat acgcagtcta cgtcagagtc agcacagata
     7081 gggatgagca agtctcttcc gtcgaaaacc agatcgatat ttgcagatac tggctggaga
     7141 agaacgggta tgaatgggac cccaatgccg tgtactttga cgatggaatc tccggaaccg
     7201 catggctgga gaggcacgct atgcagctga ttctggagaa agcccggaga aatgaactgg
     7261 atacagtggt tttcaagtct atccaccgac tggcacggga cctgagagat gccctggaga
     7321 ttaaggaaat cctgattgga catggcatcc ggctggtgac tattgaggaa aactacgact
     7381 ctctgtatga gggcgggaat gatatcaaat tcgaaatgtt tgccatgttc gccgctcagc
     7441 tgccaaagac cattagcgtg tccgtctctg cagccatgca ggctaaagca aggcgcgggg
     7501 agtttatcgg aaagcccggg ctgggatacg acgtgatcga taagaaactg gtcattaacg
     7561 agaaagaagc tgagatcgtg agagagattt ttgatctgag ctacaagggc tatgggttca
     7621 agaaaatcgc aaacattctg aatgacaaag gaacttatac caagttcggc cagctgtggt
     7681 cacataccac agtgggcaag atcctgaaaa atcagacata caaggggaac ctggtcctga
     7741 atagttataa gactgtgaaa gtcgatggca agaaaaagag agtgtacacc cccaaggaga
     7801 ggctgacaat cattgaggac cactatccta caatcgtgag taaggagctg tggaacgctg
     7861 tcaatagtga tcgcgcatca aaaaagaaaa caaaacagga cactcgaaac gagtttcggg
     7921 ggatgatgtt ctgcaagcat tgtggagaac ctatcactgc caaatacagc ggcaggtatg
     7981 ctaaggggtc caagaaagag tgggtgtaca tgaagtgctc caattatatc aggtttaacc
     8041 gctgcgtgaa tttcgatcct gcacactacg acgatattcg cgaggccatc atctacggac
     8101 tgaagcagca ggaaaaagag ctggaaatcc actttaaccc aaagatgcat cagaaacgga
     8161 atgacaagag taccgagatc aagaaacaga ttaagctgct gaaagtgaag aaagaaaagc
     8221 tgatcgacct gtacgtcgag ggcctgattg ataaagaaat gttttcaaag cgcgacctga
     8281 acttcgagaa tgaaatcaaa gagcaggaac tggccctgct gaagctgaca gaccagaaca
     8341 agcgaaacaa ggaggaaaag aaaattaagg aggctttttc aatgctggac gaggaaaaag
     8401 atatgcatga agtgttcaag actctgatca agaaaattac cctgagcaaa gacaaataca
     8461 ttgacatcga atacaccttc agcctggcca gcccccccaa gaaaaagcgg aaagtgggat
     8521 ctggcaagct tagccatggc ttcccgccgg aggtggagga gcaggatgat ggcacgctgc
     8581 ccatgtcttg tgcccaggag agcgggatgg accgtcaccc tgcagcctgt gcttctgcta
     8641 ggatcaatgt gtgagcttcc actggattgt acaattacca actgatctag aaccggtcat
     8701 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgttcga
     8761 actagatgct gtcgaccgat gggagttctg caattcatgt gatctgcgcc tgggagctca
     8821 cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
     8881 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
     8941 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
     9001 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaagctaggc
     9061 tgaaagagga tcttagaatg acacgcgcct gggagctcac ggggacagcc cccccccaaa
     9121 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
     9181 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
     9241 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
     9301 cctgcagaca cctgggggga tacggggaaa aagctggatc ataactcgac gtgcatactt
     9361 taattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     9421 tgtggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     9481 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     9541 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     9601 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     9661 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     9721 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     9781 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     9841 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     9901 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     9961 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
    10021 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
    10081 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
    10141 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
    10201 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
    10261 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
    10321 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
    10381 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
    10441 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
    10501 agacagtggt tcaaagtttt tttcttccat ttcaggtgga tcccaagggc agataagttt
    10561 gtacaaaaaa gagaggtgcc accatggcca agcctttgtc tcaagaagaa tccaccctca
    10621 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagattac agcgtcgcca
    10681 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
    10741 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
    10801 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
    10861 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
    10921 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
    10981 aagcttccac tggattgtac aattaccaac tgatctagaa ccggtcatgg ccgcaataaa
    11041 atatctttat tttcattaca tccgtgtgtt ggttttttgt gtgttcgaac tagatgctgt
    11101 cgaccgatgc gctaga
//
