LOCUS       sequence-409612-       10489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured 
            of BsaI-sites), Pm-->ABE:SpRY, PEM7-->non-specific sgRNA; SmR/SpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Kozaeva E, Nielsen ZS, Nieto-Dominguez M, Nikel PI
  TITLE     The pAblo.pCasso self-curing vector toolset for unconstrained 
            cytidine and adenine base-editing in Gram-negative bacteria.
  JOURNAL   Nucleic Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236.
  PUBMED    38180826
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236."
COMMENT     Sequence Label: pEditA-RY
FEATURES             Location/Qualifiers
     source          1..10489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     191..210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(344..1177)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      1191..1542
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(1615..1701)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1726..2691)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        3591..3671
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             4328..6043
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     terminator      8504..8598
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        8621..8668
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_RNA        8726..8801
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             9052..9843
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            9989..10097
                     /note="incP origin of transfer"
     rep_origin      join(10191..10489,1..290)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
       61 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      121 ggagaaaggc ggacaggcat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      301 acgcggccgt gaaaggcagg ccggtccgtg gtggccacgg cctctaggcc agatccagcg
      361 gcatctgggt tagtcgagcg cgggccgctt cccatgtctc accagggcga gcctgtttcg
      421 cgatctcagc atctgaaatc ttcccggcct tgcgcttcgc tggggcctta cccaccgcct
      481 tggcgggctt cttcggtcca aaactgaaca acagatgtgt gaccttgcgc ccggtctttc
      541 gctgcgccca ctccacctgt agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa
      601 gcactcgcaa cttgaagtcc ttgatcgagg gataccggcc ttccagttga aaccactttc
      661 gcagctggtc aatttctatt tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt
      721 aaagcctgat cgcgtgggtg ctgtccatct tggccacgtc agccaaggcg tatttggtga
      781 actgtttggt gagttccgtc aggtacggca gcatgtcttt ggtgaacctg agttctacac
      841 ggccctcacc ctcccggtag atgattgttt gcacccagcc ggtaatcatc acactcggtc
      901 ttttcccctt gccattgggc tcttgggtta accggacttc ccgccgtttc aggcgcaggg
      961 ccgcttcttt gagctggttg taggaagatt cgatagggac acccgccatc gtcgctatgt
     1021 cctccgccgt cactgaatac atcacttcat cggtgacagg ctcgctcctc ttcacctggc
     1081 taatacaggc cagaacgatc cgctgttcct gaacactgag gcgatacgcg gcctcgacca
     1141 gggcattgct tttgtaaacc attgggggtg aggccacgtt cgacattcct tgtgtataag
     1201 gggacactgt atctgcgtcc cacaatacaa caaatccgtc cctttacaac aacaaatccg
     1261 tcccttctta acaacaaatc cgtcccttaa tggcaacaaa tccgtccctt tttaaactct
     1321 acaggccacg gattacgtgg cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga
     1381 aaagggtgga aacgcaaaaa acgcaccact acgtggcccc gttggggccg catttgtgcc
     1441 cctgaagggg cgggggaggc gtctgggcaa tccccgtttt accagtcccc tatcgccgcc
     1501 tgagagggcg caggaagcga gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa
     1561 ccagcggcac cagcggcgcc tgagaggggc gcgcccagct gtctagggcg gcggatttgt
     1621 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     1681 ctgagctttt cgttttattt gatgccttta attaaacgtt cgtaatcaag ccacttcctt
     1741 tttgcattga cgcagggtgt cggaaggcaa ctcgccgaac gcgctcctat agttttcagc
     1801 gaagcgtccc aaatgtaaga agccgtagtc tagggctatc tcagttatac tacgcacatt
     1861 ggcactggga tcgttcaagc aggcgcggat gctttcgagc ttgcggttgc ggatgtagtt
     1921 cttcggcgtg gtgccggcgt gcttctcgaa caaattgtag agcgagcgtg gactcatcat
     1981 cgccagctcc gctaaccgct caaggctgat attccgtttg agattctcct caatgaattg
     2041 aacgactcgc tcgaaagacg ggttaccttt gctgaaaatt tcacggctga cattgctgcc
     2101 cagcatttcg agcagcttgg aagcgatgat ccccgcatag tgctcttgga cccgaggcat
     2161 cgactttgta tgttccgctt cgtcacaaac taacccgagt agattgataa agccatcgag
     2221 ttgctggaga ttgtgtcgcg cggcgaaacg gataccctcc ctcggcttgt gccaattgtt
     2281 gtcactgcac gcccgatcaa ggaccactga gggcaattta acgataaatt tctcgcaatc
     2341 ttctgaatag gtcaggtcgg cttggtcatc cggattgagc agcaatagtt cgcccggcgc
     2401 aaaatagtgc tcctggccat ggccacgcca caggcaatgg cctttgagta ttatttgcag
     2461 atgataacag gtttctaatc caggcgagat taccctcacg ctaccgccgt agctgattcg
     2521 acacagatcg aggcatccga agattctgtg gtgcagcctg cctgccgggc gcccgccctt
     2581 gggcaggcga atagagtgcg taccgacata ctggttaaca taatcggaga ctgcataggg
     2641 ctcggcgtgg acgaagatct gacttttctc gttcaataag caaaaatcca tagttcacgg
     2701 ttctcttatt ttaatgtggg ctgcttggtg tgatgtagaa aggcgccaag tcgatgaaaa
     2761 tgcatctcga cgtgatgcgt atacgggtta cccccattgc cacgttgcgc catccttttt
     2821 gcaatcagtg accacttttc caagcaaaaa taacgccaag cagaacgaag acgttctttt
     2881 taagaagcga gaacaccaga agttcgtgct gtcggggcat ggggcgacga attggcggat
     2941 aaaggggatc tgctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     3001 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc gtaattacgt
     3061 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     3121 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     3181 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     3241 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     3301 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgcat
     3361 gggaattagc ttgatctgac caacgaccgg tagcggagct atccaacggc ggtataccag
     3421 gaaaacacac agcaggtaca tcagaacagt accatgactg aagaacaaat agttttttcc
     3481 tgatccataa agcagaacgg cctgctccat gacaaatctg gctccccaac taatgcccca
     3541 tgcagccagc ataaccagca taaagtgcag tgtccggttt gatagggata agtccagcct
     3601 tgcaagaagc ggatacagga gtgcaaaaaa tggctatctc tagtaaggcc taccccttag
     3661 gctttatgca acagaaacaa taataatgga gtcatgacca tgcctaggcc gcggttagga
     3721 ggaaaaacat atgagcgaag tcgagttctc ccatgagtat tggatgcggc acgctctgac
     3781 cctcgcgaaa cgtgctcgcg acgagcggga agtcccagtg ggcgctgtcc tcgtgctgaa
     3841 taaccgcgtg attggcgaag gctggaatcg ggccatcgga ctccatgatc ctacggcaca
     3901 cgcagaaatc atggccctgc gccagggtgg cctcgtcatg caaaattatc ggctgtacga
     3961 cgccacgttg tattccacct tcgagccctg cgtcatgtgt gccggggcaa tgattcactc
     4021 ccgtattggg cgtgtggtgt tcggggtgcg caatgcaaag accggcgctg ccggctcgct
     4081 catggatgtc ttgcaccatc ccggtatgaa ccaccgggtc gagattaccg aaggcatttt
     4141 ggccgacgag tgcgccgcgc tcttgtgccg gttttttcgt atgccacggc gtgtgttcaa
     4201 cgctcaaaag aaagcacaaa gctccaccga ttccgggggt tcctcgggcg gctcgagcgg
     4261 gtccgaaacc cctggtacgt cggaatcggc tacgccagaa tcgtccggcg gctcgtccgg
     4321 cggttctgac aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc
     4381 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     4441 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     4501 cgagagaacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     4561 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     4621 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     4681 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     4741 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     4801 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     4861 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     4921 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     4981 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     5041 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     5101 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     5161 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     5221 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     5281 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     5341 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     5401 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     5461 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     5521 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     5581 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     5641 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     5701 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     5761 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     5821 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     5881 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcttt
     5941 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     6001 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgttttg atagtgttga
     6061 aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa
     6121 aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat
     6181 tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata
     6241 tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg
     6301 gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat
     6361 attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga
     6421 tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag
     6481 tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca
     6541 gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat
     6601 cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga
     6661 gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca
     6721 tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg
     6781 aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga
     6841 tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg
     6901 ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat
     6961 gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa
     7021 tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg
     7081 ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat
     7141 gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa
     7201 atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa
     7261 caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa
     7321 gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg
     7381 taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta
     7441 ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa
     7501 acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga
     7561 ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga
     7621 agtacagaca ggcggattct ccaaggagtc aatccggccc aagcggaaca gcgataagct
     7681 catcgcgcgg aaaaaagatt gggaccctaa aaagtatggg gggttcttgt ggccgacggt
     7741 cgcttacagc gtcttggtcg tggcaaaggt cgaaaagggc aagtcgaaaa agctcaaatc
     7801 ggtcaaagag ttgttgggta ttacgattat ggagcggtcc tcgttcgaaa agaaccccat
     7861 cgactttctg gaagccaaag gctataagga agtcaagaag gatctcatca tcaaattgcc
     7921 gaaatattcc ctctttgaat tggaaaacgg tcgtaagcgt atgctggcct cggcgaagca
     7981 actgcagaaa gggaacgaat tggcgctccc ttccaagtat gtgaacttcc tctacctcgc
     8041 gtcgcactac gagaagttga aagggtcgcc agaagataat gaacaaaagc agctctttgt
     8101 ggagcagcac aagcattacc tcgacgagat tatcgagcaa attagcgagt tcagcaaacg
     8161 ggtcattctc gccgacgcca atctcgacaa agtgctgtcc gcatacaata agcatcggga
     8221 caaaccaatt cgggagcaag ccgaaaatat catccacctg tttaccttga cccgcctggg
     8281 tgcccctcgc gcgttcaagt actttgatac caccatcgac ccaaaacagt accggtccac
     8341 gaaggaggtc ctcgatgcta ccctcatcca tcagagcatt accggcctct atgagacgcg
     8401 catcgacctc tcgcagctgg ggggtgattg atctagagtc gacctgcagg catgcaagct
     8461 tgcggccgcg tcgtgactgg gaaaaccctg gcgactagtc ttggactcct gttgatagat
     8521 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     8581 ttttttattg gtgagaatcc aggggtccct ttaattaatt gttgacaatt aatcatcggc
     8641 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccccta ggccgcggcc
     8701 gcgcggagac ccgagactgg tctcagtttt agagctagaa atagcaagtt aaaataaggc
     8761 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg caattcgagc tcggtacccg
     8821 gggatcccaa taattacgat ttacgtattt aaatgaacct tgaccgaacg cagcggtggt
     8881 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     8941 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     9001 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     9061 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     9121 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     9181 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     9241 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     9301 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     9361 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     9421 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     9481 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     9541 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     9601 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     9661 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     9721 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     9781 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     9841 taagacaatt gtccttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
     9901 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
     9961 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
    10021 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
    10081 ctctgcgagg ctggccgtag gccggccgat aatctcatga ccaaaatccc ttaacgtgag
    10141 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10201 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10261 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10321 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10381 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10441 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggata
//
