LOCUS       sequence-409613-       11158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured 
            of BsaI-sites), Pm-->CBE:SpRY, PEM7-->non-specific sgRNA; SmR/SpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Kozaeva E, Nielsen ZS, Nieto-Dominguez M, Nikel PI
  TITLE     The pAblo.pCasso self-curing vector toolset for unconstrained 
            cytidine and adenine base-editing in Gram-negative bacteria.
  JOURNAL   Nucleic Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236.
  PUBMED    38180826
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236."
COMMENT     Sequence Label: pEditC-RY
FEATURES             Location/Qualifiers
     source          1..11158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     191..210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(344..1177)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      1191..1542
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(1615..1701)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1726..2691)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        3591..3671
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             3731..4417
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             4466..6181
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             8597..8845
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8876..9124
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKM"
     terminator      9173..9267
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        9290..9337
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_RNA        9395..9470
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             9721..10512
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            10658..10766
                     /note="incP origin of transfer"
     rep_origin      join(10860..11158,1..290)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
       61 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      121 ggagaaaggc ggacaggcat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      301 acgcggccgt gaaaggcagg ccggtccgtg gtggccacgg cctctaggcc agatccagcg
      361 gcatctgggt tagtcgagcg cgggccgctt cccatgtctc accagggcga gcctgtttcg
      421 cgatctcagc atctgaaatc ttcccggcct tgcgcttcgc tggggcctta cccaccgcct
      481 tggcgggctt cttcggtcca aaactgaaca acagatgtgt gaccttgcgc ccggtctttc
      541 gctgcgccca ctccacctgt agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa
      601 gcactcgcaa cttgaagtcc ttgatcgagg gataccggcc ttccagttga aaccactttc
      661 gcagctggtc aatttctatt tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt
      721 aaagcctgat cgcgtgggtg ctgtccatct tggccacgtc agccaaggcg tatttggtga
      781 actgtttggt gagttccgtc aggtacggca gcatgtcttt ggtgaacctg agttctacac
      841 ggccctcacc ctcccggtag atgattgttt gcacccagcc ggtaatcatc acactcggtc
      901 ttttcccctt gccattgggc tcttgggtta accggacttc ccgccgtttc aggcgcaggg
      961 ccgcttcttt gagctggttg taggaagatt cgatagggac acccgccatc gtcgctatgt
     1021 cctccgccgt cactgaatac atcacttcat cggtgacagg ctcgctcctc ttcacctggc
     1081 taatacaggc cagaacgatc cgctgttcct gaacactgag gcgatacgcg gcctcgacca
     1141 gggcattgct tttgtaaacc attgggggtg aggccacgtt cgacattcct tgtgtataag
     1201 gggacactgt atctgcgtcc cacaatacaa caaatccgtc cctttacaac aacaaatccg
     1261 tcccttctta acaacaaatc cgtcccttaa tggcaacaaa tccgtccctt tttaaactct
     1321 acaggccacg gattacgtgg cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga
     1381 aaagggtgga aacgcaaaaa acgcaccact acgtggcccc gttggggccg catttgtgcc
     1441 cctgaagggg cgggggaggc gtctgggcaa tccccgtttt accagtcccc tatcgccgcc
     1501 tgagagggcg caggaagcga gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa
     1561 ccagcggcac cagcggcgcc tgagaggggc gcgcccagct gtctagggcg gcggatttgt
     1621 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     1681 ctgagctttt cgttttattt gatgccttta attaaacgtt cgtaatcaag ccacttcctt
     1741 tttgcattga cgcagggtgt cggaaggcaa ctcgccgaac gcgctcctat agttttcagc
     1801 gaagcgtccc aaatgtaaga agccgtagtc tagggctatc tcagttatac tacgcacatt
     1861 ggcactggga tcgttcaagc aggcgcggat gctttcgagc ttgcggttgc ggatgtagtt
     1921 cttcggcgtg gtgccggcgt gcttctcgaa caaattgtag agcgagcgtg gactcatcat
     1981 cgccagctcc gctaaccgct caaggctgat attccgtttg agattctcct caatgaattg
     2041 aacgactcgc tcgaaagacg ggttaccttt gctgaaaatt tcacggctga cattgctgcc
     2101 cagcatttcg agcagcttgg aagcgatgat ccccgcatag tgctcttgga cccgaggcat
     2161 cgactttgta tgttccgctt cgtcacaaac taacccgagt agattgataa agccatcgag
     2221 ttgctggaga ttgtgtcgcg cggcgaaacg gataccctcc ctcggcttgt gccaattgtt
     2281 gtcactgcac gcccgatcaa ggaccactga gggcaattta acgataaatt tctcgcaatc
     2341 ttctgaatag gtcaggtcgg cttggtcatc cggattgagc agcaatagtt cgcccggcgc
     2401 aaaatagtgc tcctggccat ggccacgcca caggcaatgg cctttgagta ttatttgcag
     2461 atgataacag gtttctaatc caggcgagat taccctcacg ctaccgccgt agctgattcg
     2521 acacagatcg aggcatccga agattctgtg gtgcagcctg cctgccgggc gcccgccctt
     2581 gggcaggcga atagagtgcg taccgacata ctggttaaca taatcggaga ctgcataggg
     2641 ctcggcgtgg acgaagatct gacttttctc gttcaataag caaaaatcca tagttcacgg
     2701 ttctcttatt ttaatgtggg ctgcttggtg tgatgtagaa aggcgccaag tcgatgaaaa
     2761 tgcatctcga cgtgatgcgt atacgggtta cccccattgc cacgttgcgc catccttttt
     2821 gcaatcagtg accacttttc caagcaaaaa taacgccaag cagaacgaag acgttctttt
     2881 taagaagcga gaacaccaga agttcgtgct gtcggggcat ggggcgacga attggcggat
     2941 aaaggggatc tgctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     3001 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc gtaattacgt
     3061 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     3121 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     3181 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     3241 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     3301 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgcat
     3361 gggaattagc ttgatctgac caacgaccgg tagcggagct atccaacggc ggtataccag
     3421 gaaaacacac agcaggtaca tcagaacagt accatgactg aagaacaaat agttttttcc
     3481 tgatccataa agcagaacgg cctgctccat gacaaatctg gctccccaac taatgcccca
     3541 tgcagccagc ataaccagca taaagtgcag tgtccggttt gatagggata agtccagcct
     3601 tgcaagaagc ggatacagga gtgcaaaaaa tggctatctc tagtaaggcc taccccttag
     3661 gctttatgca acagaaacaa taataatgga gtcatgacca tgcctaggcc gcggttagga
     3721 ggaaaaacat atgagtagcg aaaccggtcc ggttgcagtg gatccgaccc tgcgccgtcg
     3781 cattgaaccg cacgagtttg aagtgttctt tgatccgcgc gagctgcgca aagaaacttg
     3841 cctgctgtac gagattaact ggggtggccg ccatagcatc tggcgccata ccagccagaa
     3901 caccaataag cacgtggaag tgaattttat tgaaaaattt accaccgagc gctacttctg
     3961 ccctaatacc cgctgcagca tcacctggtt tctgagctgg agcccgtgcg gcgaatgtag
     4021 tcgcgccatt accgagttcc tgagccgcta tccgcatgtg accctgttca tctacatcgc
     4081 ccgtctgtac catcacgccg atccgcgcaa tcgccaaggt ctgcgtgatc tgattagcag
     4141 cggtgtgacc atccagatca tgaccgaaca agagagcggc tattgctggc gcaacttcgt
     4201 gaactattct ccgagcaacg aagcccactg gccgcgttat ccgcatctgt gggtgcgcct
     4261 gtatgtgctg gagctgtact gcatcatcct gggcctgccg ccttgcctga atattctgcg
     4321 ccgtaaacag ccgcaactga cattcttcac catcgcactg cagagctgcc attatcagcg
     4381 cctgccgccg cacattttat gggccaccgg tctgaaaagc ggtagtgaaa ctccgggcac
     4441 aagcgaaagc gcaaccccgg aaagtgacaa gaagtacagc atcggcctgg ccatcggcac
     4501 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     4561 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     4621 cgacagcggc gaaacagccg agagaacccg gctgaagaga accgccagaa gaagatacac
     4681 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     4741 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     4801 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     4861 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggctg acctgcggct
     4921 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     4981 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     5041 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     5101 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     5161 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     5221 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     5281 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     5341 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     5401 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     5461 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     5521 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     5581 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     5641 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     5701 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     5761 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     5821 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     5881 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     5941 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     6001 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     6061 aatgagaaag cccgctttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     6121 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     6181 gtgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac
     6241 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga
     6301 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga
     6361 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg
     6421 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa
     6481 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt
     6541 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt
     6601 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat
     6661 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg
     6721 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg
     6781 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag
     6841 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct
     6901 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt
     6961 aagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg attcaataga
     7021 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga
     7081 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac
     7141 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa
     7201 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca
     7261 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt
     7321 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta
     7381 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt
     7441 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta
     7501 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac
     7561 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc
     7621 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt
     7681 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa
     7741 tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa tccggcccaa
     7801 gcggaacagc gataagctca tcgcgcggaa aaaagattgg gaccctaaaa agtatggggg
     7861 gttcttgtgg ccgacggtcg cttacagcgt cttggtcgtg gcaaaggtcg aaaagggcaa
     7921 gtcgaaaaag ctcaaatcgg tcaaagagtt gttgggtatt acgattatgg agcggtcctc
     7981 gttcgaaaag aaccccatcg actttctgga agccaaaggc tataaggaag tcaagaagga
     8041 tctcatcatc aaattgccga aatattccct ctttgaattg gaaaacggtc gtaagcgtat
     8101 gctggcctcg gcgaagcaac tgcagaaagg gaacgaattg gcgctccctt ccaagtatgt
     8161 gaacttcctc tacctcgcgt cgcactacga gaagttgaaa gggtcgccag aagataatga
     8221 acaaaagcag ctctttgtgg agcagcacaa gcattacctc gacgagatta tcgagcaaat
     8281 tagcgagttc agcaaacggg tcattctcgc cgacgccaat ctcgacaaag tgctgtccgc
     8341 atacaataag catcgggaca aaccaattcg ggagcaagcc gaaaatatca tccacctgtt
     8401 taccttgacc cgcctgggtg cccctcgcgc gttcaagtac tttgatacca ccatcgaccc
     8461 aaaacagtac cggtccacga aggaggtcct cgatgctacc ctcatccatc agagcattac
     8521 cggcctctat gagacgcgca tcgacctctc gcagctgggg ggtgatagcg gcgggagcgg
     8581 cgggagcggg gggagcacta acctcagcga cattatcgag aaggaaaccg ggaagcaact
     8641 ggtgattcag gagagcatcc tgatgctccc cgaggaggtg gaggaagtga tcgggaacaa
     8701 gccggagagc gacatcttgg tccacaccgc ctatgacgaa agcaccgatg agaacgtcat
     8761 gctcttgacg tccgatgcgc ctgagtacaa gccgtgggcc ctcgtcattc aagactccaa
     8821 tggtgaaaac aaaattaaga tgctgagcgg aggatccgga ggatctggag gcagcactaa
     8881 cctcagcgac attatcgaga aggaaaccgg gaagcaactg gtgattcagg agagcatcct
     8941 gatgctcccc gaggaggtgg aggaagtgat cgggaacaag ccggagagcg acatcttggt
     9001 ccacaccgcc tatgacgaaa gcaccgatga gaacgtcatg ctcttgacgt ccgatgcgcc
     9061 tgagtacaag ccgtgggccc tcgtcattca agactccaat ggtgaaaaca aaattaagat
     9121 gtgaaagctt gcggccgcgt cgtgactggg aaaaccctgg cgactagtct tggactcctg
     9181 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
     9241 cgccgggcgt tttttattgg tgagaatcca ggggtccctt taattaattg ttgacaatta
     9301 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccctag
     9361 gccgcggccg cgcggagacc cgagactggt ctcagtttta gagctagaaa tagcaagtta
     9421 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc aattcgagct
     9481 cggtacccgg ggatcccaat aattacgatt tacgtattta aatgaacctt gaccgaacgc
     9541 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     9601 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     9661 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     9721 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     9781 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     9841 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     9901 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     9961 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
    10021 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
    10081 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
    10141 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
    10201 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
    10261 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
    10321 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
    10381 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
    10441 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
    10501 gtcggcaaat aagacaattg tccttttccg ctgcataacc ctgcttcggg gtcattatag
    10561 cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc
    10621 gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc
    10681 cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg
    10741 ggaatcctgc tctgcgaggc tggccgtagg ccggccgata atctcatgac caaaatccct
    10801 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10861 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10921 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10981 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    11041 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    11101 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggata
//
