LOCUS       sequence-409608-        9148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express MAIP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Sakai E, Imaizumi T, Suzuki R, Taracena-Gandara M, Fujimoto T, 
            Sakurai F, Mizuguchi H
  TITLE     miR-27b targets MAIP1 to mediate lipid accumulation in cultured 
            human and mouse hepatic cells.
  JOURNAL   Commun Biol. 2023 Jun 24;6(1):669. doi: 10.1038/s42003-023-05049-w.
  PUBMED    37355744
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-06-24"; volume: "6(1):669. doi"; pages: " 
            10.1038/s42003-023-05049-w"
COMMENT     Sequence Label: CSII-TK-MAIP1
FEATURES             Location/Qualifiers
     source          1..9148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2740..2884
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     misc_feature    3843..4431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3896..3916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4314..4325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4950..5130
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5156..5173)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5162..5386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5432..5860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5519..5538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5729..5750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5869..5889)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5874..6203
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6054..6189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6116..6135
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6251..6298
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6317..6691
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6821..6942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6858..6877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6912..6931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6991..7007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6991..7007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7004..7026)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7015..7031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7039..7045,7046..7063,7064..7069))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7084..7105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7222..7239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7393..7981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7473..7492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8152..8943,8944..9012))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8775..8794
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9013..9117)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtcc gtggcccgtt gctcgcgttt gctggcggtg
     2701 tccccggaag aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc
     2761 gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc
     2821 acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg
     2881 cttagcggcc gcgggtcata aaaatggcgc tggccgctcg tttgctaccc cagttcctgc
     2941 actctcggtc gctgccctgc ggggccgtcc gactccggac tcctgctgtg gccgaggtga
     3001 ggctgccgtc ggccacactt tgctacttct gccgctgtcg cctcggcttg ggagcggcgt
     3061 tatttccacg aagcgctagg gccttggcag cctcggcgct acctgcccag ggctcccggt
     3121 ggccagtgct cagcagcccg ggactccccg cagccttcgc ttctttccct gcctgccctc
     3181 agcgcagcta cagcacggag gagaagcccc agcagcacca gaaaaccaag atgatcgtcc
     3241 tgggattctc caaccccatc aactgggtta ggactcgaat taaggccttc cttatctggg
     3301 cctatttcga caaagagttc agcatcacag agttctccga gggagcgaag caggcttttg
     3361 ctcatgtatc caagttgctg tcacagtgta aatttgatct gttggaagaa cttgtggcca
     3421 aagaggtgct acatgcattg aaagaaaagg ttacttcact acctgacaac cataaaaatg
     3481 cccttgctgc taacatagat gaaattgtat ttacatcaac aggagacatc tccatttact
     3541 atgatgagaa aggaaggaag tttgttaaca tcctgatgtg cttttggtat ctaaccagtg
     3601 ccaacatccc cagtgaaact ttaagaggag ccagtgtatt ccaggttaag ttggggaatc
     3661 agaatgtgga aactaaacaa cttcttagtg caagctatga gtttcagagg gagttcacac
     3721 aaggagtaaa gcctgactgg accattgcac ggattgaaca ctcaaaatta ttagaataat
     3781 tttcttggaa aaatcagctt atggacttct agagttaaca tcgagggatc aagcttatcg
     3841 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3901 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3961 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4021 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4081 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4141 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4201 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     4261 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4321 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4381 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     4441 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     4501 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     4561 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4621 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     4681 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     4741 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     4801 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     4861 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     4921 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     4981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5101 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     5161 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5221 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5281 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5341 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5401 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5461 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5521 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5581 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5641 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5701 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5761 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5821 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5881 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5941 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6001 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6061 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6121 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6181 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6241 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     6301 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     6361 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     6421 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     6481 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     6541 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     6601 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     6661 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     6721 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6781 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     6961 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7021 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7081 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7141 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7201 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7261 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7321 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7621 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7741 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7801 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7921 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7981 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8041 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8101 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8161 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8221 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8281 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8341 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8401 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8461 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8521 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8581 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8641 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8701 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8761 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8821 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8881 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8941 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9001 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9061 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9121 atttccccga aaagtgccac ctgacgtc
//
