LOCUS       sequence-409600-        9589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express B4GALT3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Sakai E, Imaizumi T, Suzuki R, Taracena-Gandara M, Fujimoto T, 
            Sakurai F, Mizuguchi H
  TITLE     miR-27b targets MAIP1 to mediate lipid accumulation in cultured 
            human and mouse hepatic cells.
  JOURNAL   Commun Biol. 2023 Jun 24;6(1):669. doi: 10.1038/s42003-023-05049-w.
  PUBMED    37355744
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-06-24"; volume: "6(1):669. doi"; pages: " 
            10.1038/s42003-023-05049-w"
COMMENT     Sequence Label: CSII-TK-B4GALT3
FEATURES             Location/Qualifiers
     source          1..9589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2740..2884
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     misc_feature    4284..4872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4337..4357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4755..4766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5391..5571
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5597..5614)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5603..5827
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5873..6301
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5960..5979)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6170..6191
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6310..6330)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6315..6644
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6495..6630
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6557..6576
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6692..6739
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6758..7132
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7262..7383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7299..7318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7353..7372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7432..7448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7432..7448)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7445..7467)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7456..7472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7480..7486,7487..7504,7505..7510))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7525..7546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7663..7680)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7834..8422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7914..7933)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8593..9384,9385..9453))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9216..9235
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9454..9558)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtcc gtggcccgtt gctcgcgttt gctggcggtg
     2701 tccccggaag aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc
     2761 gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc
     2821 acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg
     2881 cttagcggcc gcggagtgct ggatggagcc tttctctgcc ctctgtgaca tttccaattt
     2941 tagataatgc ctcacatctc tgtccccccg ggaccccctg gagcccccat gatccctaag
     3001 aagacagctt gaacctagat ctcaccccca ggatgttgcg gaggctgctg gagcggcctt
     3061 gcacgctggc cctgcttgtg ggctcccagc tggctgtcat gatgtacctg tcactggggg
     3121 gcttccgaag tctcagtgcc ctatttggcc gagatcaggg accgacattt gactattctc
     3181 accctcgtga tgtctacagt aacctcagtc acctgcctgg ggccccaggg ggtcctccag
     3241 ctcctcaagg tctgccctac tgtccagaac gatctcctct cttagtgggt cctgtgtcgg
     3301 tgtcctttag cccagtgcca tcactggcag agattgtgga gcggaatccc cgggtagaac
     3361 cagggggccg gtaccgccct gcaggttgtg agccccgctc ccgaacagcc atcattgtgc
     3421 ctcatcgtgc ccgggagcac cacctgcgcc tgctgctcta ccacctgcac cccttcttgc
     3481 agcgccagca gcttgcttat ggcatctatg tcatccacca ggctggaaat ggaacattta
     3541 acagggcaaa actgttgaac gttggggtgc gagaggccct gcgtgatgaa gagtgggact
     3601 gcctgttctt gcacgatgtg gacctcttgc cagaaaatga ccacaatctg tatgtgtgtg
     3661 acccccgggg accccgccat gttgccgttg ctatgaacaa gtttggatac agcctcccgt
     3721 acccccagta cttcggagga gtctcagcac ttactcctga ccagtacctg aagatgaatg
     3781 gcttccccaa tgaatactgg ggctggggtg gtgaggatga cgacattgct accagggtgc
     3841 gcctggctgg gatgaagatc tctcggcccc ccacatctgt aggacactat aagatggtga
     3901 agcaccgagg agataagggc aatgaggaaa atccccacag atttgacctc ctggtccgta
     3961 cccagaattc ctggacgcaa gatgggatga actcactgac ataccagttg ctggctcgag
     4021 agctggggcc tctttatacc aacatcacag cagacattgg gactgaccct cggggtcctc
     4081 gggctccttc tgggccacgt tacccacctg gttcctccca agccttccgt caagagatgc
     4141 tgcaacgccg gcccccagcc aggcctgggc ctctatctac tgccaaccac acagccctcc
     4201 gaggttcaca ctgactcctc cttcctgtct accttaatca tgaaaccgtc tagagttaac
     4261 atcgagggat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4321 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4381 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4441 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4501 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4561 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4621 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     4681 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4741 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4801 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4861 ccgcctcccc gcatcgatac cgtcgagacc tagaaaaaca tggagcaatc acaagtagca
     4921 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     4981 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5041 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5101 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     5161 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     5221 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     5281 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     5341 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     5401 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5461 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5521 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     5581 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5641 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5701 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5761 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5821 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5881 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5941 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6001 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6061 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6121 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6181 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6241 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6301 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6361 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6421 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6481 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6541 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6601 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6661 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     6721 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     6781 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     6841 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     6901 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     6961 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     7021 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     7081 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     7141 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     7201 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     7261 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7381 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7441 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7501 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7561 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7621 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7681 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7741 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7801 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7861 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7921 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7981 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8041 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8101 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8161 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8221 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8281 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8341 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8401 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8461 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8521 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8581 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8641 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8701 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8761 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8821 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8881 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8941 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9001 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9061 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9121 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9181 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9241 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9301 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9361 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9421 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9481 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9541 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
