LOCUS       sequence-409577-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Wauford N, Patel A, Tordoff J, Enghuus C, Jin A, Toppen J, Kemp ML, 
            Weiss R
  TITLE     Synthetic symmetry breaking and programmable multicellular structure
            formation.
  JOURNAL   Cell Syst. 2023 Sep 20;14(9):806-818.e5. doi: 
            10.1016/j.cels.2023.08.001. Epub 2023 Sep 8.
  PUBMED    37689062
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.08.001"; journalName: "Cell Syst"; date: 
            "2023-09-20- 20"; volume: "14"; issue: "9"; pages: "806-818.e5"
COMMENT     Sequence Label: aCE89
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   143..205
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1531..1575
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    1623..1647
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1688..1697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1694..3508
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             3533..3553
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3609..3657
                     /note="synthetic polyadenylation signal"
     protein_bind    4313..4331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4348..4366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4384..4402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4420..4438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4455..4473
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4491..4509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4521..4559
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    4627..4651
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6150..6170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6226..6274
                     /note="synthetic polyadenylation signal"
     promoter        6904..7115
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          7116..8054
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8011..8031
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8097..8106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8103..8501
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8554..8602
                     /note="synthetic polyadenylation signal"
     repeat_region   8973..9007
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9043..9061)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9224..9243)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9235..10044
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9302..9321)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10199..10787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10688..10707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcaga
      121 gcgtgccacc tggtcgactt aaccctagaa agataatcat attgtgacgt acgttaaaga
      181 taatcatgcg taaaattgac gcatgtgttt tatcgatctg tatatcgagg tttatttatt
      241 aatttgaata gatattaagt tttattatat ttacacttac atactaataa taaattcaac
      301 aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct
      361 ataaagtaac aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt
      421 taaaaacagt catgttggta aaacgacggc caggcagtac cggagttctg caattcatgt
      481 gatctgcgcc tgggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta
      541 cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc
      601 tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca
      661 cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg
      721 atacggggaa aaagctaggc tgaaagagga tcttagaatg acacgcgcct gggagctcac
      781 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
      841 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
      901 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
      961 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctggatc
     1021 ataactcgac gtgcatactg gttcctcctc tctctccttc ttacggtata tgatacgaaa
     1081 cgtaccgtat cgttaaggta cgccatgata cgaaacgtac cgtatcgtta aggtaagcta
     1141 tgatacgaaa cgtaccgtat cgttaaggtg aaggcgagta tatgatacga aacgtaccgt
     1201 atcgttaagg tacgccatga tacgaaacgt accgtatcgt taaggtaagc tatgatacga
     1261 aacgtaccgt atcgttaagg tgaagtgagg tatatgatac gaaacgtacc gtatcgttaa
     1321 ggtacgccat gatacgaaac gtaccgtatc gttaaggtaa gctatgatac gaaacgtacc
     1381 gtatcgttaa ggtgaagcct agtatatgat acgaaacgta ccgtatcgtt aaggtacgcc
     1441 atgatacgaa acgtaccgta tcgttaaggt aagctatgat acgaaacgta ccgtatcgtt
     1501 aaggtgaagg taccaacaaa atgtcgtaac aagggcggta ggcgtgtacg gtgggaggtc
     1561 tatataagca gagctccagg tgaaccgtca gatccagata agtttgtaca aaaaagagag
     1621 gtccaagttt gtacaaaaaa gcaggctgga tcccggtacc ctcgacacct gctgacaggt
     1681 gaggcctgcc accatggaca cctacgccgg cgcttacgat agacagagca gggagcggga
     1741 aaacagctct gctgcaagtc ctgctacaca acggagcgcc aacgaggaca aggccgcaga
     1801 tctgcagagg gaggtggagc gtgacggggg aaggttccgg tttgtcggtc acttcagcga
     1861 agcaccgggg actagtgcct ttgggaccgc tgagcgccca gaatttgaga ggatactgaa
     1921 cgaatgcaga gccggaaggc tgaatatgat tatcgtgtac gacgtcagtc ggttttcacg
     1981 cctgaaggtt atggatgcaa ttcccatcgt gtctgaactg ctcgcactgg gtgtcacaat
     2041 tgtgagcact caggaaggcg ttttcaggca agggaatgtt atggacctga ttcatctgat
     2101 tatgcgcctc gatgcatccc ataaggaatc ttcactcaag agtgcaaaaa tcctggatac
     2161 caagaatctc cagcgtgaac tggggggcta cgtgggaggc aaagccccgt acggatttga
     2221 actggtcagt gagactaaag aaattactcg aaatggccga atggtgaacg ttgtgataaa
     2281 caaactggct cacagcacta cccctctcac aggtcccttc gagtttgaac ctgacgtgat
     2341 tagatggtgg tggagggaga tcaagacaca taaacatctg ccctttaaac ccggcagcca
     2401 ggccgctatc cacccaggga gtattacagg cctgtgtaaa cgaatggacg cagacgccgt
     2461 cccaacccga ggtgaaacta ttgggaagaa aaccgcaagt agcgcttggg accccgcaac
     2521 cgtgatgcga atactccgag atcctaggat cgcaggattt gccgctgagg ttatttacaa
     2581 gaagaagcca gatggcacac ctactaccaa aatcgaaggt taccggattc aacgcgaccc
     2641 tatcactctg aggcctgttg aactcgactg cgggcccatt atcgaacccg ccgaatggta
     2701 cgagctccag gcatggctcg atggccgtgg taggggcaag gggctgtctc gcgggcaggc
     2761 tattctgagt gctatggata aactgtactg tgaatgcggc gcagttatga cttccaaaag
     2821 gggagaggaa agtattaaag attcttatag atgtcgacgg cgaaaggtgg tcgacccctc
     2881 cgctcccggg caacacgagg gaacttgcaa cgtgtccatg gctgccctcg ataagttcgt
     2941 ggctgaacgg atctttaaca agatcagaca cgccgagggc gatgaagaga ctctggctct
     3001 cctgtgggag gctgccaggc ggttcggtaa actgacagag gcccccgaga agtctgggga
     3061 gcgcgcaaat ctggttgccg aaagggctga cgcactgaac gctctggagg agctgtacga
     3121 ggatagggcc gcaggagctt acgacggccc agtcggtcga aaacactttc ggaagcagca
     3181 ggctgcactc actctgcgcc aacagggtgc agaggaaaga ctggccgagc tggaagccgc
     3241 agaggctccc aaactgccac tcgaccagtg gtttcccgag gatgctgatg cagaccccac
     3301 aggccctaag tcctggtggg gcagggcttc agttgacgat aaacgcgttt tcgttgggct
     3361 gtttgtggac aaaattgtgg ttactaagag taccactggc agaggacaag gcaccccaat
     3421 cgagaaacgc gcctctatta catgggcaaa accgccaacc gacgatgacg aagatgacgc
     3481 tcaggacggt actgaggatg tcgccgcatc tagagggccc gttgccagcc cccccaagaa
     3541 aaagcggaaa gtgtgagctt ccactggatt gtacaattac caactgatct agaaccggtc
     3601 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgttc
     3661 gaactagatg ctgtcgaccg atgcgctcgc ggagttctgc aattcatgtg atctgcgcct
     3721 gggagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc
     3781 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
     3841 ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
     3901 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
     3961 aagctaggct gaaagaggat cttagaatga cacgcgcctg ggagctcacg gggacagccc
     4021 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc
     4081 cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg
     4141 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg
     4201 ctctttgagc ctgcagacac ctggggggat acggggaaaa agctggatca taactcgacg
     4261 tgcatactgc tccgaattcg cccttcaggt ccgaggttct agacgagttt actccctatc
     4321 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     4381 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     4441 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     4501 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
     4561 gtttagtgaa ccgtcagatc gcaaagggcg aattcgacca gataagtttg tacaaaaaag
     4621 agaggtccaa gtttgtacaa aaaagcaggc tggatcccgg taccctcgac acctgctgac
     4681 aggtgaggcc tgccaccatg aaatacgcag tctacgtcag agtcagcaca gatagggatg
     4741 agcaagtctc ttccgtcgaa aaccagatcg atatttgcag atactggctg gagaagaacg
     4801 ggtatgaatg ggaccccaat gccgtgtact ttgacgatgg aatctccgga accgcatggc
     4861 tggagaggca cgctatgcag ctgattctgg agaaagcccg gagaaatgaa ctggatacag
     4921 tggttttcaa gtctatccac cgactggcac gggacctgag agatgccctg gagattaagg
     4981 aaatcctgat tggacatggc atccggctgg tgactattga ggaaaactac gactctctgt
     5041 atgagggcgg gaatgatatc aaattcgaaa tgtttgccat gttcgccgct cagctgccaa
     5101 agaccattag cgtgtccgtc tctgcagcca tgcaggctaa agcaaggcgc ggggagttta
     5161 tcggaaagcc cgggctggga tacgacgtga tcgataagaa actggtcatt aacgagaaag
     5221 aagctgagat cgtgagagag atttttgatc tgagctacaa gggctatggg ttcaagaaaa
     5281 tcgcaaacat tctgaatgac aaaggaactt ataccaagtt cggccagctg tggtcacata
     5341 ccacagtggg caagatcctg aaaaatcaga catacaaggg gaacctggtc ctgaatagtt
     5401 ataagactgt gaaagtcgat ggcaagaaaa agagagtgta cacccccaag gagaggctga
     5461 caatcattga ggaccactat cctacaatcg tgagtaagga gctgtggaac gctgtcaata
     5521 gtgatcgcgc atcaaaaaag aaaacaaaac aggacactcg aaacgagttt cgggggatga
     5581 tgttctgcaa gcattgtgga gaacctatca ctgccaaata cagcggcagg tatgctaagg
     5641 ggtccaagaa agagtgggtg tacatgaagt gctccaatta tatcaggttt aaccgctgcg
     5701 tgaatttcga tcctgcacac tacgacgata ttcgcgaggc catcatctac ggactgaagc
     5761 agcaggaaaa agagctggaa atccacttta acccaaagat gcatcagaaa cggaatgaca
     5821 agagtaccga gatcaagaaa cagattaagc tgctgaaagt gaagaaagaa aagctgatcg
     5881 acctgtacgt cgagggcctg attgataaag aaatgttttc aaagcgcgac ctgaacttcg
     5941 agaatgaaat caaagagcag gaactggccc tgctgaagct gacagaccag aacaagcgaa
     6001 acaaggagga aaagaaaatt aaggaggctt tttcaatgct ggacgaggaa aaagatatgc
     6061 atgaagtgtt caagactctg atcaagaaaa ttaccctgag caaagacaaa tacattgaca
     6121 tcgaatacac cttcagcctg gccagccccc ccaagaaaaa gcggaaagtg tgagcttcca
     6181 ctggattgta caattaccaa ctgatctaga accggtcatg gccgcaataa aatatcttta
     6241 ttttcattac atctgtgtgt tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg
     6301 ggagttctgc aattcatgtg atctgcgcct gggagctcac ggggacagcc cccccccaaa
     6361 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
     6421 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
     6481 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
     6541 cctgcagaca cctgggggga tacggggaaa aagctaggct gaaagaggat cttagaatga
     6601 cacgcgcctg ggagctcacg gggacagccc ccccccaaag cccccaggga tgtaattacg
     6661 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
     6721 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
     6781 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
     6841 acggggaaaa agctggatca taactcgacg tgcatacttt aattggctcc ggtgcccgtc
     6901 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt gtggggaggg gtcggcaatt
     6961 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     7021 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     7081 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     7141 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     7201 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     7261 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     7321 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     7381 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     7441 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     7501 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     7561 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     7621 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     7681 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     7741 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     7801 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     7861 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     7921 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     7981 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     8041 ttcttccatt tcaggtggat cccaagggca gataagtttg tacaaaaaag agaggtgcca
     8101 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     8161 tcaacagcat ccccatctct gaagattaca gcgtcgccag cgcagctctc tctagcgacg
     8221 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     8281 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     8341 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     8401 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     8461 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcttccact ggattgtaca
     8521 attaccaact gatctagaac cggtcatggc cgcaataaaa tatctttatt ttcattacat
     8581 ctgtgtgttg gttttttgtg tgttcgaact agatgctgtc gaccgatgcg ctagagttcg
     8641 cggctccagc ctacacaatc gctcaagacg tgtaatgctt ttattatata ttagtcacga
     8701 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     8761 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     8821 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     8881 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     8941 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     9001 tctagggtta aaagcttggc gtaatcatga attcaggaaa gggcctcgtg atacgcctat
     9061 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     9121 gaaatgtgct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     9181 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     9241 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     9301 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     9361 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     9421 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     9481 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     9541 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     9601 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     9661 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     9721 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     9781 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     9841 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     9901 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     9961 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
    10021 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
    10081 attacgctga cttgacggga cggaagatcc tttttgataa tctcatgacc aaaatccctt
    10141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10321 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10621 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacat
//
