LOCUS       sequence-409609-       10627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured 
            of BsaI-sites), Pm-->CBE:SpnCas9, PEM7-->non-specific sgRNA; 
            SmR/SpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Kozaeva E, Nielsen ZS, Nieto-Dominguez M, Nikel PI
  TITLE     The pAblo.pCasso self-curing vector toolset for unconstrained 
            cytidine and adenine base-editing in Gram-negative bacteria.
  JOURNAL   Nucleic Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236.
  PUBMED    38180826
REFERENCE   2  (bases 1 to 10627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236."
COMMENT     Sequence Label: pEditC
FEATURES             Location/Qualifiers
     source          1..10627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     191..210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(344..1177)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      1191..1542
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(1615..1701)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1726..2691)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        3591..3671
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             3731..4417
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(4466..4489,4490..4492,4493..8569)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     terminator      8642..8736
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        8759..8806
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_RNA        8864..8939
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             9190..9981
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            10127..10235
                     /note="incP origin of transfer"
     rep_origin      join(10329..10627,1..290)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
       61 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      121 ggagaaaggc ggacaggcat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      301 acgcggccgt gaaaggcagg ccggtccgtg gtggccacgg cctctaggcc agatccagcg
      361 gcatctgggt tagtcgagcg cgggccgctt cccatgtctc accagggcga gcctgtttcg
      421 cgatctcagc atctgaaatc ttcccggcct tgcgcttcgc tggggcctta cccaccgcct
      481 tggcgggctt cttcggtcca aaactgaaca acagatgtgt gaccttgcgc ccggtctttc
      541 gctgcgccca ctccacctgt agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa
      601 gcactcgcaa cttgaagtcc ttgatcgagg gataccggcc ttccagttga aaccactttc
      661 gcagctggtc aatttctatt tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt
      721 aaagcctgat cgcgtgggtg ctgtccatct tggccacgtc agccaaggcg tatttggtga
      781 actgtttggt gagttccgtc aggtacggca gcatgtcttt ggtgaacctg agttctacac
      841 ggccctcacc ctcccggtag atgattgttt gcacccagcc ggtaatcatc acactcggtc
      901 ttttcccctt gccattgggc tcttgggtta accggacttc ccgccgtttc aggcgcaggg
      961 ccgcttcttt gagctggttg taggaagatt cgatagggac acccgccatc gtcgctatgt
     1021 cctccgccgt cactgaatac atcacttcat cggtgacagg ctcgctcctc ttcacctggc
     1081 taatacaggc cagaacgatc cgctgttcct gaacactgag gcgatacgcg gcctcgacca
     1141 gggcattgct tttgtaaacc attgggggtg aggccacgtt cgacattcct tgtgtataag
     1201 gggacactgt atctgcgtcc cacaatacaa caaatccgtc cctttacaac aacaaatccg
     1261 tcccttctta acaacaaatc cgtcccttaa tggcaacaaa tccgtccctt tttaaactct
     1321 acaggccacg gattacgtgg cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga
     1381 aaagggtgga aacgcaaaaa acgcaccact acgtggcccc gttggggccg catttgtgcc
     1441 cctgaagggg cgggggaggc gtctgggcaa tccccgtttt accagtcccc tatcgccgcc
     1501 tgagagggcg caggaagcga gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa
     1561 ccagcggcac cagcggcgcc tgagaggggc gcgcccagct gtctagggcg gcggatttgt
     1621 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     1681 ctgagctttt cgttttattt gatgccttta attaaacgtt cgtaatcaag ccacttcctt
     1741 tttgcattga cgcagggtgt cggaaggcaa ctcgccgaac gcgctcctat agttttcagc
     1801 gaagcgtccc aaatgtaaga agccgtagtc tagggctatc tcagttatac tacgcacatt
     1861 ggcactggga tcgttcaagc aggcgcggat gctttcgagc ttgcggttgc ggatgtagtt
     1921 cttcggcgtg gtgccggcgt gcttctcgaa caaattgtag agcgagcgtg gactcatcat
     1981 cgccagctcc gctaaccgct caaggctgat attccgtttg agattctcct caatgaattg
     2041 aacgactcgc tcgaaagacg ggttaccttt gctgaaaatt tcacggctga cattgctgcc
     2101 cagcatttcg agcagcttgg aagcgatgat ccccgcatag tgctcttgga cccgaggcat
     2161 cgactttgta tgttccgctt cgtcacaaac taacccgagt agattgataa agccatcgag
     2221 ttgctggaga ttgtgtcgcg cggcgaaacg gataccctcc ctcggcttgt gccaattgtt
     2281 gtcactgcac gcccgatcaa ggaccactga gggcaattta acgataaatt tctcgcaatc
     2341 ttctgaatag gtcaggtcgg cttggtcatc cggattgagc agcaatagtt cgcccggcgc
     2401 aaaatagtgc tcctggccat ggccacgcca caggcaatgg cctttgagta ttatttgcag
     2461 atgataacag gtttctaatc caggcgagat taccctcacg ctaccgccgt agctgattcg
     2521 acacagatcg aggcatccga agattctgtg gtgcagcctg cctgccgggc gcccgccctt
     2581 gggcaggcga atagagtgcg taccgacata ctggttaaca taatcggaga ctgcataggg
     2641 ctcggcgtgg acgaagatct gacttttctc gttcaataag caaaaatcca tagttcacgg
     2701 ttctcttatt ttaatgtggg ctgcttggtg tgatgtagaa aggcgccaag tcgatgaaaa
     2761 tgcatctcga cgtgatgcgt atacgggtta cccccattgc cacgttgcgc catccttttt
     2821 gcaatcagtg accacttttc caagcaaaaa taacgccaag cagaacgaag acgttctttt
     2881 taagaagcga gaacaccaga agttcgtgct gtcggggcat ggggcgacga attggcggat
     2941 aaaggggatc tgctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     3001 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc gtaattacgt
     3061 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     3121 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     3181 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     3241 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     3301 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgcat
     3361 gggaattagc ttgatctgac caacgaccgg tagcggagct atccaacggc ggtataccag
     3421 gaaaacacac agcaggtaca tcagaacagt accatgactg aagaacaaat agttttttcc
     3481 tgatccataa agcagaacgg cctgctccat gacaaatctg gctccccaac taatgcccca
     3541 tgcagccagc ataaccagca taaagtgcag tgtccggttt gatagggata agtccagcct
     3601 tgcaagaagc ggatacagga gtgcaaaaaa tggctatctc tagtaaggcc taccccttag
     3661 gctttatgca acagaaacaa taataatgga gtcatgacca tgcctaggcc gcggttagga
     3721 ggaaaaacat atgagtagcg aaaccggtcc ggttgcagtg gatccgaccc tgcgccgtcg
     3781 cattgaaccg cacgagtttg aagtgttctt tgatccgcgc gagctgcgca aagaaacttg
     3841 cctgctgtac gagattaact ggggtggccg ccatagcatc tggcgccata ccagccagaa
     3901 caccaataag cacgtggaag tgaattttat tgaaaaattt accaccgagc gctacttctg
     3961 ccctaatacc cgctgcagca tcacctggtt tctgagctgg agcccgtgcg gcgaatgtag
     4021 tcgcgccatt accgagttcc tgagccgcta tccgcatgtg accctgttca tctacatcgc
     4081 ccgtctgtac catcacgccg atccgcgcaa tcgccaaggt ctgcgtgatc tgattagcag
     4141 cggtgtgacc atccagatca tgaccgaaca agagagcggc tattgctggc gcaacttcgt
     4201 gaactattct ccgagcaacg aagcccactg gccgcgttat ccgcatctgt gggtgcgcct
     4261 gtatgtgctg gagctgtact gcatcatcct gggcctgccg ccttgcctga atattctgcg
     4321 ccgtaaacag ccgcaactga cattcttcac catcgcactg cagagctgcc attatcagcg
     4381 cctgccgccg cacattttat gggccaccgg tctgaaaagc ggtagtgaaa ctccgggcac
     4441 aagcgaaagc gcaaccccgg aaagtgataa gaaatactca ataggcttag ctatcggcac
     4501 aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa
     4561 ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt
     4621 tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac
     4681 acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt
     4741 agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca
     4801 tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc
     4861 aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt
     4921 aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga
     4981 tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa
     5041 tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc
     5101 tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa
     5161 gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa
     5221 atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga
     5281 tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc
     5341 taagaattta tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac
     5401 taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac
     5461 tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga
     5521 tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag aagaatttta
     5581 taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact
     5641 aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca
     5701 aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt
     5761 aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg
     5821 tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat
     5881 taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga
     5941 acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt
     6001 gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg
     6061 aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt
     6121 caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga
     6181 atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac
     6241 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga
     6301 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga
     6361 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg
     6421 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa
     6481 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt
     6541 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt
     6601 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat
     6661 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg
     6721 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg
     6781 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag
     6841 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct
     6901 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt
     6961 aagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg attcaataga
     7021 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga
     7081 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac
     7141 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa
     7201 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca
     7261 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt
     7321 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta
     7381 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt
     7441 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta
     7501 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac
     7561 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc
     7621 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt
     7681 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa
     7741 tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa
     7801 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg
     7861 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa
     7921 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc
     7981 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga
     8041 cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat
     8101 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt
     8161 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga
     8221 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat
     8281 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc
     8341 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt
     8401 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg
     8461 taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac
     8521 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactgat ctagagtcga
     8581 cctgcaggca tgcaagcttg cggccgcgtc gtgactggga aaaccctggc gactagtctt
     8641 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg
     8701 ctcggttgcc gccgggcgtt ttttattggt gagaatccag gggtcccttt aattaattgt
     8761 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     8821 aacccctagg ccgcggccgc gcggagaccc gagactggtc tcagttttag agctagaaat
     8881 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgca
     8941 attcgagctc ggtacccggg gatcccaata attacgattt acgtatttaa atgaaccttg
     9001 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     9061 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     9121 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     9181 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     9241 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     9301 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     9361 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     9421 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     9481 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     9541 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     9601 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     9661 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     9721 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     9781 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     9841 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     9901 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     9961 accaaggtag tcggcaaata agacaattgt ccttttccgc tgcataaccc tgcttcgggg
    10021 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc
    10081 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg
    10141 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
    10201 tgctcaacgg gaatcctgct ctgcgaggct ggccgtaggc cggccgataa tctcatgacc
    10261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10441 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10621 ccggata
//
