LOCUS       sequence-409580-        9189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expressing BA.2.86 SARS-CoV-2 Spike,
            with 19AA cytoplasmic tail truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9189)
  TITLE     SARS-CoV-2 PSV spike variants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: BA.2.86
FEATURES             Location/Qualifiers
     source          1..9189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4780..4797)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4786..5010
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5056..5484
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5143..5162)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5353..5374
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5493..5513)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5498..5827
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5678..5813
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5894..6688
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5948..5967)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6558..6577
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6862..6983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6899..6918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6953..6972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7032..7048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7032..7048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7045..7067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7056..7072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7080..7086,7087..7104,7105..7110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7125..7146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7263..7280)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7434..8022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7514..7533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8193..8984,8985..9053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8816..8835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9054..9158)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg ccaccatgtt cgtgtttctg gtgctgctgc ctctggtgtc
      961 cagccagtgt gtgatgccac tgtttaacct gatcaccact acacagtcgt acaccaacag
     1021 ctttaccaga ggcgtgtact accccgacaa ggtgttcaga tccagcgtgc tgcacctgac
     1081 ccaggacctg ttcctgcctt tcttcagcaa cgtgacctgg ttccacgcca tctccggcac
     1141 caatggcacc aagagattcg acaaccccgt tctgcccttc aacgacgggg tgtactttgc
     1201 cagcaccgag aagtccaaca tcatcagagg ctggatcttc ggcaccacac tggacagcaa
     1261 gacccagagc ctgctgatcg tgaacaacgc caccaacgtg tttatcaaag tgtgcgagtt
     1321 ccagttctgc aacgacccct tcctggacgt ctaccacaag aacaacaaga gctggatgga
     1381 aagcgagtca ggcgtgtaca gcagcgccaa caactgcacc ttcgagtacg tgtcccagcc
     1441 tttcctgatg gacctggaag gcaagcaggg caacttcaag aacctgcgcg agttcgtgtt
     1501 caagaacatc gacggctact tcaagatcta cagcaagcac acccctatca tcggccggga
     1561 ttttcctcag ggcttctctg ctctggaacc cctggtggat ctgcccatcg gcatcaacat
     1621 cacccggttt cagacactgc tggccctgaa tagaagctac ctgacacctg gtgatagcag
     1681 cagcggatgg acagctggtg ccgccgatta ctatgtgggc tacctgcagc ctagaacctt
     1741 cctgctgaag tacaacgaga acggcaccat caccgacgcc gtggattgtg cccttgatcc
     1801 tctgagcgag acaaagtgca ccctgaagtc cttcaccgtg gaaaagggca tctaccagac
     1861 cagcaacttc cgggtgcagc ccaccgaatc catcgtgcgg ttccccaatg tgaccaatct
     1921 gtgccccttc cacgaggtgt tcaatgccac caggttcgcc tctgtgtacg cctggaaccg
     1981 gactcggatc agcaattgcg tggccgacta ctccgtgctg tacaacttcg cccccttctt
     2041 cgccttcaag tgctacggcg tgtcccctac caagctgaac gacctgtgct tcacaaacgt
     2101 gtacgccgac agcttcgtga tcaaaggaaa tgaagtgagt cagattgccc ctggacagac
     2161 aggcaacatc gccgactaca actacaagct gcccgacgac ttcaccggct gtgtgattgc
     2221 ctggaacagc aacaagctgg actccaaaca tagcggcaac tacgattact ggtaccggct
     2281 gttccggaag tccaagctga agcccttcga gcgggacatc tccaccgaga tctatcaggc
     2341 cggcaacaag ccttgtaaag gcaagggccc caactgctac tttccactgc agtcctacgg
     2401 ctttcggccc acatatggcg tgggccatca gccctacaga gtggtggtgc tgagcttcga
     2461 actgctgcat gcccctgcca cagtgtgcgg ccctaagaaa agcaccaatc tcgtgaagaa
     2521 caaatgcgtg aacttcaact tcaacggcct gaccggcaca ggcgtgctga caaaaagcaa
     2581 caagaagttc ctgccattcc agcagtttgg ccgggatatc gtcgatacca cagacgccgt
     2641 tagagatccc cagacactgg aaatcctgga catcacccct tgcagcttcg gcggagtgtc
     2701 tgtgatcacc cctggcacca acaccagcaa tcaggtggca gtgctgtacc agggcgtgaa
     2761 ctgtaccgaa gtgagcgtgg ccattcacgc cgatcagctg acacctacat ggcgggtgta
     2821 ctccaccggc agcaatgtgt ttcagaccag agccggctgt ctgatcggag ccgagtacgt
     2881 gaacaatagc tacgagtgcg acatccccat cggcgctggc gtgtgtgcca gctaccagac
     2941 acagacaaag agccgcagac gggccagatc tgtggccagc cagagcatca ttgcctacac
     3001 aatgtctctg ggcgccgaga acagcgtggc ctactccaac aactctatcg ctatccccac
     3061 caacttcacc atcagcgtga ccacagagat cctgcctgtg tccatgacca agaccagcgt
     3121 ggactgcacc atgtacatct gcggcgattc caccgagtgc tccaacctgc tgctgcagta
     3181 cggcagcttc tgcacccagc tgaaaagagc cctgacaggg atcgccgtgg aacaggacaa
     3241 gaacacccaa gaggtgttcg cccaagtgaa gcagatctac aagacccctc ctatcaagta
     3301 cttcggcggc ttcaatttca gccagattct gcccgatcct agcaagccca gcaagcggag
     3361 cttcatcgag gacctgctgt tcaacaaagt gacactggcc gacgccggct tcatcaagca
     3421 gtatggcgat tgtctgggcg acattgccgc cagggatctg atttgcgccc agaagtttaa
     3481 cggactgaca gtgctgcctc ctctgctgac cgatgagatg atcgcccagt acacatctgc
     3541 cctgctggcc ggcacaatca caagcggctg gacatttgga gctggcgccg ctctgcagat
     3601 cccctttgct atgcagatgg cctacagatt caacggcatc ggagtgaccc agaatgtgct
     3661 gtacgagaac cagaagctga tcgccaacca gttcaacagc gccatcggca agatccagga
     3721 cagcctgttc agcacagcaa gcgccctggg aaagctgcag gacgtggtca accacaatgc
     3781 ccaggcactg aacaccctgg tcaagcagct gtcctccaag ttcggcgcca tcagctctgt
     3841 gctgaacgat atcctgagca gactggacaa ggtggaggcc gaggtgcaga tcgacagact
     3901 gatcacaggc agactgcaga gcctccagac atacgtgacc cagcagctga tcagagccgc
     3961 cgagattaga gcctctgcca atctggccgc caccaagatg tctgagtgtg tgctgggcca
     4021 gagcaagaga gtggactttt gcggcaaggg ctaccacctg atgagcttcc ctcagtctgc
     4081 ccctcacggc gtggtgtttc tgcacgtgac atacgttccc gctcaagaga agaatttcac
     4141 caccgctcca gccatctgcc acgacggcaa agcccacttt cctagagaag gcgtgttcgt
     4201 gtccaacggc acccattggt tcgtgacaca gcggaacttc tacgagcccc agatcatcac
     4261 caccgacaac accttcgtgt ctggcaactg cgacgtcgtg atcggcattg tgaacaatac
     4321 cgtgtacgac cctctgcagt tggagctgga cagcttcaaa gaggaactgg acaagtactt
     4381 taagaaccac acaagccccg acgtggacct gggcgatatc agcggaatca atgccagcgt
     4441 cgtgaacatc cagaaagaga tcgaccggct gaacgaggtg gccaagaatc tgaacgagag
     4501 cctgatcgac ctgcaagaac tggggaagta cgagcagtac atcaagtggc cctggtacat
     4561 ctggctgggc tttatcgccg gactgattgc catcgtgatg gtcacaatca tgctgtgttg
     4621 catgaccagc tgctgtagct gcctgaaggg ctgttgtagc tgtggcagct gctgctgatt
     4681 cgacgaggac gattctgagc ccgtgctgaa gggcgtgaaa ctgcactaca catgagcggc
     4741 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4801 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4861 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4921 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4981 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     5041 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5101 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5161 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5221 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5281 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5341 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5401 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5461 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     5521 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5581 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5641 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5701 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5761 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5821 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     5881 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5941 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     6001 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     6061 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     6121 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     6181 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6241 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6301 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6361 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6421 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6481 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6541 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6601 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6661 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6721 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6781 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6841 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6901 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6961 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7261 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7321 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7981 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8041 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8101 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8161 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8221 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8281 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8341 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8401 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8461 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8521 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8581 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8641 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8701 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8761 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8821 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8881 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8941 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9001 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9061 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9121 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9181 cctgacgtc
//
