LOCUS       sequence-409575-        8999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Figure 1. reporter circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Wauford N, Patel A, Tordoff J, Enghuus C, Jin A, Toppen J, Kemp ML, 
            Weiss R
  TITLE     Synthetic symmetry breaking and programmable multicellular structure
            formation.
  JOURNAL   Cell Syst. 2023 Sep 20;14(9):806-818.e5. doi: 
            10.1016/j.cels.2023.08.001. Epub 2023 Sep 8.
  PUBMED    37689062
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.08.001"; journalName: "Cell Syst"; date: 
            "2023-09-20- 20"; volume: "14"; issue: "9"; pages: "806-818.e5"
COMMENT     Sequence Label: aCE120
FEATURES             Location/Qualifiers
     source          1..8999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..38)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(201..220)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             212..1021
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(279..298)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1176..1764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1665..1684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1892..1912
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1930..1967
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    1977..2001
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2017..2616
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2017..2036)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2513..2533
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(2618..2642)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2732..2751)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2797..2852
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2851..2870)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        3826..4037
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4038..4976
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4933..4953
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5117..5126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5123..5125,5126..5128,5129..5842)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     polyA_signal    5895..5943
                     /note="synthetic polyadenylation signal"
     regulatory      6644..6653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6650..7342
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     polyA_signal    7407..7455
                     /note="synthetic polyadenylation signal"
     protein_bind    8096..8129
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     regulatory      8140..8149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8146..8148,8149..8151,8152..8865)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    8918..8966
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gttcgcaatt caggaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgctggc ccgtgtctca
      121 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      181 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
      241 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
      301 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
      361 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
      421 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      481 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
      541 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
      601 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
      661 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
      721 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
      781 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
      841 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
      901 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
      961 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     1021 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     1081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1801 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggcatgc
     1861 gtatcgatgt cgacgttaac gctagtgata tcaactttgt atagaaaagt tggctccgaa
     1921 tttctcgagg gcttgtcgac gacggcggtc tccgtcgtca ggatcatgaa ttcgacccaa
     1981 gtttgtacaa aaaagcaggc tgaacggcct gccaccatga ccgagtacaa gcccacggtg
     2041 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     2101 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     2161 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     2221 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     2281 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     2341 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     2401 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     2461 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     2521 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     2581 cgcacctggt gcatgacccg caagcccggt gcctgatacc cagctttctt gtacaaagtg
     2641 gtacgcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     2701 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     2761 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     2821 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     2881 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     2941 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     3001 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     3061 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     3121 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     3181 tcgacgagga agcagagcgt aaaacgacgg ccaggcagta ccggagttct gcaattcatg
     3241 tgatctgcgc ctgggagctc acggggacag ccccccccca aagcccccag ggatgtaatt
     3301 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
     3361 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
     3421 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
     3481 gatacgggga aaaagctagg ctgaaagagg atcttagaat gacacgcgcc tgggagctca
     3541 cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
     3601 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
     3661 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
     3721 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaagctggat
     3781 cataactcga cgtgcatact ttaattggct ccggtgcccg tcagtgggca gagcgcacat
     3841 cgcccacagt ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa
     3901 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     3961 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     4021 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     4081 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     4141 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     4201 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     4261 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     4321 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     4381 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     4441 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     4501 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     4561 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     4621 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     4681 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     4741 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     4801 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     4861 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     4921 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgg
     4981 atcccaaggg cagatagttt taaagttggt tattagttac tgtgatattt atcacggtac
     5041 ccaataacca atgaaggagt agtgccccaa ctggggtaac ctttgagttc tctcagttgg
     5101 gggcgtaggg tcaggtgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     5161 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgaggggcga
     5221 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5281 gctgcccgtg ccctggccca ccctcgtgac caccctgagc cacggcgtgc agtgcttcgc
     5341 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5401 cgtccaggag cgcaccatct tcttcaagga cgacggcacc tacaagaccc gcgccgaggt
     5461 gaagttcgag ggcgacaccc tagtgaaccg catcgagctg aagggcgtcg acttcaagga
     5521 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acatctatat
     5581 catggccgtc aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacgtgga
     5641 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5701 cgtgctgctg cccgacagcc actacctgag cacccagtcc gtgctgagca aagaccccaa
     5761 cgagaagcgc gatcacatgg tcctgctgga gttccgcacc gccgccggga tcactctcgg
     5821 catggacgag ctgtacaagt gagcttccac tggattgtac aattaccaac tgatctagaa
     5881 ccggtcatgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     5941 gtgttcgaac tagatgctgt cgaccgatgc gctcgcggag ttctgcaatt catgtgatct
     6001 gcgcctggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
     6061 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
     6121 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
     6181 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
     6241 gggaaaaagc taggctgaaa gaggatctta gaatgacacg cgcctgggag ctcacgggga
     6301 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     6361 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     6421 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     6481 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ggatcataac
     6541 tcgacgtgca tacttaagtt tgtacaaaaa agagcagaaa ggtagcgtca acgataggtg
     6601 taactgtcgt gtttgtaacg gtacttccaa cagctggcga ggtgccacca tggtgagcga
     6661 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
     6721 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     6781 caaggcggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
     6841 gtacggcagc aaaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
     6901 cttccccgag ggcttcacat gggagagagt caccacatac gaagatgggg gcgtgctgac
     6961 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     7021 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctc
     7081 caccgagaca ctgtaccccg ctgacggcgg cctggaaggc agagccgaca tggccctgaa
     7141 gctcgtgggc gggggccacc tgatctgcaa ccttaagacc acatacagat ccaagaaacc
     7201 cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac aggagactgg aaagaatcaa
     7261 ggaggccgac aaagagacat acgtcgagca gcacgaggtg gctgtggcca gatactgcga
     7321 cctccctagc aaactggggc acaaacttaa ttgagcttcc actggattgt acaattacca
     7381 actgatctag aaccggtcat ggccgcaata aaatatcttt attttcatta catctgtgtg
     7441 ttggtttttt gtgtgttcga actagatgct gtcgaccgat gcgcttacgg agttctgcaa
     7501 ttcatgtgat ctgcgcctgg gagctcacgg ggacagcccc cccccaaagc ccccagggat
     7561 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
     7621 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
     7681 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
     7741 tggggggata cggggaaaaa gctaggctga aagaggatct tagaatgaca cgcgcctggg
     7801 agctcacggg gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc
     7861 tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc
     7921 cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc
     7981 tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag
     8041 ctggatcata actcgacgtg catacttaag tttgtacaaa aaagagcaga tgcgggtgcc
     8101 agggcgtgcc cttgggctcc ccgggcgcgt actccaggtg ccaccatggt gagcaagggc
     8161 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     8221 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     8281 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     8341 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     8401 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     8461 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     8521 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     8581 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     8641 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     8701 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     8761 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     8821 accgccgccg ggatcactct cggcatggac gagctgtaca agtgagcttc cactggattg
     8881 tacaattacc aactgatcta gaaccggtca tggccgcaat aaaatatctt tattttcatt
     8941 acatctgtgt gttggttttt tgtgtgttcg aactagatgc tgtcgaccga tgcgctaga
//
