LOCUS       sequence-409736-       11894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for expression of HLA-E SCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: TRE-UBC-HLA-E SCT-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..11894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(257..361)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(436..455)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        627..1006
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1008..1206
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1224..1404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1451..1576
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2069..2302
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2487..2531
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2829..2946
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2999..3017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3035..3053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3070..3088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3106..3124
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3142..3160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3177..3195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3213..3231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3243..3281
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3278..3302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3377..4588
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4425..4444
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6138..6191
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6195..6197,6198..6911)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6237..6258)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6498..6517)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6845..6866
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6971..7559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7024..7044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7442..7453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7562..7578)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7563..7579)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8088..8268
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8294..8311)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8300..8524
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8570..8998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8657..8676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8867..8888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9007..9027)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9012..9341
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9192..9327
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9254..9273
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9389..9436
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9455..9829
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9959..10080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9996..10015)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10050..10069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10129..10145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10129..10145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10142..10164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10153..10169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10177..10183,10184..10201,10202..10207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10222..10243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10360..10377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10531..11119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10611..10630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11290..11894,1..187,188..256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
       61 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
      121 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
      181 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
      241 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
      301 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      361 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
      421 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
      481 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
      541 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
      601 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      661 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      721 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      781 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      841 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      901 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      961 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     1021 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     1081 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     1141 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     1201 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     1261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     1321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     1381 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     1441 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     1501 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     1561 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1621 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1681 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1741 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1801 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1861 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1921 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1981 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     2041 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     2101 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     2161 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     2221 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     2281 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     2341 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     2401 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     2461 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     2521 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     2581 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2641 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2701 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2761 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2821 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2881 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2941 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattct agttttactc
     3001 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgatgt
     3061 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     3121 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc
     3181 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     3241 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
     3301 tcgcctggag aattcgttaa cggatcctct agagctcagc ctcgagattt aaatttaatt
     3361 aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     3421 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     3481 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     3541 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     3601 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     3661 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     3721 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     3781 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     3841 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     3901 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     3961 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     4021 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     4081 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     4141 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     4201 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     4261 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     4321 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     4381 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     4441 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     4501 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     4561 ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga tctccacccc
     4621 gtaccggtta acccatgtct cgctccgtgg ccttagctgt gctcgcgcta ctctctcttt
     4681 ctggcctgga ggctgtgatg gcgccgagaa cgctaatact aggatgcggt gctagcggtg
     4741 gtggtggtag cggaggtgga ggcagcatcc agcgtactcc aaagattcag gtttactcac
     4801 gtcatccagc agagaatgga aagtcaaatt tcctgaattg ctatgtgtct gggtttcatc
     4861 catccgacat tgaagttgac ttactgaaga atggagagag aattgaaaaa gtggagcatt
     4921 cagacttgtc tttcagcaag gactggtctt tctatctctt gtactacact gaattcaccc
     4981 ccactgaaaa agatgagtat gcctgccgtg tgaaccatgt gactttgtca cagcccaaga
     5041 tagttaagtg ggatcgagac atgggcggtg gtggttccgg tggaggcggt tccggaggtg
     5101 gtggatccgg tggtggaggt agtggctccc actccttgaa gtatttccac acttccgtgt
     5161 cccggcccgg ccgcggggag ccccgcttca tctctgtggg ctacgtggac gacacccagt
     5221 tcgtgcgctt cgacaacgac gccgcgagtc cgaggatggt gccgcgggcg ccgtggatgg
     5281 agcaggaggg gtcagagtat tgggaccggg agacacggag cgccagggac accgcacaga
     5341 ttttccgagt gaatctgcgg acgctgcgcg gctgctacaa tcagagcgag gccgggtctc
     5401 acaccctgca gtggatgcat ggctgcgagc tggggcccga cgggcgcttc ctccgcgggt
     5461 atgaacagtt cgcctacgac ggcaaggatt atctcaccct gaatgaggac ctgcgctcct
     5521 ggaccgcggt ggacacggcg gctcagatct ccgagcaaaa gtcaaatgat gcctctgagg
     5581 cggagcacca gagagcctac ctggaagaca catgcgtgga gtggctccac aaatacctgg
     5641 agaaggggaa ggagacgctg cttcacctgg agcccccaaa gacacacgtg actcaccacc
     5701 ccatctctga ccatgaggcc accctgaggt gctgggccct gggcttctac cctgcggaga
     5761 tcacactgac ctggcagcag gatggggagg gccataccca ggacacggag ctcgtggaga
     5821 ccaggcctgc aggggatgga accttccaga agtgggcagc tgtggtggtg ccttctggag
     5881 aggagcagag atacacgtgc catgtgcagc atgaggggct acccgagccc gtcaccctga
     5941 gatggaagcc ggcttcccag cccaccatcc ccatcgtggg catcattgct ggcctggttc
     6001 tccttggatc tgtggtctct ggagctgtgg ttgctgctgt gatatggagg aagaagagct
     6061 caggtggaaa aggagggagc tactctaagg ctgagtggag cgacagtgcc caggggtctg
     6121 agtctcacag cttgggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     6181 atcccggccc tgtggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     6241 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6301 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6361 cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg
     6421 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     6481 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     6541 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     6601 tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca
     6661 agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg
     6721 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6781 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6841 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6901 tgtacaagta atgtacaagt aaagcggccg cgactctagt ccctcccaat tcgatatcaa
     6961 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7021 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7081 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7141 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7201 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7261 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7321 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7381 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7441 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7501 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7561 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     7621 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7681 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7741 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7801 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     7861 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     7921 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     7981 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     8041 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     8101 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8161 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8221 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     8281 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     8341 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     8401 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     8461 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     8521 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     8581 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8641 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8701 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8761 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8821 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8881 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8941 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     9001 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9061 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     9121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     9181 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9241 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     9301 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     9361 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     9421 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     9481 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     9541 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     9601 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     9661 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     9721 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     9781 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     9841 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9901 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9961 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10021 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10081 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    10141 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10201 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10261 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10321 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11041 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11101 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11161 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11221 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11281 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11341 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11401 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11461 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11521 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11581 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11641 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11701 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11761 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11821 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11881 accgagttgc tctt
//
