LOCUS       sequence-409623-        8500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac-based transposon vector plasmid which encodes an expression
            unit of tetracycline-responsive transactivator under control of HSP 
            promoter with transcriptional amplification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Kitano H, Nagae Y, Kawabe Y, Ito A, Kamihira M
  TITLE     Development of a genetically modified hepatoma cell line with 
            heat-inducible high liver function.
  JOURNAL   Cytotechnology. 2021 Jun;73(3):353-362. doi: 
            10.1007/s10616-021-00457-4. Epub 2021 Feb 24.
  PUBMED    34149171
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10616-021-00457-4"; journalName: "Cytotechnology"; date: 
            "2021-06"; volume: "73"; issue: "3"; pages: "353-362"
COMMENT     Sequence Label: PB/pHSP-tTA-TRE-tTA-pEF1-alpha-PuroR
FEATURES             Location/Qualifiers
     source          1..8500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2611..2924
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2655..2673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2690..2708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2726..2744
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2762..2780
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2797..2815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2833..2851
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2898..2922
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3763..3974
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3987..4255
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4286..4295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4298..4309
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4301..4354
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4355..4954
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4355..4374)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4851..4871
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5008..5028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5070..5191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5107..5126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5161..5180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5954..5988
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6479..6495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6479..6495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6492..6514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6503..6519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6527..6533,6534..6551,6552..6557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6572..6593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6710..6727)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6881..7469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6961..6980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7640..8431,8432..8500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8263..8282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtgcctctaa agttgctgct
     1381 tttgcagcct ctgccacaac cgcgcgtcct cagagccagc ccggaggagc tagaaccttc
     1441 cccgcatttc tttcagcagc ctgagtcaga ggcgggctgg cctggcgtag ccgcccagcc
     1501 tcgcggctca tgccccgatc tgcccgaacc ttctcccggg gtcagcgccg cgccgcgcca
     1561 cccggctgag tcagcccggg cgggcgagag gctctcaact gggcgggaag gtgcgggaag
     1621 gtgcggaaag gttcgcgaaa gttcgcggcg gcgggggtcg ggtgaggcgc aaaaggataa
     1681 aaagccggtg gaagcggagc tgagcagatc cgagccgggc tggctgcaga gaaaccgcag
     1741 ggagagcctc actgctgagc gcccctcgac ggcggagcgg cagcagcctc cgtggcctcc
     1801 agcatccgac aagtctagag ccgccaccat gagcaggctg gacaagagca aggtgatcaa
     1861 cagcgccctg gagctgctga acgaggtggg catcgagggc ctgaccacaa gaaagctggc
     1921 ccagaagctg ggagtggagc agcccaccct gtactggcac gtgaagaaca agagggccct
     1981 cctggatgcc ctggccatcg agatgctgga cagacaccac acacactttt gccccctgga
     2041 gggagagagc tggcaggact tcctgaggaa caacgccaag agcttcaggt gtgccctgct
     2101 gagccacagg gacggagcca aggtgcacct gggcaccagg cctaccgaga agcagtacga
     2161 gaccctggag aaccaactgg ccttcctgtg ccagcagggc ttcagcctgg agaatgctct
     2221 gtacgccctg tccgctgtgg gccacttcac cctgggctgt gtgctggagg atcaggagca
     2281 tcaggtggcc aaagaggaga gggagacccc cacaaccgac agcatgcctc ctctgctgag
     2341 gcaggctatt gagctgttcg accaccaggg cgctgaaccc gccttcctgt tcggcctgga
     2401 gctgatcatc tgtggcctgg agaagcagct gaagtgcgag tccggaggac ccgctgatgc
     2461 cctggatgat ttcgatctgg atatgctgcc cgccgacgcc ctggacgact ttgacctgga
     2521 catgctccct gccgacgccc tggatgactt cgacctggac atgctgcccg gatgatctag
     2581 acaggtaagt aggccctttc gtcttcactc gagtttactc cctatcagtg atagagaacg
     2641 tatgtcgagt ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt
     2701 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     2761 ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta
     2821 tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg
     2881 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcatcgat actgacatcc
     2941 actttgcctt tctctccaca gggaattcgc cgccaccatg agcaggctgg acaagagcaa
     3001 ggtgatcaac agcgccctgg agctgctgaa cgaggtgggc atcgagggcc tgaccacaag
     3061 aaagctggcc cagaagctgg gagtggagca gcccaccctg tactggcacg tgaagaacaa
     3121 gagggccctc ctggatgccc tggccatcga gatgctggac agacaccaca cacacttttg
     3181 ccccctggag ggagagagct ggcaggactt cctgaggaac aacgccaaga gcttcaggtg
     3241 tgccctgctg agccacaggg acggagccaa ggtgcacctg ggcaccaggc ctaccgagaa
     3301 gcagtacgag accctggaga accaactggc cttcctgtgc cagcagggct tcagcctgga
     3361 gaatgctctg tacgccctgt ccgctgtggg ccacttcacc ctgggctgtg tgctggagga
     3421 tcaggagcat caggtggcca aagaggagag ggagaccccc acaaccgaca gcatgcctcc
     3481 tctgctgagg caggctattg agctgttcga ccaccagggc gctgaacccg ccttcctgtt
     3541 cggcctggag ctgatcatct gtggcctgga gaagcagctg aagtgcgagt ccggaggacc
     3601 cgctgatgcc ctggatgatt tcgatctgga tatgctgccc gccgacgccc tggacgactt
     3661 tgacctggac atgctccctg ccgacgccct ggatgacttc gacctggaca tgctgcccgg
     3721 atgagctagc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     3781 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     3841 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3901 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3961 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     4021 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     4081 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     4141 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     4201 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     4261 tgtgaccggc gcctacggat ccgccgccac catggggatc gagggcagag gaagtcttct
     4321 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     4381 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4441 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4501 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4561 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4621 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4681 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4741 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4801 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4861 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4921 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     4981 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5041 tttaatgcct ttgtatcatg cgttaactaa acttgtttat tgcagcttat aatggttaca
     5101 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5161 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gaattgactc aaatgatgtc
     5221 aattagtcta tcagaagctc atctggtctc ccttccgggg gacaagacat ccctgtttaa
     5281 tatttaaaca gcagtgttcc caaactgggt tcttatatcc cttgctctgg tcaaccaggt
     5341 tgcagggttt cctgtcctca caggaacgaa gtccctaaag aaacagtggc agccaggttt
     5401 agccccggaa ttgactggat tcctttttta gggcccattg gtatggcttt ttccccgtat
     5461 ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg
     5521 tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga
     5581 gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg
     5641 taattacatc cctgggggct ttgggggggg gctgtccctg atatctataa caagaaaata
     5701 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
     5761 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
     5821 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
     5881 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     5941 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg
     6001 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
     6061 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     6121 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     6181 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     6241 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     6301 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     6361 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     6421 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt
     6481 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6541 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6601 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6661 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7321 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7921 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8221 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat
//
