LOCUS       sequence-409587-        9955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoted phiC31 recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Wauford N, Patel A, Tordoff J, Enghuus C, Jin A, Toppen J, Kemp ML, 
            Weiss R
  TITLE     Synthetic symmetry breaking and programmable multicellular structure
            formation.
  JOURNAL   Cell Syst. 2023 Sep 20;14(9):806-818.e5. doi: 
            10.1016/j.cels.2023.08.001. Epub 2023 Sep 8.
  PUBMED    37689062
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.08.001"; journalName: "Cell Syst"; date: 
            "2023-09-20- 20"; volume: "14"; issue: "9"; pages: "806-818.e5"
COMMENT     Sequence Label: pJT88
FEATURES             Location/Qualifiers
     source          1..9955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        366..641
                     /label=chicken beta-actin promoter
     intron          642..1661
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1585..1607
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1669..1688
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1720..1744
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1749..1758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1755..3569
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     protein_bind    complement(3589..3613)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3703..3722)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3768..3823
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3822..3841)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4184..4200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4208..4224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4232..4238,4239..4256,4257..4262))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4277..4298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4471..5307
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5337..5357)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5337..5355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5376..5392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5400..5416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5424..5430,5431..5448,5449..5454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5469..5490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5607..5624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5778..6366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5858..5877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6537..7328,7329..7397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7160..7179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7398..7502)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7528..7983)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7665..7686)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7877..7896
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8125..8141
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8151..8169
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8184..8987
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9066..9091
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    9763..9783
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     9865..9881
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9888..9906
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      421 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      481 gggggcgcgc gccrggsggg gsgggggggg gsggggggsg gggsggggsg aggcggagag
      541 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      601 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgag tcgctgcgtt
      661 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      721 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      781 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
      841 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
      901 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
      961 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     1021 gcgggggggg ctgcgagggg aacaaaggct gcgggcgggg tgtgtgcgtg ggggggtgag
     1081 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     1141 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     1201 tgccgggcgg ggggtggcgg caggkggggg tgccgggcgg ggcggggccg cctcgggccg
     1261 gggrgggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     1321 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1381 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1441 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1501 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     1561 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     1621 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     1681 ttattgtgct gtctcatcat tttggcaaag aattcgaccc caagtttgta caaaaaagca
     1741 ggaggcctgc caccatggac acctacgccg gcgcttacga tagacagagc agggagcggg
     1801 aaaacagctc tgctgcaagt cctgctacac aacggagcgc caacgaggac aaggccgcag
     1861 atctgcagag ggaggtggag cgtgacgggg gaaggttccg gtttgtcggt cacttcagcg
     1921 aagcaccggg gactagtgcc tttgggaccg ctgagcgccc agaatttgag aggatactga
     1981 acgaatgcag agccggaagg ctgaatatga ttatcgtgta cgacgtcagt cggttttcac
     2041 gcctgaaggt tatggatgca attcccatcg tgtctgaact gctcgcactg ggtgtcacaa
     2101 ttgtgagcac tcaggaaggc gttttcaggc aagggaatgt tatggacctg attcatctga
     2161 ttatgcgcct cgatgcatcc cataaggaat cttcactcaa gagtgcaaaa atcctggata
     2221 ccaagaatct ccagcgtgaa ctggggggct acgtgggagg caaagccccg tacggatttg
     2281 aactggtcag tgagactaaa gaaattactc gaaatggccg aatggtgaac gttgtgataa
     2341 acaaactggc tcacagcact acccctctca caggtccctt cgagtttgaa cctgacgtga
     2401 ttagatggtg gtggagggag atcaagacac ataaacatct gccctttaaa cccggcagcc
     2461 aggccgctat ccacccaggg agtattacag gcctgtgtaa acgaatggac gcagacgccg
     2521 tcccaacccg aggtgaaact attgggaaga aaaccgcaag tagcgcttgg gaccccgcaa
     2581 ccgtgatgcg aatactccga gatcctagga tcgcaggatt tgccgctgag gttatttaca
     2641 agaagaagcc agatggcaca cctactacca aaatcgaagg ttaccggatt caacgcgacc
     2701 ctatcactct gaggcctgtt gaactcgact gcgggcccat tatcgaaccc gccgaatggt
     2761 acgagctcca ggcatggctc gatggccgtg gtaggggcaa ggggctgtct cgcgggcagg
     2821 ctattctgag tgctatggat aaactgtact gtgaatgcgg cgcagttatg acttccaaaa
     2881 ggggagagga aagtattaaa gattcttata gatgtcgacg gcgaaaggtg gtcgacccct
     2941 ccgctcccgg gcaacacgag ggaacttgca acgtgtccat ggctgccctc gataagttcg
     3001 tggctgaacg gatctttaac aagatcagac acgccgaggg cgatgaagag actctggctc
     3061 tcctgtggga ggctgccagg cggttcggta aactgacaga ggcccccgag aagtctgggg
     3121 agcgcgcaaa tctggttgcc gaaagggctg acgcactgaa cgctctggaa gagctgtacg
     3181 aggatagggc cgcaggagct tacgacggcc cagtcggtcg aaaacacttt cggaagcagc
     3241 aggctgcact cactctgcgc caacagggtg cagaggaaag actggccgag ctggaagccg
     3301 cagaggctcc caaactgcca ctcgaccagt ggtttcccga ggatgctgat gcagacccca
     3361 caggccctaa gtcctggtgg ggcagggctt cagttgacga taaacgcgtt ttcgttgggc
     3421 tgtttgtgga caaaattgtg gttactaaga gtaccactgg cagaggacaa ggcaccccaa
     3481 tcgagaaacg cgcctctatt acatgggcaa aaccgccaac cgacgatgac gaagatgacg
     3541 ctcaggacgg tactgaggat gtcgccgcat ctagagggcc cgtttaatac ccagctttct
     3601 tgtacaaagt ggtacgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     3661 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     3721 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     3781 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     3841 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     3901 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     3961 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     4021 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     4081 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     4141 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     4201 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4261 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4321 ctttccagtc gggaaacctg tcgtgccagc ggatcgacag tactaagctt ggtgcgtttt
     4381 tatgcttgta gtattgtata atgtttttaa gatccttaat taatagggat aacagggtaa
     4441 tctcgaggtt taaacgtcga cgcggccgct actagggaca ggattggtga cagaaaagcc
     4501 ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc cacctcctgt
     4561 taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc tctccttgcc
     4621 agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg agagcttggc
     4681 agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg ccaccctgcg
     4741 ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt tcactgatcc
     4801 tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa acaaaatcag
     4861 aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc aatttatatt
     4921 gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc cgtcctggca
     4981 gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga atggtgcgtc
     5041 ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca tccttctttc
     5101 cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag ggtcccgctt
     5161 ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag gagaggcgct
     5221 caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct cctgcccctt
     5281 ccctacaggg gttcctggct ctgctctcca ccgcggtgga gctccagctt ttgttccctt
     5341 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6541 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6601 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6661 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6721 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6781 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6841 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6901 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6961 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7021 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7081 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7141 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7201 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7261 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7321 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7381 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7501 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     7561 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     7621 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     7681 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     7741 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     7801 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     7861 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7921 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7981 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     8041 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     8101 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     8161 cactataggg cgaattgggt acctgctttc tctgaccagc attctctccc ctgggcctgt
     8221 gccgctttct gtctgcagct tgtggcctgg gtcacctcta cggctggccc agatccttcc
     8281 ctgccgcctc cttcaggttc cgtcttcctc cactccctct tccccttgct ctctgctgtg
     8341 ttgctgccca aggatgctct ttccggagca cttccttctc ggcgctgcac cacgtgatgt
     8401 cctctgagcg gatcctcccc gtgtctgggt cctctccggg catctctcct ccctcaccca
     8461 accccatgcc gtcttcactc gctgggttcc cttttccttc tccttctggg gcctgtgcca
     8521 tctctcgttt cttaggatgg ccttctccga cggatgtctc ccttgcgtcc cgcctcccct
     8581 tcttgtaggc ctgcatcatc accgtttttc tggacaaccc caaagtaccc cgtctccctg
     8641 gctttagcca cctctccatc ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga
     8701 ttcgggtcac ctctcactcc tttcatttgg gcagctcccc tacccccctt acctctctag
     8761 tctgtgctag ctcttccagc cccctgtcat ggcatcttcc aggggtccga gagctcagct
     8821 agtcttcttc ctccaacccg ggcccctatg tccacttcag gacagcatgt ttgctgcctc
     8881 cagggatcct gtgtccccga gctgggacca ccttatattc ccagggccgg ttaatgtggc
     8941 tctggttctg ggtactttta tctgtcccct ccaccccaca gtggggcaag cttattaccc
     9001 tgttatccct aaccggttta ccgagctctt attggttttc aaacttcatt gactgtgcca
     9061 agcttctgac ctcttctctt cctcccacag ggcctcgaga caattgcatc gatggtacca
     9121 gtgaaagtct gcaatgaatt cgagttggcg cgcctgtcat tctaaatctc tctttcagcc
     9181 taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     9241 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     9301 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     9361 ccctagcggg ggagggacgt aattacatcc ctgggggctt tgggaggggg ctgtccccgt
     9421 gagctcccag gcgcgcctgt cattctaaat ctctctttca gcctaaagct ttttccccgt
     9481 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
     9541 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     9601 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     9661 cgtaattaca tccctggggg ctttgggggg gggctgtccc cgtgagctcc caggcgcgcc
     9721 aactcggtac cgtatcgatg tcgacgttaa cgctagtgat atcaactttg tatagaaaag
     9781 ttggctccga atttctcgag ttaattaaat gtgctgcaag gcgattaagt tgggtaacgc
     9841 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
     9901 tatagggcga attggagcta ggcctacgta gcgcgcgagc tccaccgcgg tggac
//
