LOCUS       sequence-409583-        9192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expressing DV.7.1 SARS-CoV-2 Spike, 
            with 19AA cytoplasmic tail truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9192)
  TITLE     SARS-CoV-2 PSV spike variants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: DV.7.1
FEATURES             Location/Qualifiers
     source          1..9192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4783..4800)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4789..5013
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5059..5487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5146..5165)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5356..5377
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5496..5516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5501..5830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5681..5816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5897..6691
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5951..5970)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6561..6580
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6865..6986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6902..6921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6956..6975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7035..7051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7035..7051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7048..7070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7059..7075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7083..7089,7090..7107,7108..7113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7128..7149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7266..7283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7437..8025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7517..7536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8196..8987,8988..9056))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8819..8838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9057..9161)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg ccaccatgtt cgtgtttctg gtgctgctgc ctctggtgtc
      961 cagccagtgt gtgaacctga tcaccagaac acagtcgtac accaacagct ttaccagagg
     1021 cgtgtactac cccgacaagg tgttcagatc cagcgtgctg cactctaccc aggacctgtt
     1081 cctgcctttc ttcagcaacg tgacctggtt ccacgccatc cacgtgtccg gcaccaatgg
     1141 caccaagaga ttcgacaacc ccgttctgcc cttcaacgac ggggtgtact ttgccagcac
     1201 cgagaagtcc aacatcatca gaggctggat cttcggcacc acactggaca gcaagaccca
     1261 gagcctgctg atcgtgaaca acgccaccaa cgtggtcatc aaagtgtgcg agttccagtt
     1321 ctgcaacgac cccttcctgg acgtctatta ccacgagaac aacaagagca ggatggaaag
     1381 cgagcttcgg gtgtacagca gcgccaacaa ctgcaccttc gagtacgtgt cccagccttt
     1441 cctgatggac ctggaaggca agcagggcga cttcaagaac ctgcgcgagt tcgtgttcaa
     1501 gaacatcgac ggctacttca agatctacag caagcacacc cctgtgaacc tcgggcggga
     1561 tctgcctcag ggcttctctg ctctggaacc cctggtggat ctgcccatcg gcatcaacat
     1621 cacccggttt cagacactgc tggccctgca cagaagctac ctgacacctg gagatagcag
     1681 cagcagttgg acagctggtg ccgccgctta ctatgtgggc tacctgcagc ctagaacctt
     1741 cctgctgaag tacaacgaga acggcaccat caccgacgcc gtggattgtg cccttgatcc
     1801 tctgagcgag acaaagtgca ccctgaagtc cttcaccgtg gaaaagggca tctaccagac
     1861 cagcaacttc cgggtgcagc ccaccgaatc catcgtgcgg ttccccaata tcaccaatct
     1921 gtgccccttc cacgaggtgt tcaatgccac cacattcgcc tctgtgtacg cctggaaccg
     1981 gaagcggatc agcaattgcg tggccgacta ctccgtgctg tacaacttcg cccccttctt
     2041 cgccttcaag tgctacggcg tgtcccctac caagctgaac gacctgtgct tcacaaacgt
     2101 gtacgccgac agcttcgtga tccggggaaa tgaagtgagt cagattgccc ctggacagac
     2161 aggcaacatc gccgactaca actacaagct gcccgacgac ttcaccggct gtgtgattgc
     2221 ctggaacagc aacaagctgg actccacggt gagcggcaac tacaattaca ggtaccggtt
     2281 cttacggaag tccaagctga agcccttcga gcgggacatc tccaccgaga tctatcaggc
     2341 cggcaacaag ccttgtaacg gcgtggcagg cagtaactgc tactttccac tgcagtccta
     2401 cggctttcgg cccacatatg gcgtgggcca tcagccctac agagtggtgg tgctgagctt
     2461 cgaactgctg catgcccctg ccacagtgtg cggccctaag aaaagcacca atctcgtgaa
     2521 gaacaaatgc gtgaacttca acttcaacgg cctgaccggc acaggcgtgc tgacagagag
     2581 caacaagaag ttcctgccat tccagcagtt tggccgggat atcgccgata ccacagacgc
     2641 cgttagagat ccccagacac tggaaatcct ggacatcacc ccttgcagct tcggcggagt
     2701 gtctgtgatc acccctggca ccaacaccag caatcaggtg gcagtgctgt accagggcgt
     2761 gaactgtacc gaagtgcccg tggccattca cgccgatcag ctgacaccta catggcgggt
     2821 gtactccacc ggcagcaatg tgtttcagac cagagccggc tgtctgatcg gagccgagta
     2881 cgtgaacaat agctacgagt gcgacatccc catcggcgct ggcatctgtg ccagctacca
     2941 gacacagaca aagagccaca gacgggccag atctgtggcc agccagagca tcattgccta
     3001 cacaatgtct ctgggcgccg agaacagcgt ggcctactcc aacaactcta tcgctatccc
     3061 caccaacttc accatcagcg tgaccacaga gatcctgcct gtgtccatga ccaagaccag
     3121 cgtggactgc accatgtaca tctgcggcga ttccaccgag tgctccaacc tgctgctgca
     3181 gtacggcagc ttctgcaccc agctgaaaag agccctgaca gggatcgccg tggaacagga
     3241 caagaacacc caagaggtgt tcgcccaagt gaagcagatc tacaagaccc ctcctatcaa
     3301 gtacttcggc ggcttcaatt tcagccagat tctgcccgat cctagcaagc ccagcaagcg
     3361 gagcttcatc gaggacctgc tgttcaacaa agtgacactg gccgacgccg gcttcatcaa
     3421 gcagtatggc gattgtctgg gcgacattgc cgccagggat ctgatttgcg cccagaagtt
     3481 taacggaatc acagtgctgc ctcctctgct gaccgatgag atgatcgccc agtacacatc
     3541 tgccctgctg gccggcacaa tcacaagcgg ctggacattt ggagctggcg ccgctctgca
     3601 gatccccttt gctatgcaga tggcctacag attcaacggc atcggagtga cccagaatgt
     3661 gctgtacgag aaccagaagc tgatcgccaa ccagttcaac agcgccatcg gcaagatcca
     3721 ggacagcctg agcagcacag caagcgccct gggaaagctg caggacgtgg tcaaccacaa
     3781 tgcccaggca ctgaacaccc tggtcaagca gctgtcctcc aagttcggcg ccatcagctc
     3841 tgtgctgaac gatatcctga gcagactgga caaggtggag gccgaggtgc agatcgacag
     3901 actgatcaca ggcagactgc agagcctcca gacatacgtg acccagcagc tgatcagagc
     3961 cgccgagatt agagcctctg ccaatctggc cgccaccaag atgtctgagt gtgtgctggg
     4021 ccagagcaag agagtggact tttgcggcaa gggctaccac ctgatgagct tccctcagtc
     4081 tgcccctcac ggcgtggtgt ttctgcacgt gacatacgtt cccgctcaag agaagaattt
     4141 caccaccgct ccagccatct gccacgacgg caaagcccac tttcctagag aaggcgtgtt
     4201 cgtgtccaac ggcacccatt ggttcgtgac acagcggaac ttctacgagc cccagatcat
     4261 caccaccgac aacaccttcg tgtctggcaa ctgcgacgtc gtgatcggca ttgtgaacaa
     4321 taccgtgtac gaccctctgc agcccgagct ggacagcttc aaagaggaac tggacaagta
     4381 ctttaagaac cacacaagcc ccgacgtgga cctgggcgat atcagcggaa tcaatgccag
     4441 cgtcgtgaac atccagaaag agatcgaccg gctgaacgag gtggccaaga atctgaacga
     4501 gagcctgatc gacctgcaag aactggggaa gtacgagcag tacatcaagt ggccctggta
     4561 catctggctg ggctttatcg ccggactgat tgccatcgtg atggtcacaa tcatgctgtg
     4621 ttgcatgacc agctgctgta gctgcctgaa gggctgttgt agctgtggca gctgctgctg
     4681 attcgacgag gacgattctg agcccgtgct gaagggcgtg aaactgcact acacatgagt
     4741 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4801 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4861 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4921 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4981 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     5041 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5101 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5161 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5221 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5281 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5341 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5401 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5461 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     5521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5581 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5761 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5821 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     5881 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5941 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6001 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6061 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6121 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6181 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6241 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6301 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6361 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6421 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6481 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6541 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6601 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6661 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6721 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6781 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6841 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6901 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6961 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7021 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7081 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7201 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7381 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7441 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7501 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7561 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7621 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7681 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7741 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7801 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7861 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7921 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7981 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8041 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8101 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8161 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8221 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8281 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8341 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8401 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8461 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8521 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8581 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8641 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8701 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8761 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8821 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8881 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8941 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9001 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9061 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9121 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9181 ccacctgacg tc
//
