LOCUS       sequence-409739-       11703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for expression of mouse Crry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: TRE-UBC-Crry-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..11703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2610..2628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2646..2664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2681..2699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2717..2735
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2788..2806
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2824..2842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2854..2892
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2889..2913
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2988..4199
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4036..4055
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(4862..4873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5558..5611
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5615..5617,5618..6331)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5657..5678)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5918..5937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6265..6286
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6391..6979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6444..6464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6862..6873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6982..6998)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6983..6999)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7508..7688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7714..7731)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7720..7944
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7990..8418
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8077..8096)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8287..8308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8427..8447)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8432..8761
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8612..8747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8674..8693
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8809..8856
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8875..9249
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9379..9500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9416..9435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9470..9489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9549..9565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9549..9565)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9562..9584)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9573..9589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9597..9603,9604..9621,9622..9627))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9642..9663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9780..9797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9951..10539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10031..10050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10710..11501,11502..11570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11333..11352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11571..11675)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagttttact ccctatcagt gatagagaac gtatgtcgag
     2641 tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa
     2701 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     2761 agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt
     2821 tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat
     2881 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattcgtta acggatcctc
     2941 tagagctcag cctcgagatt taaatttaat taacccaccc aagatctggc ctccgcgccg
     3001 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     3061 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3121 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3181 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     3241 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3301 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3361 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3421 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3481 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     3541 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     3601 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     3661 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     3721 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     3781 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     3841 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     3901 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     3961 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     4021 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     4081 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     4141 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     4201 aagcttggta ccgagctcgg atctccaccc cgtaatggaa gtgtcctcca gatccagcga
     4261 gcctctggat cccgtgtggc tcctggtcgc cttcggaaga ggaggcgtca agctggaggt
     4321 cctgctgctg tttctgctcc ccttcacact gggccactgt cctgccccta gccaactgcc
     4381 ctccgccaag cctatcaacc tcaccgatga gtccatgttt cccattggca cctacctcct
     4441 gtatgagtgc ctccctggat atattaagag acagttctcc atcacatgca agcaggattc
     4501 cacctggacc agcgccgaag acaagtgcat caggaagcag tgcaaaaccc cttccgatcc
     4561 cgaaaatgga ctcgtgcacg tgcacacagg aatccagttc ggctccagaa ttaattatac
     4621 atgtaatcag ggctacagac tgatcggcag ctccagcgct gtctgtgtga ttaccgatca
     4681 gagcgtggat tgggacaccg aggcccctat ctgtgagtgg atcccttgcg agattccccc
     4741 tggcattcct aacggcgact tctttagctc caccagagag gatttccact acggcatggt
     4801 cgtcacctac aggtgcaaca cagacgccag gggaaaagcc ctgttcaacc tggtcggcga
     4861 gccttccctc tattgcacat ccaatgacgg cgaaatcggc gtctggagcg gacctccccc
     4921 ccaatgcatc gaactgaaca agtgcacacc ccccccttat gtggagaatg ccgtcatgct
     4981 cagcgaaaat aggtccctgt tttccctgag ggacatcgtg gagttcaggt gtcaccctgg
     5041 cttcatcatg aagggagcca gcagcgtgca ttgccagagc ctcaataagt gggagcccga
     5101 gctgccctcc tgcttcaagg gcgtcatttg cagactgccc caggagatga gcggcttcca
     5161 aaagggcctc ggcatgaaga aagagtacta ctatggcgaa aatgtcaccc tggaatgcga
     5221 ggatggctat acactcgagg gcagctccca gagccagtgc cagagcgatg gcagctggaa
     5281 ccctctcctg gctaagtgcg tgtccaggag catcagcggc ctgattgtgg gcatctttat
     5341 tggaattatt gtcttcatcc tcgtgatcat tgtgtttatc tggatgattc tgaagtacaa
     5401 aaaaagaaac accacagatg aaaagtataa agaggtgggc attcatctca actacaagga
     5461 ggatagctgc gtgagactgc agtccctgct cacatcccaa gaaaatagca gcaccacatc
     5521 ccccgccaga aattccctca cccaagaggt gtccttagag ggcagaggaa gtcttctaac
     5581 atgcggtgac gtggaggaga atcccggccc tgtggtgagc aagggcgagg agctgttcac
     5641 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5701 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5761 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
     5821 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5881 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5941 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     6001 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     6061 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
     6121 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6181 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     6241 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6301 cactctcggc atggacgagc tgtacaagta atgtacaagt aaagcggccg cgactctagt
     6361 ccctcccaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     6421 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6481 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6541 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6601 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6661 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6721 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6781 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6841 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6901 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6961 ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg
     7021 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     7081 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     7141 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7201 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     7261 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     7321 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     7381 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     7441 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     7501 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7561 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7621 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7681 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     7741 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7801 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7861 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7921 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     7981 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     8041 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     8101 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8161 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8221 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8281 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8341 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8401 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8461 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8521 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8581 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8641 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8701 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8761 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     8821 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     8881 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     8941 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     9001 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     9061 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     9121 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     9181 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     9241 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     9301 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     9361 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9421 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9481 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     9541 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9601 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9661 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11701 gac
//
