LOCUS       sequence-409589-        3281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T3O promoter and native 5'UTR (approximately 1 kb upstream of start 
            codon) from C. roseus in a MoClo compatible L0 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pUN1243 - pL0_pT3O (pro + 5U)
FEATURES             Location/Qualifiers
     source          1..3281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             127..918
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1089..1677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1578..1597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1831..1848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2996..3014)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3082..3186
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
       61 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      121 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      241 gatggcggcc tgaagccaca cagcgatatt gatttgctgg ttacggtgac cgtaaggctt
      301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      841 ttggcctcgc gcgcagatca gttggaagaa tttgtccatt acgtaaaagg cgagatcacc
      901 aaggtagtcg gcaaataact gtcagaccaa gtttactcat atatacttta gattgattta
      961 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1021 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1201 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1441 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1501 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1741 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1801 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1861 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt
     1921 ctcaggagta ttatggaggt taacaaatat ttaccgtaga ttaaaatttt ggccatgaat
     1981 atatcatttc ccttacatta cctctggttc atattataaa atgaaattgt catacttgaa
     2041 gtttcatatt ataatacaat ttccctttat aaatttttaa gaatacccct atgcatttaa
     2101 tgtattacta tcttataatc ttcaaaacct aaatctattc atatttgata aaattttgaa
     2161 aattagatat aggagagaga agagaggaga gaagataaaa attttaaaag atggatttca
     2221 taaaattaat gtaaaggtat taaggtattt tacaagaaac tgaggtagta ttatcacatg
     2281 ctttcatatg tgtgtattag gttttgaaac accaaaattg tgtgttacat ttcctataac
     2341 atctctaata atatttcctt tcataatata ttttttcctg cacaattatc tttttctctt
     2401 atgtttatat tttactaagt tttattaata taaaatataa aatcaggaat attatcgaga
     2461 gcgttatagg agaaccgttt tacaatgaag agctaaacaa acaaaaaatt cttttatgaa
     2521 ctcacttcaa atttctattt atagactata taagttatat taaattacgt cacttttgtt
     2581 ttcttttttt catcttctgt agagaaatcc aaatcataag cacttgatca tacttttatt
     2641 atttaataaa aaaattatat tacaaaatat attctcaata atttcacacc taatcttacg
     2701 acactctgtc actcacttag atcactcatt gaactacagg atccattgca aaattaagga
     2761 gagagacaga aggtgggtga actctcttaa catctcaatt gcataattaa ggagagaggc
     2821 agaaggtggg tgaactccat ggtacatgtg tgggatatat atatatatat atactattca
     2881 gctttatata ttacttggag cagctaccat aagttatcgc attcattatt agcaaaccaa
     2941 caaaaaaaaa ttcattaatg tgagaccacg aagtggctct tcagtggacg aaagggcctc
     3001 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3061 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3121 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3181 aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     3241 gcagtggcgg ttttcatggc ttgttatgac tgtttttttt t
//
